3-(1-OXOISOINDOLIN-2-YL)PIPERIDINE-2,6-DIONE DERIVATIVES AND THEIR USES

Description

RELATED APPLICATIONS

[0001] This application claims the benefit of and priority to U.S. Provisional application No. 62 / 549,225, filed August 23, 2017.FIELD OF THE INVENTION

[0002] The present invention relates to 3-(1-oxoisoindolin-2-yl)piperidine-2,6-dione compounds, compositions thereof and said compounds and compositions for use in the treatment of IKAROS Family Zinc Finger 2 (IKZF2)-dependent diseases or disorders or where reduction of IKZF2 or IKZF4 protein levels can ameliorate a disease or disorder.BACKGROUND OF THE INVENTION

[0003] IKAROS Family Zinc Finger 2 (IKZF2) (also known as Helios) is one of the five members of the Ikaros family of transcription factors found in mammals. IKZF2 contains four zinc finger domains near the N-terminus which are involved in DNA binding and two zinc finger domains at the C-terminus which are involved in protein dimerization. IKZF2 is about 50% identical with Ikaros family members, Ikaros (IKZF1), Aiolos (IKZF3), and Eos (IKZF4) with highest homology in the zinc finger regions (80%+ identity). These four Ikaros family transcription factors bind to the same DNA consensus site and can heterodimerize with each other when co-expressed in cells. The fifth Ikaros family protein, Pegasus (IKZF5), is only 25% identical to IKZF2, binds a different DNA site than other Ikaros family members and does not readily heterodimerize with the other Ikaros family proteins. IKZF2, IKZF1 and IKZF3 are expressed mainly in hematopoietic cells while IKZF4 and IKZF5 are expressed in a wide variety of tissues. (John, L.B., et al., (2011), Mol. Immunol. 48:1272-1278; Perdomo, J., et al., (2000), J. Biol. Chem. 275:38347-38354.)

[0004] IKZF2 is believed to have an important role in the function and stability of regulatory T cells (Tregs). IKZF2 is highly expressed at the mRNA and protein level by regulatory T-cell populations. Knockdown of IKZF2 by siRNA has been shown to result in downregulation of FoxP3 and to impair the ability of isolated human CD4+ CD25+ Tregs to block T-cell activation in vitro. Moreover, overexpression of IKZF2 in isolated murine Tregs has been shown to increase expression of Treg related markers such as CD103 and GITR and the IKZF2 overexpressing cells showed increased suppression of responder T-cells. IKZF2 has also been found to bind the promoter of FoxP3, the defining transcription factor of the regulatory T-cell lineage, and to affect FoxP3 expression.

[0005] Knockout of IKZF2 within FoxP3-expressing Tregs in mice has been shown to cause activated Tregs to lose their inhibitory properties, to express T-effector cytokines, and to take on T-effector functions. IKZF2 knockout mutant mice develop autoimmune disease by 6-8 months of age, with increased numbers of activated CD4 and CD8 T cells, follicular helper T cells and germinal center B cells. This observed effect is believed to be cell intrinsic, as Rag2- / - mice given bone marrow from IKZF2 knockout mice, but not bone marrow from IKZF2+ / + develop autoimmune disease. Direct evidence that IKZF2 affects regulatory T-cell function has been shown in the analysis of mice in which IKZF2 was deleted only in FoxP3 expressing cells (FoxP3-YFP-Cre Heliosfl / fl). The results showed that the mice also develop autoimmune disease with similar features as observed in the whole animal IKZF2 knockout. Moreover, pathway analysis of a CHIP-SEQ experiment has also suggested that IKZF2 is affecting expression of genes in the STAT5 / IL-2Rα pathway in regulatory T-cells. This effect of IKZF2 loss was shown to be more apparent after an immune challenge (viral infection or injection with sheep's blood), and it was noted that after immune stimulation, the IKZF2 negative regulatory T cells began to take on features of effector T cells. (Getnet, D., et al., Mol. Immunol. (2010), 47:1595-1600; Bin Dhuban, K.., et al., (2015), J. Immunol. 194 :3687-96; Kim, H-J., et al., (2015), Science 350 :334-339; Nakawaga, H., et al., (2016) PNAS, 113: 6248-6253)

[0006] Overexpression of Ikaros isoforms which lack the DNA binding regions have been shown to be associated with multiple human haematological malignancies. Recently, mutations in the IKZF2 gene, which lead to abnormal splicing variants, have been identified in adult T-cell leukemias and low hypodiploid acute lymphoblastic leukemia. It has been proposed that these isoforms, which are capable of dimerization, have a dominant negative effect on Ikaros family transcription factors which primes the development of lymphomas. IKZF2 knockout mutants that survive into adulthood do not develop lymphomas, supporting this hypothesis (Asanuma, S., et al., (2013), Cancer Sci. 104:1097-1106; Zhang, Z., et al., (2007), Blood 109:2190-2197; Kataoka, D., et al., (2015), Nature Genetics 47:1304-1315.)

[0007] Currently, anti-CTLA4 antibodies are used in the clinic to target Tregs in tumors. However, targeting CTLA4 often causes systemic activation of T-effector cells, resulting in excessive toxicity and limiting therapeutic utility. Up to 3 / 4 of patients treated with a combination of anti-PD1 and anti-CTLA4 have reported grade 3 or higher adverse events. Thus, a strong need exists to provide compounds that target Tregs in tumors without causing systemic activation of T-effector cells.

[0008] An IKZF2-specific degrader has the potential to focus the enhanced immune response to areas within or near tumors providing a potentially more tolerable and less toxic therapeutic agent for the treatment of cancer.

