Composition for improving skin condition comprising red houttuynia cordata, an extract of houttuynia cordata var. purpurea

Abstract

The present invention relates to a cosmetic composition for improving skin condition comprising a complex of extracts from the flowers, leaves, and stems of Houttuynia cordata. The complex comprises extracts from the flowers, leaves, and stems of Houttuynia cordata mixed in a specific ratio and exhibits excellent antioxidant, pigmentation and wrinkle improvement, and skin elasticity improvement effects, making it useful for improving skin condition.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition for improving skin condition comprising a complex of extracts from the flowers, leaves, and stems of Houttuynia cordata. Background Technology

[0003] In modern society, skin aging and damage caused by external stimuli such as ultraviolet rays, fine dust, stress, and environmental pollution are recognized as one of the biggest cosmetic problems. These factors increase the production of reactive oxygen species (ROS), inducing oxidative stress. This leads to skin cell damage, collagen degradation, and excessive melanin production, thereby accelerating aging phenomena such as wrinkle formation, loss of elasticity, and pigmentation.

[0004] Specifically, melanin, which determines human skin color, is produced in melanocytes. More specifically, melanin is a dark brown pigment formed through a polymerization and oxidation reaction mediated by enzymes such as tyrosinase present in melanocytes, using tyrosine—an amino acid naturally present in the body—as a substrate. The melanin thus formed then travels through the dendritic processes of melanocytes to epidermal cells called keratinocytes. Once transported to keratinocytes, the melanin can be removed by shedding along with the skin when the keratinocytes detach from the epidermis. However, since the body lacks enzymes to break down melanin, once formed, it is not broken down internally. Therefore, inhibiting the production of melanin is the key to achieving lighter skin.

[0005] Skin wrinkles and loss of elasticity are caused by a decrease in collagen and elastin. Collagen is a protein that supports the structure of the dermis and decreases every year starting from the age of 20. Elastin is a protein that maintains skin elasticity and is almost impossible to regenerate after damage. Therefore, to improve skin wrinkles and loss of elasticity, the breakdown of elastin must be prevented.

[0006] To address these skin problems, research and development are actively underway on natural functional materials effective for skin antioxidant, whitening, and wrinkle improvement. In particular, plant-derived natural extracts are attracting attention in the fields of cosmetics and quasi-drugs because they have the advantage of being safer than synthetic chemicals and possessing various biological activities simultaneously. Prior art literature

[0008] 1. Antioxidant and anti-aging activities of Houttuynia cordata extracts according to extraction solvent, J Invest Cosmetol Vol. 15, No. 2, pp. 147-157 (2019) 2. Houttuynia cordata Thunb inhibits the production of pro-inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC-1 human mast cells, MOLECULAR MEDICINE REPORTS 2013, 8, 731-736. The problem to be solved

[0009] Under the circumstances described above, the inventors conducted research to discover a natural plant-derived extract capable of effectively improving skin condition. They confirmed that an extract derived from *Houttuynia cordata* has excellent antioxidant effects, and that in particular, mixing the flower, leaf, and stem extracts of *Houttuynia cordata* in a specific ratio significantly improves skin condition, thereby completing the present invention.

[0010] Therefore, the objective of the present invention is to [pronounced] red-patterned Houttuynia cordata ( Houttuynia cordata var. purpureaThe present invention provides a cosmetic composition for improving skin condition that includes an extract of ) as an active ingredient. means of solving the problem

[0012] To achieve the above objective, one aspect of the present invention is red-patterned Houttuynia cordata ( Houttuynia cordata var. purpurea A cosmetic composition for improving skin condition is provided, comprising an extract of ) as an active ingredient.

[0013] Houttuynia cordata Houttuynia cordata ) is Sambeakchoga ( Saururaceae It is a perennial herbaceous plant belonging to the genus Houttuynia cordata that has long been used for medicinal and edible purposes in East Asia, and is traditionally known for its detoxifying, anti-inflammatory, antiviral, and antibacterial effects. According to existing studies, the leaves and stems of Houttuynia cordata are rich in quercetin, houttuynin, polyphenols, and flavonoids, which provide antioxidant and anti-inflammatory effects (Non-patent Literature 1 and 2). Due to these effects, Houttuynia cordata extract is attracting attention as a natural cosmetic ingredient that provides skin trouble relief, inflammation suppression, sebum control, antioxidant, and anti-aging effects.

