Antibody Reaction Enhancement and Usage Reduction Device

Abstract

The present invention relates to a device for reducing the use of an antibody solution through antibody reaction enhancement, designed to increase the reactivity of the antibody solution and the blotting membrane and to reduce the use of the antibody solution through a structure including a spreader and a partition designed to ensure uniform distribution of the antibody solution within the reaction vessel. The device comprises: a reaction vessel having an open top and a reaction chamber formed on the inside in the shape of a rounded arch facing downward, so that the antibody solution can be received and a blotting membrane reacting with the antibody solution can be placed therein; and a cylindrical spreader positioned above the blotting membrane within the reaction chamber, which moves by rolling back and forth along the bottom surface of the reaction chamber as the reaction vessel tilts in the front-rear direction.

Description

Technology Field

[0001] The present invention relates to a device for reducing usage through the enhancement of antibody reaction, and more specifically, to a device for reducing usage through the enhancement of antibody reaction designed to reduce the usage of antibody solution by including a spreader and a partition designed to increase the reactivity of the antibody solution and the blotting membrane and to ensure that the antibody solution is uniformly distributed inside the reaction vessel. Background Technology

[0003] Antibody-antigen reactions play a pivotal role in life science and medical research and are a particularly important element in blotting technologies (Western blot, Southern blot, etc.). Generally, blotting analysis proceeds by detecting specific proteins through contact between an antibody solution and a membrane. However, since antibody solutions are expensive reagents, technical improvements are required to reduce unnecessary waste and maximize efficiency.

[0004] In traditional methods, antibody solutions are filled to a specific volume and then brought into contact with a membrane, either manually or by stirring. However, this method may result in an uneven distribution of the solution, which may lead to reduced antibody reactivity or excessive use of the solution.

[0005] To address this, some technologies adopt methods to improve reactivity by circulating the antibody solution or mechanically moving the membrane. However, these methods have the disadvantage of being structurally complex and difficult to maintain.

[0006] Therefore, a technical solution is needed that can maintain uniform contact with the blotting membrane while reducing the amount of antibody solution used. To solve this problem, the present invention enables efficient application and distribution of the antibody solution by utilizing a structural improvement of the reaction chamber and a spreader.

[0007] Meanwhile, the aforementioned background technology is technical information that the inventor possessed for the derivation of the present invention or acquired during the process of deriving the present invention, and it cannot necessarily be considered publicly known technology disclosed to the general public prior to the filing of the present invention. Prior art literature

[0009] Korean Registered Patent No. 10-1090881 (Published Dec. 08, 2011) The problem to be solved

[0010] One aspect of the present invention provides a device for reducing usage through antibody reaction enhancement, having a structure that uniformly distributes the antibody solution within a reaction vessel to improve the reactivity of the antibody solution and the blotting membrane and to reduce the amount of antibody solution used.

[0011] The technical problems of the present invention are not limited to those mentioned above, and other unmentioned technical problems will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0013] A device for reducing usage through improved antibody reaction according to one embodiment of the present invention may include: a reaction vessel having an open top and a reaction chamber formed on the lower side in the shape of a rounded arch so that an antibody solution is received and a blotting membrane that reacts with the antibody solution is disposed therein; and a cylindrical spreader disposed above the blotting membrane within the reaction chamber, which moves by rolling back and forth on the bottom surface of the reaction chamber as the reaction vessel tilts in the front and rear directions.

[0014] In one embodiment, the spreader can rotate and move back and forth along the bottom surface of the reaction chamber to evenly spread the antibody solution applied to it onto the blotting membrane.

[0015] In one embodiment, the reaction vessel may have at least one partition wall for partitioning the internal space of the reaction chamber.

[0016] In one embodiment, the reaction vessel may have at least one protrusion formed at the front and rear ends of the bottom surface of the reaction chamber so as to limit the range of movement of the spreader and the blotting membrane.

[0017] In one embodiment, when the reaction vessel is tilted, the spreader is aligned to prevent it from becoming crooked during movement and to facilitate the smooth movement of the reaction chamber.

[0018] In one embodiment, the reaction vessel may have an ice pack mounting groove formed on the lower side to accommodate an ice pack for a cooling effect.

[0019] In one embodiment, the device for reducing usage through improved antibody reaction according to one embodiment of the present invention may further include a cover seated on the upper side of the reaction vessel to open and close the upper side of the reaction chamber to reduce the evaporation of the antibody solution.

