Cosmetic composition for improving damaged hair, hair loss and scalp health

Abstract

The present invention relates to a cosmetic composition for improving damaged hair, hair loss, and scalp health. A cosmetic composition for improving damaged hair, hair loss, and scalp health according to one embodiment of the technical concept of the present invention comprises polydeoxyribonucleotide (PDRN), placenta extract, vitamin B5 (panthenol), squalane, complex nanoliposomes, sodium alginate, natural plant extract, Maris Sal (Dead Sea Salt), niacinamide, beta-glucan, allantoin, glycerin, propanediol, glyceryl caprylate, ascorbic acid, sodium anisate, 1,2-hexanediol, and polyglyceryl-10 It contains caprylate (Polyglyceryl-10 Caprylate), Gellan Gum (LA) (Gellan Gum (Low-Acyl)) and purified water (Aqua). A cosmetic composition for improving damaged hair, hair loss, and scalp health according to various embodiments of the technical concept of the present invention, based on the above-described composition, can improve scalp health by soothing the scalp and alleviating skin inflammation, and prevent hair loss by strengthening hair roots, while exhibiting excellent effects on promoting hair growth and hair regeneration, and the growth of damaged hair, without any side effects or toxicity to the human body.

Description

Technology Field

[0001] The present invention relates to a cosmetic composition for improving damaged hair, hair loss, and scalp health, and more specifically, to a cosmetic composition for improving damaged hair, hair loss, and scalp health that can soothe the scalp and alleviate skin inflammation to improve scalp health and prevent hair loss by strengthening hair roots, and exhibits excellent effects on promoting hair growth and hair regeneration and the growth of damaged hair without side effects or toxicity to the human body. Background Technology

[0003] Hair is a modified epidermal cell and is considered an accessory organ of the skin along with sweat glands and sebaceous glands. Numerous SS bonds form cross-links between peptide chains, and the number of hairs varies depending on the person and hair color, with black hair having about 100,000 hairs.

[0004] The hair growth rate is reported to be 0.44 mm / day for the crown of a man's head and 0.39 mm / day for the side hair. When observing the structure of hair in a cross-section of the skin, the epidermis forms a cavity that is indented toward the dermis, which is called a follicle. A sebaceous gland is attached to the upper part of the follicle, where sebum is secreted to provide moisture and protection to the scalp and hair.

[0005] Unlike fingernails, hair does not undergo continuous growth; instead, each individual hair has an independent lifespan and undergoes a cycle of growth, shedding, and regrowth, which is called the hair cycle. Specifically, it is divided into three phases: the growth phase (anagen), the regression phase (catagen), and the resting phase (telogen), and hair is formed only during the growth phase. During this period, the hair papilla is large and hair matrix cells move actively, causing the hair to elongate and the hair bulb to extend into the subcutaneous tissue. Once growth ceases, the hair follicle enters the regression phase, after which cell proliferation within the hair follicle decreases and eventually stops.

[0006] Afterwards, most of the hair follicle, excluding the hair follicle itself, is phagocytosed by surrounding macrophages and contracts, and the hair root shortens (to 1 / 2 to 1 / 3 of the length of the growing hair follicle) down to the point where the arrector pili muscle begins, and enters a resting phase. In the resting phase of the hair follicle, the hair germ, which is the seed for future hair regeneration, interacts with the hair papilla, actively divides and proliferates, differentiates into hair matrix cells, and the next new hair is born.

[0007] The new hair that grows and naturally falls out is the shed hair, and the number is about 70 to 120 per day for coarse hair, the hair growth phase lasts 5 to 6 years, the regression phase lasts 2 to 3 weeks, and the resting phase lasts 2 to 3 months.

[0008] The causes of hair loss can be diverse. First, male pattern baldness, which is an aesthetic issue resulting from thinning hair, is caused by the miniaturization of hair, where the hair becomes thinner and shorter. In other words, it is the miniaturization of coarse hair due to the shortening of the hair cycle's growth period.

[0009] Furthermore, excessive dandruff is broken down by bacteria, and the resulting decomposition products irritate the scalp, causing inflammation such as itching. If left untreated, this can lead to hair loss. Additionally, excessive sebum secretion from the sebaceous glands above the hair follicles is decomposed by scalp commensal bacteria, and the excessive irritation of the scalp by these decomposition products can cause seborrheic alopecia.

[0010] Furthermore, the sympathetic nervous system, which is overactive due to stress, constricts capillaries, weakening the supply of nutrients to the hair and skin, thereby shortening the hair growth phase and leading to hair loss.

[0011] Furthermore, in modern society that prioritizes beauty, many people of all ages and genders use hair dyes and perms to satisfy their desire for individual expression; however, for some individuals with sensitive scalps, these chemical treatments cause irritation and inflammation, such as itching and stinging, which leads to environmental hair loss.

[0012] Accordingly, research is continuing on ingredients that can protect the scalp from environmental stimuli, stress, and chemical treatments caused by various factors, prevent hair loss, promote hair growth, and are eco-friendly and harmless to both the environment and humans. Furthermore, there is a growing demand for cosmetic compositions that are free from problems such as causing economic burdens or suffering, inducing side effects, or relying on only temporary or fragmentary pharmacological mechanisms; instead, these compositions offer superior and sustained efficacy without side effects. Prior art literature

[0014] Korean Registered Patent No. 10-2782612 (Registered March 12, 2025) Korean Registered Patent No. 10-2443708 (Registered September 8, 2022) Korean Registered Patent No. 10-2093543 (Registered March 19, 2020) The problem to be solved

[0015] The problem that the present invention aims to solve is to provide a cosmetic composition for improving damaged hair, hair loss, and scalp health that can soothe the scalp, alleviate skin inflammation to improve scalp health, and prevent hair loss by strengthening hair roots, while having no side effects or toxicity to the human body and exhibiting excellent effects in promoting hair growth and hair regeneration, and in the growth of damaged hair.

[0016] In addition, another problem that the present invention aims to solve is to provide a cosmetic composition for improving damaged hair, hair loss, and scalp health that can maximize the effect of hair growth by promoting blood circulation in the scalp, activating hair follicle cells, inhibiting lipid secretion in the scalp, and supplying nutrients to the hair, has an excellent feel, causes minimal skin irritation, has excellent scalp soothing, dryness and itching prevention effects, prevents dandruff, and has a superior skin soothing effect against inflammatory reactions in the scalp, thereby soothing damaged skin.

[0017] The various problems that the present invention aims to solve are not limited to those mentioned above, and other unmentioned problems will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0019] In one embodiment of the technical concept of the present invention, a cosmetic composition for improving damaged hair, hair loss, and scalp health is disclosed.

[0020] The above cosmetic composition for improving damaged hair, hair loss, and scalp health comprises polydeoxyribonucleotide (PDRN), placenta extract, vitamin B5 (panthenol), squalane, complex nanoliposomes, sodium alginate, natural plant extracts, Maris Sal (Dead Sea Salt), niacinamide, beta-glucan, allantoin, glycerin, propanediol, glyceryl caprylate, ascorbic acid, sodium anisate, 1,2-hexanediol, and polyglyceryl-10 caprylate. It contains Caprylate, Gellan Gum (LA) (Low-Acyl) and purified water (Aqua).

[0021] 1 to 5 parts by weight of the above polydeoxyribonucleotide (PDRN), 1 to 3 parts by weight of placenta extract, 0.5 to 2.5 parts by weight of vitamin B5 (panthenol), 0.1 to 1 part by weight of squalane, 1 to 3 parts by weight of complex nanoliposomes, 0.5 to 1.5 parts by weight of sodium alginate, 0.5 to 1.5 parts by weight of natural plant extract, 0.1 to 1 part by weight of Maris Sal (Dead Sea Salt), 0.1 to 0.5 parts by weight of niacinamide, 0.1 to 0.3 parts by weight of beta-glucan, 0.3 to 0.7 parts by weight of allantoin, glycerin It may be included in a weight ratio of 1 to 5 parts by weight, 1 to 3 parts by weight of propanediol, 0.1 to 0.5 parts by weight of glyceryl caprylate, 0.05 to 0.35 parts by weight of ascorbic acid, 0.1 to 0.5 parts by weight of sodium anisate, 0.1 to 1 part by weight of 1,2-hexanediol, 0.15 to 0.35 parts by weight of polyglyceryl-10 caprylate, 0.01 to 0.2 parts by weight of Gellan Gum (Low-Acyl) (LA), and 80 to 120 parts by weight of purified water (Aqua).

[0022] 3 parts by weight of the above polydeoxyribonucleotide (PDRN), 2 parts by weight of placenta extract, 1.5 parts by weight of vitamin B5 (panthenol), 0.5 parts by weight of squalane, 2 parts by weight of complex nanoliposomes, 1 part by weight of sodium alginate, 1 part by weight of natural plant extract, 0.5 parts by weight of Maris Sal (Dead Sea Salt), 0.3 parts by weight of niacinamide, 0.2 parts by weight of beta-glucan, 0.5 parts by weight of allantoin, 3 parts by weight of glycerin, 2 parts by weight of propanediol, 0.3 parts by weight of glyceryl caprylate, and ascorbic acid It may be included in a weight ratio of 0.2 parts by weight of Ascorbic Acid, 0.3 parts by weight of Sodium Anisate, 0.5 parts by weight of 1,2-Hexanediol, 0.25 parts by weight of Polyglyceryl-10 Caprylate, 0.1 parts by weight of Gellan Gum (Low-Acyl) (LA), and 100 parts by weight of purified water (Aqua).

[0023] Specific details of other embodiments are included in the detailed description. Effects of the invention

[0025] A cosmetic composition for improving damaged hair, hair loss, and scalp health according to various embodiments of the technical concept of the present invention can improve scalp health by soothing the scalp and alleviating skin inflammation, and prevent hair loss by strengthening hair roots, while exhibiting excellent effects on promoting hair growth and the growth of damaged hair without side effects or toxicity to the human body.

[0026] In addition, the cosmetic composition for improving damaged hair, hair loss, and scalp health according to various embodiments of the technical concept of the present invention can maximize the effect of hair growth by promoting blood circulation in the scalp, activating hair follicle cells, inhibiting lipid secretion in the scalp, and supplying nutrients to the hair, has an excellent feel, causes minimal skin irritation, has excellent scalp soothing, and prevents dryness and itching, prevents dandruff, and has an excellent skin soothing effect against inflammatory reactions in the scalp, thereby soothing damaged skin.