[0014] In addition to the commonly known green-leaved Houttuynia cordata, there exist varieties with red or purple patterns along the leaf veins, which are called "red-patterned Houttuynia cordata ( Houttuynia cordata var. purpurea It is called )".

[0015] To date, most prior research has focused on extracts limited to the leaves or stems of Houttuynia cordata, and studies on the bioactive components and efficacy of the flowers are very limited. In particular, the skin-improving effects of substances derived from Houttuynia cordata are not yet known.

[0016] Accordingly, the inventors studied the flower, leaf, and stem extracts of Houttuynia cordata and confirmed that while each extract has excellent antioxidant activity, its effect is enhanced when used in combination, thereby completing the present invention. In a specific embodiment of the present invention, the extract with the enhanced effect is described as "Red Houttuynia cordata."

[0017] In the present invention, "improvement of skin condition" refers to reducing the degree of symptoms related to the deterioration of the skin condition compared to before using the cosmetic composition, and the skin condition may be a parameter related to skin elasticity, skin wrinkles, skin texture, skin tone, skin brightness, skin regeneration, and skin whitening. Accordingly, the improvement of skin condition may mean improvement of skin elasticity, reduction of skin wrinkles, improvement of skin texture, improvement of skin tone, improvement of skin brightness, promotion of skin regeneration, and skin whitening.

[0018] As used in this specification, the term "pigmentation" refers to the phenomenon in which the skin turns dark due to the excessive production of melanin, the pigment that determines the color of human skin, hair, and eyes, caused by skin damage, exposure to ultraviolet rays, friction, inflammation, skin diseases, etc.

[0019] As used herein, the term "skin wrinkles" refers to fine lines formed due to skin aging, which can be caused by skin aging, genetic factors, a decrease in collagen and elastin present in the dermis, and external environmental factors. Skin aging may result from glycation reactions, habitual sleeping postures, loss of body mass, sun damage, or, temporarily, prolonged immersion in water. Skin aging is accelerated by habitual facial expressions, aging, sun damage, smoking, nutritional deficiencies, and various other factors. Accordingly, as used herein, the term "skin wrinkle improvement" means inhibiting or hindering the formation of wrinkles on the skin, or alleviating wrinkles that have already formed.

[0020] Meanwhile, the term "skin tone" as used in this specification refers to the state of the degree of intensity of skin color, and "skin tone improvement" refers to making an uneven skin tone uniform. "Skin whitening" and "skin brightness improvement" include increasing the brightness of skin that has decreased due to an excess of pigments such as melanin, or maintaining the brightness of the skin at a certain level.

[0021] Furthermore, "skin regeneration" refers to the recovery of skin tissue damaged by external and internal causes. The aforementioned external causes include ultraviolet rays, external pollutants, wounds, and trauma, while the aforementioned internal causes include diseases and stress. "Skin moisturization" refers to inhibiting or suppressing the decrease in skin moisture or increasing the skin's moisture content to smooth the skin surface and impart a radiant glow.

[0022] As used in this specification, the term "prevention" means taking precautions in advance against anticipated deterioration, and in the present invention, prevention means taking precautions in advance to prevent oxidation, pigmentation, and wrinkles from occurring.

[0023] The above oxidation refers to oxidative stress, and the term "oxidative stress" in this invention refers to a state in which cells are damaged because the amount of free radicals generated exceeds the removal capacity of antioxidants.

[0024] The term "extract" as used in the present invention refers to a preparation obtained by squeezing an extraction target with a suitable extractant and concentrating or filtering the extractant, and is not limited thereto, but may be an extract obtained by extraction treatment, a diluted or concentrated extract, a dried product obtained by drying an extract, or a crude purified product or purified product thereof.

[0025] The flower, leaf, and stem extracts of the above-mentioned red-patterned Houttuynia cordata can be prepared using general extraction, separation, and purification methods known in the ordinary art. The extraction methods may include, but are not limited to, hot water extraction, hot water extraction, cold maceration extraction, reflux cooling extraction, or ultrasonic extraction.