[0020] In one embodiment, the reaction chamber comprises: a chamber space having an open top and a rounded arch shape on the bottom; a receiving pouch made of a thin film capable of expansion and contraction, installed along the lower corners of one side and the other side of the chamber space to cover and position the bottom of the chamber space; a first flat magnetic body made of a soft, rectangular thin film magnetic body attached along the bottom surface of the receiving pouch; a first support plate made of a rectangular flat plate shape formed by rounding in correspondence with the curvature of the bottom surface of the chamber space, with its front end connected and installed above the front end of the chamber space and seated along a portion of the front end of the bottom surface of the chamber space to support the first flat magnetic body; and a second support plate made of a rectangular flat plate shape formed by rounding in correspondence with the curvature of the bottom surface of the chamber space, with its rear end connected and installed above the rear end of the chamber space and seated along a remaining portion of the bottom surface of the chamber space to support the first flat magnetic body. At least one extension formed extending from the rear end of the first support plate facing the second support plate; at least one seating groove formed by recessing from the front end of the second support plate facing the first support plate so that the extension can be positioned; a second flat magnetic body made of a soft thin film magnetic body with opposite polarity to the first support plate and attached along the upper side of the first support plate and the extension to press the receiving pouch against the first support plate; a third flat magnetic body made of a soft thin film magnetic body with opposite polarity to the first support plate and attached along the upper side of the second support plate to press the receiving pouch against the second support plate; and a gear tooth formed along the lower side of the extension.and a drive gear connected to the lower front side of the second support plate to enable rotational driving, which is connected and engaged by gear coupling with the gear teeth, and which rotates in a forward or reverse direction to insert or expose the extension into the seating groove, thereby bringing the first support plate and the second support plate into close contact or separating them to adjust the arrangement angle of the first support plate and the second support plate; may be included.

[0021] In one embodiment, the chamber space may have a gear seating groove formed on the lower side so that the driving gear is positioned therein and can move in the forward and backward directions. Effects of the invention

[0023] According to one aspect of the present invention described above, a reaction chamber inside a reaction vessel is formed in a rounded arch shape, and a partition is placed inside to induce a uniform distribution of the antibody solution. This prevents the solution from being excessively concentrated in a specific area and maximizes the efficiency of the antibody reaction.

[0024] In addition, by using a spreader to uniformly apply the antibody solution onto the blotting membrane, the antibody reaction can be induced to proceed more effectively. This provides the effect of increasing detection efficiency while reducing the amount of antibody solution used.

[0025] By utilizing the structural features of the reaction vessel, protrusions are arranged to restrict the movement of the spreader and the blotting membrane. This allows the spreader to minimize unnecessary movement inside the reaction chamber and maintain consistent contact with the blotting membrane.

[0026] In addition, by maintaining a constant temperature inside the reaction chamber through the ice pack mounting groove, it prevents denaturation of the antibody solution and provides the effect of continuously maintaining optimal reaction conditions.

[0027] By including a cover to conceal the open top of the reaction vessel, the evaporation of the antibody solution and the ingress of external contaminants can be prevented. This maintains the stability of the reaction environment and provides the effect of increased user convenience.

[0028] The effects of the present invention are not limited to those mentioned above, and various effects may be included within the scope obvious to a person skilled in the art from the contents described below. Brief explanation of the drawing

[0030] FIGS. 1 to 5 are drawings illustrating the schematic configuration of a device for reducing usage through antibody reaction enhancement according to one embodiment of the present invention. FIGS. 6 to 9 are drawings showing the detailed configuration of a reaction chamber according to another embodiment of FIG. 1. Specific details for implementing the invention

[0031] The following detailed description of the invention refers to the accompanying drawings, which illustrate specific embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that various embodiments of the invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. It should also be understood that the location or arrangement of individual components within each disclosed embodiment may be changed without departing from the spirit and scope of the invention. Accordingly, the following detailed description is not intended to be limiting, and the scope of the invention is limited only by the appended claims, including all equivalents to those claimed therein, provided appropriately described. Similar reference numerals in the drawings refer to the same or similar functions across various aspects.

[0032] Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the drawings.

[0033] FIGS. 1 to 5 are drawings illustrating the schematic configuration of a device for reducing usage through antibody reaction enhancement according to one embodiment of the present invention.

[0034] Referring to FIGS. 1 to 5, a device (10) for reducing usage through antibody reaction enhancement according to one embodiment of the present invention includes a reaction vessel (200) and a spreader (300).

[0035] The reaction vessel (200) has an open top so that an antibody solution (1) is received and a blotting membrane (2) that reacts with the antibody solution (1) can be placed therein, and a reaction chamber (100) in the shape of a round arch is formed on the lower side.