[0027] It will be fully understood that embodiments of the technical concept of the present invention may provide various effects not specifically mentioned. Brief explanation of the drawing

[0029] Figures 1a, 2a, and 3a are photographs showing the condition of a subject's scalp before using the cosmetic composition prepared according to Example 1, and Figures 1b, 2b, and 3b are photographs showing the condition of a subject's scalp after using the composition prepared according to Example 1 once a day for 6 months. Figures 4a, 5a, 6a, and 7a are photographs showing the condition of a subject's hair before using the cosmetic composition prepared according to Example 1, and Figures 4b, 5b, 6b, and 7b are photographs showing the condition of a subject's hair after using the cosmetic composition prepared according to Example 1 once a day for 6 months. Specific details for implementing the invention

[0030] The advantages and features of the present invention, and the methods for achieving them, will become clear by referring to the embodiments described in detail below. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosed content is thorough and complete, and to ensure that the spirit of the present invention is sufficiently conveyed to those skilled in the art.

[0031] The terms used in this application are used merely to describe specific embodiments and are not intended to limit the invention. The singular expression includes the plural expression unless the context clearly indicates otherwise.

[0032] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by those skilled in the art to which the present invention pertains. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and should not be interpreted in an ideal or overly formal sense unless explicitly defined in this application.

[0034] Hereinafter, a cosmetic composition for improving damaged hair, hair loss, and scalp health according to one embodiment of the technical concept of the present invention will be described in detail with reference to preferred embodiments.

[0036] A cosmetic composition for improving damaged hair, hair loss, and scalp health according to one embodiment of the technical concept of the present invention comprises polydeoxyribonucleotide (PDRN), placenta extract, vitamin B5 (panthenol), squalane, complex nanoliposomes, sodium alginate, natural plant extract, Maris Sal (Dead Sea Salt), niacinamide, beta-glucan, allantoin, glycerin, propanediol, glyceryl caprylate, ascorbic acid, sodium anisate, 1,2-hexanediol, and polyglyceryl-10 It contains caprylate (Polyglyceryl-10 Caprylate), Gellan Gum (LA) (Gellan Gum (Low-Acyl)) and purified water (Aqua).

[0038] Specifically, a cosmetic composition for improving damaged hair, hair loss, and scalp health according to one embodiment of the technical concept of the present invention comprises 1 to 5 parts by weight of polydeoxyribonucleotide (PDRN), 1 to 3 parts by weight of placenta extract, 0.5 to 2.5 parts by weight of vitamin B5 (panthenol), 0.1 to 1 part by weight of squalane, 1 to 3 parts by weight of complex nanoliposomes, 0.5 to 1.5 parts by weight of sodium alginate, 0.5 to 1.5 parts by weight of natural plant extract, 0.1 to 1 part by weight of Maris Sal (Dead Sea Salt), 0.1 to 0.5 parts by weight of niacinamide, and 0.1 to 0.3 parts by weight of beta-glucan. 0.3 to 0.7 parts by weight of allantoin, 1 to 5 parts by weight of glycerin, 1 to 3 parts by weight of propanediol, 0.1 to 0.5 parts by weight of glyceryl caprylate, 0.05 to 0.35 parts by weight of ascorbic acid, 0.1 to 0.5 parts by weight of sodium anisate, 0.1 to 1 part by weight of 1,2-hexanediol, 0.15 to 0.35 parts by weight of polyglyceryl-10 caprylate, 0.01 to 0.2 parts by weight of gellan gum (LA) (Low-Acyl) and It may be included in a weight ratio of 80 to 120 parts by weight of purified water (Aqua).

[0040] The above polydeoxyribonucleotide (PDRN) is manufactured using DNA extracted from salmon sperm and contains a deoxyribonucleotide polymer of 50 to 2,000 base pairs. PDRN is primarily used for the treatment of wounds and tissue repair resulting from skin grafts, but it is also used at the discretion of medical professionals for the regeneration of certain tissues or the treatment of inflammation where there is no suitable treatment, and it is known to be used in the treatment of a wide range of disorders such as diabetic foot ulcers, scars, vascular insufficiency, and female pattern hair loss.

[0041] In addition, the above-mentioned polydeoxyribonucleotide (PDRN) is known to be a component that stimulates A2 receptors, which are skin regeneration signaling molecules, to promote the secretion of various growth factors, the formation of capillaries by VEGF (vascular endothelial growth factor), the improvement of blood circulation, anti-inflammatory effects, and the prevention of capillary leakage.

[0042] In addition, the polydeoxyribonucleotide (PDRN) can alleviate inflammation in damaged tissues and promote cell regeneration. For example, the polydeoxyribonucleotide (PDRN) can interact with adenosine receptors to promote the expression of vascular endothelial growth factor, thereby enhancing wound healing and promoting the growth of epithelial cells. It can also promote the maturation of the extracellular matrix and increase the density of microvessels, thereby promoting angiogenesis.

[0043] In the present invention, the polydeoxyribonucleotide (PDRN) helps to repair damaged cells and promotes cell growth, thereby helping to increase the regenerative capacity and vitality of scalp tissue. It also helps to promote the production of collagen and elastin in the dermal layer, which can contribute to improving scalp elasticity and strengthening the scalp weakened by aging. Furthermore, through its anti-inflammatory action, it can help to improve inflammatory hair loss or the sensitivity of a sensitized scalp, and help to restore the scalp barrier damaged by inflammation to a healthy state.

[0044] In addition, when the above-mentioned polydeoxyribonucleotide (PDRN) is effectively delivered to the scalp, it can help alleviate and prevent hair loss symptoms by improving the scalp condition that is weakened by aging or stress, and by helping to improve the scalp environment and collagen synthesis, it can be expected to promote the growth of thicker, more elastic, and shiny hair, and it can make the hair itself healthy by improving hair protein damage and hair shine.

[0046] The above-mentioned placenta extract is obtained by extracting active ingredients from the placenta of mammals and contains abundant proteins, amino acids, nucleic acids, vitamins, minerals, growth factors, etc., so it can be used as a functional ingredient effective for skin and scalp care.

[0047] In the present invention, the placenta extract can promote scalp health and regeneration. Since the placenta is rich in growth factors and essential amino acids essential for cell proliferation and recovery, it helps maintain a healthy state by supplying vitality and nutrition to scalp cells damaged by the external environment or aging. Additionally, it helps soothe and restore sensitive or troubled scalps by providing antioxidant effects that protect cells from free radicals and anti-inflammatory actions that alleviate inflammatory responses in the scalp.

[0048] Furthermore, the growth factors contained in placenta extract stimulate the activity of hair matrix cells responsible for hair production, thereby aiding in hair growth. Additionally, the placenta extract contributes to improving microcirculation in the scalp, facilitating the supply of necessary nutrients and oxygen to hair follicles, which helps prevent hair loss. As the scalp environment becomes healthier and nutrient supply becomes smoother, hair roots are strengthened, helping to prevent hair from falling out easily.

[0049] In addition, the abundant proteins and amino acids of the placenta extract can contribute to the recovery of damaged hair itself. For example, since the proteins and amino acids that are the main components of the placenta extract have a structure similar to keratin, the main component of hair, they can penetrate between the cuticle and cortex layers of damaged hair to help repair hair damage and improve elasticity. They can also help supply and maintain moisture to the hair, thereby contributing to making dry, brittle hair soft and shiny.

[0051] The above-mentioned Vitamin B5 (Panthenol) can simultaneously perform the roles of a humectant and an emollient. It attracts surrounding moisture to increase the moisture content of the scalp and maintains hydration by preventing moisture evaporation. It can also strengthen the scalp skin barrier to prevent moisture loss (transepidermal water loss) and protect the scalp from external stimuli. Furthermore, it has an anti-inflammatory effect that alleviates inflammatory reactions, helping to relieve sensitive scalp, redness, and itching. It also helps regenerate damaged scalp epidermal cells and accelerates the speed of wound healing, making it beneficial for the recovery of scalp troubles or micro-wounds. As an ingredient notified by the Ministry of Food and Drug Safety of the Republic of Korea, it nourishes the scalp and provides a hair protection effect, so it can be incorporated into functional cosmetics for alleviating hair loss symptoms.

[0052] In addition, the above-mentioned vitamin B5 (Panthenol) forms a protective film on the surface of the hair to trap moisture and protect the hair from environmental stress, smooths the hair cuticle to reflect light better, improves shine and reduces frizz, penetrates deep into the hair to replenish moisture and increase hair volume, strengthens the hair structure to prevent breakage and helps to cultivate healthy and elastic hair, softens the hair and reduces tangling to make combing easier, and is also effective against static electricity.

[0054] The aforementioned squalane is a substance stabilized by adding hydrogen to squalene, a natural component also found in human sebum. While squalene is unstable and oxidizes easily, squalane has high stability, making it suitable for use as a cosmetic ingredient.

[0055] The above squalane has excellent cleansing power and skin affinity, and is similar to the sebum components of the skin, helping to effectively dissolve oil-based makeup and impurities. Additionally, because its particles are very small, it penetrates deep into the skin and is effective in removing impurities from within the pores. It also has excellent moisturizing and softening effects, forming a protective barrier that prevents moisture loss so that the skin does not dry out even after cleansing, and absorbs lightly and quickly, making the skin soft and moist without stickiness.

[0056] In addition, the aforementioned squalane is gentle on the skin and suitable for sensitive skin. In particular, using squalane in the form of a cleansing oil or cleansing balm reduces friction on the skin, allowing for gentle cleansing, and helps strengthen the skin barrier as it is similar to the lipids that make up the skin's natural protective layer.

[0058] The above-mentioned complex nanoliposome refers to a nanoliposome stabilized by encapsulating resveratrol, camellia oil, ceramide, etc. Resveratrol is a plant-derived polyphenol (phenolic antioxidant) component that is mainly used in the cosmetics field for antioxidant, scalp environment improvement, and skin protection purposes. Camellia Japonica Seed Oil contains a high concentration of over 80% oleic acid (Omega-9) and is traditionally evaluated as a plant-based oil optimized for hair care. The above-mentioned ceramide can provide functions such as protecting the skin barrier, maintaining moisture, suppressing inflammation, and repairing damage.