[0026] The above ultrasonic extraction is a method of extracting useful components from various natural materials at room temperature using physical force rather than chemical force by using a vibration element with a vibration frequency of more than 20,000 times per second. Unlike solvent extraction, vegetable oil extraction, or steam distillation extraction, it has the advantage of being able to extract active ingredients contained in natural materials at high concentrations at room temperature without destroying them.

[0027] As a specific example, the ultrasonic extraction can be performed on dried red-spotted Houttuynia cordata flowers, leaves, and stems using a 60% to 80% ethanol solution at room temperature for 24 to 72 hours. Preferably, it is performed using a 65% to 75% ethanol solution at room temperature for 36 to 60 hours, and more preferably, using a 70% ethanol solution at room temperature for 48 hours. If the lower limit of the above values ​​is exceeded, the active ingredients of the present invention contained in the red-spotted Houttuynia cordata flowers, leaves, and stems are not sufficiently extracted, and if the upper limit is exceeded, there is no significant difference in the amount of active ingredients extracted, impurities other than the active ingredients are extracted, and the efficiency of the process decreases.

[0028] As another specific example, the hot water extraction is carried out by adding purified water to dried red-patterned Houttuynia cordata flowers, leaves, and stems and reacting them at a high temperature, preferably 100 to 150°C, more preferably 100 to 130°C, more preferably 110 to 130°C, more preferably 121°C for 20 minutes to 1 hour, preferably 20 minutes to 50 minutes, more preferably 20 minutes to 40 minutes, and more preferably 30 minutes.

[0029] The red-patterned Houttuynia cordata flowers, leaves, and stems obtained above may be concentrated under reduced pressure using a vacuum vacuum concentrator or a vacuum rotary evaporator, though not limited thereto. Additionally, after concentration under reduced pressure, they may be dried through freeze-drying, vacuum drying, vacuum drying, boiling drying, or spray drying, though not limited thereto.

[0030] In the present invention, the extraction solvent may be selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, dichloromethane, acetone, and mixed solvents thereof, preferably ethanol, more preferably 40% to 80% ethanol, and most preferably 70% ethanol. Ethanol can easily extract intermediate polar components of the flavonoid series, which are active ingredients of Houttuynia cordata.

[0031] In one embodiment of the present invention, 10 times the amount of a 70% ethanol solution was added to 50 g of dried red-patterned Houttuynia cordata flowers, leaves, or stems, and an extract was prepared by refluxing for 3 hours, followed by filtration, concentration, and freeze-drying (Preparation Example 1).

[0032] In the present invention, the extract of Houttuynia cordata refers to an extract of the flower, leaf, and stem of Houttuynia cordata, respectively, or a complex of the three extracts mixed together. The red Houttuynia cordata described above is a mixture of the flower, leaf, and stem extracts of Houttuynia cordata, specifically a complex in which the three extracts are mixed in a weight ratio of 1 to 3: 1 to 3: 1 to 3. More preferably, the red Houttuynia cordata may be a mixture of the flower, leaf, and stem extracts of Houttuynia cordata in a weight ratio of 3: 2: 1.

[0033] In the present invention, the components included in the "cosmetic composition" may include, as active ingredients, components commonly used in cosmetic compositions in addition to said active ingredients, such as, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers. The cosmetic composition of the present invention may be prepared in any formulation commonly manufactured in the art, for example, as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, and spray, but is not limited thereto. More specifically, it may be prepared in the form of a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

[0034] In the case where the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0035] When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0036] In the case where the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanthese may be used as carrier components.

[0037] In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane / butane, or dimethyl ether.

[0038] In the case where the formulation of the present invention is a cleansing agent containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0039] When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation not containing a surfactant, it may be wiped off, peeled off, or washed off with water after application to the skin. As specific examples, the soap is a liquid soap, powder soap, solid soap, and oil soap; the cleansing formulation containing a surfactant is a cleansing foam, cleansing water, cleansing towel, and cleansing pack; and the cleansing formulation not containing a surfactant is a cleansing cream, cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

[0040] The content of red houttuynia cordata included in the above composition is not particularly limited thereto, but may be included in an amount of 0.00001 to 100 weight%, preferably 0.0001 to 15 weight%, more preferably 0.001 to 15 weight%, and even more preferably 0.001 to 10 weight% with respect to the total weight of the composition.

