Composition for enhancing carbonic anhydrase activity comprising imidazole and trehalose and method for capturing carbon dioxide using the same

Abstract

The present invention relates to a composition for enhancing carbonic anhydrase activity comprising imidazole and trehalose and a method for capturing carbon dioxide using the same, and more specifically, to a composition for enhancing carbonic anhydrase activity comprising carbonic anhydrase, imidazole, trehalose and sucrose and a method for capturing carbon dioxide using the same.

Description

Technology Field

[0001] The present invention relates to a composition for enhancing carbonic anhydrase activity comprising imidazole and trehalose and a method for capturing carbon dioxide using the same, and more specifically, to a composition for enhancing carbonic anhydrase activity comprising carbonic anhydrase, imidazole, trehalose and sucrose and a method for capturing carbon dioxide using the same. Background Technology

[0003] Global warming is known to be primarily caused by carbon dioxide and various greenhouse gases generated during the combustion of fossil fuels.

[0004] Carbon dioxide in the atmosphere absorbs infrared radiation emitted from the Earth, causing the Earth's temperature to rise and leading to many environmental problems.

[0005] Technologies developed to date for carbon dioxide treatment include physical methods such as adsorption and membrane separation, as well as methods that store captured carbon dioxide in lipids using chemical means.

[0006] In particular, carbon dioxide capture methods using chemical means are being extensively studied, but this method requires a large amount of energy to increase the carbon dioxide hydration rate, which presents the problem of being uneconomical.

[0007] Meanwhile, carbonic anhydrase (CA) is known as a catalyst that hydrates carbon dioxide molecules, and the hydrated carbon dioxide molecules exist in the form of bicarbonate.

[0008] The above enzyme may be immobilized on a specific support to enable easy separation from the reaction mixture of a biochemical process or to increase the stability of the enzyme.

[0009] However, the above enzyme immobilization method has problems such as a complex reaction process, secondary contamination, and loss of enzyme activity.

[0010] Therefore, it is necessary to develop technology that can increase the hydration rate of carbon dioxide by improving the activity and thermal stability of carbonic anhydrase. Prior art literature

[0012] Korean Published Patent No. 10-2023-0142956, Korean Registered Patent No. 10-2658830 The problem to be solved

[0013] The present invention aims to solve the problems of the prior art described above by providing a composition for enhancing carbonic anhydrase activity that can increase the hydration rate of carbon dioxide by improving the activity and thermal stability of carbonic anhydrase. means of solving the problem

[0015] To achieve the above objectives, the present invention provides a composition for enhancing carbonic anhydrase activity comprising carbonic anhydrase, imidazole, trehalose, and sucrose.

[0016] In one embodiment of the present invention, the content of the imidazole is characterized as being 1 to 50 mM.

[0017] In one embodiment of the present invention, the content of the trehalose is characterized as being 10 to 500 mM.

[0018] In one embodiment of the present invention, the sucrose content is characterized as being 10 to 500 mM.

[0019] In one embodiment of the present invention, the composition is characterized by additionally comprising 1 to 20 mM of 1-methylimidazole.

[0020] In addition, the present invention provides a method for capturing carbon dioxide comprising the step of reacting the carbonic anhydrase activity-enhancing composition with a carbon dioxide-saturated solution. Effects of the invention

[0022] The present invention can provide a composition for enhancing carbonic anhydrase activity that can increase the hydration rate of carbon dioxide by improving the activity and thermal stability of carbonic anhydrase. Specific details for implementing the invention

[0024] The present invention will be described in detail below based on the following examples. The terms, examples, etc. used in the present invention are merely illustrative to explain the invention more specifically and to aid the understanding of those skilled in the art, and the scope of the rights, etc., of the present invention should not be interpreted as being limited thereto.

[0025] Unless otherwise defined, technical and scientific terms used in this invention represent the meanings commonly understood by those skilled in the art to which this invention pertains.

[0027] The present invention relates to a composition for enhancing carbonic anhydrase activity comprising carbonic anhydrase (CA), imidazole, trehalose, and sucrose.

[0029] The above-mentioned carbonic anhydrase is an enzyme that catalyzes the conversion of carbon dioxide and water into bicarbonate ions and hydrogen ions.

[0030] One or more carbonic anhydrases of the α, β, γ, δ, ε types may be used as the above carbonic anhydrase.

[0031] For example, α-type carbonic anhydrase (ngCA) derived from the gonococcus Neisseria gonorrhoeae can be used.

[0032] It is preferable that the content of the carbonic anhydrase be 1 to 100 μg / ml, and when the content satisfies the above numerical range, the activity and thermal stability of the carbonic anhydrase can be maximized.

[0034] The content of the above imidazole is preferably 1 to 50 mM, and when the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0036] The content of the above trehalose is 10 to 500 mM, and when the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0038] The content of the above sucrose is preferably 10 to 500 mM, and when the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0040] In addition, the weight ratio of the trehalose and sucrose is preferably 60 to 80:20 to 40, and when the weight ratio satisfies the above numerical range, the activity and thermal stability of the carbonic anhydrase can be maximized.

[0042] The composition of the present invention may include a solvent, and the solvent may include Tris-HCl buffer solution (pH 7-9), distilled water, etc.