[0036] Through this, the antibody solution (1) can be smoothly accommodated within the reaction chamber (100), and the blotting membrane (2) can be effectively positioned. Additionally, the solution can be more uniformly distributed inside through the round arch-shaped structure of the reaction chamber (100).

[0037] In one embodiment, the reaction vessel (200) is provided with at least one partition wall (400) for separating the internal space of the reaction chamber (100) in the left and right directions.

[0038] The partition wall (400) formed within the reaction vessel (200) serves to divide the internal space of the reaction chamber (100) into specific regions (100-1, 100-2) separated in the left and right directions. Through this, it is possible to induce multiple membranes (100-1, 100-2) to react with antibodies simultaneously.

[0039] Of course, each membrane (100-1, 100-2) may be individually equipped with independent components (spreader (300-1, 300-2), protrusion (500-1, 500-2), etc.).

[0040] A reaction vessel (200) according to one embodiment of the present invention having the configuration described above can effectively partition the space inside the reaction chamber (100) to maintain a uniform distribution of the antibody solution (1), thereby providing the effect of improving the efficiency of the antibody reaction.

[0041] In one embodiment, the reaction vessel (200) has at least one protrusion (500) formed protruding from the front and rear ends of the bottom surface of the reaction chamber (100) so as to limit the movement range of the spreader (300) and the blotting membrane (2).

[0042] The protrusion (500) prevents unnecessary movement of the spreader (300) within the reaction chamber (100) and serves to maintain the position of the blotting membrane (2). Through this, the spreader (300) can move in a consistent pattern over the blotting membrane (2), and uniform application of the antibody solution (1) becomes possible.

[0043] Additionally, when the reaction vessel (200) is tilted, the spreader (300) is seated to prevent it from moving away from the reaction chamber (100), and at the same time, by balancing the spreader (300), it can help the spreader (300) move uniformly.

[0044] The reaction vessel (200) forms an ice pack mounting groove (700) on the lower side to accommodate an ice pack (3) for a cooling effect.

[0045] The ice pack mounting groove (700) performs the function of maintaining a constant temperature during the antibody reaction process. That is, by inserting the ice pack (3), the temperature of the antibody solution (1) inside the reaction chamber (100) can be lowered and stored, thereby maintaining the stability of the antibody reaction.

[0046] That is, the protein that reacts with the antibody undergoes an antigen-antibody reaction. Since the protein denatures when the temperature rises, inserting an ice pack (3) prevents antigen-antibody denaturation and promotes the reaction.

[0047] A reaction vessel (200) according to one embodiment of the present invention having the configuration described above can effectively restrict the movement of the spreader (300) and the blotting membrane (2) to induce uniform application of the solution, and can prevent the spreader (300) from detaching through the protrusion (500) and minimize the loss of the antibody solution (1). In addition, the reaction environment can be maintained at a constant temperature through the ice pack mounting groove (700), thereby providing the effect of maximizing the efficiency of the antibody reaction.

[0048] The spreader (300) is positioned above the blotting membrane (2) within the reaction chamber (100) and moves by rolling back and forth on the bottom surface of the reaction chamber (100) as the reaction vessel (200) moves 9º in the up and down direction by the stirring device (20) as shown in FIG. 2.

[0049] Through this, the antibody solution (1) can come into more uniform contact with the blotting membrane (2), and the reaction efficiency can be improved. In addition, the fluidity of the solution is increased by the movement of the spreader (300), so that the antibody reaction can proceed more effectively.

[0050] In one embodiment, the spreader (300) moves back and forth rotating along the bottom surface of the reaction chamber (100) and evenly spreads the antibody solution (1) applied to it onto the blotting membrane (2).

[0051] At this time, the spreader (300) moves in the forward and backward directions while rolling along the bottom surface of the reaction chamber (100). Through this, the antibody solution (1) attached to the surface of the spreader (300) can be uniformly distributed over the blotting membrane (2).

[0052] In addition, the antibody solution (1) can be evenly spread across the entire blotting membrane (2) by the rotation of the spreader (300), thereby maximizing the reaction efficiency between the antibody and the blotting membrane (2).

[0053] A spreader (300) according to one embodiment of the present invention having the configuration described above can uniformly apply an antibody solution (1) onto a blotting membrane (2) and provides the effect of improving the efficiency of the antibody reaction while reducing the amount of antibody solution (1) used.

[0054] A device (10) for reducing usage through antibody reaction enhancement according to one embodiment of the present invention having the configuration described above can reduce the amount of antibody solution (1) used while improving reactivity with the blotting membrane (2) and providing the effect of maximizing reaction efficiency.