[0042] Another aspect of the present invention provides a pharmaceutical composition for skin antioxidant, skin whitening, and skin wrinkle improvement comprising an extract of red-patterned Houttuynia cordata as an active ingredient.

[0043] According to one embodiment of the present invention, the flower, leaf, and stem extracts of red-patterned Houttuynia cordata, and the red Houttuynia cordata mixed with said extracts, can prevent skin aging by inhibiting skin oxidative stress, and exhibit pigmentation inhibition and whitening effects by inhibiting the activity of tyrosinase, an enzyme related to pigmentation, and can improve skin elasticity by inhibiting the activity of elastase, an enzyme that degrades elastin, which plays an important role in maintaining elasticity, so they can be usefully used for skin antioxidant, pigmentation, and wrinkle prevention treatments.

[0044] The term "pharmaceutical composition" in the present invention refers to a composition prepared for the purpose of preventing or treating a disease, and may be formulated into various forms according to conventional methods. For example, it may be formulated into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, or formulated into parenteral formulations using diluents or excipients such as lubricants, humectants, flavoring agents, emulsifiers, suspending agents, preservatives, and surfactants. Additionally, it may be formulated into external preparations, suppositories, topical skin preparations, and sterile injectable solutions. As one specific example, the composition of the present invention may be used in an oral administration manner as an oral formulation. As another specific example, the composition of the present invention may be used by methods such as direct application or scattering to the scalp area where hair loss generally occurs.

[0045] In addition, depending on each formulation, it may be manufactured by additionally including pharmaceutically acceptable carriers, such as buffers, analgesics, solubilizers, isotonics, stabilizers, bases, etc., which are known in the art.

[0046] The term "pharmaceuticalally acceptable carrier" as used in the present invention may refer to a carrier or diluent that does not irritate living organisms and does not impair the biological activity and properties of the injected active ingredient. The types of carriers usable in the present invention are not particularly limited, and any carrier that is commonly used and pharmaceutically acceptable in the relevant technical field may be used. Non-limiting examples of said carriers include saline solution, sterile water, Ringer's solution, buffered saline solution, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, etc. These may be used alone or in a mixture of two or more. The said carrier may be a non-naturally occurring carrier.

[0047] The composition of the present invention may be administered in a pharmaceutically effective amount. The pharmaceutically effective amount refers to an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment and that does not cause side effects. The effective dose level may be determined based on factors including the patient's health condition, type and severity of the disease, drug activity, sensitivity to the drug, method of administration, time of administration, route of administration and elimination rate, duration of treatment, drugs used in combination or concurrently, and other factors well known in the medical field. Specifically, the dose is generally 0.01 mg to 5000 mg per day per 1 kg of body weight of the subject being administered, and may be divided into one or several doses per day at regular intervals at the discretion of a physician or pharmacist, but is not limited thereto.

[0048] In addition, the pharmaceutical composition of the present invention may be used alone, in combination with other pharmaceutically active compounds exhibiting antioxidant, whitening, and anti-wrinkle effects, or in a suitable combination.

[0049] The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. It may also be administered as a single or multiple doses. It is important to administer an amount that obtains maximum effect with a minimum amount without causing side effects, taking all of the above factors into consideration, and this can be easily determined by a person skilled in the art.

[0050] For example, but not limited to, in addition to the pharmaceutical composition containing red Houttuynia cordata of the present invention as an active ingredient, conventional whitening functional ingredients such as niacinamide and arbutin, or wrinkle improvement functional ingredients such as retinol and retinyl palmitate, may be selected and used additionally or in combination.

[0051] The method of administration of the pharmaceutical composition according to the present invention is not particularly limited and may follow methods commonly used in the relevant technical field. As a non-limiting example of the method of administration, the composition may be administered orally or parenterally. Specifically, the composition of the present invention may be used by methods such as direct application or scattering to the scalp area where hair loss generally occurs.

[0052] The pharmaceutical composition according to the present invention can be produced in various formulations depending on the intended method of administration.

