A multi-functional aroma therapy composition that is effective in antidepressants, stress relief, sleep induction, and fatigue recovery

Abstract

The present invention relates to a multifunctional aromatherapy composition that simultaneously exhibits antidepressant, stress-relieving, sleep-inducing, and fatigue-recovering effects, characterized by containing a natural oil prepared by immersing a mixture of the above-ground parts of *Glechoma longifolia* and the above-ground parts of *Geranium thunbergii* in a 1:1 weight ratio in olive oil heated to a temperature of 50 to 60°C for 2 to 4 hours.

Description

Technology Field

[0001] The present invention relates to an aromatherapy composition, and more specifically, to a multifunctional aromatherapy composition that simultaneously exhibits antidepressant, stress relief, sleep induction, and fatigue recovery effects. Background Technology

[0003] Aromatherapy refers to a series of therapeutic academic disciplines that use natural essential oils extracted from various parts (flowers, wood, roots, fruits, peels, etc.) of natural (aromatic) plants to promote and maintain human physical, mental, and psychological health and to enhance vitality. When examining the fragrance delivery system through respiration in aromatherapy using natural essential oils, after fragrance molecules enter the nose, receptors within olfactory nerve cells accept the fragrance molecules. The fragrance molecules then pass through the olfactory nerves and enter the amygdala. The amygdala sends electrical transmission signals to the limbic system of the brain to promote hormone secretion, and the structures of the limbic system, which governs emotions and memories, also undergo a process of being stimulated by the transmission signals.

[0005] These aromatherapy effects include promoting circulation, strengthening the immune system, relieving muscle tension and pain, destroying accumulated fat, eliminating waste products from tissues, increasing relaxation, and promoting deep sleep. An example of this is the aromatherapy essential oil blend composition that helps with skin care and mental health, disclosed in Korean Patent Publication No. 10-2004-0055713.

[0007] However, conventional aromatherapy compositions had the problem that their effects were not complex and multifunctional, so they had to be manufactured with different compositions depending on the application. Prior art literature

[0009] 1. Republic of Korea Published Patent No. 10-2004-0055713 The problem to be solved

[0010] Therefore, the problem that the present invention aims to solve is to provide a multifunctional aromatherapy composition that simultaneously exhibits antidepressant, stress relief, sleep induction, and fatigue recovery effects. means of solving the problem

[0012] To achieve the above objectives, the present invention

[0013] The present invention provides a multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery, characterized by containing a natural oil prepared by mixing the above-ground parts of *Glechoma longifolia* and the above-ground parts of *Geranium thunbergii* in a 1:1 weight ratio and immersing the mixture in olive oil heated to a temperature of 50 to 60°C for 2 to 4 hours.

[0016] The multifunctional aromatherapy composition according to the present invention, which is highly effective for antidepressant, stress relief, sleep induction, and fatigue recovery, may further include sandalwood oil, bergamot oil, and wintergreen oil.

[0018] In a multifunctional aromatherapy composition according to the present invention that is highly effective for antidepressant, stress relief, sleep induction, and fatigue recovery,

[0019] With respect to 100 parts by weight of the above natural oil,

[0020] It is preferable to contain 10 to 30 parts by weight of sandalwood oil, 10 to 30 parts by weight of bergamot oil, and 10 to 30 parts by weight of wintergreen oil.

[0022] More preferably

[0023] With respect to 100 parts by weight of the above natural oil,

[0024] It may contain 15 parts by weight of sandalwood oil, 15 parts by weight of bergamot oil, and 15 parts by weight of wintergreen oil.

[0026] The above-mentioned Acalypha australis used in the present invention is an annual plant of the family Euphorbiaceae, order Geraniales, and class Dicotyledonous plants that grows in fields or meadows. Its scientific name is Acalypha australis. In the present invention, it is preferable to use the above-ground parts of Acalypha australis, which are covered with short hairs, stand upright, and branch out. The leaves are alternate, have petioles, and are egg-shaped or egg-shaped lanceolate with pointed tips. The young shoots are eaten as a vegetable and are also used as livestock feed.

