Automatic measuring apparatus automatic quantification functions for liquid samples, and automatic analysis method using the same

Abstract

An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may include: a main manifold comprising a plurality of inlets for the inflow of fluid and a valve installed at the inlets to control the movement of the fluid; a detection module connected to the main manifold for the reaction between a reagent and a sample and for the detection of a substance of interest; a first flow path connecting the main manifold and the detection module; a first pump installed in the first flow path to transport the fluid; a sample flow path connected to the first flow path or the main manifold to supply a sample; a first air valve connected to the first flow path to control the supply of air; and a quantitative control unit connected to the main manifold or the first flow path, spaced apart from the first air valve, and quantifying the volume of the liquid between the first air valve and the main manifold.

Description

Technology Field

[0001] The present invention relates to an automatic measuring device having an automatic quantitative function for a liquid sample capable of automatically performing chemical reactions and signal detection for quantitative and qualitative analysis of a substance of interest within a liquid sample, and an automatic analysis method using the same. Background Technology

[0002] The analysis of liquid samples involves processes such as the injection of samples and reagents, chemical reactions, signal detection, wastewater discharge, and washing. To minimize analytical errors, a device capable of automatically performing the aforementioned series of processes is required. Prior art literature

[0003] Registered Patent Publication No. 10-2219833 (February 18, 2021) The problem to be solved

[0004] Based on the technical background described above, the present invention provides an automatic measuring device having an automatic quantitative function for liquid samples that enables accurate control of fluid volume using small amounts of sample and reagents and analysis with high accuracy and precision in a short time, and an automatic analysis method using the same. means of solving the problem

[0005] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may include: a main manifold comprising a plurality of inlets for fluid inflow and a valve installed at the inlets to control the movement of the fluid; a detection module connected to the main manifold for the reaction between a reagent and a sample and the detection of a substance of interest; a first flow path connecting the main manifold and the detection module; a first pump installed in the first flow path to transport the fluid; a sample flow path connected to the first flow path or the main manifold to supply a sample; a first air valve connected to the first flow path to control the supply of air; and a quantitative control unit connected to the main manifold or the first flow path, spaced apart from the first air valve, and quantifying the volume of the liquid between the first air valve and the main manifold.

[0006] According to one aspect of the present invention, the first flow path may include a quantitative flow path formed between the quantitative control unit and the first air valve to receive a sample or standard solution.

[0007] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention further comprises a second air valve connected to the first flow path and controlling the supply of air, wherein the first air valve and the second air valve are spaced apart in the longitudinal direction of the first flow path and can accommodate a diluent between the first air valve and the second air valve.

[0008] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may further include an auxiliary manifold connected to the second air valve to control the flow of a reagent, a diluent, and air.

[0009] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may further include a heater disposed between the detection module and the first pump in the first flow path and heating a sample moving along the first flow path, a first discharge flow path connected to the first flow path for discharging excess sample and excess diluted liquid, and a first discharge control valve for controlling the connection between the first discharge flow path and the first flow path.

[0010] According to one aspect of the present invention, the detection module comprises a light source that irradiates light, a detector that receives light, and an interference filter module disposed between the light source and the detector. The interference filter module may include a plurality of interference filters that selectively transmit only light of a preset wavelength range, a holder that supports the interference filters, and a filter transfer unit that moves the holder.

[0011] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may further include a second flow path connected to the detection module to discharge waste liquid from the detection module or supply air to the detection module, a second pump installed in the second flow path to transport fluid in both directions, and a lower valve installed between the detection module and the second pump.

[0012] An automatic measuring device having an automatic quantitative function for a liquid sample according to one aspect of the present invention may further include a third air supply pipe connected to the second flow path to supply air to the detection module, a second discharge flow path connected to the second flow path to discharge waste liquid, and a second discharge valve controlling the connection between the third air supply pipe and the second discharge flow path and the second flow path.

[0013] An automatic analysis method for analyzing a sample using an automatic measuring device having an automatic quantitative function for a liquid sample, comprising: a main manifold having a plurality of inlets for introducing a reagent and a diluent according to one aspect of the present invention; a detection module connected to the main manifold for reacting the reagent and the sample and detecting a substance of interest; a first flow path connecting the main manifold and the detection module; and a first pump installed in the first flow path for moving the reagent and the sample, comprises: a sample or standard solution injection step of injecting a sample or standard solution into the detection module through the first flow path; a diluent injection step of injecting a diluent into the detection module using the main manifold; a reagent injection step of supplying a reagent to the detection module using the main manifold; and a detection step of detecting a substance of interest contained in the sample in the detection module, wherein the sample or standard solution injection step comprises a sample filling step of filling the first flow path with a sample using the first pump; and opening a first air valve installed in the first flow path to inject air through the first air valve and downstream of the first air valve It may include an excess liquid discharge step for discharging a sample located therein, and a quantitative liquid supply step for opening a quantitative control unit positioned upstream of the first air valve in the first flow path and injecting air through the quantitative control unit to supply a sample located between the quantitative control unit and the first air valve to the detection module.

[0014] According to one aspect of the present invention, the diluent injection step may include a diluent filling step of opening a solenoid valve installed at the diluent inlet of the main manifold and filling the diluent into the first flow path using the first pump; an excess diluent discharge step of opening a second air valve disposed between the first air valve and the first pump in the first flow path and injecting air through the second air valve to discharge the diluent located downstream of the second air valve; and a quantitative diluent supply step of opening the first air valve and injecting air through the first air valve to supply the diluent located between the first air valve and the second air valve to the detection module. Effects of the invention

[0015] As described above, according to the present invention, since quantitative injection of standard solution and sample is possible through the length of the flow path and air injection, a sample of the same volume as the standard solution can be injected into the detection module regardless of the distance between the tank where the sample is stored and the automatic measuring device, thereby increasing the accuracy and precision of the analysis.

[0016] In addition, the supply volume of the standard solution, sample, and diluent can be determined by the length of the flow path connected between the two valves into which air is injected. Therefore, the supply volume of the standard solution, sample, and diluent can be easily changed by changing the length of the aforementioned flow path.

[0017] In addition, since it is possible to inject a quantitative amount of sample and diluent even with changes in the pump's flow rate, high accuracy and precision can be maintained for a long period without frequent calibration.

[0018] In addition, an interference filter with a narrow full width at half maximum in the detection module can minimize the reduction of the detection concentration range caused by ambient light or multicolored wavelengths entering the detector.

