Composition for improving vaginal mucosal health containing a DNA complex derived from salmonid fish

Abstract

The present invention relates to a composition for improving vaginal mucosal health comprising a DNA complex derived from salmonid fish, and more specifically, comprises a DNA complex derived from salmonid fish, hyaluronic acid, a natural moisture retainer, a pH buffer, and a solvent. The composition for improving vaginal mucosal health, comprising the above-mentioned ingredients, inhibits inflammation of the vaginal mucosa and promotes cell regeneration. It also exhibits the effect of improving vaginal mucosal health by minimizing mucosal irritation and providing a moisturizing effect through optimal osmotic pressure regulation and strong moisture retention.

Description

Technology Field

[0001] The present invention relates to a composition for improving vaginal mucosal health comprising a DNA complex derived from salmonid fish, and more specifically, to a composition for improving vaginal mucosal health comprising a DNA complex derived from salmonid fish that inhibits inflammation and promotes cell regeneration of the vaginal mucosa, and exhibits the effect of improving vaginal mucosal health by minimizing mucosal irritation and providing a moisturizing effect through optimal osmotic pressure regulation and strong moisture retention. Background Technology

[0003] DNA complexes are bioactive substances corresponding to the nucleic acid components of cells. Depending on their intended use and manufacturing form, they are referred to as Sodium DNA, polydeoxyribonucleotide (PDRN), polynucleotide (PN), or nucleic acid, and are known to contribute to the promotion of cell proliferation, tissue regeneration, wound healing, and the alleviation of inflammatory responses. Due to these characteristics, the scope of application for DNA complexes is gradually expanding in skin regeneration, tissue repair, and various medical and bio fields. In particular, PDRN and PN are highly valued as regenerative therapeutic materials, as numerous studies have reported their ability to promote the recovery of damaged tissues and induce cell activation.

[0004] However, in conventional technology, there was still room for improvement in terms of extraction efficiency, impurity removal, ensuring stability, and maintaining high purity during the process of extracting fish-derived nucleic acid materials; furthermore, there was a problem in that these manufacturing limitations could affect the quality uniformity of the final product and its biocompatibility.

[0005] Meanwhile, despite the fact that the female vaginal mucosa is a sensitive tissue prone to dryness, micro-damage, and inflammation caused by various factors such as changes in the external environment, hormonal fluctuations, aging, and microbial imbalances, existing vaginal washes, lubricants, or mucosal protective products have often remained limited to focusing primarily on temporary moisturization or antibacterial action. Consequently, functions that promote the fundamental recovery of damaged mucosal tissue or cell regeneration have not been sufficiently realized. Furthermore, despite the excellent biological activity of DNA-based regenerative materials, technologies capable of simultaneously satisfying formulation design, osmotic pressure regulation, pH stability maintenance, and mucosal irritation minimization for their appropriate application to the vaginal mucosal environment have not yet been fully established.

[0006] Furthermore, conventional products tend to be developed for a single purpose, such as specific functions like moisturizing or antibacterial action, which has limited their ability to comprehensively improve the complex problems of inflammation, dryness, tissue weakening, and functional decline occurring in the vaginal mucosa.

[0007] Therefore, there is a need to develop a composition for improving vaginal mucosal health that can comprehensively improve vaginal mucosal health by organically combining a DNA complex that promotes tissue regeneration, a moisture-retaining component, and a mucosal-friendly environmental control system to simultaneously ensure safety and functionality. Prior art literature

[0009] Korean Patent Registration No. 10-2296688 (Aug. 26, 2021) Korean Patent Registration No. 10-2588878 (October 10, 2023) The problem to be solved

[0010] The objective of the present invention is to provide a composition for improving vaginal mucosal health comprising a salmonid fish-derived DNA complex that inhibits inflammation of the vaginal mucosa and promotes cell regeneration, and improves vaginal mucosal health by minimizing mucosal irritation and providing a moisturizing effect through optimal osmotic pressure regulation and strong moisture retention. means of solving the problem

[0012] The objective of the present invention can be achieved by providing a composition for improving vaginal mucosal health characterized by comprising a DNA complex derived from salmonid fish, hyaluronic acid, a natural moisture retainer, a pH buffer, and a solvent.

