A method for obtaining 2-(4-(ethoxycarbonyl)phenyl)acetic acid from the endophytic fungus neurospora dictyophora

Abstract

The method for obtaining 2-(4(ethoxycarbonyl)phenyl)acetic acid from the endophytic fungus Neurospora dictyophora, isolated from the lichen Evernia sp., which has antimicrobial activity, relates to the field of bioorganic chemistry, microbiology, biotechnology and pharmacology, in particular, to the production of aromatic acids from a natural source that have promising biological activity. The technical objective of the utility model is to develop an effective method for isolating 2-(4-(ethoxycarbonyl)phenyl)acetic acid (C11H12O4, molecular weight 208 g / mol) from the endophytic fungus Neurospora dictyophora isolated from the lichen Evernia sp., in the Republic of Kazakhstan. The technical result is achieved by isolating 2-(4-(ethoxycarbonyl)phenyl)acetic acid from the endophytic fungus Neurospora dictyophora isolated from the lichen Evernia sp. (family Parmeliaceae). The isolated compound demonstrates potential antimicrobial activity, the diameter of the zones of inhibition of microorganism growth was 21-22 mm. The method includes: identification of the lichen by thin-layer chromatography, isolation of the fungus from the lichen Evernia sp., identification of the fungus by analysis of the nucleotide sequence of the ITS gene, cultivation of the fungus Neurospora dictyophora, chromatographic separation of the extract, isolation of 2-(4(ethoxycarbonyl)phenyl)acetic acid and its identification by NMR spectroscopy, determination of antimicrobial activity. The utility model's effectiveness lies in the isolation of a biologically active compound from natural sources (the lichen Evernia sp.). 2-(4(ethoxycarbonyl)phenyl)acetic acid is a promising substance as an antimicrobial agent. Therefore, the application of our proposed methodology opens up prospects for its use in the pharmaceutical, biotechnology and chemical industries, which will contribute to the development of sustainable production and the implementation of the principles of “green” chemistry.