[0009] WO2015 / 160845 discloses piperidinyl-2,6-dione compounds for use in the treatment of cancer.

[0010] WO2006 / 028964 discloses substituted 2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindoline compounds and processes of making.

[0011] EP3061758 discloses 5-substituted isoindoline compounds for use in the treatment of various diseases or disorders.SUMMARY OF THE INVENTION

[0012] The compounds of the invention have use as therapeutic agents, particularly for cancers and related diseases. In one aspect, the compounds of the invention have IKZF2 degrader activity, preferably having such activity at or below the 50 µM level, and more preferably having such activity at or below the 10 µM level. In another aspect, the compounds of the invention have degrader activity for IKZF2 that is selective over one or more of IKZF1, IKZF3, IKZF4, and / or IKZF5. In another aspect, the compounds of the invention have degrader activity for both IKZF2 and IKZF4. The compounds of the invention have usefulness in treating cancer and other diseases for which such degrader activity would be beneficial for the patient. For example, while not intending to be bound by any theory, the inventors believe that reducing levels of IKZF2 in Tregs in a tumor may allow the patient immune system to more effectively attack the disease. In summary, the present invention provides novel IKZF2 degraders useful for the treatment of cancer and other diseases.

[0013] A first aspect of the present invention relates to compounds of Formula (I') wherein: X 1 is CR 3 ; is optionally a double bond when X 1 is CR 3 and R 3 is absent; each R 1 is independently (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )hydroxyalkyl, or halogen, or two R 1 together with the carbon atoms to which they are attached form a 5- or 6- membered heterocycloalkyl ring, or two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S; R 2 is H, (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, -C(O)O(CH 2 ) 0-3 (C 6 -C 10 )aryl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one or more R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one or more R 5 , or R 1 and R 2 , when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heterocycloalkyl ring; R 3 is H or R 3 is absent when is a double bond; each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , -NR 6 C(O)R 6' , halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 4 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one or more R 7 ; each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one or more R 10 ; R 6 and R 6' are each independently H, (C 1 -C 6 )alkyl, or (C 6 -C 10 )aryl; each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -(CH 2 ) 0-3 C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , - NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, adamantyl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one or more R 11 , and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one or more substituents each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy,or two R 7 together with the carbon atom to which they are attached form a =(O), or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 , or two R 7 together with the atoms to which they are attached form a (C 5 -C 7 ) cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 ; F 8 and R 9 are each independently H or (C 1 -C 6 )alkyl; each R 10 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy,(C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN, or two R 10 together with the carbon atom to which they are attached form a =(O); each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy,(C 6 -C 10 )aryl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one or more substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy,(C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN; R 12 is (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, (C 6 -C 10 )aryl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S; R x isH orD; p is 0, 1, or 2; n is 0, 1, or 2; n1 is 1 or 2, wherein n + n1 ≤ 3; and q is 0, 1, 2, 3, or 4; or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0014] In one embodiment, the present invention relates to compounds of Formula (I')having the structure of Formula (I): wherein: X 1 is CR 3 ; is optionally a double bond when X 1 is CR 3 and R 3 is absent; each R 1 is independently (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )hydroxyalkyl, or halogen; R 2 is H, (C 1 -C 6 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one or more R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one or more R 5 ; R 3 is H or R 3 is absent when is a double bond; each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , -NR 6 C(O)R 6' , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one or more R 7 ; each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one or more R 10 ; R 6 and R 6' are each independently H or (C 1 -C 6 )alkyl; each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy,(C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 , or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 ; R 8 and R 9 are each independently H or (C 1 -C 6 )alkyl; each R 10 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN; R x is H or D; n is 1 or 2; and q is 0, 1, 2, 3, or 4, or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0015] In one aspect of the invention, the hydrogens in the compound of Formula (I') or Formula (I) are present in their normal isotopic abundances. In a preferred aspect of the invention, the hydrogens are isotopically enriched in deuterium (D), and in a particularly preferred aspect of the invention the hydrogen at position R x is enriched in D, as discussed in more detail concerning isotopes and isotopic enrichment below.

[0016] Another aspect of the present invention relates to a pharmaceutical composition comprising a therapeutically effective amount of a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, and a pharmaceutically acceptable carrier or excipient. The pharmaceutical composition is useful in the treatment of IKZF2-dependent diseases or disorders. The pharmaceutical composition may further comprise at least one additional pharmaceutical agent.

[0017] Another aspect of the present invention relates to a pharmaceutical composition comprising a therapeutically effective amount of a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, and a pharmaceutically acceptable carrier or excipient for use in the treatment of an IKZF2-dependent disease or disorder.

[0018] Another aspect of the present invention relates to a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of an IKZF2-dependent disease or disorder.

[0019] In another aspect, the present invention relates to a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of cancer, wherein the cancer is selected from non-small cell lung cancer (NSCLC), melanoma, triple-negative breast cancer (TNBC), nasopharyngeal cancer (NPC), microsatellite stable colorectal cancer (mssCRC), thymoma, carcinoid, and gastrointestinal stromal tumor (GIST). In another embodiment, the cancer is selected from non-small cell lung cancer (NSCLC), melanoma, triple-negative breast cancer (TNBC), nasopharyngeal cancer (NPC), and microsatellite stable colorectal cancer (mssCRC).

[0020] Another aspect of the present invention relates to a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of a cancer for which the immune response is deficient or an immunogenic cancer.