[0054] Another aspect of the present invention provides an antioxidant, whitening, and anti-wrinkle topical skin composition comprising an extract of red-patterned Houttuynia cordata as an active ingredient.

[0055] The topical skin composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. This may be provided in any formulation suitable for topical application, for example, in the form of a solution, gel, solid, paste anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule, or ionic (liposome) and non-ionic vesicular dispersants, or in the form of a cream, skin toner, lotion, powder, ointment, spray, pack, or concealer stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

[0056] In addition, the topical skin composition according to the present invention may contain adjuvants commonly used in the field of cosmetics or dermatology, such as fatty substances, organic solvents, solvents, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion chelating agents, chelating agents, preservatives, vitamins, blockers, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, or any other ingredients commonly used in cosmetics. The adjuvants are introduced in amounts commonly used in the field of cosmetics or dermatology.

[0057] The content of red houttuynia cordata included in the above composition is not particularly limited thereto, but may be included in an amount of 0.00001 to 100 weight%, preferably 0.0001 to 15 weight%, more preferably 0.001 to 15 weight%, and even more preferably 0.001 to 10 weight% with respect to the total weight of the composition.

[0059] In another aspect, the present invention provides a functional food composition for antioxidant, whitening, and anti-wrinkle purposes comprising red Houttuynia cordata as an active ingredient.

[0060] The term "functional food" in the present invention refers to a food manufactured and processed using raw materials or ingredients having functional properties useful to the human body as defined in Article 6727 of the Health Functional Foods Act, and "functionality" means consuming for the purpose of obtaining useful effects for health purposes, such as regulating nutrients or physiological actions on the structure and function of the human body.

[0061] In addition, the term "health functional food" in the present invention refers to a food manufactured or processed by methods such as extraction, concentration, purification, or mixing, using a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplementation, and refers to a food designed and processed to sufficiently exert biological regulatory functions on the body, such as biological defense, regulation of biological rhythms, and prevention and recovery of disease, through said ingredient, and said composition for health food can perform functions related to the prevention of disease and recovery of disease.

[0062] When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweeteners, flavorings, or natural carbohydrates as additional ingredients, as in conventional beverages. In addition to the above, the health functional food composition of the present invention may contain various nutritional supplements, vitamins, electrolytes, flavorings, colorings, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Furthermore, it may contain fruit pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages.

[0063] The content of red houttuynia cordata included in the above composition is not particularly limited thereto, but may be included in an amount of 0.00001 to 100 weight%, preferably 0.0001 to 15 weight%, more preferably 0.001 to 15 weight%, and even more preferably 0.001 to 10 weight% with respect to the total weight of the composition.

[0065] Another aspect of the present invention provides a cosmetic method for improving the skin of a mammal, comprising the step of applying the aforementioned cosmetic composition to the skin of a mammal.

[0066] In the present invention, the mammal may be a human, dog, cat, rodent, horse, cow, monkey, or pig, but is not limited thereto.

[0067] In the present invention, the step of applying a cosmetic composition for improving skin condition to the skin of a mammal specifically comprises: (a) a step of directly applying the cosmetic composition for improving skin condition to the skin of a mammal; (b) a step of contacting or attaching a patch, mask pack, or mask sheet to which the cosmetic composition for improving skin condition has been applied or deposited to the skin of a mammal; or a step of sequentially performing (a) and (b).

[0068] The above-described composition containing red Houttuynia cordata of the present invention is made from natural medicinal plants, so it can be used safely as it may have fewer side effects compared to general synthetic compounds when used as a pharmaceutical composition, functional food composition, or cosmetic composition. Effects of the invention

[0070] Red Houttuynia cordata, which is a complex of flower, leaf, and stem extracts of Houttuynia cordata with red spots mixed in a specific ratio and is an active ingredient of the cosmetic composition of the present invention, can prevent skin aging by suppressing oxidative stress, exhibits pigmentation inhibition and whitening effects by inhibiting the activity of tyrosinase, an enzyme related to pigmentation, and can improve skin elasticity by inhibiting the activity of elastase, an enzyme that breaks down elastin, which plays an important role in maintaining elasticity; thus, it can be usefully used for preventing skin aging, preventing pigmentation and wrinkles, and improving skin elasticity. Brief explanation of the drawing