[0059] In the present invention, the composite nanoliposome comprises: (A) a step of preparing an aqueous phase by mixing 8 to 12 parts by weight of a peptide mixture, 3 to 7 parts by weight of adenosine, 15 to 25 parts by weight of ethanol, 1 to 5 parts by weight of resveratrol, 5 to 10 parts by weight of propylene glycol, and 3 to 7 parts by weight of zinc gluconate with 60 to 80 parts by weight of purified water at a temperature of 65 to 75°C, based on the total weight of the aqueous phase and the oil phase for preparing the nanoliposome;

[0060] (B) A step of preparing an oil phase by mixing 10 to 20 parts by weight of phospholipid, 4 to 8 parts by weight of camellia oil, 1 to 3 parts by weight of tocopherol acetate, 4 to 8 parts by weight of ceramide, 2 to 6 parts by weight of dimethicone, and 1 to 5 parts by weight of phytosteryl derivative at a temperature of 60 to 70°C, based on the total weight of the aqueous and oil phases for preparing nanoliposomes;

[0061] (C) a step of preparing water-in-oil (W / O) type nanoliposomes by heating the aqueous phase prepared in step (A) to a temperature of 50 to 60°C, adding the oil phase prepared in step (B), emulsifying using a homomixer at 4,000 to 5,000 rpm for 10 to 30 minutes, cooling to room temperature, and then performing high-pressure emulsification using a microfluidizer at 900 to 1,100 Bar conditions 3 to 5 times; and

[0062] (D) The water-in-oil (W / O type) nanoliposomes prepared in step (C) above can be prepared by including the step of mixing the aqueous phase from which the active ingredient has been removed from the aqueous phase of step (A) above and the oil phase of step (B) above, and emulsifying using a homomixer at 4,000 to 5,000 rpm for 10 to 30 minutes at 50 to 60°C to prepare a multi-emulsion type (W / O / W type) nanoliposome emulsion.

[0063] In step (D) above, water-in-oil (W / O) nanoliposomes may be included in a ratio of 10 to 20 weight percent relative to the total weight of the nanoliposome emulsion.

[0065] The above peptide mixture refers to a mixture composed of 2 to fewer than 30 amino acids, which are constituent units of proteins. For example, as the above peptide mixture, one or more selected from the group consisting of rh-oligopeptide, human oligopeptide-1, rh-polypeptide-1, acetyl hexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-5, and caffeoyl tripeptide-1 may be used.

[0066] Preferably, rh-oligopeptide can be used as the peptide mixture, and the rh-oligopeptide can perform the role of improving skin wrinkles as an epidermal growth factor and a cell regeneration factor.

[0068] The adenosine mentioned above acts directly on dermal papilla cells responsible for hair growth, which can help extend the hair growth phase (anagen phase) and delay entry into the regression phase (catagen phase) in which the hair follicle shrinks. By facilitating microcirculation in the scalp through vasodilation, it promotes the active supply of oxygen and nutrients to the hair follicles, thereby contributing to the creation of an optimal environment for hair growth. It also helps hair grow healthily by promoting the production of hair growth factors, such as fibroblast growth factor (FGF-7), which is essential for hair growth.

[0069] In addition, the adenosine mentioned above promotes the metabolic activity of scalp cells and enhances cell regenerative capacity to aid in the recovery of damaged scalp. As a functional ingredient for wrinkle improvement, it helps promote collagen synthesis and maintain elasticity in the scalp, and by improving blood circulation in the scalp, it ensures that hair receives sufficient nutrition, thereby helping to improve overall hair health and shine.

[0071] The above-mentioned resveratrol is a powerful antioxidant of the polyphenol family found in grape skins, peanuts, and Polygonum multiflorum. It is a type of phytoalexin produced by plants to protect themselves from external environmental stress or attacks (fungi, pests, etc.). The above-mentioned resveratrol has a stronger antioxidant capacity than vitamins C and E and effectively removes free radicals that damage scalp cells and hair follicles. By doing so, it protects the scalp from oxidative stress that promotes the aging of hair follicles and hinders hair growth, and can help delay scalp aging by suppressing oxidative stress.

[0072] In addition, the above-mentioned resveratrol is It alleviates redness and itching of the scalp by inhibiting inflammation-inducing mechanisms (such as NF-κB) and creates an environment where hair follicles can grow healthily. Some studies suggest that the topical application of resveratrol can have a positive effect on hair growth by promoting the transition of the hair cycle from the telogen phase to the anagen phase. It also supports healthy hair growth by helping dermal papilla cells (DPCs) proliferate and protects them from oxidative damage. Furthermore, it helps improve microcirculation in the scalp to facilitate the supply of oxygen and nutrients to hair follicles. Additionally, it contributes to maintaining the structural health of the scalp by inhibiting the activity of collagen-degrading enzymes induced by UV rays, and may indirectly help protect hair proteins.

[0073] In the present invention, the resveratrol obtained from the stem extract of Polygonum cuspidatum can be used. The stem and root extracts of Japanese Knotweed (scientific name: Polygonum cuspidatum or Reynoutria japonica) are major natural sources of resveratrol. Although resveratrol was originally discovered in grape skins and is widely known, the source most widely utilized in industry is the root and stem extract of Japanese Knotweed.

[0075] The chemical formula of the above propylene glycol is C3H8O2, its specific gravity is 1.036 to 1.040, and its boiling point is 185 to 189°C. The above propylene glycol mixes well with water, alcohol, acetone, ethyl acetate, chloroform, ether, etc., has excellent solubility, and has good hygroscopicity, so it can be used as a moisture evaporation barrier.

[0077] The above zinc gluconate is a form in which the mineral zinc is combined with gluconic acid, which has high bioavailability. Zinc is one of the essential minerals of the human body and is involved in various biological processes such as enzyme activation, cell growth, immune function, and wound healing, and can play an important role in improving seborrheic scalp and hair loss.

[0078] The above zinc gluconate is very effective for managing oily and troubled scalps. Zinc helps suppress excessive sebum secretion by regulating the activity of enzymes that produce sebum, and through its anti-inflammatory action, it helps soothe the scalp and alleviate redness and itching caused by inflammatory scalp conditions or irritation. It also helps inhibit the growth of microorganisms such as Malassezia, one of the causative agents of seborrheic dermatitis, which can be useful for improving dandruff and seborrheic scalps.

[0079] In addition, the zinc gluconate supports hair growth, and zinc contributes to inhibiting the activity of 5-alpha-reductase, an enzyme that converts testosterone into DHT (dihydrotestosterone), which is a major cause of male pattern baldness, and can help promote the recovery of damaged scalp tissue and maintain a healthy scalp barrier.

[0081] The above phospholipid is an ester compound of phosphoric acid and includes natural and synthetic phospholipids, and both saturated and unsaturated types can be used, preferably including phospholipids having a fatty acid chain having 12 to 30 carbon atoms. For example, the above phospholipid may be one or more selected from the group consisting of phosphatidylcholine (PC; natural or synthetic lecithin), dipalmitoylphosphatidylcholine, phosphatidic acid (PA), lysophosphatidylcholine (LPC), phosphatidylserin (PS), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylinositol (PI).

[0083] The above Camellia Japonica Seed Oil contains a high concentration of over 80% oleic acid (Omega-9), which is highly effective in providing deep moisture and nutrition to the hair and scalp. Oleic acid triglycerides, the main component of Camellia Japonica Seed Oil, penetrate into the hair to nourish damaged hair, form a protective barrier to prevent moisture loss, fill in the gaps between the cuticle layers on the hair surface to smooth out loose cuticles, restore shine and gloss to damaged hair, and improve ease of combing. Additionally, Camellia Japonica Seed Oil has a high smoke point, which can help minimize heat damage to the hair during treatments such as blow-drying or heat perming.

[0084] In addition, the above camellia oil prevents scalp dryness and provides a moisturizing effect, helps alleviate dandruff, itching, and sensitivity caused by dryness, contributes to soothing an irritated scalp through anti-inflammatory action, and is rich in vitamins A, B, and E and antioxidants, which can help prevent scalp aging by nourishing the scalp and protecting cells from free radicals.

[0086] The above tocopherol acetate is generally referred to as vitamin E acetate and is a derivative form that stabilizes tocopherol (active vitamin E), a powerful antioxidant vitamin. It has higher stability than pure tocopherol, so it is not easily oxidized in cosmetic compositions, and when absorbed into the skin, it is converted into active tocopherol by enzymes to exert its effects.

[0087] The aforementioned tocopherol acetate reduces oxidative stress. Reactive oxygen species, generated by ultraviolet rays, pollutants, and stress, damage scalp cells and hair follicles, causing hair loss and accelerating aging. Through its powerful antioxidant action, the tocopherol acetate neutralizes these reactive oxygen species, helping to reduce oxidative stress in hair follicles. By protecting scalp cells from oxidative damage, it contributes to slowing down the rate of scalp aging and maintaining a healthy scalp environment. Furthermore, by preventing the oxidation of sebum on the scalp, it lowers the likelihood of scalp problems or inflammation and provides a protective effect on the scalp.

[0089] The above ceramide refers to a compound composed of sphingosine and fatty acids, and is also called N-acylsphingosine. The above ceramide is a major component of the stratum corneum of the epidermal layer of the skin. For example, the above ceramide may include one or more selected from the group consisting of ceramide EOP, ceramide NS, ceramide NP, ceramide AS, and ceramide AP. The above ceramide can fill the spaces between phospholipids in the particle walls of a nanoemulsion of one aspect and create a rigid particle wall of the nanoemulsion.

[0090] In other words, the aforementioned ceramide is essential for maintaining a barrier function in the stratum corneum, the outermost layer of the skin including the scalp. By acting as a 'cement' that fills the spaces between keratinocytes, the ceramide strengthens the scalp barrier, thereby blocking the penetration of external harmful substances (pollution, irritation, etc.) and effectively preventing internal moisture from evaporating. Furthermore, as the barrier function is strengthened, it reduces moisture loss from the scalp, relieving dryness. Additionally, the strengthening of the scalp barrier and moisturization contribute to maintaining scalp health and elasticity, which can indirectly provide a hair loss prevention effect.

[0091] In addition, the ceramide can protect and adhere to the hair cuticle. The cuticle layer, which is the outermost layer of the hair, consists of multiple layers of scales, and ceramide is one of the intercellular binding substances that fills the spaces between these cuticle cells to increase adhesion. When the ceramide component of the hair is lost due to physicochemical damage such as dyeing, perming, or heat, the hair becomes rough and loses its shine. The ceramide helps repair damaged hair by replenishing the lost lipids and improves the ease of combing by smoothing the hair surface. When the damage to the cuticle layer is restored and the surface becomes even, the shine and gloss of the hair are improved, providing a healthy-looking appearance.