[0019] In addition, multiple interference filters with different wavelengths can be automatically controlled, enabling the analysis of multiple substances of interest.

[0020] In addition, through the automatic injection of a quantitative sample with high precision and a diluent, an accurate calibration curve can be automatically obtained using only one high-concentration standard solution. Therefore, self-verification and calibration of the analytical performance of the automatic measuring device are possible through periodic automatic analysis of the standard solution.

[0021] In addition, the amount of cleaning liquid used and the amount of waste liquid discharged can be minimized by separating the functions of each pump—such as the first pump injecting liquid into the detection module, the second pump discharging waste liquid, and mixing liquid by air injection—and improving cleaning efficiency through the unidirectional transfer of liquid.

[0022] In addition, the measurement cycle can be shortened as the first flow path can be cleaned through the first pump during liquid mixing and signal detection in the detection module. Brief explanation of the drawing

[0023] FIG. 1 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a first embodiment of the present invention. FIG. 2 is a top view of an interference filter according to a first embodiment of the present invention. FIG. 3 is a configuration diagram illustrating an interference filter section according to a first embodiment of the present invention. Figure 4 is a diagram showing the state in which the interference filter has moved in the interference filter section of Figure 2. FIG. 5 is a drawing illustrating an interference filter section according to a modified example of the first embodiment of the present invention. FIG. 6 is a flowchart for explaining an automatic analysis method according to a first embodiment of the present invention. FIG. 7 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a second embodiment of the present invention. FIG. 8 is a flowchart for explaining an automatic analysis method according to a second embodiment of the present invention. FIG. 9 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a third embodiment of the present invention. FIG. 10 is a perspective view illustrating a quantitative manifold according to a third embodiment of the present invention. Figure 11 is a cross-sectional view taken along the line A1-A1 in Figure 10. FIG. 12 is a flowchart for explaining an automatic analysis method according to a third embodiment of the present invention. Specific details for implementing the invention

[0024] The present invention is capable of various modifications and may have various embodiments, and specific embodiments are illustrated and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and it should be understood that it includes all modifications, equivalents, and substitutions that fall within the spirit and scope of the invention.

[0025] The terms used in this invention are used merely to describe specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this invention, terms such as "comprising" or "having" are intended to indicate the existence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0026] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Note that in the accompanying drawings, identical components are indicated by the same reference numerals whenever possible. Furthermore, detailed descriptions of known functions and configurations that may obscure the essence of the present invention will be omitted. For the same reason, some components in the accompanying drawings may be exaggerated, omitted, or schematically depicted.

[0027] Hereinafter, an automatic measuring device having an automatic quantitative function for a liquid sample according to one embodiment of the present invention will be described.

[0028] FIG. 1 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a first embodiment of the present invention.

[0029] Referring to FIG. 1, an automatic measuring device (101) having an automatic quantitative function for a liquid sample according to the present embodiment may include a main manifold (110), a first pump (121), a second pump (123), a sample flow path (145), a detection module (130), a first flow path (141), a second flow path (142), a first discharge flow path (146), and a second discharge flow path (147), a first air valve (151), a second air valve (152), a first discharge control valve (153), a second discharge control valve (155), and a heater (165).

[0030] The main manifold (110) controls the flow of fluids such as reagents, diluents, and air. The main manifold (110) is equipped with multiple inlets for the introduction of reagents, and a solenoid valve (114) for controlling the flow of fluid may be installed at each inlet. The main manifold (110) has an internal passage (112) that extends in a straight line, and multiple inlets are connected to the internal passage (112).

[0031] In the main manifold (110), a conveying air inlet (176) for air to be introduced, a quantitative air inlet (178), a diluent inlet (116) for diluent to be introduced, a reagent inlet (117) for various reagents to be introduced, and a standard solution inlet (118) for standard solution to be introduced may be formed. Additionally, a sample flow path (145) for supplying a sample may be connected to the main manifold (110).

[0032] However, the present invention is not limited thereto, and the quantitative air inlet (178) and the sample flow path (145) may be positioned outside the main manifold (110) and connected to the first flow path (141).

[0033] Here, the standard solution is a solution in which the concentration of the substance to be detected (hereinafter referred to as the substance of interest) is accurately known, and is used to obtain a calibration curve that represents the correlation between the detection signal intensity (absorbance) and the concentration of the substance of interest. By substituting the absorbance of the sample into the aforementioned calibration curve, the concentration of the substance of interest can be quantitatively analyzed.

[0034] A transfer control valve (156) may be connected to the transfer air inlet (176), and a metering control unit (157) may be connected to the metering air inlet (178). The transfer control valve (156) controls the air flowing in through the transfer air inlet (176), and the metering control unit (157) controls the air flowing in through the metering air inlet (178).

[0035] A solenoid valve (114) is installed at the dilution solution inlet (116), the reagent inlet (117), and the standard solution inlet (118), and the amount of reagent flowing in can be controlled by the opening time of the solenoid valve (114). The opening time of the solenoid valve (114) can be adjusted to be short, such as 0.001 seconds, and accordingly, the amount of reagent flowing in can be controlled significantly more precisely than in the past. In addition, the flow rate of the liquid can be controlled by a pump.

[0036] Air can be introduced through the transfer air inlet (176) or the metering air inlet (178) by the first pump (121), and negative pressure can be formed by the first pump (121), through which air can be sucked into the main manifold (110). The introduced air can push the liquid to the detection module (130). Accordingly, the supply amount of liquid can be controlled by the air.

[0037] When the part connected to the first flow path (141) is referred to as the front, the transfer air inlet (176) may be located at the rearmost (upstream side) of the main manifold (110), and the metering air inlet (178) may be located at the frontmost (downstream side). Additionally, a diluent inlet (116), a reagent inlet (117), a standard solution inlet (118), and a sample flow path (145) may be connected between the transfer air inlet (176) and the metering air inlet (178). Accordingly, after the liquid is supplied, air can be supplied to the transfer air inlet (176) to push out all the liquid remaining in the internal passage (112) and the first flow path (141).

[0038] The first flow path (141) may include a metering flow path (149) disposed between the metering control unit (157) and the first air valve (151). The metering flow path (149) is a flow path connecting the metering control unit (157) and the first air valve (151), and the amount of liquid supplied can be controlled by the length of the metering flow path (149).

[0039] The first flow path (141) is composed of a tube connecting the main manifold (110) and the detection module (130) and delivers reagents and samples to the detection container (131). The first flow path (141) is connected to the upper part of the detection container (131) to supply samples and reagents to the detection container (131).