[0028] In addition, the above-mentioned Geranium is a perennial plant of the Geraniaceae family, order Geraniales, and class Dicotyledonous plants that grows in mountains and fields, and its scientific name is Geranium sibiricum. In the present invention, it is preferable to use the above-ground parts of Geranium, which grow obliquely or sideways and branch out, and have downward-pointing hairs along with the leaf stalks. The leaves are opposite, have long leaf stalks, are 4 to 7 cm wide, and are deeply divided in a palmate shape. It is distributed in Korea, China, Japan, Siberia, North America, Europe, etc.

[0030] In addition, the sandalwood oil is an essential oil extracted from the wood and roots of sandalwood, a plant of the sandalwood family, and is composed of partial alcohols, which has excellent anti-inflammatory properties; the bergamot oil is an essential oil extracted from the fresh peel of the bergamot fruit, which brightens the mind and has excellent effects in relieving stress and depression; and the wintergreen oil is an essential oil obtained by steam distilling the leaves of Gaultheria procumbens of the Rhododendron family by soaking them in water at 49°C overnight during the winter to allow the glycoside gaulterin to decompose.

[0032] The present invention has an advanced feature in that it manufactures a new natural oil as described above and combines it with an essential oil known for its efficacy to produce a multifunctional composition that simultaneously exhibits antidepressant, stress-relieving, sleep-inducing, and fatigue-recovering effects. Effects of the invention

[0034] As the composition according to the present invention described above is a multifunctional composition that simultaneously exhibits antidepressant, stress-relieving, sleep-inducing, and fatigue-recovering effects, it offers high consumer satisfaction and convenience in use, and is also very useful as a base composition for manufacturing aromatherapy compositions for various uses. Specific details for implementing the invention

[0036] The present invention will be specifically explained through the following examples and test examples. It is evident that the present invention is not limited by the examples described below.

[0038] Examples 1: Aroma according to the present invention Therapy composition

[0039] A multifunctional aromatherapy composition with excellent antidepressant, stress-relieving, sleep-inducing, and fatigue-recovering effects containing natural oil was prepared by mixing 1000g of a mixture of the above-ground parts of *Glechoma longifolia* and the above-ground parts of *Geranium thunbergii* in a 1:1 weight ratio and immersing it in 1 liter of olive oil heated to a temperature of 55℃ for 3 hours.

[0041] Example 2: Aromatherapy composition according to the present invention

[0042] A multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery was prepared by adding 15 parts by weight of sandalwood oil, 15 parts by weight of bergamot oil, and 15 parts by weight of wintergreen oil to 100 parts by weight of the natural oil of Example 1.

[0044] Test example 1: Antidepressant effect confirmation test

[0045] 1. Preparation of test animals

[0046] In this study, 5-week-old male ICR mice (SPF / VAF CrljBgi: CD-1) were supplied and acclimatized for 7 days, after which they were divided into a control group and a test group that inhaled the composition of the example.

[0048] The test animals were housed in polycarbonate mouse cages in groups of five in a rearing room maintained at a constant temperature of 23 ± 3℃, humidity of approximately 50 ± 10%, and a 12-hour light-dark cycle, and were supplied with food and water without restriction during the adaptation period.

[0050] 2. Statistical Processing

[0051] All test results were statistically analyzed using one-way analysis of variance (ANOVA), and if significance was recognized, the Student-Newman-Keuls Method was used to test for significance at a P < 0.05 level.

[0053] 3. Confirmation of antidepressant activity by forced swimming

[0054] To measure the antidepressant activity of forced swimming, the following test was conducted using the FST, a standardized test method also known as the despair behavior test described in the literature (RD Porsolt, et al., Behavioral despair in mice: a primary screening test for antidepressants, Arch Int Pharmacodyn Ther, 229, pp.327-36, 1977).

[0056] The FST test procedure was conducted following the method of the original proposer, Porsolt et al., and the antidepressant effect was measured using a self-prepared FST after the drug inhalation was completed.

[0058] In this test, a transparent acrylic cylindrical tank with a height of 25 cm and a diameter of 11 cm was constructed, and mice were forcibly immersed in water at 25 ± 1°C at a water level where their tails did not touch the bottom. After allowing them to adapt for the first 2 minutes, the immobility duration time was measured using the EthoVision program for 4 minutes to evaluate the antidepressant effect, and the results are shown in Table 1 below.

[0060] During the first 2 minutes of the test, the mouse showed strong resistance to escape, but as time passed, the time spent in a stationary position increased, and at the end, it maintained its body in a nearly stationary state. A stationary state is a state in which the mouse floats on the water with only a part of its face exposed above the water surface, showing only slight movements to maintain its balance.