[0055] A device (10) for reducing usage through antibody reaction enhancement according to one embodiment of the present invention having the configuration described above may further include a cover (800).

[0056] The reaction vessel (200) further includes a cover (800) that is seated on the upper side of the reaction vessel (200) so as to be able to open and close the upper side of the reaction chamber (100).

[0057] The cover (800) serves to cover the open top of the reaction chamber (100) to block contact with the external environment. This prevents the evaporation of the antibody solution (1) inside the reaction chamber (100) and blocks the entry of external contaminants.

[0058] In addition, the cover (800) is designed to be openable and closable, so that the blotting membrane (2) and antibody solution (1) inside the reaction chamber (100) can be easily replaced as needed, and the removal and cleaning of the solution after the reaction are easy.

[0059] A reaction vessel (200) according to one embodiment of the present invention having the configuration described above includes a cover (800), thereby preventing loss of the antibody solution (1), reducing the possibility of contamination, and providing the effect of increasing user convenience in operation.

[0061] FIGS. 6 to 9 are drawings showing the detailed configuration of a reaction chamber according to another embodiment of FIG. 1.

[0062] Referring to FIGS. 6 to 9, a reaction chamber (100a) according to another embodiment includes a chamber space (101), a receiving pouch (102), a first flat magnetic body (103), a first support plate (104), a second support plate (105), an extension (106), a seating groove (107), a second flat magnetic body (108), a third flat magnetic body (109), a gear tooth (110), and a driving gear (111).

[0063] The chamber space (101) is open at the top and formed in a rounded arch shape on the bottom. This allows the antibody solution (1) to be uniformly distributed within the reaction chamber (100).

[0064] In one embodiment, the chamber space (101) is open at the top and formed in a rounded arch shape on the bottom, and a gear seating groove (112) is formed on the bottom so that a driving gear (111) can be positioned and moved in the forward and backward directions.

[0065] The gear seating groove (112) is a structure that allows the driving gear (111) to move smoothly and serves to guide the driving gear (111) to insert or expose the extension part (106) into the seating groove (107) while rotating in the forward or reverse direction. Through this, the arrangement angle of the first support plate (104) and the second support plate (105) can be precisely adjusted.

[0066] The receiving pouch (102) is made of a thin film capable of expansion and contraction and is installed along the lower corners of one side and the other side of the chamber space (101) to cover and position the bottom of the chamber space (101). This allows the antibody solution (1) to be maintained more stably and provides a space for the antibody solution (1) to be received and for the spreader (300) to be seated.

[0067] The first flat magnetic body (103) is made of a soft, square-shaped thin film magnetic body and is attached along the bottom surface of the receiving pouch (102). Through this, the position of the receiving pouch (102) can be stably maintained using magnetism.

[0068] The first support plate (104) is formed in the shape of a square flat plate that is rounded to correspond to the curvature of the bottom surface of the chamber space (101), and is connected and installed on the upper side of the front surface of the chamber space (101) and is seated along a portion of the front surface of the bottom surface of the chamber space (101) to support the first flat magnetic body (103).

[0069] The second support plate (105) is formed in the shape of a square flat plate that is rounded to correspond to the curvature of the bottom surface of the chamber space (101), and its rear end is connected and installed on the upper side of the rear end of the chamber space (101) and is seated along the remaining part of the bottom surface of the chamber space (101) to support the first flat magnetic body (103).

[0070] The extension portion (106) is formed extending from the rear end of the first support plate (104) facing the second support plate (105).

[0071] The seating groove (107) is formed by being recessed from the front end of the second support plate (105) facing the first support plate (104) so ​​that the extension part (106) can be placed therein. Through this, the extension part (106) can be inserted or exposed, thereby allowing the angle of placement of the support plates to be adjusted.

[0072] The second flat magnetic body (108) is made of a soft thin-film magnetic body with opposite polarity to the first support plate (104) and is installed along the upper side of the first support plate (104) and the extension part (106) to bring the receiving pouch (102) into close contact with the first support plate (104).

[0073] The third flat magnetic body (109) is made of a soft thin-film magnetic body with opposite polarity to the first support plate (104) and is attached along the upper side of the second support plate (105) to bring the receiving pouch (102) into close contact with the second support plate (105). Through this, the receiving pouch (102) can be stably maintained and deformation caused by external impact can be minimized.

[0074] The gear teeth (110) are formed along the lower side of the extension (106).

[0075] The drive gear (111) is connected to the lower front side of the second support plate (105) to enable rotational driving, and is connected by engaging with the gear teeth (110) through gear coupling. The drive gear (111) is rotated in a forward or reverse direction to insert or expose the extension part (106) into the seating groove (107), thereby bringing the first support plate (104) and the second support plate (105) into close contact or apart, allowing the positioning angle to be adjusted.