[0040] The second Euro (142) connects the detection module (130) and the second discharge control valve (155), and can discharge waste liquid from the detection container (131) or supply air to the bottom of the detection container (131).

[0041] The first pump (121) is connected to the first flow path (141) and can move fluid in both directions. The first pump (121) may be a peristaltic pump, thereby preventing the inside of the first pump (121) from being contaminated by the sample and reagent and allowing for precise control of the amount of sample and reagent to be injected.

[0042] The sample channel (145) is connected to the main manifold (110) and can supply a sample to the first channel (141) through the main manifold (110). The sample channel (145) can be connected to a tank (200) in which a sample is stored to supply the sample. A sample valve (158) for controlling the supply of the sample may be installed between the sample channel (145) and the main manifold (110).

[0043] A first air valve (151) and a second air valve (152) are installed in the first Euro (141), and the first air valve (151) and the second air valve (152) are located between the main manifold (110) and the first pump (121). Additionally, the first air valve (151) and the second air valve (152) may be spaced apart so that liquid can be stored.

[0044] A liquid detection sensor (161, 162) for detecting liquid is placed in the first Euro (141), and the liquid detection sensor (161, 162) can be placed downstream of the first air valve (151) and downstream of the second air valve (152), respectively.

[0045] The first air valve (151) and the second air valve (152) control the volume of the diluent, and the first air supply pipe (171) is connected to the first air valve (151), and the second air supply pipe (172) can be connected to the second air valve (152).

[0046] The volume of the sample can be controlled by the first air valve (151) and the quantitative control unit (157). Specifically, the sample valve (158) installed in the sample flow path (145) can be opened, and the sample can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0047] When the sample is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the sample located downstream of the first air valve (151). Subsequently, the quantitative control unit (157) is opened, and the first air valve (151) and the first discharge control valve (153) are closed so that the sample located between the quantitative control unit (157) and the first air valve (151) can be supplied to the detection module (130). Additionally, the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the sample located between the sample valve (158) and the quantitative control unit (157). The volume of the sample supplied to the detection module (130) can be determined according to the length of the quantitative flow path (149).

[0048] In addition, the volume of the standard solution can also be controlled to be the same as the sample. Specifically, the solenoid valve (114) installed at the standard solution inlet (118) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0049] When the standard solution is filled in the metering path (149) and the first path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the standard solution located downstream of the first air valve (151). Afterwards, the metering control unit (157) is opened and the first air valve (151) and the first discharge control valve (153) are closed so that the standard solution located between the metering control unit (157) and the first air valve (151) can be supplied to the detection module (130). Additionally, the transfer control valve (156) and the first discharge control valve (153) are opened so that all the standard solution located between the solenoid valve (114) installed at the standard solution inlet (118) and the metering control unit (157) can be discharged. The volume of the standard solution supplied to the detection module (130) can be determined according to the length of the quantitative flow path (149).

[0050] The first air valve (151) and the second air valve (152) can control the supply amount of the diluent with high precision, and accordingly, the accuracy and precision of the analysis can be improved. Specifically, the solenoid valve (114) installed at the diluent inlet (116) can be opened and the diluent can be supplied to the first flow path (141) using the first pump (121). When the diluent fills the first flow path (141), the second air valve (152) and the first discharge control valve (153) are opened to discharge the diluent located downstream of the second air valve (152). Afterwards, the first air valve (151) is opened and the second air valve (152) and the first discharge control valve (153) are closed to supply the diluent located between the first air valve (151) and the second air valve (152) to the detection module (130). In addition, the transfer control valve (156) and the first discharge control valve (153) can be opened to discharge all the diluted liquid located between the solenoid valve (114) installed at the diluted liquid inlet (116) and the first air valve (151).

[0051] However, the present invention is not limited thereto, and the second air valve (152) may be omitted, and the supply amount of the diluent may be controlled by the first pump (121). By controlling the operating time of the first pump (121), the volume of the diluent supplied to the detection module (130) can be controlled.

[0052] <Preparation of Multiple Standard Solutions for Calibration Curve Construction Using High-Concentration Standard Solution and Diluent>

[0053] As described above, the volume of liquid supplied to the detection module (130) can be determined by the length of the flow path. By utilizing this characteristic, standard solutions of various concentrations can be prepared by mixing a standard solution containing a high concentration of the substance of interest with a diluent.

[0054] To prepare a calibration curve, standard solutions having concentrations of 0, 10, 90, and 100 ppm are prepared using a standard solution containing 100 ppm of the substance of interest and a diluent. To do this, the length of the first flow path (141) between the first air valve (151) and the second air valve (152) is configured to be nine times the length of the quantitative flow path (149). Here, it is assumed that the inner diameters of the first flow path (141) and the quantitative flow path (149) are the same.

[0055] In the following description, the standard solution supplied to the quantitative path (149) and the first path (141) through the standard solution inlet (118) is defined as a standard solution containing 100 ppm of the substance of interest, and the diluent supplied through the diluent inlet (116) is considered to be a 0 ppm standard solution that does not contain the substance of interest.

[0056] Preparation of <0 ppm Standard Solution>

[0057] The solenoid valve (114) installed at the dilution inlet (116) can be opened, and the dilution can be supplied to the metering path (149) and the first path (141) using the first pump (121). When the standard solution is filled in the metering path (149) and the first path (141), the second air valve (152) and the first discharge control valve (153) can be opened to discharge the dilution located downstream of the second air valve (152), and the metering control unit (157) can be opened to supply the dilution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) can be opened to discharge all the dilution between the solenoid valve (114) installed at the dilution inlet (116) and the metering control unit (157).

[0058] <Preparation of 10 ppm standard solution>

[0059] 1. The standard solution is measured through the quantitative flow path (149).

[0060] The solenoid valve (114) installed at the standard solution inlet (118) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0061] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the standard solution located downstream of the first air valve (151), the quantitative control unit (157) is opened to supply the standard solution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the standard solution located between the solenoid valve (114) installed in the standard solution inlet (118) and the quantitative control unit (157).

[0062] 2. The diluted solution is measured through the first flow path (141) between the first air valve and the second air valve.

[0063] The solenoid valve (114) installed at the dilution inlet (116) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0064] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the second air valve (152) and the first discharge control valve (153) are opened to discharge the diluted solution located downstream of the second air valve (152), the first air valve (151) is opened to supply the diluted solution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the diluted solution between the solenoid valve (114) installed at the diluted solution inlet (116) and the first air valve (151).