[0062] After the FST was finished, the mice were dried and returned to their rearing cages, and the water temperature was always maintained at 25 ± 1℃.

[0064] The composition of the example was inhaled at a dose of 0.5 g / kg starting 3 hours before the start of the test, and the control group was tested without inhalation, with each group consisting of 10 animals.

[0066] Time(sec) control group 182.18 ± 4.48 Example 1 117.25 ± 4.25 Example 2 112.38 ± 4.13

[0068] As shown in Table 1 above, the immobility time of the control group was 182.18 ± 4.48 seconds, and the immobility time of Example 1 of the composition according to the present invention was 117.25 ± 4.25 seconds, and Example 2 was 112.38 ± 4.13 seconds, which was significantly reduced compared to the control group (P < 0.05).

[0070] 4. In the tail suspension test Confirmation of antidepressant activity by

[0071] The measurement of antidepressant activity by the tail suspension test was performed using the method of Steru et al. (Steru L. et al., The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacology 85, pp.367-370, 1985). This method involves observing that mice with their tails suspended alternate between activity and immobility, and since antidepressant drugs reduce the time spent in an immobility state in a dose-dependent manner, antidepressant activity is confirmed by measuring this time.

[0073] The antidepressant effect was measured using a self-made TST after the drug inhalation was finished. In this test, the antidepressant effect was determined by measuring the immobility duration time for 10 minutes after attaching a tape to the edge of a table 50 cm high at a distance of 1 cm from the tip of the mouse's tail.

[0075] The test conditions were the same as those for measuring antidepressant activity by forced swimming, and the results are shown in Table 2.

[0077] Time(sec) control group 218.15 ± 7.23 Salsijye 1 141.53 ± 6.74 Example 2 137.21 ± 5.29

[0079] As shown in Table 2 above, the immobility time of the control group was 218.15 ± 7.23 seconds, and for the composition Example 1 according to the present invention, it was 141.53 ± 6.74 seconds, and for Example 2, it was 137.21 ± 5.29 seconds, which was observed to be significantly reduced compared to the control group (P < 0.05).

[0081] Test example 2: Sleep Effect Verification Test

[0082] 1. Preparation of test animals

[0083] The same mice as in Test Example 1 were used, and the day-night cycle was set to a 12-hour interval one week prior to the test, and the mice were fasted for 24 hours prior to the test to complete the test preparation.

[0085] 2. Statistical Processing

[0086] All test results were statistically analyzed using one-way analysis of variance (ANOVA), and if significance was recognized, the Student-Newman-Keuls Method was used to test for significance at a P < 0.05 level.

[0088] 3. Test to verify sleep effects

[0089] Using test animals prepared by the method described above, the time taken to fall asleep, i.e., sleep latency time, and the time taken to fall asleep, i.e., sleep duration, were measured, and the results are shown in Table 3.

[0091] The sleep induction time was set as the elapsed time until the righting reflex was lost for more than 1 minute, the sleep duration was set as the time until the animal recovered the righting reflex, and animals that did not fall asleep within 15 minutes were excluded from the measurement of test results.

[0093] The composition of the example was administered at a dose of 0.5 g / kg, and the control group was tested without inhalation. Each group consisted of 10 animals.

[0095] Sleep induction time (seconds) Sleep duration (seconds) control group 511.15 ± 13.25 1195.25 ± 13.28 Example 1 368.54 ± 11.28 1518.54 ± 13.36 Example 2 345.25 ± 11.27 1595.25 ± 14.58

[0097] As shown in Table 3 above, the control group showed a sleep induction time of 511.15 ± 13.25 seconds and a sleep duration of 1195.25 ± 13.28 seconds, while Example 1 of the composition according to the present invention showed a sleep induction time of 368.54 ± 11.28 seconds and a sleep duration of 1518.54 ± 13.36 seconds, and Example 2 showed a sleep induction time of 345.25 ± 11.27 seconds and a sleep duration of 345.25 ± 11.27 seconds, confirming a significantly increased effect compared to the control group (P < 0.05).

[0099] 4. Confirmation of re-sleep induction effect

[0100] If one can easily fall back asleep without any processing after waking up due to some stimulus, this means that the stress caused by the stimulus is relieved and the sleep is induced again, which implies that the stress relief and sleep induction effects are excellent.