[0013] According to a preferred feature of the present invention, the composition for improving vaginal mucosal health is characterized by comprising 0.1 to 3 weight% of a DNA complex derived from salmonid fish, 0.1 to 3 weight% of hyaluronic acid, 0.05 to 2 weight% of a natural moisture retainer, 0.01 to 0.5 weight% of a pH buffer, and the remainder of a solvent.

[0014] According to a preferred feature of the present invention, the salmonid-derived DNA complex is characterized by comprising one or more selected from the group consisting of polynucleotides, polydeoxyribonucleotides, and sodium DNA.

[0015] According to a preferred feature of the present invention, the natural moisture retainer is characterized by comprising one or more selected from the group consisting of mannitol, sorbitol, trehalose, xylitol, erythritol, glucose, fructose, glycerin, beta-glucan, panthenol, betaine, and ectoin.

[0016] According to a preferred feature of the present invention, the pH buffer comprises one or more selected from the group consisting of sodium phosphate, disodium phosphate, citric acid, sodium citrate, lactic acid, and sodium lactate.

[0017] According to a preferred feature of the present invention, the composition for improving vaginal mucosal health further comprises 0.1 to 5 weight percent of an additive, wherein the additive comprises one or more selected from the group consisting of Centella asiatica extract, allantoin, hydrolyzed collagen, and hydrolyzed elastin.

[0018] According to a preferred feature of the present invention, the composition for improving vaginal mucosal health is characterized by being applied in one formulation selected from the group consisting of gels, creams, suppositories, sprays, vaginal cleansers, sanitary pads, panty liners, and vaginal cleansing wipes. Effects of the invention

[0020] The composition for improving vaginal mucosal health comprising a DNA complex derived from salmonid fish according to the present invention inhibits inflammation of the vaginal mucosa and promotes cell regeneration, and exhibits excellent effects in improving vaginal mucosal health by minimizing mucosal irritation and providing a moisturizing effect through optimal osmotic pressure regulation and strong moisture retention. Specific details for implementing the invention

[0022] Hereinafter, preferred embodiments of the present invention and the physical properties of each component are described in detail. This description is intended to be sufficient for a person skilled in the art to easily practice the invention, and does not imply that the technical scope and concept of the present invention are limited thereby.

[0024] The composition for improving vaginal mucosal health according to the present invention comprises a DNA complex derived from salmonid fish, hyaluronic acid, a natural moisture retainer, a pH buffer, and a solvent, and preferably comprises 0.1 to 3 weight% of the DNA complex derived from salmonid fish, 0.1 to 3 weight% of hyaluronic acid, 0.05 to 2 weight% of the natural moisture retainer, 0.01 to 0.5 weight% of the pH buffer, and the remainder of the solvent.

[0026] The above salmonid fish-derived DNA complex is included in an amount of 0.1 to 3 weight% and preferably includes one or more selected from the group consisting of polynucleotide (PN), polydeoxyribonucleotide (PDRN), and sodium DNA. The salmonid fish-derived DNA complex composed of the above components not only exhibits an anti-inflammatory effect through the activation of intracellular adenosine A2A receptors, but also promotes fibroblast proliferation and collagen synthesis in damaged vaginal mucosa, thereby inducing the regeneration of mucosal tissue and restoring structural elasticity.

[0027] In addition, by being broken down in vivo to supply purine and pyrimidine bases, it plays a role in shortening the energy-intensive process of cell division and helping with rapid tissue repair.

[0028] Based on its high viscoelasticity, the aforementioned polynucleotide acts as a structural support for damaged vaginal mucosal tissue and plays a role in maintaining an optimal environment for tissue regeneration for an extended period.

[0029] In addition, the aforementioned polydeoxyribonucleotide is a low-molecular-weight DNA fraction of a specific size that rapidly binds to cell receptors to promote anti-inflammatory effects and neovascularization, and plays a role in improving the rate of initial tissue repair by inducing rapid cell proliferation.