[0065] <Preparation of 90 ppm standard solution>

[0066] 1. A standard solution is measured through the first flow path (141) between the first air valve and the second air valve.

[0067] The solenoid valve (114) installed at the standard solution inlet (118) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0068] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the second air valve (152) and the first discharge control valve (153) are opened to discharge the standard solution located downstream of the second air valve (152), the first air valve (151) is opened to supply the standard solution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the standard solution between the solenoid valve (114) installed in the standard solution inlet (118) and the first air valve (151).

[0069] 2. Measure the diluted solution into the quantitative flow path (149).

[0070] The solenoid valve (114) installed at the dilution inlet (116) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0071] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the diluted solution located downstream of the first air valve (151), the quantitative control unit (157) is opened to supply the diluted solution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the diluted solution between the solenoid valve (114) installed in the diluted solution inlet (116) and the quantitative control unit (157).

[0072] <Preparation of 100 ppm standard solution>

[0073] The solenoid valve (114) installed at the standard solution inlet (118) can be opened, and the standard solution can be supplied to the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0074] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the second air valve (152) and the first discharge control valve (153) are opened to discharge the standard solution located downstream of the second air valve (152), and the quantitative control unit (157) is opened to supply the standard solution to the detection module (130), and the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the diluted solution between the solenoid valve (114) installed in the standard solution inlet (118) and the quantitative control unit (157).

[0075] A calibration curve can be derived by supplying standard solutions of 0 ppm, 10 ppm, 90 ppm, and 100 ppm to the detection container (131) in sequence and measuring them.

[0076] A first discharge path (146) is connected to the first path (141) via a first discharge control valve (153). The first discharge path (146) is connected to the first path (141) between the first pump (121) and the heater (165) and discharges excess sample and excess diluted liquid. The first discharge control valve (153) controls the connection between the first discharge path (146) and the first path (141) and may be a three-way valve.

[0077] Here, the excess sample includes the sample analyzed immediately prior that remains in the sample flow path (145) during the process of analyzing the next sample. By discharging it through the first discharge flow path (146), the analysis error caused by interference from the sample analyzed immediately prior can be minimized.

[0078] A heater (165) is installed between the first pump (121) and the detection container (131), and the heater (165) is connected to the first flow path (141) to heat the liquid flowing into the detection container (131). The heater (165) may be a continuous flow type heating device and can heat the liquid while it is moving without stopping. By heating the liquid to a preset reference temperature and supplying it to the detection container (131), the heater (165) maintains a constant reaction temperature, thereby minimizing analysis errors caused by ambient temperature changes. Here, the reference temperature may vary depending on the substance of interest, reaction, detection concentration range, etc.

[0079] The detection container (131) is connected to the first flow path (141) to receive the reagent and sample from the first flow path (141), and the reagent and sample can be mixed within the detection container (131). The detection module (130) is a device for the reaction between the reagent and the sample and for the detection of the substance of interest.

[0080] The detection module (130) may include a detection container (131), a light source (132) that irradiates light, a detector (133) capable of measuring the intensity of light, and an interference filter (201) capable of selectively transmitting only light of a specific wavelength range.

[0081] FIG. 2 is a top view of an interference filter according to a first embodiment of the present invention, FIG. 3 is a configuration diagram showing an interference filter section according to a first embodiment of the present invention, and FIG. 4 is a diagram showing the state in which the interference filter has moved from the interference filter section of FIG. 2.

[0082] Referring to FIGS. 2 to 4, the interference filter section (201) may include a plurality of interference filters (220), a holder (210), a filter transfer section (230), and a linear guide (250).

[0083] The interference filter section (201) includes a plurality of interference filters (220), and the interference filters (220) may be made of a material that selectively transmits light of different wavelength ranges. The interference filter (220) can selectively transmit only the wavelength range with a narrow full width at half maximum among the multicolor wavelengths of the light source (132).

[0084] The interference filter (220) may be an optical filter that selectively transmits only a narrow half-width wavelength band corresponding to the maximum absorption wavelength of the substance of interest or the color-developing solution. The interference filter (220) may be placed between the light source (132) and the detector (133), or between the light source (132) and the detection container (131) or between the detection container (131) and the detector (133).

[0085] A holder (210) supports a plurality of interference filters (220). A plurality of slits (212) through which light passes may be formed in the holder (210), and the interference filters (220) may be installed to cover the slits (212). A linear guide (250) may be made of a rail that supports the movement of the holder (210). The holder (210) may include a slide (215) that is coupled to the linear guide (250) and moves along the linear guide (250), and a frame (216) coupled to the slide (215).

[0086] The filter transfer unit (230) is combined with the holder (210) to move the holder (210) and may be composed of a pneumatic actuator, etc. The filter transfer unit (230) controls the selection of one interference filter (220) among a plurality of interference filters (220) with different wavelengths of transmitted light for the analysis of a plurality of substances of interest.

[0087] As shown in FIG. 5, the holder (210) is formed in the shape of a disc, and the filter transfer unit (230) may be formed by a motor that rotates the holder (210).

[0088] The detection container (131) may be formed in the shape of a tube that accommodates a sample and a reagent. The light source (132) may be an LED, a light bulb, etc. The detector (133) may be an image detector or a photodiode, etc. The detector (133) is spaced apart from the light source (132) with the detection container (131) in between.

[0089] A second flow path (142) may be connected to the detection container (131) to discharge waste liquid containing used samples and reagents or to supply air to the detection container (131). A second pump (123) is installed in the second flow path (142), and the second pump (123) may discharge liquid from the detection container (131) or supply air to the detection container (131). The second pump (123) may be composed of various pumps that transport fluid in both directions.

[0090] Additionally, a lower valve (154) is installed in the second flow path (142), and the lower valve (154) may be located between the second pump (123) and the detection container (131). A third air supply pipe (173) that supplies air via a second discharge control valve (155) and a second discharge flow path (147) that discharges waste liquid may be connected to the second flow path (142).

[0091] When the second discharge control valve (155) is connected to the third air supply pipe (173) and the second flow path (142), and the second pump (123) is operated to suck air, air can be supplied to the bottom of the detection container (131) through the third air supply pipe (173). At this time, the air supplied to the bottom of the detection container (131) can mix with a liquid (sample, standard solution, diluent, reagent).