[0021] In another aspect of the invention, the compounds according to the disclosure are formulated into pharmaceutical compositions comprising an effective amount, preferably a pharmaceutically effective amount, of a compound according to the disclosure or salt, hydrate, solvate, stereoisomer, or tautomer thereof, and a pharmaceutically acceptable excipient or carrier.

[0022] The present invention provides degraders of IKZF2 that are therapeutic agents in the treatment of diseases such as cancer and metastasis, in the treatment of diseases affected by the modulation of IKZF2 protein levels, and in the treatment IKZF2-dependent diseases or disorders.

[0023] In one embodiment, the disease or disorder that can be treated by the compounds of the present disclosure is selected from non-small cell lung cancer (NSCLC), melanoma, triple-negative breast cancer (TNBC), nasopharyngeal cancer (NPC), microsatellite stable colorectal cancer (mssCRC), thymoma, carcinoid, gastrointestinal stromal tumor (GIST), prostate cancer, breast carcinoma, lymphomas, leukaemia, myeloma, bladder carcinoma, colon cancer, cutaneous melanoma, hepatocellular carcinoma, endometrial cancer, ovarian cancer, cervical cancer, lung cancer, renal cancer, glioblastoma multiform, glioma, thyroid cancer, parathyroid tumor, nasopharyngeal cancer, tongue cancer, pancreatic cancer, esophageal cancer, cholangiocarcinoma, gastric cancer, soft tissue sarcomas, rhabdomyosarcoma (RMS), synovial sarcoma, osteosarcoma, rhabdoid cancers, and Ewing's sarcoma. In another embodiment, the IKZF2-dependent disease or disorder is a cancer for which the immune response is deficient or an immunogenic cancer.

[0024] The present invention provides agents with novel mechanisms of action toward IKZF2 proteins in the treatment of various types of diseases including cancer and metastasis, in the treatment of diseases affected by the modulation of IKZF2 protein levels, and in the treatment IKZF2-dependent diseases or disorders. Ultimately a novel pharmacological strategy for the treatment of diseases and disorders associated with IKZF2 proteins is described for the attention of the medical community.

[0025] The present invention provides agents with novel mechanisms of action toward IKZF2 proteins in the treatment of various types of diseases including cancer and metastasis, in the treatment of diseases affected by the modulation of IKZF2 protein levels, and in the treatment IKZF2-dependent diseases or disorders. Ultimately a novel pharmacological strategy for the treatment of diseases and disorders associated with IKZF2 proteins is described for the attention of the medical community.

[0026] Thus, the present invention therefore provides compounds of formula (I') or (I) or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof, and also provides these compounds, pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof and pharmaceutical compositions thereof, for use in the treatment of cancer, especially in the treatment of cancers described in the claims.

[0027] Various embodiments of the invention are described herein and in the claims.BRIEF DESCRIPTION OF THE DRAWINGS

[0028] FIG. 1. is a bar graph showing the effects on cell expansion in purified primary human Treg cells when treated with DMSO (Control) or Compound 1-57. The results in FIG. 1 show that the expansion of purified Treg cells is impaired when treated with Compound 1-57 as compared to the control. FIG. 2. is a box-and-whiskers graph showing the effects on IL2 levels in purified primary human Treg cells when treated with DMSO (Control) or Compound 1-57. For each treatment, each dot represents one of five donors. The results in FIG. 2 show that production of IL2 is enhanced in Treg cells treated with Compound 1-57 as compared to the control. FIG. 3. is a box-and-whiskers graph showing the effects on in vitro suppression of the proliferation of CD4+ T cells in purified primary human Treg cells when expanded in the presence of DMSO (Control) or Compound 1-57. For each treatment, each dot represents one of five donors. The results in FIG. 3 show that in vitro suppression of the proliferation of CD4+ T Cells is impaired in human Treg cells when treated with Compound 1-57 as compared to the control. FIG. 4. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound 1-43 using the Prolabel assay. The results in FIG. 4 show that the levels of IKZF2 are decreased when treated with Compound 1-43 as compared to the control. FIG. 5. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound 1-57 using the Prolabel assay. The results in FIG. 5 show that the levels of IKZF2 are decreased when treated with Compound 1-57 as compared to the control. FIG. 6. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GTcells when treated with DMSO (Control) or Compound 1-68 using the Prolabel assay. The results in FIG. 6 show that the levels of IKZF2 are decreased when treated with Compound 1-68 as compared to the control. FIG. 7. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound 1-69 using the Prolabel assay. The results in FIG. 7 show that the levels of IKZF2 are decreased when treated with Compound 1-69 as compared to the control. FIG. 8. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound I-136 using the Prolabel assay. The results in FIG. 8 show that the levels of IKZF2 are decreased when treated with Compound I-136 as compared to the control. FIG. 9. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound 1-147 using the Prolabel assay. The results in FIG. 9 show that the levels of IKZF2 are decreased when treated with Compound 1-147 as compared to the control. FIG. 10. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound I-219 using the Prolabel assay. The results in FIG. 10 show that the levels of IKZF2 are decreased when treated with Compound I-219 as compared to the control. FIG. 11. is a bar graph showing the percentage change of IKZF2 protein levels in HEK293GT cells when treated with DMSO (Control) or Compound I-236 using the Prolabel assay. The results in FIG. 11 show that the levels of IKZF2 are decreased when treated with Compound I-236 as compared to the control. DETAILED DESCRIPTION OF THE INVENTION

[0029] The present invention relates to compounds and compositions that are capable of modulating IKZF2 protein levels. In one aspect, the compounds of the invention have use as therapeutic agents, particularly for cancers and related diseases. In one aspect, the compounds of the invention have IKZF2 degradation activity, preferably having such activity at or below the 50 µM level, and more preferably having such activity at or below the 10 µM level. In another aspect, the compounds of the invention have degrader activity for IKZF2 that is selective over one or more of IKZF1, IKZF3, IKZF4, and / or IKZF5. In another aspect, the compounds of the invention have degrader activity for both IKZF2 and IKZF4. The compounds of the invention have usefulness in treating cancer and other diseases for which such degradation activity would be beneficial for the patient. For example, while not intending to be bound by any theory, the inventors believe that reducing levels of IKZF2 in Tregs in a tumor may allow the patient immune system to more effectively attack the disease. In summary, the present invention provides novel IKZF2 degraders useful for the treatment of cancer and other diseases.

