Guide module for binding between nucleic acid and magnetic bead

Abstract

The present invention relates to a binding induction module between nucleic acid and magnetic beads, and more specifically, to a binding induction module between nucleic acid and magnetic beads that can be utilized when extracting nucleic acid using magnetic beads. A binding induction module between nucleic acid and magnetic beads according to one embodiment of the present invention is characterized by comprising: a solution receiving portion that receives a mixed solution in which nucleic acid and magnetic beads float; a strip portion formed with an open top and formed to be inserted into or withdrawn from the solution receiving portion; a magnetic rod portion that has magnetism and is formed to be inserted into and withdrawn from the internal space of the strip portion; a magnetic block portion formed to selectively apply a magnetic field to the solution receiving portion; and a control portion that controls the movement of the strip portion and the magnetic rod portion.

Description

Technology Field

[0001] The present invention relates to a binding induction module between nucleic acid and magnetic beads, and more specifically, to a binding induction module between nucleic acid and magnetic beads that can be utilized in nucleic acid extraction using magnetic beads. Background Technology

[0002] The technology for extracting nucleic acids (or biological substances) from biological samples such as blood and tissues is widely used in various fields such as molecular diagnostics, virus testing, forensics, biology, genomics, and biochemistry.

[0003] Methods for extracting nucleic acids from biological samples can be broadly divided into three categories: separating nucleic acids adsorbed by magnets using magnetic beads, eluting the solution using a column by applying air to the column to push out the solution, and eluting the solution by centrifuging the column itself.

[0004] The currently widely used nucleic acid extraction method using magnetic beads extracts nucleic acids by sequentially performing a lysis process, in which the cell walls of the sample are dissolved in a lysis buffer capable of dissolving biological samples to release nucleic acids; a binding process, in which nucleic acids are combined with magnetic beads; a washing process, in which impurities other than nucleic acids are removed; and an eluting process, in which the magnetic beads are separated from the nucleic acids.

[0005] In the binding process for combining nucleic acids and magnetic beads, a process of inducing flow in the solution is performed to rapidly bind the nucleic acids and magnetic beads suspended in the lysis solution. However, since the magnetic beads and nucleic acids move together in the same direction during solution flow, there is a limitation in that the effect of shortening the binding time between the nucleic acids and magnetic beads is not significant.

[0006] Therefore, there was a demand for a binding induction module that enables the binding between nucleic acids and magnetic beads to occur in a relatively short time. The problem to be solved

[0008] The technical problem that the present invention aims to solve is to provide a binding induction module that enables binding between nucleic acids and magnetic beads to occur in a relatively short time.

[0009] The technical problems that the present invention aims to solve are not limited to those mentioned above, and other unmentioned technical problems will be clearly understood by those skilled in the art to which the present invention belongs from the description below. means of solving the problem

[0010] To achieve the above technical objective, the present invention provides a binding induction module between nucleic acid and magnetic beads, comprising: a solution receiving portion for receiving a mixed solution in which nucleic acid and magnetic beads float; a strip portion formed with an open top and capable of being inserted into and withdrawn from the solution receiving portion; a magnetic rod portion having magnetism and capable of being inserted into and withdrawn from the internal space of the strip portion; a magnetic block portion formed to selectively apply a magnetic field to the solution receiving portion; and a control portion that controls the movement of the strip portion and the magnetic rod portion, respectively, and controls the magnetic block portion for the selective application of a magnetic field to the solution receiving portion, wherein the movement of the magnetic beads is restricted by the influence of the magnetic field of the magnetic block portion even when the mixed solution flows due to the movement of the strip portion.

[0011] In one embodiment of the present invention, the magnetic block portion is formed of a permanent magnet, and a magnetic field can be selectively applied to the solution receiving portion by adjusting the distance from the solution receiving portion.

[0012] In one embodiment of the present invention, the control unit may combine nucleic acid and magnetic beads by repeatedly performing the insertion and withdrawal of the strip portion a predetermined number of times, and may reinforce the combination of the nucleic acid-magnetic bead conjugate by moving the magnetic block portion to be located within a certain distance of the solution receiving portion.

[0013] In one embodiment of the present invention, the magnetic block portion is formed as an electromagnet and can selectively apply a magnetic field to the solution receiving portion depending on whether current is supplied.