[0072] Figure 1 shows the results of evaluating the free radical scavenging activity of a complex of flower, leaf, and stem extracts of red-patterned Houttuynia cordata mixed in various ratios. Figure 2 shows the results of evaluating the free radical scavenging activity at different concentrations of a complex (Red Houttuynia cordata) in which flower, leaf, and stem extracts of red-patterned Houttuynia cordata are mixed in specific ratios. Figure 3 shows the results of evaluating the elastase inhibitory activity of red houttuynia cordata at different concentrations. Figure 4 shows the results of evaluating the tyrosinase inhibitory activity of red houttuynia cordata at different concentrations. Specific details for implementing the invention

[0073] One or more specific examples are described in more detail below through embodiments. However, these embodiments are intended to illustrate one or more specific examples and the scope of the present invention is not limited to these embodiments.

[0075] Preparation Example 1: Preparation of flower, leaf, and stem extracts of Houttuynia cordata

[0076] 1-1. Preparation of Red-striped Houttuynia cordata flower extract

[0077] 50 g of dried red-patterned Houttuynia cordata flowers were extracted by adding 10 times the amount of 70% ethanol solution and refluxing for 3 hours. The extract was filtered, concentrated using a rotary evaporator, and then freeze-dried to prepare the product.

[0079] 1-2. Preparation of Red-spotted Houttuynia Cordata Leaf Extract

[0080] 50 g of dried red-patterned Houttuynia cordata leaves were extracted with 10 times the amount of 70% ethanol solution under reflux for 3 hours. The extract was filtered, concentrated using a rotary evaporator, and then freeze-dried to prepare the product.

[0082] 1-3. Preparation of Red-spotted Houttuynia Cordata Stem Extract

[0083] 50 g of dried red-patterned Houttuynia cordata stems were extracted by adding 10 times the amount of 70% ethanol solution and refluxing for 3 hours. The extract was filtered, concentrated using a rotary evaporator, and then freeze-dried to prepare the product.

[0085] Preparation Example 2: Preparation of a complex of red-spotted Houttuynia cordata flower, leaf, and stem extracts

[0086] A complex was prepared by mixing each extract prepared in Preparation Example 1 as shown in Table 1. Specifically, the freeze-dried powders of each extract prepared in Preparation Example 1 were quantified and mixed, and the complex was prepared by dissolving them in DMSO at a concentration of 50 mg / ml.

[0087] division Flower:Leaf:Stem Mixture Ratio Flower extract (weight%) Leaf extract (weight%) Stem extract (weight%) Comparative Example 1 1: 0: 0 100 0 0 Comparative Example 2 0: 1: 0 0 100 0 Comparative Example 3 0: 0: 1 0 0 100 Example 1 1: 1: 0 50 50 0 Example 2 1: 0: 1 50 0 50 Example 3 0: 1: 1 0 50 50 Example 4 1: 1: 1 33.33 33.33 33.33 Example 5 2: 1: 1 50 25 25 Example 6 1: 2: 1 25 50 25 Example 7 1: 1: 2 25 25 50 Example 8 1: 2: 3 16.67 33.33 50 Example 9 1: 3: 2 16.67 50 33.33 Example 10 2: 1: 3 33.33 16.67 50 Example 11 2: 3: 1 33.33 50 16.67 Example 12 3: 1: 2 50 16.67 33.33 Example 13 (Red Houttuynia cordata) 3: 2: 1 50 33.33 16.67

[0090] Experimental Example 1: Confirmation of the Antioxidant Effect of a Complex of Red-Spotted Houttuynia Cordata Flowers, Leaves, and Stems

[0091] The antioxidant efficacy of the extracts of Comparative Examples 1 to 3 and the complexes of Examples 1 to 13 presented in Table 1 was confirmed by DPPH (2,2-diphenyl-1-picryl hydrezyl) radical scavenging activity.

[0092] Specifically, the extracts of Comparative Examples 1 to 3 and the complexes of Examples 1 to 13, each with a concentration of 5 μg / ml, were mixed with a 0.1 mM DPPH solution diluted in 4 ml of ethanol. The mixture was left at room temperature for 30 minutes, after which the absorbance was measured at 517 nm. For the untreated group, a DPPH solution without any mixture was used, and for the control group, ascorbic acid, which is used as a conventional antioxidant, was used at the same concentration.