[0030] In a first aspect of the invention, the compounds of Formula (I') are described: or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof, wherein R 1 , R 2 , R x , X 1 , n, n1, and q are as described herein above.

[0031] The details of the invention are set forth in the accompanying description below. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, illustrative methods and materials are now described. Other features, objects, and advantages of the invention will be apparent from the description and from the claims. In the specification and the appended claims, the singular forms also include the plural unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.Definition of Terms and Conventions Used

[0032] Terms not specifically defined herein should be given the meanings that would be given to them by one of skill in the art in light of the disclosure and the context. As used in the specification and appended claims, however, unless specified to the contrary, the following terms have the meaning indicated and the following conventions are adhered to.A. Chemical Nomenclature, Terms, and Conventions

[0033] In the groups, radicals, or moieties defined below, the number of carbon atoms is often specified preceding the group, for example, (C 1 -C 10 )alkyl means an alkyl group or radical having 1 to 10 carbon atoms. In general, for groups comprising two or more subgroups, the last named group is the radical attachment point, for example, "alkylaryl" means a monovalent radical of the formula alkyl-aryl-, while "arylalkyl" means a monovalent radical of the formula aryl-alkyl-. Furthermore, the use of a term designating a monovalent radical where a divalent radical is appropriate shall be construed to designate the respective divalent radical and vice versa. Unless otherwise specified, conventional definitions of terms control and conventional stable atom valences are presumed and achieved in all formulas and groups. The articles "a" and "an" refer to one or more than one (e.g., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element.

[0034] The term "and / or" means either "and" or "or" unless indicated otherwise.

[0035] The term "optionally substituted" means that a given chemical moiety (e.g., an alkyl group) can (but is not required to) be bonded other substituents (e.g., heteroatoms). For instance, an alkyl group that is optionally substituted can be a fully saturated alkyl chain (e.g., a pure hydrocarbon). Alternatively, the same optionally substituted alkyl group can have substituents different from hydrogen. For instance, it can, at any point along the chain be bounded to a halogen atom, a hydroxyl group, or any other substituent described herein. Thus, the term "optionally substituted" means that a given chemical moiety has the potential to contain other functional groups, but does not necessarily have any further functional groups. Suitable substituents used in the optional substitution of the described groups include, without limitation, halogen, oxo, -OH, -CN, -COOH, -CH 2 CN, -O-(C 1 -C 6 )alkyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -O-(C 2 -C 6 )alkenyl, -O-(C 2 -C 6 )alkynyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, -OH, - OP(O)(OH) 2 , -OC(O)(C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -OC(O)O(C 1 -C 6 )alkyl, -NH 2 , -NH((C 1 -C 6 )alkyl), - N((C 1 -C 6 )alkyl) 2 , -NHC(O)(C 1 -C 6 )alkyl, -C(O)NH(C 1 -C 6 )alkyl, -S(O) 2 (C 1 -C 6 )alkyl, -S(O)NH(C 1 -C 6 )alkyl, and S(O)N((C 1 -C 6 )alkyl) 2 . The substituents can themselves be optionally substituted. "Optionally substituted" as used herein also refers to substituted or unsubstituted whose meaning is described below.

[0036] The term "substituted" means that the specified group or moiety bears one or more suitable substituents wherein the substituents may connect to the specified group or moiety at one or more positions. For example, an aryl substituted with a cycloalkyl may indicate that the cycloalkyl connects to one atom of the aryl with a bond or by fusing with the aryl and sharing two or more common atoms.

[0037] The term "unsubstituted" means that the specified group bears no substituents.

[0038] Unless otherwise specifically defined, "aryl" means a cyclic, aromatic hydrocarbon group having 1 to 3 aromatic rings, including monocyclic or bicyclic groups such as phenyl, biphenyl, or naphthyl. When containing two aromatic rings (bicyclic, etc.), the aromatic rings of the aryl group are optionally joined at a single point (e.g., biphenyl), or fused (e.g., naphthyl). The aryl group is optionally substituted by one or more substituents, e.g., 1 to 5 substituents, at any point of attachment. Exemplary substituents include, but are not limited to, -H, -halogen, -CN, -O-(C 1 -C 6 )alkyl, (C 1 -C 6 )alkyl, -O-(C 2 -C 6 )alkenyl, -O-(C 2 -C 6 )alkynyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, -OH, -OP(O)(OH) 2 , -OC(O)(C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, - OC(O)O(C 1 -C 6 ) alkyl, NH 2 , NH((C 1 -C 6 )alkyl), N((C 1 -C 6 )alkyl) 2 , -S(O) 2 -(C 1 -C 6 )alkyl, -S(O)NH(C 1 -C 6 )alkyl, and S(O)N((C 1 -C 6 )alkyl) 2 . The substituents are themselves optionally substituted. Furthermore, when containing two fused rings, the aryl groups optionally have an unsaturated or partially saturated ring fused with a fully saturated ring. Exemplary ring systems of these aryl groups include, but are not limited to, phenyl, biphenyl, naphthyl, anthracenyl, phenalenyl, phenanthrenyl, indanyl, indenyl, tetrahydronaphthalenyl, tetrahydrobenzoannulenyl, and the like.