[0092] Meanwhile, when the second discharge control valve (155) is connected to the second discharge path (142) and the second discharge path (147), and the second pump (123) is operated to discharge, the waste liquid used for detection can be discharged through the second discharge path (147).

[0093] As described above, according to the present embodiment, the volume of the liquid can be accurately controlled so that the sample and diluent can be supplied to the detection container (131), thereby improving the accuracy and precision of the analysis. In addition, a heater is installed in the first flow path (141) to maintain a constant reaction temperature, thereby minimizing analysis errors caused by ambient temperature changes.

[0094] Below, an automatic analysis method according to the first embodiment of the present invention will be described.

[0095] FIG. 6 is a flowchart for explaining an automatic analysis method according to a first embodiment of the present invention.

[0096] Referring to FIGS. 1 to 6, the automatic analysis method according to the present embodiment may include a sample or standard solution injection step (S101), a diluent injection step (S102), a reagent injection step (S103), a mixing step (S104), a detection step (S105), a waste liquid discharge step (S106), and a washing step (S107).

[0097] The sample or standard solution injection step (S101) injects a sample or standard solution into a detection container (131) through a sample channel (145) and a first channel (141).

[0098] The sample or standard solution injection step (S101) may include a sample filling step in which a sample valve (158) is opened and a sample is filled into the quantitative path (149) and the first path (141) using a first pump (121), or a standard solution filling step in which a solenoid valve (114) installed at the standard solution inlet (118) is opened and a standard solution is supplied into the quantitative path (149) and the first path (141) using a first pump (121).

[0099] In the sample or standard solution injection step (S101), if the standard solution is injected into the detection container (131), a calibration curve is derived using the standard solution, and if the sample is injected into the detection container (131), the concentration of the substance of interest can be quantitatively analyzed using the calibration curve.

[0100] The automatic analysis method according to the present embodiment can quantitatively analyze the concentration of a substance of interest by supplying a standard solution to a detection container (131) to derive a calibration curve, and then using a sample in the detection container.

[0101] Additionally, the sample or standard solution injection step (S101) comprises: an excess liquid discharge step in which, when the sample or standard solution is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to inject air into the first flow path (141) through the first air valve (151) and discharge the sample or standard solution located downstream of the first air valve (151); a quantitative liquid supply step in which the quantitative control unit (157) is opened and the first air valve (151) and the first discharge control valve (153) are closed to inject air into the first flow path (141) through the quantitative control unit (157) and supply the sample or standard solution located between the quantitative control unit (157) and the first air valve (151) to the detection container (131); and the transfer control valve (156) and the first discharge control valve (153). It may include a step of opening and discharging the liquid located upstream of the quantitative control unit (157). Additionally, the sample or standard solution injection step (S101) may heat the liquid to a preset reference temperature using a heater (165) and supply it to the detection container (131).

[0102] Meanwhile, the diluent injection step (S102) injects the diluent into the detection container (131) using the main manifold (110). The dilution injection step (S102) comprises a dilution filling step in which a solenoid valve (114) installed at the dilution inlet (116) is opened and the dilution is filled into the first flow path (141) using the first pump (121); an excess dilution discharge step in which, when the dilution is filled into the first flow path (141), the second air valve (152) and the first discharge control valve (153) are opened to inject air into the first flow path (141) through the second air valve (152) and discharge the dilution located downstream of the second air valve (152); a quantitative dilution supply step in which the first air valve (151) is opened and the second air valve (152) and the first discharge control valve (153) are closed to supply the dilution located between the first air valve (151) and the second air valve (152) to a detection container (131); a transfer control valve (156) and the first discharge The method may include a step of opening the control valve (153) to discharge the diluent located between the solenoid valve (114) installed at the diluent inlet (116) and the first air valve (151). Additionally, the diluent injection step (S102) may control the volume of the diluent supplied to the detection module (130) by controlling the operating time of the first pump (121).

[0103] The reagent injection step (S103) supplies a reagent to the detection container (131) using the main manifold (110). Here, the reagent may consist of a reducing agent, an oxidizing agent, a colorimetric agent, an acidic solution, a basic solution, etc. The reagent injection step (S103) may include a step of supplying a quantitative reagent by controlling the opening time of a solenoid valve (114) that controls the movement of the reagent, and a reagent transfer step of moving the reagent to the detection container (131) by opening a transfer control valve (156) and injecting air into the first flow path (141).

[0104] The mixing step (S104) mixes the sample or standard solution and the reagent by injecting air into the detection container (131). The mixing step (S104) mixes the sample, standard solution, diluent, or reagent by connecting the third air supply pipe (173) and the second flow path (142) using the second discharge control valve (155) and supplying air to the detection container (131) through the third air supply pipe (173) and the second flow path (142).

[0105] The detection step (S105) detects a substance of interest contained in a sample or standard solution using a light source (132) and a detector (133). The detection step (S105) can detect a substance of interest contained in a sample by irradiating light onto a detection container (131) in which a liquid is stored and measuring the intensity of the light. The liquid stored in the detection container (131) may contain a sample and a reagent, and the reagent may consist of a color-developing solution.

[0106] Additionally, the detection step (S105) can control the interference filter (220) by moving it so that one of the multiple interference filters (220) is positioned between the light source (132) and the detector (133).

[0107] The waste liquid discharge step (S106) uses a second discharge control valve (155) to connect the second flow path (142) to the second discharge flow path (147) and discharges the waste liquid, which is a mixture of a sample and a reagent stored in the detection container (131), into the second discharge flow path (147).

[0108] The washing step (S107) washes the detection container (131). The washing step (S107) supplies a diluent to the detection container (131), supplies air to the detection container (131) where the diluent is stored to form bubbles to wash the detection container (131), and can discharge the diluent used for washing through the second flow path (142) and the second discharge flow path (147).

[0109] The method may include the steps of opening a solenoid valve (114) to supply a diluent to the first path (141), opening a transfer control valve (156) to inject air into the first path (141) to move the diluent to the detection container (131), connecting the third air supply pipe (173) and the second path (142) using the second discharge control valve (155) to supply air to the detection container (131) through the second path (142) to form bubbles and clean the detection container (131), and connecting the second path (142) to the second discharge path (147) using the second discharge control valve (155) to discharge the diluent used for cleaning from the detection container (131) to the second discharge path (147).

[0110] Additionally, the washing step (S107) may further include the step of opening the solenoid valve (114) to supply the diluent to the first flow path (141) and opening the transfer control valve (156) to inject air into the first flow path (141) to move the diluent to the detection container (131) and fill the inside of the detection container (131) with the diluent.