[0093] The above-mentioned dimethicone is a silicone-based ingredient primarily used to improve usability and protect the skin. It protects the hair from external environmental factors (heat, friction, UV rays, etc.) by forming a thin, uniform protective film on the hair surface, particularly on cuticles that are lifted or damaged due to damage. It also provides immediate shine and gloss to the hair by smoothly coating the hair surface to increase light reflection, reduces friction on rough and brittle damaged hair to provide a soft touch, effectively prevents hair from tangling, and significantly improves combability. Furthermore, the film formed on the hair surface prevents moisture evaporation from within the hair, thereby preventing dryness.

[0094] In addition, the above-mentioned dimethicone improves the spreadability of the cosmetic composition and provides a fresh and smooth feel without stickiness, thereby increasing product satisfaction. Furthermore, the coating film can play an auxiliary role in allowing other active ingredients included in the composition (e.g., PDRN, adenosine, placenta extract, etc.) to remain on the hair or scalp for a longer period.

[0096] The above-mentioned phytosteryl derivative is a lauroyl glutamate with phytosteryl and octyldodecyl groups added, which has excellent moisturizing effects and plays a role in regenerating damaged skin. It penetrates the intercellular lipid layer of the scalp to help repair and strengthen the weakened scalp barrier. By strengthening the barrier, it effectively prevents internal moisture loss (TEWL) within the scalp, thereby contributing to maintaining the scalp's moisturization state for a long time and alleviating itching or sensitivity caused by dryness. It also helps to make the lipid layer healthy and stabilize it, which can help other active ingredients included in the composition to be better absorbed into the scalp and remain there for a longer period.

[0097] In addition, the above-mentioned phytosteryl derivative acts similarly to the lipid components of the cuticle, the outer layer of the hair, helping to reduce hair damage by filling and repairing gaps in the damaged cuticle layer; it penetrates the hair fibers to provide elasticity and flexibility, smooths the hair surface to provide a soft touch, reduces irregularities in the hair surface to prevent diffuse reflection of light and increases specular reflection to add natural shine and gloss, and indirectly helps lay the foundation for hair growth by soothing an irritated scalp and creating a healthy scalp environment through the anti-inflammatory and soothing effects of the plant sterol itself.

[0098] For example, the above phytosteryl derivative may be one or more selected from the group consisting of phytosteryl / octyldodecyl lauroyl glutamate, phytosteryl macadamiate, phytosteryl oleate, and phytosteryl isostearyl dimer dilinoleate.

[0100] The above sodium alginate is a natural polysaccharide extracted from brown algae (seaweed), and is mainly used to enhance the formulation stability of a composition by acting as a thickener, emulsifying stabilizer, and gelling agent, and can also provide moisturizing and protective functions in cosmetic compositions for improving hair and scalp.

[0101] In the present invention, the sodium alginate has excellent ability to absorb and bind a greater amount of moisture than its own weight, which helps to supply and maintain moisture to the scalp and hair, and by forming a thin, moist moisture film on the surface of the hair and scalp, it provides a sealing effect that prevents moisture evaporation, thereby alleviating scalp dryness and protecting the hair, and by supplying moisture to dry hair and forming a protective film, it makes the hair soft and flexible, thereby reducing tangling of damaged hair and making it easy to manage.

[0102] In addition, the sodium alginate mentioned above increases the viscosity of the composition to provide an appropriate texture to the cosmetic composition and helps other ingredients to disperse evenly, thereby improving the stability of the cosmetic composition. It also has the property of forming a gel-like film, which can play an auxiliary role in helping other active ingredients in the composition to be slowly released or remain on the scalp or hair for a long time, and the alginate component has the property of adsorbing some heavy metals or waste products, which can indirectly help with the cleansing effect on the scalp.

[0103] In the present invention, the sodium alginate may be prepared through the following steps: (1) a brown algae preparation step, (2) a first washing step of brown algae, (3) a step of cutting brown algae and mixing with purified water, (4) a pH adjustment step, (5) a step of adding an enzyme complex, (6) a second washing step of brown algae, (7) a step of separating and purifying impurities, (8) a step of adding and stirring calcium chloride solution, (9) a third washing step of brown algae, (10) a step of adding and stirring hydrochloric acid solution, (11) a fourth washing step of brown algae, (12) a step of adding and stirring sodium carbonate solution, (13) a centrifugation step, and (14) a drying step.

[0104] (1) Brown algae preparation step

[0105] The above brown algae preparation step is a step of preparing brown algae, which are seaweeds containing alginic acid.

[0106] The above alginic acid is a specific component of brown algae and is a substance that constitutes the outer layer of the cell wall. It consists of a structure in which two types of uronic acids, mannuronic acid and gluronic acid, are linked in a 1,4-linkage. Alginic acid is abundant in seaweeds such as wakame, kelp, hijiki, Sargassum, and Ecklonia cava, and is known to be contained in an amount of 10 to 30% by dry weight.

[0107] For example, in the brown algae preparation step, one or more brown algae selected from the group consisting of wakame, kelp, hijiki, Sargassum, and Ecklonia cava may be used as the brown algae, and in addition to the brown algae, various known types of brown algae containing alginic acid may be used.

[0108] (2) First washing step for brown algae

[0109] The above first washing step of the brown algae is a step of washing the brown algae first by immersing it in purified water.

[0110] In the first washing step of the brown algae above, foreign substances remaining on the brown algae can be removed by immersing the brown algae in purified water for the first washing.

[0111] (3) Step of cutting brown algae and mixing with purified water

[0112] The above step of cutting brown algae and mixing with purified water is a step of cutting the first washed brown algae and then mixing with purified water.

[0113] In the above step of cutting brown algae and mixing with purified water, the efficiency of separating and purifying impurities can be improved by cutting the first washed brown algae. For example, in the step of cutting brown algae and mixing with purified water, the first washed brown algae can be cut into units of 1 to 5 cm, and then mixed with purified water at a weight ratio of 300 to 400 parts by weight to 100 parts by weight of the total content of the cut brown algae.

[0114] (4) pH adjustment step

[0115] The above pH adjustment step is a step of adjusting the pH of the brown algae mixed with the above purified water.

[0116] In the above pH adjustment step, an environment suitable for enzyme growth can be created by adjusting the pH of the brown algae mixed with the purified water. For example, in the above pH adjustment step, the pH can be adjusted to have a range of 5 to 6 by adding citric acid to the brown algae mixed with the purified water.

[0117] (5) Addition step of enzyme complex

[0118] The step of adding the enzyme complex is a step of adding the enzyme complex to the pH-controlled brown algae to perform enzymatic decomposition.

[0119] As the enzyme complex added in the step of adding the enzyme complex above, an enzyme complex composed of polygalacturonase, pectin lyase, and cellulase may be used, wherein the enzyme complex is mixed in a weight ratio of 30 to 70 parts by weight of polygalacturonase, 10 to 30 parts by weight of pectin lyase, and 1 to 5 parts by weight of cellulase, and the enzyme complex may be added to have a concentration of 1 to 3 (w / w)%.

[0120] In the step of adding the enzyme complex, the polygalacturonase and pectin lyase are enzymes that dissolve intercellular substances and decompose pectin, which is a binding substance between plant cells, and the cellulase is a cell wall decomposing enzyme that can decompose cellulose present in plant cell walls. For example, in the step of adding the enzyme complex, the enzymatic decomposition can be carried out by maintaining the brown algae to which the enzyme complex has been added at a temperature of 25 to 35°C for 1 to 3 hours.

[0121] (6) Secondary washing step for brown algae

[0122] The above second washing step of brown algae is a step of second washing the enzymatically decomposed brown algae.

[0123] In the second washing step of the brown algae above, the enzymatically decomposed brown algae can be washed using purified water in which sodium bicarbonate (NaHCO3) is dissolved. For example, in the second washing step of the brown algae above, the second washing can be carried out by washing the surface of the enzymatically decomposed brown algae with purified water in which sodium bicarbonate (NaHCO3) has a concentration range of 1 to 3 (w / w)%.

[0124] In the second washing step of the brown algae mentioned above, the sodium bicarbonate (NaHCO3) is also used as a food additive, is non-toxic, has functions such as penetration, diffusion, and expansion, and can sterilize by expanding the cell walls of mold.

[0125] (7) Impurity separation and purification step

[0126] The above impurity separation and purification step is a step of removing impurities contained in the brown algae by mixing a solvent composition with the secondary washed brown algae.

[0127] That is, in the impurity separation and purification step, the purity of the extracted alginic acid can be improved by mixing a solvent composition with the secondary washed brown algae to remove impurities, namely various pigments, contained in the brown algae. In the impurity separation and purification step, the solvent composition may be prepared by mixing a first solvent composition for separating and purifying pigments having polar groups contained in the brown algae, and a second solvent composition for separating and purifying pigments having low polarity or non-polar groups. The solvent composition may be mixed in a weight ratio of 30 to 70 weight% of the first solvent composition and 30 to 70 weight% of the second solvent composition.

[0128] In addition, in the impurity separation and purification step, pigments contained in the secondary washed brown algae can be separated and purified by mixing a solvent composition in a weight ratio of 200 to 400 parts by weight to 100 parts by weight of the total content of the secondary washed brown algae, and maintaining it at a temperature of 25 to 35°C for 30 to 90 minutes.

[0129] In addition, in the impurity separation and purification step, the first solvent composition may be one or more selected from ethanol or methanol, and the second solvent composition may be one or more selected from the group consisting of DME (Dimethyl Ether), IPA (Isopropyl Alcohol), and Diethyl Ether.

[0130] Generally, various raw materials including wakame, kelp, and Sargassum are used to extract alginic acid from brown algae, but pigments present in the brown algae are also extracted along with the alginic acid product, so separation and purification of these are required.

[0131] The substances containing polar groups (e.g., fucoxanthin, chlorophyll, etc.) contained in the above brown algae have high solubility in ethanol and can be effectively separated, but other substances have low solubility in ethanol alone as a solvent and must undergo several process steps.

[0132] Therefore, the purity of alginic acid can be increased by separating and purifying pigments having polar and non-polar structures using a solvent composition in which a first solvent composition for separating and purifying pigments having polar groups and a second solvent composition for separating and purifying pigments having low polarity or non-polar groups are mixed in a certain ratio.

[0133] For example, among the pigments contained in brown algae, non-polar pigments include carotenes (β-carotene, etc.) which are fat-soluble and have a hydrocarbon structure, intermediate polar pigments include fucoxanthin and xanthophyll which contain oxygen groups and have ester groups, and polar pigments include chlorophyll a / b and phycobilins which contain polar groups (-COOH, -OH, -Mg-).