[0039] Unless otherwise specifically defined, "heteroaryl" means a monovalent monocyclic aromatic radical of 5 to 24 ring atoms or a polycyclic aromatic radical, containing one or more ring heteroatoms selected from N, O, or S, the remaining ring atoms being C. Heteroaryl as herein defined also means a bicyclic heteroaromatic group wherein the heteroatom is selected from N, O, or S. The aromatic radical is optionally substituted independently with one or more substituents described herein. Examples include, but are not limited to, furyl, thienyl, pyrrolyl, pyridyl, pyrazolyl, pyrimidinyl, imidazolyl, isoxazolyl, oxazolyl, oxadiazolyl, pyrazinyl, indolyl, thiophen-2-yl, quinolyl, benzopyranyl, isothiazolyl, thiazolyl, thiadiazole, indazole, benzimidazolyl, thieno[3,2-b]thiophene, triazolyl, triazinyl, imidazo[1,2-b]pyrazolyl, furo[2,3-c]pyridinyl, imidazo[1,2-a]pyridinyl, indazolyl, pyrrolo[2,3-c]pyridinyl, pyrrolo[3,2-c]pyridinyl, pyrazolo[3,4-c]pyridinyl, thieno[3,2-c]pyridinyl, thieno[2,3-c]pyridinyl, thieno[2,3-b]pyridinyl, benzothiazolyl, indolyl, indolinyl, indolinonyl, dihydrobenzothiophenyl, dihydrobenzofuranyl, benzofuran, chromanyl, thiochromanyl, tetrahydroquinolinyl, dihydrobenzothiazine, dihydrobenzoxanyl, quinolinyl, isoquinolinyl, 1,6-naphthyridinyl, benzo[de]isoquinolinyl, pyrido[4,3-b][1,6]naphthyridinyl, thieno[2,3-b]pyrazinyl, quinazolinyl, tetrazolo[1,5-a]pyridinyl, [1,2,4]triazolo[4,3-a]pyridinyl, isoindolyl, pyrrolo[2,3-b]pyridinyl, pyrrolo[3,4-b]pyridinyl, pyrrolo[3,2-b]pyridinyl, imidazo[5,4-b]pyridinyl, pyrrolo[1,2-a]pyrimidinyl, tetrahydropyrrolo[1,2-a]pyrimidinyl, 3,4-dihydro-2H-1Δ 2< -pyrrolo[2,1-b]pyrimidine, dibenzo[b,d]thiophene, pyridin-2-one, furo[3,2-c]pyridinyl, furo[2,3-c]pyridinyl, 1H-pyrido[3,4-b][1,4]thiazinyl, benzooxazolyl, benzoisoxazolyl, furo[2,3-b]pyridinyl, benzothiophenyl, 1,5-naphthyridinyl, furo[3,2-b]pyridine, [1,2,4]triazolo[1,5-a]pyridinyl, benzo[1,2,3]triazolyl, imidazo[1,2-a]pyrimidinyl, [1,2,4]triazolo[4,3-b]pyridazinyl, benzo[c][1,2,5]thiadiazolyl, benzo[c][1,2,5]oxadiazole, 1,3-dihydro-2H-benzo[d]imidazol-2-one, 3,4-dihydro-2H-pyrazolo[1,5-b][1,2]oxazinyl, 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridinyl, thiazolo[5,4 d]thiazolyl, imidazo[2,1-b][1,3,4]thiadiazolyl, thieno[2,3-b]pyrrolyl, 3H-indolyl, and derivatives thereof. Furthermore, when containing two fused rings the aryl groups herein defined may have an unsaturated or partially saturated ring fused with a fully saturated ring. Exemplary ring systems of these heteroaryl groups include indolinyl, indolinonyl, dihydrobenzothiophenyl, dihydrobenzofuran, chromanyl, thiochromanyl, tetrahydroquinolinyl, dihydrobenzothiazine,3,4-dihydro-1H-isoquinolinyl, 2,3-dihydrobenzofuran, indolinyl, indolyl, and dihydrobenzoxanyl.

[0040] Halogen or "halo" mean fluorine, chlorine, bromine, or iodine.

[0041] "Alkyl" means a straight or branched chain saturated hydrocarbon containing 1-12 carbon atoms. Examples of a (C 1 -C 6 )alkyl group include, but are not limited to, methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl, sec-butyl, tert-butyl, isopentyl, neopentyl, and isohexyl.

[0042] "Alkoxy" means a straight or branched chain saturated hydrocarbon containing 1-12 carbon atoms containing a terminal "O" in the chain, e.g., -O(alkyl). Examples of alkoxy groups include, without limitation, methoxy, ethoxy, propoxy, butoxy, t-butoxy, or pentoxy groups.

[0043] "Alkenyl" means a straight or branched chain unsaturated hydrocarbon containing 2-12 carbon atoms. The "alkenyl" group contains at least one double bond in the chain. The double bond of an alkenyl group can be unconjugated or conjugated to another unsaturated group. Examples of alkenyl groups include ethenyl, propenyl, n-butenyl, iso-butenyl, pentenyl, or hexenyl. An alkenyl group can be unsubstituted or substituted and may be straight or branched.