[0111] Additionally, the cleaning step (S107) can control the interference filter (220) by moving it so that one of the interference filters (220) is positioned between the light source (132) and the detector (133).

[0112] The standard solution injection step (S101) to the washing step (S107) are repeated so that a calibration curve using the standard solution can be derived and the concentration of the substance of interest can be quantitatively analyzed from the detection signal of the sample.

[0113] Hereinafter, an automatic measuring device having an automatic quantitative function for a liquid sample according to a second embodiment of the present invention will be described.

[0114] FIG. 7 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a second embodiment of the present invention.

[0115] Referring to FIG. 7, the automatic measuring device (102) having an automatic quantitative function for a liquid sample according to the present embodiment is structured identically to the automatic measuring device having an automatic quantitative function for a liquid sample according to the first embodiment, except for the main manifold (110), auxiliary manifold (120), transfer air inlet (176), and sample flow path (145), so a redundant description of the same configuration is omitted.

[0116] In the main manifold (110), a conveying air inlet (176), a diluent inlet (116), a reagent inlet (117), and a standard solution inlet (118) are formed, and the quantitative air inlet (178) and the sample flow path (145) are connected to the first flow path (141).

[0117] The sample flow path (145) is connected to the upstream side of the quantitative air inlet (178) and can be connected to the first flow path (141) via the sample valve (158). The sample flow path (145) connects the water tank (200) where the sample is stored with the first flow path (141). The quantitative air inlet (178) can be connected to the first flow path via the quantitative control unit (157). The first flow path (141) may include a quantitative flow path (149) formed between the quantitative control unit (157) and the first air valve (151).

[0118] An auxiliary manifold (120) may be connected to the second air valve (152). The auxiliary manifold (120) controls the flow of fluids such as reagents, diluents, and air. The auxiliary manifold (120) is provided with a plurality of inlets (126) for the introduction of reagents, and a solenoid valve (124) for controlling the flow of fluid may be installed at each inlet. An internal passage (122) that extends in a straight line is formed in the auxiliary manifold (120), and a plurality of inlets (126) are connected to the internal passage (122).

[0119] Reagents, air, and diluents can be supplied to the detection module (130) through the auxiliary manifold (120). Accordingly, when a large number of reagents are required, the reagents can be supplied quickly, and the time for injecting the diluent that acts as a cleaning solution can be shortened. Accordingly, the measurement cycle can be shortened.

[0120] The first air valve (151) is connected to the first air supply pipe (171) and the first flow path (141), and the first air valve (151) is located downstream of the quantitative control unit (157). The quantitative control unit (157) and the first air valve (151) are spaced apart, and the quantitative control unit (157) and the first air valve (151) may be spaced apart by the volume of the sample injected into the detection container (131), and the quantitative flow path (149) is located between the quantitative control unit (157) and the first air valve (151). Only the sample located at the quantitative control unit (157) and the first air valve (151) is supplied to the detection container (131).

[0121] That is, when the sample valve (158) is opened and the sample is supplied to the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the sample located between the first air valve (151) and the detection container (131) through the first discharge flow path (146), and the quantitative control unit (157) is opened to move the sample located between the quantitative control unit (157) and the first air valve (151) to the detection container (131). In addition, the transfer control valve (156) and the first discharge control valve (153) are opened to discharge all the sample located between the sample valve (158) and the quantitative control unit (157).

[0122] Below, an automatic analysis method according to a second embodiment of the present invention will be described.

[0123] FIG. 8 is a flowchart for explaining an automatic analysis method according to a second embodiment of the present invention.

[0124] Referring to FIGS. 7 and 8, the automatic analysis method according to the present embodiment may include a sample or standard solution injection step (S201), a diluent injection step (S202), a reagent injection step (S203), a mixing step (S204), a detection step (S205), a waste liquid discharge step (S206), and a washing step (S207).

[0125] The automatic analysis method according to the present embodiment is composed of the same process as the automatic analysis method according to the first embodiment described above, except for the sample or standard solution injection step (S201), so a redundant description of the same process is omitted.

[0126] The sample or standard solution injection step (S201) injects a sample or standard solution into a detection container (131) through a sample channel (145), a quantitative channel (149), and a first channel (141).

[0127] The sample or standard solution injection step (S201) may include a sample filling step in which the sample valve (158) is opened and the sample is filled into the quantitative flow path (149) and the first flow path (141) using the first pump (121), or a standard solution filling step in which the solenoid valve (114) installed at the standard solution inlet (118) is opened and the standard solution is supplied into the quantitative flow path (149) and the first flow path (141) using the first pump (121).

[0128] The method may include an excess liquid discharge step in which, when a sample or standard solution is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the sample or standard solution located downstream of the first air valve (151); a quantitative liquid supply step in which the quantitative control unit (157) is opened and the first air valve (151) and the first discharge control valve (153) are closed to supply the sample or standard solution located between the quantitative control unit (157) and the first air valve (151) to the detection container (131); and a residual liquid discharge step in which the transfer control valve (156) and the first discharge control valve (153) are opened to discharge the sample or standard solution located upstream of the quantitative control unit (157). The sample or standard solution injection step (S201) is performed outside the main manifold (110).

[0130] Hereinafter, an automatic measuring device having an automatic quantitative function for a liquid sample according to the third embodiment of the present invention will be described.

[0131] FIG. 9 is a configuration diagram illustrating an automatic measuring device having an automatic quantitative function for a liquid sample according to a third embodiment of the present invention, FIG. 10 is a perspective view illustrating a quantitative manifold according to a third embodiment of the present invention, and FIG. 11 is a cross-sectional view taken along line A1-A1 in FIG. 10.

[0132] Referring to FIGS. 9 to 11, the automatic measuring device (103) having an automatic quantitative function for a liquid sample according to the present embodiment is structured identically to the automatic measuring device having an automatic quantitative function for a liquid sample according to the first embodiment, except for the main manifold (110), the quantitative manifold (180), and the sample flow path (145), so a redundant description of the same configuration is omitted.

[0133] The main manifold (110) is formed with a transport air inlet (176) through which air is introduced, a diluent inlet (116), a reagent inlet (117), and a standard solution inlet (118). The main manifold (110) can be connected to a quantitative manifold (180) via an intermediate flow path (119).