[0134] Among the first solvent compositions above, ethanol has the chemical formula C2H5OH and a relative polarity index (where the polarity index is a relative polarity indicator, and the higher the index, the stronger the polarity) of 4.3 (medium), and can dissolve fucoxanthin, some carotenoids, etc., and when separating pigments contained in brown algae, various pigments can be extracted due to the medium polarity and high safety for the human body, so it can be widely used for extraction for food and cosmetics.

[0135] In addition, the above methanol has the chemical formula CH3OH, a relative polarity index of 5.1 (high), and can dissolve fucoxanthin, chlorophyll, polar pigments, etc. Due to its strong polarity, it has excellent dissolving power for polar pigments when separating pigments contained in brown algae, and is effective for extracting chlorophyll and fucoxanthin.

[0136] Among the second solvent compositions above, DME (Dimethyl Ether) has the chemical formula CH3OCH3 and a relative polarity index of 2.8 (weakly polar to non-polar), and can dissolve carotenes, xanthophyll, etc., and can selectively dissolve non-polar pigments (carotenes) when separating pigments contained in brown algae, and has a boiling point of -24℃, which is advantageous for heat-sensitive pigments.

[0137] In addition, the above IPA (Isopropyl Alcohol) has the chemical formula (CH3)2CHOH, a relative polarity index of 3.9 (medium), and can dissolve fucoxanthin, chlorophyll, and a small amount of carotene. Due to its medium polarity, it can partially dissolve both polar and non-polar pigments when separating pigments contained in brown algae, and is also useful for removing protein and sugar impurities.

[0138] In addition, the above-mentioned Diethyl Ether (Diethyl Ether, Ethyl Ether) has the chemical formula (C2H5)2O, a relative polarity index of 2.8 (non-polar), and can dissolve carotenes, lipid pigments, etc. It has an excellent effect on dissolving lipid-soluble pigments and lipids when separating pigments contained in brown algae, and can also help inhibit the destruction of Mg-porphyrin centers within Chlorophyll.

[0139] (8) Calcium chloride solution addition and stirring step

[0140] The above step of adding and stirring the calcium chloride solution is a step of adding the brown algae from which impurities have been removed to a calcium chloride (CaCl2) solution and then stirring.

[0141] In the above calcium chloride mixing and stirring step, the brown algae from which impurities have been removed are added to a calcium chloride (CaCl2) solution and stirred, thereby dissociating the polymer bonds within the alginic acid and removing some of the water-soluble substances (such as fucoidan) and pigments present in the brown algae from which impurities have been removed.

[0142] That is, in the calcium chloride mixing and stirring step, the calcium chloride (CaCl2) combines with the carboxyl group of alginic acid to convert the alginic acid into an insoluble salt (Ca-alginic acid), which is Ca 2+Through ion exchange, the tissue can be stabilized and water-soluble components can be extracted, and the cell walls of the brown algae can be fixed and aggregated to facilitate the removal of impurities (proteins, mucus, etc.).

[0143] Specifically, the calcium chloride mixing and stirring step can be carried out by adding the brown algae from which impurities have been removed to an aqueous solution of calcium chloride (CaCl2) of 0.5 to 2.5 weight% and stirring at a speed of 150 to 250 RPM for 10 to 20 hours.

[0144] (9) Third washing step of brown algae

[0145] The above third washing step of the brown algae is a step of washing the brown algae, which has been introduced into and stirred in the above calcium chloride (CaCl2) solution, with deionized water (DI Water) for the third time.

[0146] In the third washing step of the brown algae above, the brown algae that is introduced into and stirred with the calcium chloride (CaCl2) solution is washed a third time, thereby removing water-soluble impurities such as residual salt, protein, and mucilage after treatment with the calcium chloride (CaCl2) solution. The deionized water (DI Water) washes away salts, sugars, proteins, low molecular weight pigments, etc., and prevents the residue of chemical reagents such as CaCl2, HCl, and Na2CO3. Additionally, by using deionized water (DI water), unnecessary ion exchange can be prevented and pH stability can be maintained.

[0147] (10) Addition and stirring of hydrochloric acid solution

[0148] The above hydrochloric acid solution addition and stirring step is a step of adding the above 3rd washed brown algae to a hydrochloric acid (HCl) solution and stirring.

[0149] In the above step of adding and stirring the hydrochloric acid solution, the tertiary washed brown algae are added to the hydrochloric acid (HCl) solution and stirred, thereby converting insoluble Ca-alginate into an insoluble free acid form (H-alginate), wherein the hydrochloric acid (HCl) is Ca 2+ It removes (Ca-alginate + 2HCl → H2-alginate + CaCl2), elutes and removes metal ion impurities (Mg, Fe, etc.), and can also perform an auxiliary role in removing inorganic salts remaining in the tissue and decolorizing.

[0150] Specifically, the hydrochloric acid solution addition and stirring step can be carried out by adding the third washed brown algae to a 3 to 7 weight percent aqueous hydrochloric acid (HCl) solution and stirring at a speed of 150 to 250 RPM for 30 to 90 minutes.

[0151] (11) Fourth washing step of brown algae

[0152] The above fourth washing step of the brown algae is a step of washing the brown algae, which has been introduced into the hydrochloric acid (HCl) solution and stirred, with deionized water (DI Water) for the fourth time.

[0153] In the above-mentioned fourth washing step (S1100) of the brown algae, the brown algae that has been introduced into and stirred in the above-mentioned hydrochloric acid (HCl) solution is washed a fourth time to remove residual HCl and generated CaCl2 and metal salts after acid treatment, and to restore the pH to neutral so as not to affect the subsequent alkali treatment (dissolution step).

[0154] (12) Addition and stirring of sodium carbonate solution

[0155] The above step of adding and stirring the sodium carbonate solution is a step of adding the above-mentioned 4th washed brown algae to a sodium carbonate (Na2CO3) solution and stirring.

[0156] In the step of adding and stirring the sodium carbonate solution, the brown algae washed four times are added to a sodium carbonate (Na2CO3) solution and stirred to convert and dissolve the insoluble H2-alginate produced by acid treatment into a Na-salt form (Na-alginate) for extraction, and the sodium carbonate (Na2CO3) acts as a basic solution (weak alkali) to induce the following reaction.

[0157]

[0158] Specifically, the step of adding and stirring the sodium carbonate solution can be carried out by adding the 4th washed brown algae to an aqueous solution of 1 to 3 weight percent sodium carbonate (Na2CO3) and stirring at a speed of 150 to 250 RPM for 10 to 20 hours.

[0159] (13) Centrifugation step

[0160] The above centrifugation step is a step of preparing a sodium alginate solution by centrifuging brown algae that has been introduced into and stirred with the sodium carbonate (Na2CO3) solution and obtaining the supernatant.

[0161] The above centrifugation step is a step for separating the extracted Na-alginate (solution) from insoluble residues (cellulose, proteins, pigments, etc.). The supernatant contains dissolved Na-salt of alginate (i.e., extracted water-soluble sodium alginate), and solid impurities (residual seaweed) are precipitated using the difference in specific gravity, thereby allowing the supernatant containing the target component, the sodium alginate solution, to be separated and obtained.

[0162] (14) Drying step

[0163] The above drying step is a step of preparing sodium alginate by heating and drying the sodium alginate solution.

[0164] In the above drying step, sodium alginate contained in the sodium alginate solution can be obtained by heating and drying the sodium alginate solution. Since the configuration for heating and drying the sodium alginate solution is a known technique, a detailed description thereof will be omitted for the convenience of explanation and to clarify the technical concept of the present invention.

[0166] The above natural plant extract can be used by mixing Scutellaria root extract and Paeonia bark extract in a 1:1 weight ratio, and the above natural plant extract can produce a very strong synergy for anti-inflammatory, soothing, and antioxidant effects on the scalp.

[0167] The above Scutellaria Baicalensis Root Extract is extracted from the root of Scutellaria Baicalensis, a plant of the Lamiaceae family. Thanks to flavonoid components including baicalin, which is the main component, it can provide powerful anti-inflammatory and soothing effects to the scalp and hair. The main components of Scutellaria Baicalensis are known to inhibit the activation of NF-κB, an inflammation-inducing pathway, which helps to effectively soothe a sensitive and inflamed scalp and is useful for relieving scalp troubles, redness, and itching.

[0168] In addition, the above-mentioned Scutellaria Baicalensis Root Extract has excellent antibacterial activity, which contributes to inhibiting the proliferation of harmful bacteria on the scalp and maintaining a clean scalp environment. It is also utilized as a natural preservative that replaces chemical preservatives to enhance product stability. Furthermore, as a powerful antioxidant, it protects scalp cells from free radicals, prevents aging, aids in scalp regeneration, and has been reported to inhibit 5α-reductase related to hair loss, so it can be utilized in compositions for alleviating hair loss symptoms.

[0169] The above Paeonia Suffruticosa Root Extract is extracted from the root bark of the peony and has traditionally been used for pain relief and anti-inflammatory purposes. Paeonia Suffruticosa also has excellent anti-inflammatory effects, making it effective in quickly soothing irritated or sensitive scalps and alleviating inflammation. It is also known to contain more antioxidant compounds than alpha-tocopherol (vitamin E), which can help prevent scalp aging and maintain a healthy scalp environment.

[0170] In addition, the above-mentioned Paeonia Suffruticosa Root Extract exhibits an inhibitory effect on harmful bacteria that parasitize the scalp, particularly those causing superficial fungal infections, through its antibacterial action, thereby contributing to maintaining scalp cleanliness. This can also help alleviate dandruff or itching. Furthermore, as a raw material for hair cosmetics, it contributes to improving the hair growth environment through its hair protective, antioxidant, antibacterial, and anti-inflammatory effects, and has been studied as an ingredient in hair growth products.

[0172] The above Dead Sea salt (Maris Sal (Dead Sea Salt)) is known to have superior efficacy as it contains significantly higher levels of minerals such as magnesium, calcium, and bromide than ordinary seawater. As a natural mineral raw material that is receiving great attention in beauty and dermatology, it performs various functions such as strengthening the skin barrier, removing toxins, anti-inflammatory effects, moisturizing, and improving cleansing power.

[0173] Specifically, the particles of the above-mentioned Dead Sea salt (Maris Sal) act as a scrub to physically remove waste products such as old dead skin cells, sebum, and fine dust from the scalp, thereby reducing factors that can clog pores and cause hair loss, thus keeping the scalp clean. Additionally, due to the high mineral concentration, it induces an osmotic pressure phenomenon in the scalp to help waste products escape and contributes to purifying the scalp. Furthermore, the Dead Sea salt has a particularly high magnesium content, which can help alleviate inflammatory reactions in the scalp and improve skin barrier function.