[0044] "Alkynyl" means a straight or branched chain unsaturated hydrocarbon containing 2-12 carbon atoms. The "alkynyl" group contains at least one triple bond in the chain. Examples of alkenyl groups include ethynyl, propargyl, n-butynyl, iso-butynyl, pentynyl, or hexynyl. An alkynyl group can be unsubstituted or substituted.

[0045] "Alkylene" or "alkylenyl" means a divalent alkyl radical. Any of the above mentioned monovalent alkyl groups may be an alkylene by abstraction of a second hydrogen atom from the alkyl. As herein defined, alkylene may also be a (C 1 -C 6 )alkylene. An alkylene may further be a (C 1- C 4 )alkylene. Typical alkylene groups include, but are not limited to, -CH 2 -, -CH(CH 3 )-, -C(CH 3 ) 2 -, -CH 2 CH 2 -, -CH 2 CH(CH 3 )-, - CH 2 C(CH 3 ) 2 -, -CH 2 CH 2 CH 2 -, -CH 2 CH 2 CH 2 CH-, and the like.

[0046] "Cycloalkyl" or "carbocyclyl" means a monocyclic or polycyclic saturated carbon ring containing 3-18 carbon atoms. Examples of cycloalkyl groups include, without limitations, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptanyl, cyclooctanyl, norboranyl, norborenyl, bicyclo[2.2.2]octanyl, or bicyclo[2.2.2]octenyl and derivatives thereof. A (C 3 -C 8 )cycloalkyl is a cycloalkyl group containing between 3 and 8 carbon atoms. A cycloalkyl group can be fused (e.g., decalin) or bridged (e.g., norbornane).

[0047] "Heterocyclyl" or "heterocycloalkyl" means a saturated or partially saturated monocyclic or polycyclic ring containing carbon and at least one heteroatom selected from oxygen, nitrogen, or sulfur (O, N, or S) and wherein there is not delocalized n electrons (aromaticity) shared among the ring carbon or heteroatoms. The heterocycloalkyl ring structure may be substituted by one or more substituents. The substituents can themselves be optionally substituted. Examples of heterocyclyl rings include, but are not limited to, oxetanyl, azetadinyl, tetrahydrofuranyl, tetrahydropyranyl, pyrrolidinyl, oxazolinyl, oxazolidinyl, thiazolinyl, thiazolidinyl, pyranyl, thiopyranyl, tetrahydropyranyl, dioxalinyl, piperidinyl, morpholinyl, thiomorpholinyl, thiomorpholinyl S-oxide, thiomorpholinyl S-dioxide, piperazinyl, azepinyl, oxepinyl, diazepinyl, tropanyl, oxazolidinonyl, 1,4-dioxanyl, dihydrofuranyl, 1,3-dioxolanyl, imidazolidinyl, imidazolinyl, dithiolanyl, and homotropanyl.

[0048] "Hydroxyalkyl" means an alkyl group substituted with one or more -OH groups. Examples of hydroxyalkyl groups include HO-CH 2 -, HO-CH 2 CH 2 -, and CH 2 -CH(OH)-.

[0049] "Haloalkyl" means an alkyl group substituted with one or more halogens. Examples of haloalkyl groups include, but are not limited to, trifluoromethyl, difluoromethyl, pentafluoroethyl, trichloromethyl, etc.

[0050] "Haloalkoxy" means an alkoxy group substituted with one or more halogens. Examples of haloalkyl groups include, but are not limited to, trifluoromethoxy, difluoromethoxy, pentafluoroethoxy, trichloromethoxy, etc.

[0051] "Cyano" means a substituent having a carbon atom joined to a nitrogen atom by a triple bond, e.g., C≡N.

[0052] "Amino" means a substituent containing at least one nitrogen atom (e.g., NH 2 ).

[0053] "Alkylamino" means an amino or NH 2 group where one of the hydrogens is replaced with an alkyl group, e.g., -NH(alkyl). Examples of alkylamino groups include, but are not limited to, methylamino (e.g., -NH(CH 3 )), ethylamino, propylamino, iso-propylamino, n-butylamino, sec-butylamino, tert-butylamino, etc.

[0054] "Dialkylamino" means an amino or NH 2 group where both of the hydrogens are replaced with alkyl groups, e.g., -N(alkyl) 2 . The alkyl groups on the amino group are the same or different alkyl groups. Examples of dialkylamino groups include, but are not limited to, dimethylamino (e.g., -N(CH 3 ) 2 ), diethylamino, dipropylamino, diiso-propylamino, di-n-butylamino, di-sec-butylamino, di-tert-butylamino, methyl(ethyl)amino, methyl(butylamino), etc.

[0055] "Spirocycloalkyl" or "spirocyclyl" means carbogenic bicyclic ring systems with both rings connected through a single atom. The rings can be different in size and nature, or identical in size and nature. Examples include spiropentane, spriohexane, spiroheptane, spirooctane, spirononane, or spirodecane. One or both of the rings in a spirocycle can be fused to another ring carbocyclic, heterocyclic, aromatic, or heteroaromatic ring. A (C 3 -C 12 )spirocycloalkyl is a spirocycle containing between 3 and 12 carbon atoms.

[0056] "Spiroheterocycloalkyl" or "spiroheterocyclyl" means a spirocycle wherein at least one of the rings is a heterocycle one or more of the carbon atoms can be substituted with a heteroatom (e.g., one or more of the carbon atoms can be substituted with a heteroatom in at least one of the rings). One or both of the rings in a spiroheterocycle can be fused to another ring carbocyclic, heterocyclic, aromatic, or heteroaromatic ring.