[0134] The sample flow path (145) is connected to the upstream side of the quantitative manifold (180), and can be connected via a sample valve (158). The sample flow path (145) is connected to a tank (200) in which the sample is stored to supply the sample. When the sample valve (158) is opened, the sample can be supplied to the first flow path (141) through the sample flow path (145).

[0135] The metering manifold (180) may include a metering air inlet (186), a third air inlet (187), a metering control unit (181), and an intermediate control valve (182). The metering control unit (181) may be connected to the metering air inlet (186), and the intermediate control valve (182) may be connected to the third air inlet (187).

[0136] The first air valve (151) is located downstream of the quantitative control unit (181). The quantitative control unit (181) and the first air valve (151) are spaced apart, and the quantitative control unit (181) and the first air valve (151) may be spaced apart by the volume of the sample injected into the detection container (131), and a quantitative flow path (149) is located between the quantitative control unit (181) and the first air valve (151). Only the sample located at the quantitative control unit (181) and the first air valve (151) is supplied to the detection container (131).

[0137] The quantitative manifold (180) includes a quantitative manifold body (185) in which a passage for the movement of a sample and air is formed, a quantitative control unit (181) coupled to the quantitative manifold body (185), and an intermediate control valve (182).

[0138] In the quantitative manifold body (185), a sample passage (183) through which a sample moves, an intermediate passage (184) through which fluid delivered from the main manifold (110) moves, a first air supply passage (185a) for supplying air to the quantitative control unit (181), a first air delivery passage (185b) for supplying air from the quantitative control unit (181) to the sample passage (183), a second air supply passage (185c) for supplying air to the intermediate control valve (182), and a second air delivery passage (185d) for supplying air from the intermediate control valve (182) to the sample passage (183) are formed. In the sample passage (183) and the intermediate passage (184), coupling grooves (185e, 185f, 185g) for coupling tubes may be connected.

[0139] The first air supply passage (185a) and the first air delivery passage (185b) are connected via a quantitative control unit (181), and the quantitative control unit (181) can connect or block the first air supply passage (185a) and the first air delivery passage (185b) according to an input signal.

[0140] The second air supply passage (185c) and the second air delivery passage (185d) are connected via an intermediate control valve (182), and the intermediate control valve (182) can connect or block the second air supply passage (185c) and the second air delivery passage (185d) according to an input signal.

[0141] The intermediate passage (184) is connected to the sample passage (183) between the first air transfer passage (185b) and the second air transfer passage (185d), and when air is supplied from the second air transfer passage (185d) by the intermediate control valve (182), the fluid introduced through the intermediate passage (184) can move.

[0142] The volume of the sample can be controlled by the first air valve (151) and the quantitative control unit (181). Specifically, the sample valve (158) installed in the sample path (145) can be opened, and the sample can be supplied to the quantitative path (149) and the first path (141) using the first pump (121). When the sample is filled in the quantitative path (149) and the first path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the sample located downstream of the first air valve (151). Afterward, the quantitative control unit (181) is opened, and the first air valve (151) and the first discharge control valve (153) are closed to supply the sample located between the quantitative control unit (181) and the first air valve (151) to the detection container (131). In addition, the intermediate control valve (182) can be opened to discharge all the samples located between the intermediate control valve (182) and the metering manifold (180).

[0143] Meanwhile, the standard solution can be supplied to the quantitative manifold (180) through the intermediate flow path (119) by means of a solenoid valve (114) installed at the standard solution inlet (118) of the main manifold (110), and can be supplied again from the quantitative manifold (180) to the quantitative flow path (149).

[0144] When the standard solution is filled in the quantitative flow path (149) and the first flow path (141), the first air valve (151) and the first discharge control valve (153) are opened to discharge the standard solution located downstream of the first air valve (151). Afterwards, the quantitative control unit (181) is opened and the first air valve (151) and the first discharge control valve (153) are closed so that the standard solution located between the quantitative control unit (181) and the first air valve (151) can be supplied to the detection container (131).

[0145] In addition, the amount of diluent supplied can be controlled with high precision by filling the space between the first air valve (151) and the second air valve (152), and accordingly, the accuracy and precision of the analysis can be improved.

[0146] In the case of a conventional three-way valve, a problem may occur where the flow path is blocked by particles contained in the sample. However, according to the present embodiment, the sample is not introduced into the quantitative control unit (181) and the intermediate control valve (182), and the supply of air is controlled by the quantitative control unit (181) and the intermediate control valve (182), and the sample is moved by the air, so the malfunction of the quantitative control unit (181) and the intermediate control valve (182) can be reliably prevented.

[0147] Below, an automatic analysis method according to the third embodiment of the present invention will be described.

[0148] FIG. 12 is a flowchart for explaining an automatic analysis method according to a third embodiment of the present invention.

[0149] Referring to FIGS. 9 to 12, the automatic analysis method according to the present embodiment may include a sample or standard solution injection step (S301), a diluent injection step (S302), a reagent injection step (S303), a mixing step (S304), a detection step (S305), a waste liquid discharge step (S306), and a washing step (S307).

[0150] The automatic analysis method according to the present embodiment is composed of the same process as the automatic analysis method according to the first embodiment described above, except for the sample or standard solution injection step (S301), so a redundant description of the same process is omitted.

[0151] The sample or standard solution injection step (S301) injects a sample or standard solution into a detection container (131) through a sample channel (145), a quantitative channel (149), and a first channel (141). The sample or standard solution injection step (S301) may include a sample filling step in which a sample valve (158) is opened and a sample is filled into the first channel (141) using a first pump (121), or a standard solution filling step in which a solenoid valve (114) installed at the standard solution inlet (118) is opened and a standard solution is supplied to the quantitative channel (149) using a first pump (121).

[0152] When a sample or standard solution is filled in the quantitative flow path (149), the first air valve (151) and the first discharge control valve (153) are opened to discharge the sample or standard solution located downstream of the first air valve (151), the quantitative liquid supply step is closed, the first air valve (151) and the first discharge control valve (153) are closed, and air is injected using the quantitative control unit (181) to supply the sample or standard solution located between the quantitative control unit (181) and the first air valve (151) to the detection container (131), and the residual liquid discharge step is opened, the intermediate control valve (182) is opened to discharge the sample or standard solution located between the intermediate control valve (182) and the quantitative manifold (180).