[0174] In addition, minerals such as calcium, potassium, and magnesium contained in the above Dead Sea salt (Maris Sal) play an essential role in strengthening the function of the scalp skin barrier and reducing moisture loss, and magnesium improves the moisture retention ability of scalp cells to provide a moisturizing effect to dry and sensitive scalps, and when the Dead Sea salt remains on the hair, it acts finely on the cuticle layer to produce a conditioning effect that gives the hair a slight volume and texture.

[0176] The above-mentioned niacinamide is a water-soluble form of vitamin B3 (niacin) and is a multifunctional ingredient that provides a wide range of effects in cosmetic compositions, such as whitening, anti-aging, skin barrier strengthening, and skin trouble care; it is one of the whitening functional ingredients notified by the Ministry of Food and Drug Safety (MFDS) of the Republic of Korea.

[0177] Specifically, the niacinamide inhibits the pathway of melanin moving from melanocytes to keratinocytes, thereby preventing melanin from rising to the skin surface, which helps to alleviate hyperpigmentation such as melasma, blemishes, and freckles, and improves post-inflammatory hyperpigmentation (PIH) to help make the skin tone even and clear. It also helps restore and strengthen the damaged skin barrier by promoting the synthesis of lipids such as ceramide and free fatty acids, which are major components of the skin barrier, thereby reducing transepidermal water loss (TEWL). As a result, the skin's moisture retention capacity increases, alleviating dryness and keeping the skin softer and healthier.

[0178] In addition, the niacinamide can help prevent pores from widening by regulating sebaceous gland activity and suppressing excessive sebum secretion, and can help reduce pore size by improving pore blockage phenomena such as blackheads and whiteheads. Furthermore, the niacinamide itself has anti-inflammatory properties, which helps soothe skin redness and inflammation caused by acne or other irritations, making it useful for managing sensitive or acne-prone skin. It can also help with collagen synthesis and perform antioxidant functions to protect against skin damage caused by ultraviolet rays or free radicals, alleviate fine lines and wrinkle depth, improve skin elasticity, and improve skin yellowness, a sign of skin aging, thereby contributing to creating younger and more vibrant skin.

[0180] The above-mentioned beta-glucan is a type of natural polysaccharide extracted from the cell walls of yeast, mushrooms, grains (oats, barley), etc., and can be included in cosmetic compositions to provide multifaceted effects such as powerful moisturizing, soothing, skin immunity enhancement, and anti-aging.

[0181] Specifically, the above-mentioned beta-glucan is one of the humectants, and it has excellent ability to attract and bind water molecules, supplying and maintaining moisture not only to the surface of the skin but also to deep layers, thereby relieving dryness and internal dryness, and forming a thin protective film on the surface of the skin to minimize transepidermal water loss (TEWL), keeping the skin moist and supple, and it has a strong anti-inflammatory effect, and contributes to regulating the skin immune system by helping to activate macrophages, which are skin immune cells, and can quickly soothe skin that has become red or sensitive due to external stimuli or environmental stress and alleviate discomfort.

[0182] In addition, the above-mentioned beta-glucan acts as an antioxidant component to neutralize free radicals generated by environmental harmful factors (ultraviolet rays, pollution) and acts on the dermis layer to indirectly support collagen synthesis, thereby preventing skin aging and improving skin elasticity to help reduce fine lines and the depth of wrinkles. It can also activate immune cells within the skin to strengthen the skin's natural defense system and enhance the ability to protect the skin from external pathogens or harmful microorganisms.

[0184] The above allantoin acts as a skin protectant and skin conditioning agent, and is particularly effective for soothing the skin, promoting regeneration, moisturizing, and keratolytic effects.

[0185] Specifically, the allantoin mentioned above is a mild ingredient with almost no irritation, and it has anti-inflammatory and soothing effects that effectively relieve discomfort such as redness, itching, and stinging of the skin. It is highly suitable for sensitive and weakened skin by reducing the skin's reaction to external stimuli, and it can contribute to protecting the skin and keeping it smooth by stimulating the proliferation of skin cells, especially keratinocytes, to help repair damaged tissues, thereby promoting skin regeneration such as minor wounds, scratches, and blemishes, and supporting the growth of new healthy skin tissue.

[0186] In addition, the allantoin mentioned above has a humectant property that attracts moisture and helps strengthen the function of the skin barrier by promoting the production of filaggrin proteins that constitute the skin barrier, thereby increasing the skin's moisture content and preventing moisture loss to keep the skin hydrated and supple. It also repairs damaged skin barriers to protect the skin from the external environment and has a keratolytic property that gently loosens the bonds of old keratinocytes, allowing it to gently remove excess dead skin cells and make the skin surface smooth and supple.

[0188] The above glycerin is a humectant that has excellent ability to attract moisture from the surrounding environment (air) or deep within the skin and retain it on the skin surface (stratum corneum), effectively increasing the moisture content of the skin's stratum corneum to relieve dryness and instantly moisten the skin. Furthermore, the above glycerin mimics the skin's natural moisturizing factor (NMF) to help maintain a healthy skin lipid structure, repair damage to the skin barrier, and minimize trans-epidermal water loss (TEWL), thereby protecting the skin from external stimuli and maintaining skin health.

[0189] In addition, the glycerin can alleviate the skin's reaction to external irritants, reduce itching or discomfort caused by dryness, and help accelerate the wound healing process of damaged skin. It can also be included in cosmetic compositions to act as a solvent or viscosity modifier, help stabilize and disperse active ingredients, and improve the feel and spreadability of cosmetic compositions to provide a smooth application.

[0191] The above propanediol acts as a humectant, similar to glycerin, to attract and retain moisture, and also has emollient properties that form a thin film on the skin to reduce moisture loss, and can make the skin soft and flexible by keeping the skin moist and preventing dryness.

[0192] In addition, the propanediol acts as a viscosity reducing agent to lower the viscosity of the cosmetic composition, thereby improving the texture and usability of the cosmetic composition. When used together with glycerin, it reduces the tackiness, which is a disadvantage of glycerin, and can provide a light, fresh, and spreadable texture.

[0194] The above glyceryl caprylate is a monoester of glycerin and caprylic acid derived from coconut oil or palm oil, and can be included in cosmetic compositions to perform various multifunctional roles.

[0195] Specifically, the glyceryl caprylate has both hydrophilic (water-friendly) and lipophilic (oil-friendly) properties, so it acts as a surfactant (emulsifier) ​​that helps water and oil mix well, and is mainly used as a co-emulsifier that increases stability, provides a soft feel on the skin, and forms a thin film to prevent moisture evaporation, making the skin flexible and smooth.

[0196] In addition, the caprylic acid derivative of the glyceryl caprylate component has weak antimicrobial activity that inhibits the growth of microorganisms (especially some bacteria and yeast), thereby playing a decisive role in preventing microbial contamination in 'preservative-free' or 'natural preservative system' cosmetic compositions that do not use chemical preservatives (such as parabens), or in reducing the amount of preservatives used by enhancing the efficacy of existing preservatives. It also has activity against some acne-related bacteria (Propionibacterium acnes), so it can be used as an auxiliary agent in acne care products. It helps stabilize or control the viscosity of cosmetic compositions and is generally known as a mild ingredient with low skin irritation, making it suitable for products for sensitive skin.

[0198] The above-mentioned ascorbic acid is a powerful water-soluble antioxidant that helps prevent UV-induced skin damage and signs of aging (wrinkles, loss of elasticity) by neutralizing free radicals generated when the skin is exposed to ultraviolet (UV) rays or pollutants, thereby preventing oxidative damage to skin cells. Additionally, by acting as a cofactor essential for collagen synthesis, it promotes the production of collagen and elastin in the dermis layer of the skin, which can help improve skin elasticity and density and alleviate fine lines and deep wrinkles.

[0199] In addition, the above-mentioned ascorbic acid inhibits the activity of the tyrosinase enzyme that produces melanin, thereby reducing melanin production, and reduces already produced melanin pigment to lighten the color, thus alleviating melasma, freckles, blemishes (pigmentation), and red marks after acne (post-inflammatory pigmentation), and shows excellent effects in making the overall skin tone even and bright.

[0201] The above sodium anisate acts as a natural preservative booster and additionally functions as a skin conditioning agent and fragrance, and is a sodium salt of p-anisic acid derived from anise or fennel.

[0202] Specifically, the sodium anisate inhibits the growth of microorganisms (mold, yeast, bacteria, etc.) within cosmetic compositions through strong antibacterial and antifungal action. As a natural ingredient derived from plants, it acts gently even on sensitive skin, making it a preferred preservative system in the manufacture of natural and organic cosmetic compositions. It also helps maintain healthy skin conditions as a skin conditioning agent and, thanks to its mild antibacterial properties, helps maintain the microbial balance on the skin surface, thereby protecting the skin and contributing to the inhibition of the growth of acne-causing bacteria (Propionibacterium acnes).

[0203] In addition, the sodium anisate has a mild scent, so it can impart a soft scent to the cosmetic composition or act as a masking agent to cover up the unpleasant odor of other ingredients.

[0205] The above 1,2-hexanediol prevents the proliferation of bacteria, some yeasts, and molds, thereby extending the shelf life of the product and maintaining safety. It also acts as a humectant and emollient that attracts moisture from the air and prevents moisture evaporation from the skin, thereby supplying and retaining moisture to the skin to alleviate dryness and flaking, making the skin moist and supple, and providing a soft and smooth feel to the skin, which can improve the overall skin feel of the cosmetic composition.

[0207] The above polyglyceryl-10 caprylate is a monoester of polyglycerin-10 and caprylic acid (C8 fatty acid) and belongs to the category of non-ionic surfactants with strong hydrophilicity (water-friendly). It helps maintain a stable emulsion of two phases that do not mix with each other, such as water and oil, and is mainly used in the manufacture of oil-in-water (O / W) emulsions due to its strong hydrophilic portion.

[0208] In addition, the above polyglyceryl-10 caprylate has excellent micelle-forming ability, so it acts as a solubilizer to transparently dissolve small amounts of water-soluble oil-soluble ingredients (e.g., fragrances, some plant extracts, oils) that are not easily soluble in water, and has excellent chemical dispersion ability to help the active ingredient spread evenly throughout the formulation, and can perform a cleansing action as a surfactant by enveloping and removing oil components.