[0057] "Pomalidomide" or 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione has the following structure: B. Salt, Prodrug, Derivative, and Solvate Terms and Conventions

[0058] "Prodrug" or "prodrug derivative" mean a covalently-bonded derivative or carrier of the parent compound or active drug substance which undergoes at least some biotransformation prior to exhibiting its pharmacological effect(s). In general, such prodrugs have metabolically cleavable groups and are rapidly transformed in vivo to yield the parent compound, for example, by hydrolysis in blood, and generally include esters and amide analogs of the parent compounds. The prodrug is formulated with the objectives of improved chemical stability, improved patient acceptance and compliance, improved bioavailability, prolonged duration of action, improved organ selectivity, improved formulation (e.g., increased hydrosolubility), and / or decreased side effects (e.g., toxicity). In general, prodrugs themselves have weak or no biological activity and are stable under ordinary conditions. Prodrugs can be readily prepared from the parent compounds using methods known in the art, such as those described in A Textbook of Drug Design and Development, Krogsgaard-Larsen and H. Bundgaard (eds.), Gordon & Breach, 1991, particularly Chapter 5: "Design and Applications of Prodrugs"; Design of Prodrugs, H. Bundgaard (ed.), Elsevier, 1985; Prodrugs: Topical and Ocular Drug Delivery, K.B. Sloan (ed.), Marcel Dekker, 1998; Methods in Enzymology, K. Widder et al. (eds.), Vol. 42, Academic Press, 1985, particularly pp. 309-396; Burger's Medicinal Chemistry and Drug Discovery, 5th Ed., M. Wolff (ed.), John Wiley & Sons, 1995, particularly Vol. 1 and pp. 172-178 and pp. 949-982; Pro-Drugs as Novel Delivery Systems, T. Higuchi and V. Stella (eds.), Am. Chem. Soc., 1975; Bioreversible Carriers in Drug Design, E.B. Roche (ed.), Elsevier, 1987.

[0059] "Pharmaceutically acceptable prodrug" as used herein means a prodrug of a compound of the invention which is, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit / risk ratio, and effective for their intended use, as well as the zwitterionic forms, where possible.

[0060] "Salt" means an ionic form of the parent compound or the product of the reaction between the parent compound with a suitable acid or base to make the acid salt or base salt of the parent compound. Salts of the compounds of the present invention can be synthesized from the parent compounds which contain a basic or acidic moiety by conventional chemical methods. Generally, the salts are prepared by reacting the free base or acid parent compound with stoichiometric amounts or with an excess of the desired salt-forming inorganic or organic acid or base in a suitable solvent or various combinations of solvents.

[0061] "Pharmaceutically acceptable salt" means a salt of a compound of the invention which is, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit / risk ratio, generally water or oil-soluble or dispersible, and effective for their intended use. The term includes pharmaceutically-acceptable acid addition salts and pharmaceutically-acceptable base addition salts. As the compounds of the present invention are useful in both free base and salt form, in practice, the use of the salt form amounts to use of the base form. Lists of suitable salts are found in, e.g., S.M. Birge et al., J. Pharm. Sci., 1977, 66, pp. 1-19.

[0062] "Pharmaceutically-acceptable acid addition salt" means those salts which retain the biological effectiveness and properties of the free bases and which are not biologically or otherwise undesirable, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, sulfamic acid, nitric acid, phosphoric acid, and the like, and organic acids such as acetic acid, trichloroacetic acid, trifluoroacetic acid, adipic acid, alginic acid, ascorbic acid, aspartic acid, benzenesulfonic acid, benzoic acid, 2-acetoxybenzoic acid, butyric acid, camphoric acid, camphorsulfonic acid, cinnamic acid, citric acid, digluconic acid, ethanesulfonic acid, glutamic acid, glycolic acid, glycerophosphoric acid, hemisulfic acid, heptanoic acid, hexanoic acid, formic acid, fumaric acid, 2-hydroxyethanesulfonic acid (isethionic acid), lactic acid, maleic acid, hydroxymaleic acid, malic acid, malonic acid, mandelic acid, mesitylenesulfonic acid, methanesulfonic acid, naphthalenesulfonic acid, nicotinic acid, 2-naphthalenesulfonic acid, oxalic acid, pamoic acid, pectinic acid, phenylacetic acid, 3-phenylpropionic acid, picric acid, pivalic acid, propionic acid, pyruvic acid, pyruvic acid, salicylic acid, stearic acid, succinic acid, sulfanilic acid, tartaric acid, p-toluenesulfonic acid, undecanoic acid, and the like.

[0063] "Pharmaceutically-acceptable base addition salt" means those salts which retain the biological effectiveness and properties of the free acids and which are not biologically or otherwise undesirable, formed with inorganic bases such as ammonia or hydroxide, carbonate, or bicarbonate of ammonium or a metal cation such as sodium, potassium, lithium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like. Particularly preferred are the ammonium, potassium, sodium, calcium, and magnesium salts. Salts derived from pharmaceutically-acceptable organic nontoxic bases include salts of primary, secondary, and tertiary amines, quaternary amine compounds, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion-exchange resins, such as methylamine, dimethylamine, trimethylamine, ethylamine, diethylamine, triethylamine, isopropylamine, tripropylamine, tributylamine, ethanolamine, diethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperazine, piperidine, N-ethylpiperidine, tetramethylammonium compounds, tetraethylammonium compounds, pyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylmorpholine, dicyclohexylamine, dibenzylamine, N,N-dibenzylphenethylamine, 1-ephenamine, N,N'-dibenzylethylenediamine, polyamine resins, and the like. Particularly preferred organic nontoxic bases are isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine.