[0153] Although an embodiment of the present invention has been described above, those skilled in the art may modify and change the present invention in various ways by adding, changing, deleting, or adding components, etc., without departing from the spirit of the present invention as described in the claims, and such modifications and changes are also to be included within the scope of the rights of the present invention. Explanation of the symbols

[0154] 101, 102, 103: Automatic measuring device with automatic quantitative function for liquid samples 110: Main manifold 112: Internal passage 114: Solenoid valve 116: Diluent inlet 117: Reagent inlet 118: Standard solution inlet 121: Pump 1 123: Pump 2 130: Detection module 131: Detection container 132: Light source 133: Detector 141: 1st Euro 142: 2nd Euro 145: Sample flow path 149: Quantitative flow path 146: 1st Emission Euro 147: 2nd Emission Euro 151: 1st air valve 152: 2nd air valve 153: 1st discharge control valve 155: 2nd discharge control valve 156: Transfer control valve 157: Metering control unit 165: Heater 176: Transfer air inlet 178, 186: Metering air inlets 180: Metering manifold 181: Metering control unit 182: Intermediate control valve 183: Sample passage 184: Intermediate passage 186: Metering air inlet 187: Third air inlet 200: Water tank 201: Interference filter section 210: Holder 212: Slit 215: Slide 216: Frame 220: Interference filter 230: Filter transfer section 250: Linear Guide

Claims

Claim 1 A main manifold comprising a plurality of inlets for fluid inflow and a valve installed at the inlets to control the movement of the fluid; a detection module connected to the main manifold for the reaction between a reagent and a sample and the detection of a substance of interest; a first flow path connecting the main manifold and the detection module; a first pump installed in the first flow path to transport the fluid; a sample flow path connected to the first flow path or the main manifold to supply a sample; and a first air valve connected to the first flow path to control the supply of air. An automatic measuring device having an automatic quantitative function for a liquid sample, comprising a quantitative control unit connected to the main manifold or the first flow path, spaced apart from the first air valve, and quantifying the volume of a liquid between the first air valve and the first air valve, wherein a first air supply pipe for supplying air is connected to the first air valve, the first air supply pipe is connected to the first flow path via the first air valve, a quantitative air inlet is connected to the quantitative control unit, and the quantitative control unit controls the air flowing in through the quantitative air inlet. Claim 2 An automatic measuring device having an automatic quantitative function for a liquid sample, wherein the first flow path is formed between the quantitative control unit and the first air valve and includes a quantitative flow path for receiving a sample or standard solution. Claim 3 An automatic measuring device having an automatic quantitative function for a liquid sample, wherein, in claim 1, it further comprises a second air valve connected to the first flow path and controlling the supply of air, and the first air valve and the second air valve are spaced apart along the longitudinal direction of the first flow path to accommodate a diluent between the first air valve and the second air valve. Claim 4 An automatic measuring device having an automatic quantitative function for a liquid sample, further comprising an auxiliary manifold connected to the second air valve to control the flow of reagent, diluent, and air in claim 3. Claim 5 An automatic measuring device having an automatic quantitative function for a liquid sample according to claim 1, further comprising a heater disposed between the detection module and the first pump in the first flow path and heating a sample moving along the first flow path, a first discharge flow path connected to the first flow path for discharging excess sample and excess diluted liquid, and a first discharge control valve for controlling the connection between the first discharge flow path and the first flow path. Claim 6 An automatic measuring device having an automatic quantitative function for a liquid sample, wherein, in claim 1, the detection module comprises a light source that irradiates light, a detector that receives light, and an interference filter module disposed between the light source and the detector, and the interference filter module comprises a plurality of interference filters that selectively transmit only light of a preset wavelength range, a holder that supports the interference filters, and a filter transfer unit that moves the holder. Claim 7 An automatic measuring device having an automatic quantitative function for a liquid sample, comprising: a main manifold including a plurality of inlets for fluid inflow and a valve installed in the inlets to control the movement of the fluid; a detection module connected to the main manifold for the reaction between a reagent and a sample and for the detection of a substance of interest; a first flow path connecting the main manifold and the detection module; a first pump installed in the first flow path to transport the fluid; a sample flow path connected to the first flow path or the main manifold to supply a sample; a first air valve connected to the first flow path to control the supply of air; and a quantitative control unit connected to the main manifold or the first flow path, spaced apart from the first air valve to quantify the volume of the liquid between the first air valve and the main manifold, and further comprising a second flow path connected to the detection module to discharge waste liquid from the detection module or supply air to the detection module, a second pump installed in the second flow path to transport fluid in both directions, and a lower valve installed between the detection module and the second pump. Claim 8 An automatic measuring device having an automatic quantitative function for a liquid sample, further comprising, in claim 7, a third air supply pipe connected to the second flow path to supply air to the detection module, a second discharge flow path connected to the second flow path to discharge waste liquid, and a second discharge valve controlling the connection between the third air supply pipe and the second discharge flow path and the second flow path. Claim 9 An automatic analysis method for analyzing a sample using an automatic measuring device having an automatic quantitative function for a liquid sample, comprising: a main manifold having a plurality of inlets for the introduction of a reagent and a diluent; a detection module connected to the main manifold for the reaction between the reagent and the sample and the detection of a substance of interest; a first flow path connecting the main manifold and the detection module; and a first pump installed in the first flow path for moving the reagent and the sample, wherein the method comprises: a sample or standard solution injection step of injecting a sample or standard solution into the detection module through the first flow path; a diluent injection step of injecting a diluent into the detection module using the main manifold; and a reagent injection step of supplying a reagent to the detection module using the main manifold. The automatic analysis method comprises a detection step for detecting a substance of interest contained in a sample in the detection module, wherein the sample or standard solution injection step comprises a sample filling step for filling a sample into the first flow path using the first pump, an excess liquid discharge step for discharging a sample located downstream of the first air valve by opening a first air valve installed in the first flow path and injecting air through the first air valve, and a quantitative liquid supply step for supplying a sample located between the quantitative control unit and the first air valve to the detection module by opening a quantitative control unit spaced upstream of the first air valve in the first flow path and injecting air through the quantitative control unit. Claim 10 In claim 9, the diluent injection step comprises a diluent filling step of opening a solenoid valve installed at the diluent inlet of the main manifold and filling the diluent into the first flow path using the first pump; an excess diluent discharge step of opening a second air valve disposed between the first air valve and the first pump in the first flow path and injecting air through the second air valve to discharge the diluent located downstream of the second air valve; and a quantitative diluent supply step of opening the first air valve and injecting air through the first air valve to supply the diluent located between the first air valve and the second air valve to the detection module.

Images