[0030] In addition, the sodium DNA is in the form of a sodium salt of a nucleic acid component and has excellent biocompatibility. It serves as a basic active ingredient that ensures the water solubility and stability of the active ingredients in the formulation and rapidly supplies moisture and nutrition to the mucosal surface.

[0031] At this time, the above-mentioned DNA complex derived from salmonid fish can be prepared through a process of purifying the testis extract by adding the fish testis to brine, heating it, and then centrifuging it; degrading the purified testis extract using a technique such as enzymatic degradation using a proteolytic enzyme mixture or ultrasonic extraction using ultrasound irradiation, purifying it using centrifugation or chromatography, segmenting the purified testis extract, adjusting the pH of the segmented testis extract, immersing the pH-adjusted testis extract in an ethanol mixture and centrifuging it to obtain the DNA complex, and freeze-drying the obtained DNA complex.

[0032] To explain in more detail the preparation process of polydeoxyribonucleotide among the above DNA complex as an example, brine is prepared by mixing 5 kg of sodium chloride with 50 L of purified water; 10 kg of salmon testes are added to the prepared brine and heated at 95°C for 1 hour to prepare a salmon teste extract; after cooling to 4°C and centrifuging at 13,000 rpm for 15 minutes, the supernatant is recovered to obtain a purified salmon teste extract; 11 parts by weight of a proteolytic enzyme mixture (100 parts by weight of sodium dodecyl sulfate, 0.5 parts by weight of protease, and 0.25 parts by weight of sodium hydroxide) is mixed with 100 parts by weight of the obtained salmon teste extract and stirred at room temperature for 24 hours to remove impurities; the process of cooling to 4°C and centrifuging at 13,000 rpm for 15 minutes is repeated twice, and the supernatant is recovered to obtain purified salmon A testicular extract is obtained; 100 parts by weight of the obtained salmon testicular extract are mixed with 0.6 parts by weight of an enzyme mixture (100 parts by weight of protease and 20 parts by weight of amylase) and stirred at 45°C for 48 hours to fragment; sodium hydroxide is mixed with the fragmented salmon testicular extract to adjust the pH to 5.5, and after stirring at 4°C for 10 minutes, the mixture is filtered through a 0.45 µm filter (PVDF) and additionally mixed with sodium hydroxide to adjust the pH to 7.0; 100 parts by weight of the salmon testicular extract adjusted to pH 7.0 are immersed in 200 parts by weight of an ethanol mixture (isopropyl alcohol and ethanol mixed in a ratio of 3:7) for 24 hours and centrifuged at 4°C and a speed of 13,000 rpm to obtain fragmented polydeoxyribonucleotides; and the obtained polydeoxyribonucleotides It can be manufactured through a process of rapid freezing at -35℃, drying for 10 hours at a temperature of 30℃ and a vacuum of 5000 mTorr, drying for 52 hours at a temperature of 37.5℃ and a vacuum of 800 mTorr, and drying for 10 hours at a temperature of 30℃ and a vacuum of 800 mTorr.

[0033] If the content of the above-mentioned salmonid fish-derived DNA complex is less than 0.1% by weight, the cell regeneration and anti-inflammatory effects of the vaginal mucosa are negligible, making it difficult to expect substantial health improvement. If the content of the above-mentioned salmonid fish-derived DNA complex exceeds 3% by weight, the viscosity of the formulation increases excessively, which not only reduces the user experience but also increases mucosal irritation caused by high-concentration nucleic acid components.

[0035] In addition, the hyaluronic acid is included in an amount of 0.1 to 3 weight% and acts as a moisturizing and structural support that maximizes the protection and regeneration efficiency of the mucosal barrier by forming an immediate moisturizing film on the dried vaginal mucosa based on its strong moisture retention capacity that attracts moisture up to thousands of times its own weight, as well as creating an extracellular matrix (ECM) environment that helps the migration and proliferation of vaginal mucosal epithelial cells through interaction with salmonid fish-derived DNA complexes.