[0210] The above Gellan Gum (LA) (Low-Acyl) is a natural polysaccharide obtained through microbial fermentation, and can be used to create unique and transparent formulations by providing strong gelling and stabilizing functions in cosmetic compositions.

[0211] Specifically, the gellan gum (LA) has very high transparency compared to other gums, making it suitable for manufacturing clear and clean gel-type cosmetic compositions. It also improves the user experience by providing a fresh texture that is firm yet less sticky, and acts as a suspending agent to ensure that insoluble particles in the cosmetic composition are evenly dispersed without settling. Furthermore, it has high stability over a wide pH range and at high temperatures (High Temperature Processing, sterilization processes, etc.), making it easy to use in various manufacturing environments and with active ingredients.

[0212] In addition, the gellan gum (LA) acts as a film-forming agent and skin conditioning agent that forms a thin film on the skin, thereby helping the formed film retain moisture to maintain skin hydration and contributing to holding the active ingredients on the skin surface so that their effects can be sustained.

[0214] The above purified water (Aqua) acts as a solvent to dissolve water-soluble active ingredients, moisturizers, thickeners, etc., into a uniform liquid state. When applied to the skin, it immediately supplies moisture to hydrate skin cells and relieve dryness. It also provides a fresh and light sensation to the cosmetic composition, helping it spread smoothly on the skin. Additionally, when the purified water meets the thickener, it imparts an appropriate viscosity to the formulation, allowing it to be applied stably to the skin without flowing too much.

[0216] Hereinafter, with reference to the attached drawings, an example of a cosmetic composition for improving damaged hair, hair loss, and scalp health according to one embodiment of the technical concept of the present invention will be described in more detail.

[0218] < Example 1 >

[0219] 3 parts by weight of Polydeoxyribonucleotide (PDRN), 2 parts by weight of Placenta Extract, 1.5 parts by weight of Vitamin B5 (Panthenol), 0.5 parts by weight of Squalane, 2 parts by weight of Complex Nanoliposomes, 1 part by weight of Sodium Alginate, 1 part by weight of Natural Plant Extract, 0.5 parts by weight of Maris Sal (Dead Sea Salt), 0.3 parts by weight of Niacinamide, 0.2 parts by weight of Beta-Glucan, 0.5 parts by weight of Allantoin, 3 parts by weight of Glycerin, 2 parts by weight of Propanediol, 0.3 parts by weight of Glyceryl Caprylate, Ascorbic A cosmetic composition was prepared by mixing 0.2 parts by weight of ascorbic acid, 0.3 parts by weight of sodium anisate, 0.5 parts by weight of 1,2-hexanediol, 0.25 parts by weight of polyglyceryl-10 caprylate, 0.1 parts by weight of gellan gum (Low-Acyl) (LA), and 100 parts by weight of purified water (Aqua).

[0220] At this time, the composite nanoliposome comprises: (A) a step of preparing an aqueous phase by mixing 10 parts by weight of a peptide mixture, 5 parts by weight of adenosine, 20 parts by weight of ethanol, 3 parts by weight of resveratrol, 8 parts by weight of propylene glycol, and 5 parts by weight of zinc gluconate with 70 parts by weight of purified water at a temperature of 70°C, based on the total weight of the aqueous phase and oil phase for preparing the nanoliposome; (B) a step of preparing an oil phase by mixing 15 parts by weight of phospholipid, 6 parts by weight of camellia oil, 2 parts by weight of tocopherol acetate, 6 parts by weight of ceramide, 4 parts by weight of dimethicone, and 3 parts by weight of a phytosteryl derivative at a temperature of 65°C, based on the total weight of the aqueous phase and oil phase for preparing the nanoliposome; (C) A step of preparing water-in-oil (W / O) type nanoliposomes by heating the aqueous phase prepared in step (A) to a temperature of 55°C, adding the oil phase prepared in step (B) and emulsifying using a homomixer at 4,500 rpm for 20 minutes, cooling to room temperature, and then performing high-pressure emulsification by permeating four times at 1,000 Bar using a microfluidizer; and (D) the water-in-oil (W / O type) nanoliposomes prepared in step (C) are mixed again with the aqueous phase from which the active ingredient has been removed from the aqueous phase of step (A) and the oil phase of step (B), and emulsified using a homomixer at 4,500 rpm at 55°C for 20 minutes to prepare a multi-emulsion type (W / O / W type) nanoliposome emulsion, thereby ensuring that the water-in-oil (W / O type) nanoliposomes in step (D) are included at a ratio of 15% by weight to the total weight of the nanoliposome emulsion.

[0221] In addition, the sodium alginate was prepared by first washing brown algae mixed with wakame and kelp in a 1:1 weight ratio, immersing the brown algae in purified water, cutting the first-washed brown algae into units of 2.5 to 3.5 cm, and mixing them with purified water at a weight ratio of 350 parts by weight to 100 parts by weight of the total cut brown algae; adjusting the pH to 5.5 by adding citric acid to the brown algae mixed with purified water; enzymatically hydrolyzing the pH-adjusted brown algae by adding an enzyme complex mixed with polygalacturonase in a weight ratio of 50 parts by weight, pectin lyase in 20 parts by weight, and cellulase in 3 parts by weight to achieve a concentration of 2 (w / w); and secondarily hydrolyzing the enzymatically hydrolyzed brown algae with purified water in which sodium bicarbonate (NaHCO3) dissolved at a concentration range of 2 (w / w)%. The brown algae were washed, and 300 parts by weight of a solvent composition mixed in a weight ratio of 50% by weight of a first solvent composition and 50% by weight of a second solvent composition was mixed with 100 parts by weight of the total content of the second-washed brown algae, and maintained at a temperature of 30°C for 60 minutes to separate and purify the pigments contained in the second-washed brown algae, wherein the first solvent composition used ethanol and the second solvent composition used DME (Dimethyl Ether), the brown algae from which impurities had been removed were added to a 1.5% by weight aqueous calcium chloride (CaCl2) solution and stirred at a speed of 200 RPM for 15 hours, the brown algae added to and stirred in the calcium chloride (CaCl2) solution were washed a third time with deionized water (DI Water), and the third-washed brown algae were 5% by weight After being added to an aqueous hydrochloric acid (HCl) solution, it was stirred at a speed of 200 RPM for 60 minutes, and the brown algae added to and stirred in the hydrochloric acid (HCl) solution were subjected to deionized water (D.The brown algae washed four times with water, and the brown algae washed four times were added to a 2 wt% sodium carbonate (Na2CO3) aqueous solution and stirred at a speed of 200 RPM for 15 hours. The brown algae added to and stirred in the sodium carbonate (Na2CO3) solution were centrifuged to obtain the supernatant, thereby preparing a sodium alginate solution. The sodium alginate prepared by heating and drying the sodium alginate solution was used.

[0223] < Example 2 >

[0224] A cosmetic composition was prepared in the same manner as in Example 1, but in Example 2, 4 parts by weight of polydeoxyribonucleotide (PDRN), 1.5 parts by weight of placenta extract, 2.3 parts by weight of vitamin B5 (panthenol), 0.3 parts by weight of squalane, 2.5 parts by weight of complex nanoliposomes, 0.7 parts by weight of sodium alginate, 1.3 parts by weight of natural plant extract, 0.3 parts by weight of Maris Sal (Dead Sea Salt), 0.4 parts by weight of niacinamide, 0.15 parts by weight of beta-glucan, 0.6 parts by weight of allantoin, 2 parts by weight of glycerin, and 2.5 parts by weight of propanediol, A cosmetic composition was prepared by mixing 0.2 parts by weight of glyceryl caprylate, 0.33 parts by weight of ascorbic acid, 0.2 parts by weight of sodium anisate, 0.9 parts by weight of 1,2-hexanediol, 0.2 parts by weight of polyglyceryl-10 caprylate, 0.18 parts by weight of gellan gum (Low-Acyl) (LA), and 100 parts by weight of purified water (Aqua).

[0226] < Example 3 >

[0227] A cosmetic composition was prepared in the same manner as in Example 1, but in Example 3, 2 parts by weight of polydeoxyribonucleotide (PDRN), 2.5 parts by weight of placenta extract, 1 part by weight of vitamin B5 (panthenol), 0.8 parts by weight of squalane, 1.5 parts by weight of complex nanoliposomes, 1.3 parts by weight of sodium alginate, 0.8 parts by weight of natural plant extract, 0.8 parts by weight of Maris Sal (Dead Sea Salt), 0.2 parts by weight of niacinamide, 0.25 parts by weight of beta-glucan, 0.4 parts by weight of allantoin, 4 parts by weight of glycerin, and 1.5 parts by weight of propanediol, A cosmetic composition was prepared by mixing 0.4 parts by weight of glyceryl caprylate, 0.15 parts by weight of ascorbic acid, 0.4 parts by weight of sodium anisate, 0.3 parts by weight of 1,2-hexanediol, 0.32 parts by weight of polyglyceryl-10 caprylate, 0.08 parts by weight of Gellan gum (Low-Acyl) (LA), and 100 parts by weight of purified water (Aqua).

[0229] < Comparative Example >

[0230] 3 parts by weight of Vitamin B5 (Panthenol), 1 part by weight of Squalane, 2 parts by weight of Centella Asiatica extract, 0.5 parts by weight of Niacinamide, 0.4 parts by weight of Beta-Glucan, 0.6 parts by weight of Allantoin, 4 parts by weight of Glycerin, 3 parts by weight of Propanediol, 0.3 parts by weight of Glyceryl Caprylate, 0.3 parts by weight of Ascorbic Acid, 0.3 parts by weight of Sodium Anisate, 0.8 parts by weight of 1,2-Hexanediol, 0.35 parts by weight of Polyglyceryl-10 Caprylate, A cosmetic composition was prepared by mixing 0.15 parts by weight of Gellan Gum (Low-Acyl) (LA) and 100 parts by weight of purified water (Aqua).

[0232] 1. Stability test

[0233] A stability test was performed on the cosmetic compositions prepared according to the above Examples 1, 2, 3 and Comparative Example.

[0234] The observation period was conducted for one week, and stability was observed at various temperatures. The results are shown in [Table 1].

[0235] temperature Example 1 Example 2 Example 3 Comparative example 0℃ Good Good Good Good 40℃ Good Good Good Good -20℃ Good Good Good Good

[0236] Referring to [Table 1] above, it was confirmed that the cosmetic products prepared according to Examples 1, 2, 3 and Comparative Example had good formulation stability.