[0093] As a result of absorbance measurement, it was found that among the extracts of Comparative Examples 1 to 3 and the complexes of Examples 1 to 13, Example 13 (Red Houttuynia cordata) had the best antioxidant efficacy (Fig. 1).

[0094] In the following, the compound with the optimal mixing ratio that showed the highest efficacy through antioxidant efficacy experiments (Example 13) was described as "Red Houttuynia cordata," and additional experiments were conducted with Red Houttuynia cordata.

[0096] Experimental Example 2: Confirmation of the Antioxidant Effect of Red Houttuynia Cordata

[0097] The antioxidant effect of red Houttuynia cordata according to concentration was further confirmed. Specifically, red Houttuynia cordata of different concentrations was mixed with a 0.1 mM DPPH solution diluted in 4 ml of ethanol. After leaving the mixture at room temperature for 30 minutes, the absorbance was measured at 517 nm.

[0098] Measurement results confirmed that the DPPH radical scavenging activity increased in a concentration-dependent manner with red houttuynia cordata (Fig. 2).

[0100] Experimental Example 3: Confirmation of the wrinkle and elasticity improvement effects of Red Houttuynia cordata

[0101] The wrinkle and elasticity-improving effects of red houttuynia cordata were evaluated based on elastase inhibitory activity.

[0102] Elastase is an enzyme that breaks down elastin, a protein that maintains skin elasticity; inhibiting the activity of elastase can enhance skin elasticity and, consequently, lead to wrinkle improvement.

[0103] Elastase at a concentration of 0.5 U / ml (stock concentration) and N-succinyl-(L-Ala)3-p-nitroanilide at a concentration of 2 mM (stock concentration) were each dissolved in 50 mM tris-HCl buffer. Red Houttuynia cordata was mixed at different concentrations in 80 μL of tris-HCl buffer.

[0104] The above red Houttuynia cordata, 30 μL of elastase, and 30 μL of N-succinyl-(L-Ala)3-p-nitroanilide were mixed and reacted for 10 minutes. Afterward, the absorbance was measured at 410 nm. Ursolic acid was used as a positive control.

[0105] As a result of the measurement, it was found that the elastase activity inhibitory effect of red houttuynia cordata increased in a concentration-dependent manner (Fig. 3). These results indicate that red houttuynia cordata has a skin elasticity-enhancing effect and, consequently, a wrinkle-improving effect.

[0107] Experimental Example 4: Confirmation of the whitening and pigmentation improvement effects of Red Houttuynia cordata

[0108] The whitening and pigmentation improvement effects of red Houttuynia cordata were evaluated based on tyrosinase enzyme inhibitory activity. Specifically, tyrosinase inhibitory activity was confirmed by measuring DOPA chrome, which is produced as a result of tyrosinase action, using a colorimetric method.

[0109] 110 μL of 0.1 M phosphate buffer (pH 6.5) was mixed with 40 μL of 1.5 mM L-tyrosine solution and 10 μL of red Houttuynia cordata at different concentrations. 10 μL of mushroom tyrosinase at a concentration of 2000 unit / mL was added, and the mixture was reacted at 37°C for 10 minutes. Subsequently, the absorbance was measured at 490 nm. Kojic acid at the same concentration was used as a positive control.

[0110] Measurement results confirmed that the tyrosinase activity inhibitory effect increased in a concentration-dependent manner with red Houttuynia cordata (Fig. 4). This result indicates that red Houttuynia cordata is effective in whitening and improving pigmentation.

Claims

Claim 1 Red-patterned Houttuynia cordata Houttuynia cordata var. purpurea A cosmetic composition for improving skin condition comprising an ethanol extract of ) as an active ingredient, wherein the ethanol extract is a mixture of flower, leaf, and stem ethanol extracts in a weight ratio of 3:2:1, and the skin condition improvement is improvement of skin wrinkles, improvement of skin elasticity, improvement of pigmentation, or skin whitening. Claim 2 delete Claim 3 delete Claim 4 delete Claim 5 delete Claim 6 delete Claim 7 delete

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