[0036] If the content of the hyaluronic acid is less than 0.1% by weight, the moisture retention capacity of the vaginal mucosal surface is insufficient, making it difficult to expect immediate moisturizing and lubricating effects. If the content of the hyaluronic acid exceeds 3% by weight, the viscosity of the formulation becomes excessively high, which not only reduces spreadability but also causes a counterproductive effect of depleting moisture within the mucosa due to an osmotic pressure imbalance with the mucosa, or may cause a foreign body sensation.

[0038] The above natural moisture retainer is included in an amount of 0.05 to 2 weight% and comprises one or more selected from the group consisting of mannitol, sorbitol, trehalose, xylitol, erythritol, glucose, fructose, glycerin, beta-glucan, panthenol, betaine, and ectoin. The natural moisture retainer composed of the above ingredients acts as an osmolyte that minimizes mucosal irritation by finely regulating the effective osmotic concentration of the vaginal mucosa, thereby exhibiting a mucosal protective effect through osmotic pressure regulation. It also plays a role in improving the structural stability of the above salmonid fish-derived DNA complex and the above hyaluronic acid, protecting the physiological activity of the active ingredient from changes in the external environment, and further improving the moisture retention time within the mucosa.

[0039] The aforementioned mannitol prevents the breakdown of DNA complexes through its powerful antioxidant function and minimizes mucosal irritation by optimizing the osmotic pressure of the mucosal surface to prevent moisture from leaking out.

[0040] In addition, the sorbitol forms a thin moisture film on the mucous membrane through a molecular structure that attracts moisture from the air, preventing moisture within the formulation from evaporating and thereby improving the longevity of moisturization.

[0041] In addition, the trehalose mentioned above has excellent binding ability to firmly fix water molecules, so it inhibits the denaturation of the cell membrane even when dry and forms a moisture protective film to maintain the viability of mucosal cells.

[0042] In addition, the above xylitol, due to its structural characteristics of having multiple hydroxyl groups, keeps the mucous membrane moist and hinders the attachment of harmful bacteria while providing a cooling sensation, thereby helping to alleviate itching.

[0043] In addition, the erythritol above supplies moisture to the mucous membrane with a skin-friendly hydrophilic structure and provides a cooling moisturizing effect that soothes the heat sensation of the mucous membrane caused by inflammation using heat of vaporization.

[0044] In addition, the glucose and fructose mentioned above supply energy sources for cell regeneration, and at the same time, form hydrogen bonds with water through hydroxyl groups (-OH) to improve the basic moisture retention of the mucous membrane.

[0045] In addition, the glycerin mentioned above is a natural moisturizing agent that delivers moisture to the deep layers of the mucous membrane and has excellent ability to absorb moisture from the air, acting as a lubricating film that protects the mucous membrane from physical friction.

[0046] In addition, the aforementioned beta-glucan is a high-molecular-weight polysaccharide with superior moisture retention capacity compared to hyaluronic acid, and it plays a role in maintaining moisture for a long time by forming an elastic moisture layer on the mucosal surface.

[0047] In addition, the aforementioned panthenol is a water-soluble moisturizer with excellent penetration into the skin and mucous membranes. It plays a role in retaining moisture within the mucous membranes and repairing damaged barriers to fundamentally prevent moisture loss.

[0048] In addition, the above-mentioned betaine acts as an osmolite that regulates the water balance inside and outside the cell, thereby preventing mucosal cells from losing water and shrinking.

[0049] In addition, the above-mentioned ectoin forms a strong complex (Ectoin-Water Complex) with water molecules to coat the mucous membrane, protecting the mucous membrane from external irritants while simultaneously creating a moisture-retaining environment.

[0050] If the content of the above natural moisture retainer is less than 0.05 weight%, it is difficult to maintain the appropriate osmotic pressure of the vaginal mucosa, and a problem arises in which the moisturizing effect is rapidly reduced. If the content of the above natural moisture retainer exceeds 2 weight%, the adhesiveness of the formulation becomes excessive, which may cause stickiness during use or disrupt the moisture balance of mucosal epithelial cells, thereby causing irritation.