[0238] 2. Dandruff reduction rate

[0239] Men and women with relatively large amounts of dandruff were selected, and 10 people were tested by having them use the cosmetic compositions according to Examples 1, 2, and 3 and the Comparative Example for one month. Before the start of the test, hair was washed with the same conventional shampoo, and dandruff accumulated over 3 days was collected. The weight of the collected dandruff was compared with the weight of dandruff accumulated over 3 days after the end of the test, by washing hair once a day with the cosmetic compositions according to Examples 1, 2, and 3 and the Comparative Example. The results are shown in [Table 2] below.

[0240] Dandruff Reduction Rate (%) = [(Weight of dandruff before start of test (mg) – Weight of dandruff 1 month after start of test (mg)) / Weight of dandruff before start of test (mg)] × 100

[0241] Changes in hair due to use Dandruff reduction rate (%) Change in hair thickness (mm) Hair density (hairs / cm²) 2 ) Scalp itching relief effect Example 1 44.7 0.018 198 8.6 Example 2 43.4 0.017 192 8.5 Example 3 43.6 0.017 195 8.6 Comparative example 18.6 0.001 176 4.3

[0242] Referring to [Table 2] above, it was found that the dandruff reduction rate was about twice as high when washing hair with the cosmetic composition according to Examples 1, 2, and 3 compared to when washing hair with the cosmetic composition according to the Comparative Example.

[0244] 3. Antioxidant Activity Experiment

[0245] Radical scavenging activity was measured using the cosmetic compositions of Examples 1, 2, 3 and Comparative Example as samples.

[0246] The antioxidant activity (electron donation ability, EDA%) of the sample was measured using a modified Blois method. 2 mL of the sample was mixed with 2 mL of 0.2 mM DPPH (1,1-diphenyl-2-picryl hydrazyl) solution, left to stand for 30 minutes, and then the absorbance was measured at 517 nm.

[0247] Radical scavenging activity was expressed as the rate of reduction in absorbance between the sample-added group and the sample-free group. Radical scavenging activity was calculated using the following formula, and the results are shown in [Table 3] below. BHT (synthetic antioxidant) was used as the control sample.

[0248] Radical scavenging activity (%) = (1 - Absorbance of sample-added group / Absorbance of control group) × 100

[0249] division Radical scavenging activity Example 1 58.5 ± 0.4 Example 2 58.2 ± 0.5 Example 3 57.9 ± 0.5 Comparative example 32.5 ± 0.4 Control group 62.2 ± 0.4

[0250] Referring to [Table 3] above, the cosmetic compositions prepared according to Examples 1, 2, and 3 were found to have antioxidant activity and were confirmed to have an effect similar to the radical scavenging ability of a conventional synthetic antioxidant used as a control.

[0251] Therefore, it can be confirmed that the cosmetic composition prepared according to Examples 1, 2, and 3 has an excellent antioxidant effect that improves the scalp against active oxygen and free radicals, and has excellent anti-inflammatory, soothing, and antioxidant effects on the scalp.

[0253] 4. Measurement of inflammation-relieving effects

[0254] The anti-inflammatory effects of the cosmetic compositions according to Examples 1, 2, and 3 and the Comparative Example were measured, respectively. First, macrophages (Raw 2647 mouse cells) were placed in a 96-well microplate at a rate of 4 × 10⁶ per well. 5 After dispensing cells, each of the compositions of Examples 1, 2, 3 and Comparative Example was treated at a concentration of 1,000 μg / mL for 24 hours at 37°C under 5% CO2 conditions. After treatment, the supernatant was taken and the amount of TNF-α was measured using the sandwich ELISA method, and the results are shown in [Table 4] below.

[0255] division TNF-α assay Example 1 Example 2 Example 3 Comparative example TNF-α expression rate (%) 67.0 64.0 65.0 41.0

[0256] Referring to [Table 4] above, it can be seen that the cosmetic compositions according to Examples 1, 2, and 3 exhibit a higher TNF-α induction effect compared to the cosmetic composition according to the Comparative Example, and that the cosmetic compositions according to Examples 1, 2, and 3 have an excellent immune-enhancing effect by increasing the activity of macrophages through the induction of TNF-α production.

[0258] 5. Observation of scalp condition

[0259] Hair was washed once a day using the cosmetic composition prepared according to Example 1, and the condition of the subject's scalp was observed after curing for a certain period.

[0261] Figures 1a, 2a, and 3a are photographs showing the condition of a subject's scalp before using the cosmetic composition prepared according to Example 1, and Figures 1b, 2b, and 3b are photographs showing the condition of a subject's scalp after using the composition prepared according to Example 1 once a day for 6 months.

[0263] Referring to FIGS. 1a to 3b, it can be seen that the scalp condition (Fig. 1b, Fig. 2b, 3b) after a subject uses the composition prepared according to Example 1 is less dandruff and the scalp cleanliness is improved and the hair is soft and shiny compared to the scalp condition (Fig. 1a, Fig. 2a, 3a) before using the cosmetic composition prepared according to Example 1.

[0265] 6. Observation of hair condition

[0266] Hair was washed once a day for 6 months using the cosmetic composition prepared according to Example 1, and the condition of the subject's hair was observed after 6 months.

[0268] Figures 4a, 5a, 6a, and 7a are photographs showing the condition of a subject's hair before using the cosmetic composition prepared according to Example 1, and Figures 4b, 5b, 6b, and 7b are photographs showing the condition of a subject's hair after using the cosmetic composition prepared according to Example 1 once a day for 6 months.

[0270] Referring to FIGS. 4a to 7b, it was confirmed that after using the cosmetic composition prepared according to Example 1, the subject's hair was restored to being thick and healthy.

[0272] Although a preferred embodiment of the present invention has been described above with reference to the attached drawings, those skilled in the art will understand that the present invention may be implemented in other specific forms without altering its technical concept or essential features. Therefore, the embodiment described above should be understood as illustrative in all respects and not restrictive.

Claims

Claim 1 Polydeoxyribonucleotide (PDRN), Placenta Extract, Vitamin B5 (Panthenol), Squalane, Complex Nanoliposomes, Sodium Alginate, Natural Plant Extracts, Maris Sal (Dead Sea Salt), Niacinamide, Beta-Glucan, Allantoin, Glycerin, Propanediol, Glyceryl Caprylate, Ascorbic Acid, Sodium Anisate, 1,2-Hexanediol, Polyglyceryl-10 Caprylate, Gellan Gum (LA) (Low-Acyl)) and purified water (Aqua), and the composite nanoliposome comprises: (A) a step of preparing an aqueous phase by mixing 10 parts by weight of a peptide mixture, 5 parts by weight of adenosine, 20 parts by weight of ethanol, 3 parts by weight of resveratrol, 8 parts by weight of propylene glycol, and 5 parts by weight of zinc gluconate with 70 parts by weight of purified water at a temperature of 70°C, based on the total weight of the aqueous phase and oil phase for preparing the nanoliposome; (B) A step of preparing an oil phase by mixing 15 parts by weight of phospholipid, 6 parts by weight of camellia oil, 2 parts by weight of tocopherol acetate, 6 parts by weight of ceramide, 4 parts by weight of dimethicone, and 3 parts by weight of phytosteryl derivative at a temperature of 65°C, based on the total weight of the aqueous and oil phases for preparing nanoliposomes;(C) A step of preparing water-in-oil (W / O) type nanoliposomes by heating the aqueous phase prepared in step (A) to a temperature of 55°C, adding the oil phase prepared in step (B) and emulsifying using a homomixer at 4,500 rpm for 20 minutes, cooling to room temperature, and then performing high-pressure emulsification by permeating four times at 1,000 Bar using a microfluidizer; A cosmetic composition for improving damaged hair, hair loss, and scalp health, characterized by using a composite nanoliposome prepared through the process of (D) mixing the water-in-oil (W / O type) nanoliposome prepared in step (C) with the aqueous phase from which the active ingredient has been removed in step (A) and the oil phase from step (B), and emulsifying using a homomixer at 55°C at 4,500 rpm for 20 minutes to prepare a multi-emulsion type (W / O / W type) nanoliposome emulsion, wherein the natural plant extract is a mixture of Scutellaria root extract and Paeonia bark extract in a weight ratio of 1:

1. Claim 2 In claim 1, the above-mentioned polydeoxyribonucleotide (PDRN) 1 to 5 parts by weight, placenta extract 1 to 3 parts by weight, vitamin B5 (panthenol) 0.5 to 2.5 parts by weight, squalane 0.1 to 1 part by weight, complex nanoliposomes 1 to 3 parts by weight, sodium alginate 0.5 to 1.5 parts by weight, natural plant extract 0.5 to 1.5 parts by weight, Maris Sal (Dead Sea Salt) 0.1 to 1 part by weight, niacinamide 0.1 to 0.5 parts by weight, beta-glucan 0.1 to 0.3 parts by weight, and allantoin 0.3 to 0.7 parts by weight. Damage characterized by containing, in a weight ratio of 1 to 5 parts by weight of glycerin, 1 to 3 parts by weight of propanediol, 0.1 to 0.5 parts by weight of glyceryl caprylate, 0.05 to 0.35 parts by weight of ascorbic acid, 0.1 to 0.5 parts by weight of sodium anisate, 0.1 to 1 part by weight of 1,2-hexanediol, 0.15 to 0.35 parts by weight of polyglyceryl-10 caprylate, 0.01 to 0.2 parts by weight of Gellan Gum (Low-Acyl) (LA), and 80 to 120 parts by weight of purified water (Aqua). Cosmetic composition for improving hair, hair loss and scalp health. Claim 3 In claim 2, 3 parts by weight of polydeoxyribonucleotide (PDRN), 2 parts by weight of placenta extract, 1.5 parts by weight of vitamin B5 (panthenol), 0.5 parts by weight of squalane, 2 parts by weight of complex nanoliposomes, 1 part by weight of sodium alginate, 1 part by weight of natural plant extract, 0.5 parts by weight of Maris Sal (Dead Sea Salt), 0.3 parts by weight of niacinamide, 0.2 parts by weight of beta-glucan, 0.5 parts by weight of allantoin, 3 parts by weight of glycerin, 2 parts by weight of propanediol, and 0.3 parts by weight of glyceryl caprylate. A cosmetic composition for improving damaged hair, hair loss, and scalp health, characterized by containing, in a weight ratio of 0.2 parts by weight of ascorbic acid, 0.3 parts by weight of sodium anisate, 0.5 parts by weight of 1,2-hexanediol, 0.25 parts by weight of polyglyceryl-10 caprylate, 0.1 parts by weight of Gellan Gum (Low-Acyl) (LA), and 100 parts by weight of purified water (Aqua).

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