[0014] In one embodiment of the present invention, the magnetic block portion is located in the opposite direction to the magnetic rod portion and may be formed to have a larger area than the magnetic rod portion. Effects of the invention

[0015] According to an embodiment of the present invention, since the movement of magnetic beads in a mixed solution is restricted by the magnetic field of a magnetic block, the probability of contact between the nucleic acid and the magnetic block increases during the flow of the mixed solution, and binding between the nucleic acid and the magnetic beads can be formed quickly.

[0016] The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description of the invention or the claims. Brief explanation of the drawing

[0017] FIG. 1 is a front view of a binding induction module between nucleic acid and magnetic beads according to one embodiment of the present invention. FIG. 2 is a block diagram of a binding induction module between nucleic acid and magnetic beads according to one embodiment of the present invention. FIG. 3 is a reference diagram illustrating a method of using a binding induction module between nucleic acid and magnetic beads according to one embodiment of the present invention. Specific details for implementing the invention

[0018] The present invention will be described below with reference to the attached drawings. However, the present invention may be implemented in various different forms and is therefore not limited to the embodiments described herein. Furthermore, in order to clearly explain the present invention in the drawings, parts unrelated to the explanation have been omitted, and similar parts throughout the specification have been given similar reference numerals.

[0019] Throughout the specification, when it is stated that a part is "connected (connected, in contact, combined)" with another part, this includes not only cases where they are "directly connected," but also cases where they are "indirectly connected" with other members interposed between them. Furthermore, when it is stated that a part "includes" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but rather allows for the inclusion of additional components.

[0020] The terms used herein are merely for describing specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as “comprising” or “having” are intended to indicate the presence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0021] FIG. 1 is a front view of a binding induction module (1) between a nucleic acid and a magnetic bead according to one embodiment of the present invention, and FIG. 2 is a block diagram of a binding induction module (1) between a nucleic acid and a magnetic bead according to one embodiment of the present invention.

[0022] A binding induction module (1, hereinafter referred to as the binding induction module) between nucleic acid and magnetic beads is intended to shorten the binding time between nucleic acid (N) and magnetic beads (M) and can be utilized during nucleic acid extraction using magnetic beads. At this time, the binding induction module (1) may constitute part of a nucleic acid extraction device using magnetic beads or may be provided separately.

[0023] The coupling induction module (1) may include a solution receiving section (10) for receiving a mixed solution, a strip section (20) for generating a flow of the mixed solution (L) within the solution receiving section (10), a magnetic rod section (not shown) provided to be insertable into the strip section (20) for applying a magnetic field to the solution receiving section (10) according to the distance from the solution receiving section (10), a magnetic block section (30) for selectively applying a magnetic field to the solution receiving section (10), and a control section (40) for controlling the overall operation of the coupling induction module (1).

[0024] The solution receiving portion (10) can receive a mixed solution (L) in which nucleic acid (N) and magnetic beads (M) are suspended. The mixed solution (L) can be formed by mixing magnetic beads (M) and a biological sample in a lysis solution. At this time, the cell wall of the biological sample is decomposed by the lysis solution, and the nucleic acid can be separated.

[0025] The solution receiving portion (10) may have a receiving space (11) with an open top for receiving the mixed solution (L). The receiving space (11) may be formed to have a constant cross-sectional area or may be formed to have a cross-sectional area that gradually increases from the bottom to the top.

[0026] The strip portion (20) is located above the solution receiving portion (10) and can be formed to be inserted into and withdrawn from the solution receiving portion (10). Specifically, the strip portion (20) may include a bore (21) in the form of a circular or polygonal column with an open top and formed to extend long in the vertical direction, and a first moving means (22) for moving the bore (21). The bore (21) and the first moving means (22) may be detachably coupled.

[0027] The magnetic rod portion is located on the upper side of the strip portion (20) and can be formed to be inserted into and withdrawn from the strip portion (20). Specifically, the magnetic rod portion may include a magnet (not shown) that forms magnetism and a second moving means (23) that moves the magnet. At this time, the magnet may be a permanent magnet, but is not limited thereto.

[0028] The magnetic block portion (30) can exert a magnetic field effect on the magnetic bead (M) located within the solution receiving portion (10). The magnetic block portion (30) can be configured to selectively apply a magnetic field to the solution receiving portion.