[0052] The above pH buffer is included in an amount of 0.01 to 0.5 weight% and comprises one or more selected from the group consisting of sodium phosphate, disodium phosphate, citric acid, sodium citrate, lactic acid, and sodium lactate. The pH buffer composed of the above components controls and maintains the pH of the composition for improving vaginal mucosal health produced through the present invention within a range of 5.0 to 7.0, thereby minimizing chemical irritation applied to the vaginal mucosa, and ensures the structural stability of the salmonid fish-derived DNA complex and hyaluronic acid, thereby maximizing penetration into the mucosa and regeneration efficiency without loss of active ingredients.

[0053] If the content of the above pH buffer is less than 0.01 weight%, it is difficult to maintain an appropriate pH, which may result in deterioration of the ingredients or mucosal irritation, and if the content of the above pH buffer exceeds 0.5 weight%, the stability of the formulation may be reduced due to excessive ion concentration or it may affect the natural acidity regulation mechanism of the mucous membrane.

[0055] The above solvent includes purified water or physiological saline solution. The solvent containing the above components serves as a medium for uniformly dissolving and dispersing key active ingredients, such as DNA complexes, hyaluronic acid, and moisture retainers. In particular, when physiological saline solution is used, it forms an ionic strength similar to body fluids, thereby preventing cell irritation caused by osmotic pressure differences upon application to the vaginal mucosa and enhancing the bioavailability of the components, which helps ensure the safe delivery of the composition.

[0057] In addition, the composition for improving vaginal mucosal health according to the present invention may further include 0.1 to 5 weight percent of an additive, and it is preferable that the additive includes one or more selected from the group consisting of Centella asiatica extract, allantoin, hydrolyzed collagen, and hydrolyzed elastin.

[0058] The additive composed of the above ingredients provides powerful soothing, cell proliferation-promoting, and tissue elasticity-reinforcing functions, complementing the regenerative function of DNA complexes and accelerating the recovery speed of damaged mucosa. Furthermore, it exhibits the effect of comprehensively improving the flexibility and structural strength of aged or atrophied vaginal mucosa.

[0059] The above Centella asiatica extract contains madecassoside, which accelerates the regeneration of damaged mucous membranes and provides a powerful soothing effect.

[0060] In addition, the aforementioned allantoin directly aids in cell proliferation and protects irritated mucous membranes, and plays a role in facilitating the penetration of active ingredients through keratolytic action.

[0061] In addition, the above hydrolyzed collagen is a collagen that has been hydrolyzed to increase absorption rate, and plays a role in strengthening the elasticity of mucosal tissue and assisting in moisture binding.

[0062] In addition, the hydrolyzed elastin, together with the hydrolyzed collagen, plays a role in improving the health of aged or atrophied mucous membranes by restoring flexibility and elasticity to mucous membrane tissues.

[0063] If the content of the above additive is less than 0.1 weight%, the above effect is negligible, and if the content of the above additive exceeds 5 weight%, it may impair the formulation stability with the main active ingredient, DNA complex or hyaluronic acid, and may instead cause allergic or hypersensitive reactions in the sensitive vaginal mucosa.

[0065] A composition for improving vaginal mucosal health composed of the above ingredients can be prepared by dissolving a DNA complex derived from salmonid fish in a solvent, mixing and stirring hyaluronic acid to homogenize it, mixing a natural moisture retainer to ensure osmotic pressure and moisturizing stability, and mixing a pH buffer to adjust the pH to 5.0 to 7.0. It is preferable to perform high-pressure steam sterilization using an autoclave at a temperature of 115 to 125°C for 10 to 20 minutes on the prepared composition for improving vaginal mucosal health.

[0066] In addition, the manufactured composition for improving vaginal mucosal health can be applied as one formulation selected from the group consisting of gels, creams, suppositories, sprays, vaginal washes, sanitary pads, panty liners, and vaginal cleansing wipes.

[0068] Hereinafter, the method for preparing a composition for improving vaginal mucosal health according to the present invention and the physical properties of the composition for improving vaginal mucosal health prepared by said method will be explained with reference to examples.

