Production and Application of Growth Medium for Cultivated Meat using Slaughter Blood

Abstract

The objective of the present invention is to provide a technology for manufacturing a culture medium for producing cultured meat using serum extracted from the blood of slaughtered Hanwoo cattle. To this end, the present invention provides a method for ensuring the stability of Hanwoo serum and optimizing the composition of the medium containing nutrients and growth factors necessary for the production of cultured meat.

Description

Technology Field

[0001] The present invention belongs to the fields of biotechnology and food engineering, and more specifically, relates to a technology for manufacturing a culture medium suitable for producing cultured meat using serum extracted from the blood of slaughtered Hanwoo cattle. Background Technology

[0002] In Korea, more than 100,000 cattle are slaughtered annually, and most of the blood byproducts generated during this process are discarded. This results in additional processing costs, such as chemical treatment fees and water usage fees for dilution, and contributes to environmental pollution. Due to the lack of utilization methods for slaughter byproducts, economic burdens and environmental issues persist.

[0003] The cultured meat industry is garnering attention as an innovative alternative to address global food demand and environmental issues. However, the production of culture media, a core component of cultured meat production, entails high costs, which drives up the unit price of cultured meat and limits the industry's scalability. In particular, Fetal Bovine Serum (FBS), which accounts for the highest cost among media components, is effective for cell proliferation and differentiation due to its inclusion of growth factors and hormones; however, ethical issues are raised regarding its production process. Despite these challenges, there is currently a lack of distinct substitutes for FBS, leading to a heavy reliance on imports domestically. Consequently, the resulting supply difficulties and economic burdens remain unresolved.

[0004] Therefore, developing an economical and eco-friendly culture medium specifically for meat production by utilizing Hanwoo blood discarded during the slaughtering process can serve as a strategic solution to reduce dependence on imported bovine fetal serum-based media while simultaneously increasing the added value of the domestic livestock industry. To achieve this, securing efficient and safe pretreatment technologies, establishing optimal conditions for media supplements, and conducting standardization research are essential. The problem to be solved

[0005] The objective of the present invention is to provide a technology for manufacturing a culture medium for producing cultured meat using serum extracted from the blood of slaughtered Hanwoo cattle. To this end, the present invention provides a method for ensuring the stability of Hanwoo serum and optimizing the composition of the medium containing nutrients and growth factors necessary for the production of cultured meat. means of solving the problem

[0006] To achieve the above objective, the present invention provides a culture medium composition for producing cultured meat using Hanwoo serum extracted from slaughtered Hanwoo blood.

[0007] In one embodiment of the present invention, the container used for collecting Hanwoo blood is preferably 1L, but is not limited thereto.

[0008] In one embodiment of the present invention, Dulbecco's Modified Eagle Medium (DMEM) is preferred as the basic culture medium composition condition to be mixed with Hanwoo serum, but is not limited thereto.

[0009] In one embodiment of the present invention, the concentration of Hanwoo serum in the medium may be 50% (v / v) to 40% (v / v) to 30% (v / v) to 20% (v / v) to 10% (v / v) based on the total volume of the medium.

[0010] In one embodiment of the present invention, the type of cell used for culture may be of animal origin, and the animal includes not only humans and primates, but also livestock such as cattle, pigs, chickens, sheep, horses, dogs, mice, rats, and cats.

[0011] In another embodiment of the present invention, the method preferably involves culturing cells by an adhesive or floating method, but is not limited thereto.

[0012] In a preferred embodiment of the present invention, the method is preferably cultured for 3 days, but is not limited thereto.

[0013] According to one embodiment of the present invention, chicken muscle-derived fibroblasts can be proliferated by attachment culture. The characteristics of the proliferated cells were not different from those when conventional fetal bovine serum was used.

[0014] The Hanwoo serum isolated through this invention does not have the ethical issues associated with bovine fetal serum and enables the economical production of cultured meat, thereby contributing to the mass production and commercialization of cultured meat.

[0015] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by a skilled expert in the art to which this invention pertains. Effects of the invention

[0016] Through this invention, an economical culture medium for producing cultured meat can be produced by utilizing discarded slaughter blood from Korean beef cattle, thereby reducing the cost of cultured meat production. Furthermore, it can strengthen competitiveness in domestic and international markets and contribute to environmental protection by reducing greenhouse gas emissions and recycling livestock waste. This enables the establishment of a sustainable food supply system.

[0017] By utilizing slaughterhouse by-products, we can create added value for the livestock industry and establish a new industrial ecosystem through convergence with the bio-industry. This will enable us to lead the sustainable future food industry and contribute to establishing ourselves as a global leader in cultured meat technology. Brief explanation of the drawing

[0018] Figure 1 shows the process of collecting slaughterhouse blood and coagulating it in a container (a), separating the coagulated blood and serum through centrifugation (b), collecting only the serum separately (c), and filtering it before preparing the culture medium (d). Figure 2 shows the cell proliferation rate of Hanwoo serum (HS) using chicken muscle-derived fibroblasts (cMDF). Figure 3 shows the results of confirming whether chicken muscle-derived fibroblasts maintain their cell characteristics after being cultured in fetal bovine serum (FBS) and Hanwoo serum (HS). Figure 4 is a figure analyzing the characteristics of Hanwoo serum components by comparing fetal bovine serum (FBS), bovine serum (ABS), and Hanwoo serum (HS) through proteomic analysis. Specific details for implementing the invention

[0019] The present invention will be described in more detail below through non-limiting examples. However, the following examples are provided for the purpose of illustrating the present invention, and the scope of the present invention should not be interpreted as being limited by the following examples.

Claims

Claim 1 A method of coagulating blood within a container by dispensing the blood before coagulation occurs after collecting slaughter blood. Claim 2 A method of separating blood clots and serum through centrifugation of coagulated blood Claim 3 A method of passing separated serum through a filter to ensure safety and preparing a culture medium for meat production Claim 4 A method for culturing animal-derived cells using a culture medium for meat production and verifying the cell proliferation rate Claim 5 A method for conducting component analysis and identifying active ingredients through proteomic analysis using bovine fetal serum, bovine serum, and Hanwoo serum

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