[0044] In addition, the above composition may further include 1 to 20 mM of 1-methylimidazole, and if the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0046] In addition, the above composition may further include 1 to 20 mM of 2-methylimidazole, and when the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0048] In addition, the above composition may further include 1 to 20 mM of dextrin, and if the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0050] In addition, the above composition may further include 1 to 20 mM of histidine, and if the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0052] In addition, the above composition may further include 1 to 10 mM of 4-methylimidazole and 1 to 10 mM of polyethylene glycol, and when the content satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0054] In addition, the above composition may be used by mixing 1-methylimidazole, 2-methylimidazole, and 4-methylimidazole, wherein the weight ratio of 1-methylimidazole, 2-methylimidazole, and 4-methylimidazole is preferably 100:60 to 80:20 to 40, and when the weight ratio satisfies the above numerical range, the activity and thermal stability of carbonic anhydrase can be maximized.

[0056] In addition, the present invention relates to a method for capturing carbon dioxide comprising the step of reacting the carbonic anhydrase activity-enhancing composition with a carbon dioxide solution or a carbon dioxide-saturated solution.

[0057] The composition for enhancing carbonic anhydrase activity of the present invention can increase the hydration rate of carbon dioxide by improving the activity and thermal stability of carbonic anhydrase.

[0059] The present invention will be described in detail below through examples and comparative examples. The following examples are merely illustrative for the implementation of the present invention, and the scope of the present invention is not limited by the following examples.

[0061] (Example 1)

[0062] A composition for enhancing carbonic anhydrase activity was prepared by mixing Tris-HCl buffer solution (pH 7.5), 10 μg / ml of α-type CA (ngCA) from Neisseria gonorrhoeae, 10 mM of imidazole, 140 mM of trehalose, and 60 mM of sucrose.

[0064] (Example 2)

[0065] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that 0.5 mM of imidazole was used.

[0067] (Example 3)

[0068] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that 80 mM of imidazole was used.

[0070] (Example 4)

[0071] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that 100 mM of trehalose and 100 mM of sucrose were used.

[0073] (Example 5)

[0074] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that 180 mM of trehalose and 20 mM of sucrose were used.

[0076] (Example 6)

[0077] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that 5 mM of 1-methylimidazole and 5 mM of 2-methylimidazole were additionally used.

[0079] (Comparative Example 1)

[0080] A composition for enhancing carbonic anhydrase activity was prepared in the same manner as in Example 1, except that imidazole, trehalose, and sucrose were not used.

[0082] (Carbon dioxide conversion rate)

[0083] The conversion rate of carbon dioxide to calcium carbonate for a composition for enhancing carbonic anhydrase activity was determined using the calcium carbonate precipitation method.

[0084] A saturated aqueous solution of carbon dioxide was prepared by bubbling carbon dioxide into water.

[0085] 5 mL of a carbon dioxide-saturated aqueous solution was added to 5 mL of the carbonic anhydrase activity-enhancing composition, and the reaction was carried out for 10 minutes.

[0086] The weight of the precipitated calcium carbonate (CaCO3) was measured.

[0088] (Thermal stability)

[0089] The thermal stability of the composition for enhancing carbonic anhydrase activity was evaluated.

[0090] After storing the above composition for enhancing carbonic anhydrase activity at 50°C for 12 hours, the weight of the precipitate was measured. The weight loss rate of the precipitate was calculated by comparing the weight of the precipitate after storage at 50°C for 12 hours with the weight of the initial precipitate.

[0092] The carbon dioxide conversion rate and thermal stability of the carbonic anhydrase activity-enhancing compositions prepared from the above examples and comparative examples were evaluated and are shown in Table 1.

[0094] Weight of precipitate (mg) Weight loss of precipitate (%) Example 1 5.6 21 Example 2 2.9 32 Example 3 3.2 35 Example 4 2.7 29 Example 5 3.3 33 Example 6 7.0 14 Comparative Example 1 2.5 41

[0096] From the results of Table 1 above, it can be seen that Examples 1 to 6 have excellent carbon dioxide conversion rates and thermal stability, and in particular, Examples 1 and 6 have the best characteristics.

[0097] On the other hand, it can be seen that Comparative Example 1 has inferior characteristics compared to the Example.

Claims

Claim 1 A composition for enhancing carbonic anhydrase activity comprising carbonic anhydrase, imidazole, trehalose, and sucrose. Claim 2 In paragraph 1, A composition for enhancing carbonic anhydrase activity, characterized in that the content of the above-mentioned imidazole is 1 to 50 mM. Claim 3 In paragraph 2, A composition for enhancing carbonic anhydrase activity, characterized in that the content of the trehalose is 10 to 500 mM. Claim 4 In paragraph 3, A composition for enhancing carbonic anhydrase activity, characterized by the sucrose content being 10 to 500 mM. Claim 5 In paragraph 4, A composition for enhancing carbonic anhydrase activity, characterized by further comprising 1 to 20 mM of 1-methylimidazole. Claim 6 A method for capturing carbon dioxide comprising the step of reacting the carbonic anhydrase activity-enhancing composition of claim 1 with a carbon dioxide-saturated solution.

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