[0070] <Preparation Example 1> Preparation of Additives

[0071] An additive was prepared by mixing Centella asiatica extract, allantoin, hydrolyzed collagen, and hydrolyzed elastin in a weight ratio of 1:0.5:1:0.3.

[0073] <Example 1>

[0074] A composition for improving vaginal mucosal health was prepared by mixing 1.5% by weight of a DNA complex derived from salmonid fish (a mixture of polynucleotide and polydeoxyribonucleotide in a 1:1 weight ratio), 1.5% by weight of hyaluronic acid, 1% by weight of a natural moisture retainer (mannitol), 0.3% by weight of a pH buffer (sodium phosphate), and the remainder of purified water, and sterilizing it for 15 minutes with steam at a temperature of 121°C using an autoclave.

[0076] <Example 2>

[0077] A composition for improving vaginal mucosal health was prepared by proceeding in the same manner as in Example 1 above, but mixing 0.1 wt% of a salmonid-derived DNA complex, 0.1 wt% of hyaluronic acid, 0.05 wt% of a natural moisture retainer, 0.01 wt% of a pH buffer, and the remaining amount of solvent.

[0079] <Example 3>

[0080] A composition for improving vaginal mucosal health was prepared by mixing 3% by weight of a salmonid-derived DNA complex, 3% by weight of hyaluronic acid, 2% by weight of a natural moisture retainer, 0.5% by weight of a pH buffer, and the remaining amount of solvent, in the same manner as in Example 1 above.

[0082] <Example 4>

[0083] A composition for improving vaginal mucosal health was prepared by proceeding in the same manner as in Example 1 above, but by further mixing 2.5% by weight of the additive prepared through Preparation Example 1 above.

[0085] <Example 5>

[0086] A composition for improving vaginal mucosal health was prepared by proceeding in the same manner as in Example 1 above, but by further mixing 0.1% by weight of the additive prepared through Preparation Example 1 above.

[0088] <Example 6>

[0089] A composition for improving vaginal mucosal health was prepared by proceeding in the same manner as in Example 1 above, but by further mixing 5% by weight of the additive prepared through Preparation Example 1 above.

[0091] <Comparative Example 1>

[0092] A composition for improving vaginal mucosal health was prepared by mixing 1.5% by weight of a DNA complex derived from salmonid fish (a mixture of polynucleotide and polydeoxyribonucleotide in a 1:1 weight ratio) and the remainder of purified water, and sterilizing it for 15 minutes with steam at a temperature of 121°C using an autoclave.

[0094] <Comparative Example 2>

[0095] A composition for improving vaginal mucosal health was prepared by proceeding in the same manner as in Example 1 above, but mixing 0.05 wt% of a salmonid fish-derived DNA complex, 0.05 wt% of hyaluronic acid, 0.025 wt% of a natural moisture retainer, 0.005 wt% of a pH buffer, and the remaining amount of solvent.

[0097] <Comparative Example 3>

[0098] A composition for improving vaginal mucosal health was prepared by mixing 6% by weight of a salmonid-derived DNA complex, 6% by weight of hyaluronic acid, 4% by weight of a natural moisture retainer, 1% by weight of a pH buffer, and the remaining amount of solvent, in the same manner as in Example 1 above.

[0100] <Experimental Example 1> Measurement of the regenerative effect of vaginal mucosal epithelial cells

[0101] The regenerative effect of the vaginal mucosal epithelial cells of the compositions for improving vaginal mucosal health prepared through the above Examples 1 to 6 and Comparative Examples 1 to 3 was measured and is shown in Table 1 below.

[0102] {However, the regenerative effect of vaginal mucosal epithelial cells was tested using the method according to ISO 10993-5, and human vaginal mucosal epithelial cells (VK2 / E6E7) were cultured, treated with the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 and Comparative Examples 1 to 3, and the cell proliferation rate was measured by an MTT assay after 72 hours.

[0103] Hyaluronic acid was used as the control group.