[0076] A reaction chamber (100) according to one embodiment of the present invention having the configuration described above can stably maintain a receiving pouch (102) using magnetism, and can effectively control the placement and movement of the antibody solution (1) by adjusting the angles of the support plates. In addition, it provides the effect of more precisely adjusting the reaction environment through the rotation of the driving gear (111).

[0078] The embodiments described above are for illustrative purposes only, and those skilled in the art will understand that the embodiments described above can be easily modified into other specific forms without altering the technical concept or essential features of the embodiments described above. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. For example, each component described as a single unit may be implemented in a distributed manner, and components described as distributed may likewise be implemented in a combined form.

[0080] The scope of protection sought through this specification is defined by the claims set forth below rather than by the detailed description above, and should be interpreted to include all modifications or variations derived from the meaning and scope of the claims and the concept of equivalents. Explanation of the symbols

[0082] 10: Usage reduction device through enhanced antibody response 100: Reaction chamber 200: Reactor 300: Spreader 400: Bulkhead 500: Protrusion 700: Ice pack mounting groove 800: Cover

Claims

Claim 1 A reaction vessel having an open top and a reaction chamber formed on the inside in the shape of a rounded arch facing downward, so that an antibody solution can be received and a blotting membrane that reacts with the antibody solution can be disposed therein; and a cylindrical spreader disposed above the blotting membrane within the reaction chamber and moving by rolling back and forth along the bottom surface of the reaction chamber as the reaction vessel tilts in the front-rear direction; wherein the reaction chamber comprises: a chamber space having an open top and a rounded arch shape on the bottom; a receiving pouch made of a thin film capable of expansion and contraction, installed along the lower corners of one side and the other side of the chamber space to cover and cover the bottom of the chamber space; a first flat magnetic body made of a soft, square-shaped thin film magnetic body attached and installed along the bottom surface of the receiving pouch; a first support plate made of a square flat plate shape formed by being rounded in correspondence with the curvature of the bottom surface of the chamber space, with its front end connected and installed above the front end of the chamber space and seated along a part of the front end of the bottom surface of the chamber space to support the first flat magnetic body; and a square flat plate shape formed by being rounded in correspondence with the curvature of the bottom surface of the chamber space, with its rear end being the chamber A second support plate connected and installed on the upper rear end of the space and seated along the remaining portion of the bottom surface of the chamber space to support the first flat magnetic body; at least one extension formed extending from the rear end of the first support plate opposite to the second support plate; at least one seating groove formed recessed from the front end of the second support plate opposite to the first support plate so that the extension can be positioned; and a second flat magnetic body made of a soft thin film magnetic body having opposite polarity to the first support plate, attached and installed along the upper side of the first support plate and the extension to press the receiving pouch against the first support plate.A device for reducing usage through improved antibody response, comprising: a third flat magnetic body made of a soft thin film magnetic body with opposite polarity to the first support plate, attached along the upper side of the second support plate to bring the receiving pouch into close contact with the second support plate; a gear tooth formed along the lower side of the extension; and a driving gear connected to the lower front side of the second support plate to enable rotational driving, which engages with the gear tooth through gear coupling, and rotates in a forward or reverse direction to insert or expose the extension into the seating groove, thereby bringing the first support plate and the second support plate into close contact or apart, and adjusting the arrangement angle of the first support plate and the second support plate. Claim 2 In claim 1, the spreader rotates and moves back and forth along the bottom surface of the reaction chamber to evenly spread the antibody solution applied to it onto the blotting membrane, thereby reducing usage through antibody reaction enhancement. Claim 3 In claim 1, the reaction vessel comprises at least one partition wall for partitioning the internal space of the reaction chamber, a device for reducing usage through antibody reaction enhancement. Claim 4 In claim 1, the reaction vessel has at least one protrusion formed at the front and rear ends of the bottom surface of the reaction chamber to limit the movement range of the spreader and the blotting membrane, the reaction vessel prevents the spreader from becoming crooked during movement by aligning it when tilted and facilitates the movement of the reaction chamber, and the reaction vessel has an ice pack mounting groove formed on the lower side to accommodate an ice pack for a cooling effect, a device for reducing usage through improved antibody reaction. Claim 5 A device for reducing usage through improved antibody reaction, further comprising: a cover seated on the upper side of the reaction vessel to open and close the upper side of the reaction chamber to reduce the evaporation of the antibody solution in the first aspect. Claim 6 delete

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