[0029] Additionally, the magnetic block portion (30) may be positioned opposite to the magnetic load portion and may be formed to have a larger area than the magnetic load portion. The magnetic block portion (30) may be formed to the area level of a 96-well plate having a 12X8 well array, but is not limited thereto.

[0030] The control unit (40) can control the insertion and withdrawal of the strip unit (20) into the solution receiving unit (10). Specifically, the control unit (40) can be connected to the first moving means (22) and can control the first moving means (22) so that the flow of the mixed solution (L) within the solution receiving unit (10) is generated by the insertion and withdrawal of the strip unit (20). The control unit (40) can control the first moving means (22) to repeat the insertion and withdrawal of the strip unit (20) a predetermined number of times.

[0031] Meanwhile, the first moving means (22) can receive current from the power supply unit (50), and the supply and cutoff of current from the power supply unit (50) to the first moving means (22) can be performed by the control unit (40).

[0032] The control unit (40) can control the insertion and withdrawal of the magnetic rod unit for the strip unit (20). Specifically, the control unit (40) can be connected to the second moving means (23) and can control the second moving means (23) so that the magnetic beads (M) of the mixed solution are coupled or released by the insertion and withdrawal of the magnetic rod unit.

[0033] The control unit (40) can withdraw the magnetic load unit from the strip unit (20) so that the magnetic bead (M) is not affected by the magnetic load unit during the process of generating the flow of the mixed solution (L) by the insertion and withdrawal of the strip unit (20).

[0034] The control unit (40) can move the magnetic rod unit into the strip so that the magnetic bead (M) is attached to the surface of the strip unit (20) during the process of moving the magnetic bead.

[0035] Accordingly, in the present invention, when the mixed solution flows, the nucleic acid (N) flows together with the flow of the mixed solution (L), but the movement of the magnetic bead (M) may be restricted due to the influence of the magnetic field of the magnetic block part (30). That is, in the present invention, due to the moving nucleic acid (N) and the magnetic bead (M) whose movement is restricted, the probability of contact between the nucleic acid (N) and the magnetic block (M) can be increased, and thus, the binding between the nucleic acid (N) and the magnetic bead (M) can be formed quickly.

[0036] According to one embodiment of the present invention, the magnetic block portion (30) may be formed of a permanent magnet and may selectively apply a magnetic field to the solution receiving portion (10) by adjusting the distance from the solution receiving portion (10). In this case, when the mixed solution flows due to the upward and downward movement of the strip portion (20), the magnetic block portion (30) may be moved to be within a certain distance from the solution receiving portion (10) to restrict the movement of the magnetic beads (M) of the mixed solution (L).

[0037] When the magnetic block portion (30) is located within a certain distance from the solution receiving portion (10), it can exert a magnetic field effect on the magnetic bead (M) located within the solution receiving portion (10), and when it is located beyond a certain distance from the solution receiving portion (10), it cannot exert a magnetic field effect on the magnetic bead (M) located within the solution receiving portion (10).

[0038] According to another embodiment of the present invention, the magnetic block portion (30) may be formed as an electromagnet and may selectively apply a magnetic field to the solution receiving portion (10) depending on whether current is supplied. In this case, current may be applied to the magnetic block portion (30) to restrict the movement of the magnetic beads (M) of the mixed solution (L) during the flow of the mixed solution due to the lifting motion of the strip portion (20).

[0039] When current is applied, the magnetic block (30) can exert a magnetic field effect on the magnetic bead (M) located within the solution receiving section (10), and when current is not applied, it cannot exert a magnetic field effect on the magnetic bead (M) located within the solution receiving section (10).

[0040] Meanwhile, the control unit (40) can combine nucleic acid (N) and magnetic beads (M) to form a nucleic acid-magnetic conjugate (C), and then introduce a magnetic rod unit into the strip unit (20) introduced into the solution receiving unit (10) so that the nucleic acid-magnetic bead conjugate (C) is attached to the surface of the strip unit (20) by the influence of the magnetic field from the magnetic rod unit.

[0041] FIG. 3 is a reference diagram illustrating a method of using a binding induction module (1) between nucleic acid and magnetic beads according to one embodiment of the present invention.