[0104]

[0105]

[0106] As shown in Table 1 above, it can be seen that the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 of the present invention exhibit a superior regenerative effect on vaginal mucosal epithelial cells compared to Comparative Example 2, and in particular, it can be seen that the compositions for improving vaginal mucosal health prepared through Examples 4 to 6 exhibit an even more superior regenerative effect on vaginal mucosal epithelial cells.

[0108] <Experimental Example 2> Measurement of Anti-inflammatory Effect

[0109] The anti-inflammatory effect of the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 and Comparative Examples 1 to 3 above was measured and is shown in Table 2 below.

[0110] {However, for the anti-inflammatory effect, an inflammatory response was induced in macrophages by treating them with LPS (Lipopolysaccharide, 1 μg / mL), an inflammation-inducing substance, and then the compositions of Examples 1 to 6 and Comparative Examples 1 to 3 were applied, respectively. Subsequently, the expression level of TNF-α, a major inflammation-inducing cytokine, in the supernatant cultured for 24 hours was measured using an ELISA (Enzyme-Linked Immunosorbent Assay) kit, and the method of calculating the rate of expression reduction compared to the control group (LPS-alone treatment group) was used.}

[0111] Table 2

[0112]

[0113] As shown in Table 2 above, it can be seen that the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 of the present invention exhibit superior anti-inflammatory effects compared to the compositions for improving vaginal mucosal health prepared through Comparative Examples 1 to 3, and in particular, it can be seen that the anti-inflammatory effects of the compositions for improving vaginal mucosal health prepared through Examples 4 to 6 are even more superior.

[0115] <Experimental Example 3> Measurement of Cytotoxicity

[0116] The cytotoxicity of the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 and Comparative Examples 1 to 3 above was measured and is shown in Table 4 below.

[0117] However, cytotoxicity was tested using the method according to ISO 10993-5. Standardized human vaginal mucosal epithelial cells (VK2 / E6E7) were cultured as a single layer in a 96-well plate, treated with compositions diluted at stepwise concentrations, and cultured for 72 hours at 37°C in a 5% CO2 incubator. Subsequently, MTT reagent was added to dissolve the purple formazan crystals produced by the mitochondrial enzymes of the surviving cells, and the absorbance was measured.

[0118] In addition, the cytotoxicity grade was determined based on the evaluation table shown in Table 3 below.

[0119] Table 3

[0120]

[0121] Table 4

[0122]

[0123] As shown in Table 4 above, it can be seen that the compositions for improving vaginal mucosal health prepared through Examples 1 to 6 of the present invention exhibit lower cytotoxicity compared to the compositions for improving vaginal mucosal health prepared through Comparative Example 1 and Comparative Example 3.

[0125] Accordingly, the composition for improving vaginal mucosal health comprising a salmonid-derived DNA complex according to the present invention inhibits inflammation of the vaginal mucosa and promotes cell regeneration, and improves vaginal mucosal health by minimizing mucosal irritation and providing a moisturizing effect through optimal osmotic pressure regulation and strong moisture retention.

Claims

Claim 1 A composition for improving vaginal mucosal health comprising 0.1 to 3 weight% of a DNA complex derived from salmonid fish, 0.1 to 3 weight% of hyaluronic acid, 0.05 to 2 weight% of a natural moisture retainer, 0.01 to 0.5 weight% of a pH buffer, and the remainder of a solvent, wherein the DNA complex derived from salmonid fish is composed of a mixture of polynucleotide and polydeoxyribonucleotide in a 1:1 weight ratio, the natural moisture retainer is composed of mannitol, and the pH buffer is composed of sodium phosphate. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 A composition for improving vaginal mucosal health according to claim 1, wherein the composition for improving vaginal mucosal health further comprises 0.1 to 5 weight% of an additive, and the additive comprises one or more selected from the group consisting of Centella asiatica extract, allantoin, hydrolyzed collagen, and hydrolyzed elastin. Claim 7 A composition for improving vaginal mucosal health according to claim 1, characterized in that the composition for improving vaginal mucosal health is applied as a formulation selected from the group consisting of gel, cream, suppository, spray, vaginal cleanser, sanitary pad, panty liner, and vaginal cleansing wipe.

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