[0102] The re-sleep induction time refers to the time from when sleep was induced, when sufficient sleep time was not given, when stimulation was applied after about 400 seconds to restore the orienting reflex, until sleep was induced again, just like in the sleep effect verification test described above. The re-sleep induction time was measured and the results are shown in Table 4.

[0104] Sleep induction time (seconds) control group 772.28 ± 13.29 Example 1 551.35 ± 11.25 Example 2 524.71 ± 11.39

[0106] As shown in Table 4 above, the control group showed a sleep-re-induction time of 772.28 ± 13.29 seconds, while the composition Example 1 according to the present invention showed a sleep-re-induction time of 551.35 ± 11.25 seconds, and Example 2 showed 524.71 ± 11.39 seconds, confirming a significant improvement in effect compared to the control group (P < 0.05).

[0108] Test example 3: Efficacy verification test using stress patches

[0109] A test was performed to confirm the stress-relieving effect of the above example composition using a stress patch.

[0111] The test group applied the composition of the example, and the control group applied 3 mg of purified water to the back of the hand, and then attached a purchased stress patch (FTH INC.) to the center of the palm of 200 people (100 in the test group, 100 in the comparison group) for 10 minutes to check the stress level, and the results are shown in Table 5.

[0113] In an unstable state, it appears red, which indicates high stress, while in a stable state, it appears blue, which indicates low stress.

[0115] stability instability control group 68 38 Example 1 92 8 Example 2 97 3

[0117] As shown in Table 5 above, the control group showed a red color indicating a highly stressful and unstable state in 38 people, while the composition Example 1 according to the present invention showed a significantly lower unstable state in 8 people compared to the control group, and Example 2 showed an unstable state in only 3 people, indicating that there is a significant effect in relieving stress.

[0119] Test example 4: Soothes Muscle Fatigue and Recovery efficacy verification test

[0120] Fifty office workers aged 20 to 45 who frequently experienced stiffness, swelling, and pain due to muscle fatigue after returning home were administered 3 mg on the back of their hands three times a day (at 12:00, 19:00, and 22:00) for four weeks each. The effects of soothing swelling, pain, fatigue, and recovery in the leg muscles were scored according to evaluation criteria, and the average of the scores is shown in Table 6 below. Purified water was used as the control group.

[0122] The judgment criteria are as follows:

[0123] 4 points - Very good improvement effect,

[0124] 3 points - Slight improvement effect,

[0125] 2 points - There appears to be a slight improvement, but it is not certain,

[0126] 1 point - No improvement or deterioration,

[0127] 0 points - No improvement, rather worsening.

[0129] rating control group 1.1 Example 1 3.6 Example 2 3.8

[0131] As shown in Table 6 above, it was confirmed that the compositions of Example 1 and Example 2 according to the present invention have an excellent effect on soothing and recovering from muscle fatigue compared to the control group.

[0133] As described above, the composition according to the present invention is a multifunctional composition that simultaneously exhibits antidepressant, stress-relieving, sleep-inducing, and fatigue-recovering effects, so it offers high consumer satisfaction and convenience of use, and is also very useful as a base composition for manufacturing aromatherapy compositions for various uses.

Claims

Claim 1 A multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery, characterized by containing a natural oil prepared by immersing 1,000g of a mixture of the above-ground parts of *Glechoma longifolia* and the above-ground parts of *Geranium thunbergii* in a 1:1 weight ratio in 1 liter of olive oil heated to a temperature of 55℃ for 3 hours. Claim 2 A multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery, characterized in that it further comprises sandalwood oil, bergamot oil, and wintergreen oil in claim 1. Claim 3 A multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery, characterized in that, in claim 2, it contains 10 to 30 parts by weight of sandalwood oil, 10 to 30 parts by weight of bergamot oil, and 10 to 30 parts by weight of wintergreen oil, based on 100 parts by weight of the natural oil. Claim 4 A multifunctional aromatherapy composition with excellent effects in antidepressant, stress relief, sleep induction, and fatigue recovery, characterized in that, in claim 3, it contains 15 parts by weight of sandalwood oil, 15 parts by weight of bergamot oil, and 15 parts by weight of wintergreen oil per 100 parts by weight of the natural oil.

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