[0042] As shown in FIG. 3(a), a mixed solution (L) in which nucleic acid (N) and magnetic beads (M) are suspended is received in a solution receiving section (10). The strip section (20) may be withdrawn from the solution receiving section (10) to facilitate the smooth movement of the mixed solution (L) received from the outside into the solution receiving section (10), but is not limited thereto.

[0043] The magnetic block portion (30) can be positioned within a certain distance from the solution receiving portion (10).

[0044] Afterwards, as shown in Fig. 3(b), the strip portion (20) is moved up and down, and the insertion and withdrawal of the strip portion (20) into and out of the solution receiving portion (10) are repeated to cause flow in the mixed solution (L).

[0045] At this time, the nucleic acid (N) flows together with the flow of the mixed solution (L), while the movement of the magnetic bead (M) is restricted by the influence of the magnetic field of the magnetic block part (30). Accordingly, the probability of contact between the nucleic acid (N) and the magnetic bead (M) increases, and the binding between the nucleic acid (N) and the magnetic bead (M) can be formed quickly.

[0046] Afterwards, as shown in Fig. 3 (c), the magnetic block (30) is moved away from the solution receiving part (10) so that the magnetic field of the magnetic block (30) does not affect the solution receiving part (10), thereby preventing the magnetic field of the magnetic block (30) from affecting the solution receiving part (10).

[0047] Afterwards, as shown in Fig. 3 (d), a magnetic rod portion is introduced into the strip portion (20) so that a nucleic acid-magnetic bead conjugate (C) is attached to the surface of the strip portion (20).

[0048] Afterward, as shown in FIG. 3 (e), the strip portion (20) and the magnetic rod portion are moved upward simultaneously to separate the nucleic acid-magnetic bead conjugate (C) from the mixed solution (L). At this time, the separated nucleic acid-magnetic bead conjugate (C) can be moved to a module that processes a washing process.

[0049] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will understand that other specific forms can be easily modified without altering the technical spirit or essential features of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. For example, each component described as a single unit may be implemented in a distributed manner, and components described as distributed may likewise be implemented in a combined form.

[0050] The scope of the present invention is defined by the claims set forth below, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof should be interpreted as being included within the scope of the present invention. Explanation of the symbols

[0051] 1: Module for inducing binding between nucleic acids and magnetic beads 10: Solution receiving section 20: Strip section 30: Magnetic block part 40 : Control unit 50 : Power supply

Claims

Claim 1 A binding induction module between nucleic acid and magnetic beads, comprising: a solution receiving portion for receiving a mixed solution in which nucleic acid and magnetic beads float; a strip portion formed with an open top and capable of being inserted into and withdrawn from the solution receiving portion; a magnetic rod portion having magnetism and capable of being inserted into and withdrawn from the internal space of the strip portion; a magnetic block portion formed to selectively apply a magnetic field to the solution receiving portion; and a control portion that controls the movement of the strip portion and the magnetic rod portion, respectively, and controls the magnetic block portion for the selective application of a magnetic field to the solution receiving portion, wherein the movement of the magnetic beads is restricted by the influence of the magnetic field of the magnetic block portion even during the flow of the mixed solution caused by the movement of the strip portion. Claim 2 A binding induction module between nucleic acid and magnetic beads according to claim 1, wherein the magnetic block portion is formed of a permanent magnet and selectively applies a magnetic field to the solution receiving portion by adjusting the distance from the solution receiving portion. Claim 3 A binding induction module between a nucleic acid and a magnetic bead according to paragraph 2, wherein the control unit performs the insertion and withdrawal of the strip portion repeatedly a predetermined number of times to bind the nucleic acid and the magnetic bead, and moves the magnetic block portion to be located within a certain distance of the solution receiving portion to reinforce the binding of the nucleic acid-magnetic bead conjugate. Claim 4 A binding induction module between nucleic acid and magnetic beads according to claim 1, wherein the magnetic block portion is formed as an electromagnet and selectively applies a magnetic field to the solution receiving portion depending on whether current is supplied. Claim 5 A binding induction module between nucleic acid and magnetic beads according to claim 1, wherein the magnetic block portion is located in a direction opposite to the magnetic rod portion and is formed to have a larger area than the magnetic rod portion.

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