[0064] "Solvate" means a complex of variable stoichiometry formed by a solute, for example, a compound of Formula (I') or Formula (I)) and solvent, for example, water, ethanol, or acetic acid. This physical association may involve varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate will be capable of isolation, for example, when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. In general, such solvents selected for the purpose of the invention do not interfere with the biological activity of the solute. Solvates encompasses both solution-phase and isolatable solvates. Representative solvates include hydrates, ethanolates, methanolates, and the like.

[0065] "Hydrate" means a solvate wherein the solvent molecule(s) is / are water.

[0066] The compounds of the present invention as discussed below include the free base or acid thereof, their salts and solvates, particularly the pharmaceutically acceptable forms thereof. Such forms, particularly the pharmaceutically acceptable forms, are intended to be embraced by the appended claims.C. Isomer Terms and Conventions

[0067] "Isomers" means compounds having the same number and kind of atoms, and hence the same molecular weight, but differing with respect to the arrangement or configuration of the atoms in space. The term includes stereoisomers and geometric isomers.

[0068] "Stereoisomer" or "optical isomer" mean a stable isomer that has at least one chiral atom or restricted rotation giving rise to perpendicular dissymmetric planes (e.g., certain biphenyls, allenes, and spiro compounds) and can rotate plane-polarized light. Because asymmetric centers and other chemical structure exist in the compounds of the invention which may give rise to stereoisomerism, the invention contemplates stereoisomers and mixtures thereof. The compounds of the invention and their salts include asymmetric carbon atoms and may therefore exist as single stereoisomers, racemates, and as mixtures of enantiomers and diastereomers. Typically, such compounds will be prepared as a racemic mixture. If desired, however, such compounds can be prepared or isolated as pure stereoisomers, i.e., as individual enantiomers or diastereomers, or as stereoisomer-enriched mixtures. As discussed in more detail below, individual stereoisomers of compounds are prepared by synthesis from optically active starting materials containing the desired chiral centers or by preparation of mixtures of enantiomeric products followed by separation or resolution, such as conversion to a mixture of diastereomers followed by separation or recrystallization, chromatographic techniques, use of chiral resolving agents, or direct separation of the enantiomers on chiral chromatographic columns. Starting compounds of particular stereochemistry are either commercially available or are made by the methods described below and resolved by techniques well-known in the art.

[0069] "Enantiomers" means a pair of stereoisomers that are non-superimposable mirror images of each other.

[0070] "Diastereoisomers" or "diastereomers" mean optical isomers which are not mirror images of each other.

[0071] "Racemic mixture" or "racemate" mean a mixture containing equal parts of individual enantiomers.

[0072] "Non-racemic mixture" means a mixture containing unequal parts of individual enantiomers.

[0073] "Geometrical isomer" means a stable isomer which results from restricted freedom of rotation about double bonds (e.g., cis-2-butene and trans-2-butene) or in a cyclic structure (e.g., cis-1,3-dichlorocyclobutane and trans-1,3-dichlorocyclobutane). Because carbon-carbon double (olefinic) bonds, C=N double bonds, cyclic structures, and the like may be present in the compounds of the invention, the invention contemplates each of the various stable geometric isomers and mixtures thereof resulting from the arrangement of substituents around these double bonds and in these cyclic structures. The substituents and the isomers are designated using the cis / trans convention or using the E or Z system, wherein the term "E" means higher order substituents on opposite sides of the double bond, and the term "Z" means higher order substituents on the same side of the double bond. A thorough discussion of E and Z isomerism is provided in J. March, Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, 4th ed., John Wiley & Sons, 1992. Several of the following examples represent single E isomers, single Z isomers, and mixtures of E / Z isomers. Determination of the E and Z isomers can be done by analytical methods such as x-ray crystallography, 1< H NMR, and 13< C NMR.

[0074] Some of the compounds of the invention can exist in more than one tautomeric form. As mentioned above, the compounds of the invention include all such tautomers.

[0075] It is well-known in the art that the biological and pharmacological activity of a compound is sensitive to the stereochemistry of the compound. Thus, for example, enantiomers often exhibit strikingly different biological activity including differences in pharmacokinetic properties, including metabolism, protein binding, and the like, and pharmacological properties, including the type of activity displayed, the degree of activity, toxicity, and the like. Thus, one skilled in the art will appreciate that one enantiomer may be more active or may exhibit beneficial effects when enriched relative to the other enantiomer or when separated from the other enantiomer. Additionally, one skilled in the art would know how to separate, enrich, or selectively prepare the enantiomers of the compounds of the invention from this disclosure and the knowledge of the prior art.

[0076] Thus, although the racemic form of drug may be used, it is often less effective than administering an equal amount of enantiomerically pure drug; indeed, in some cases, one enantiomer may be pharmacologically inactive and would merely serve as a simple diluent. For example, although ibuprofen had been previously administered as a racemate, it has been shown that only the S-isomer of ibuprofen is effective as an anti-inflammatory agent (in the case of ibuprofen, however, although the R-isomer is inactive, it is converted in vivo to the S-isomer, thus, the rapidity of action of the racemic form of the drug is less than that of the pure S-isomer). Furthermore, the pharmacological activities of enantiomers may have distinct biological activity. For example, S-penicillamine is a therapeutic agent for chronic arthritis, while R-penicillamine is toxic. Indeed, some purified enantiomers have advantages over the racemates, as it has been reported that purified individual isomers have faster transdermal penetration rates compared to the racemic mixture. See U.S. Pat. Nos. 5,114,946 and 4,818,541.

[0077] Thus, if one enantiomer is pharmacologically more active, less toxic, or has a preferred disposition in the body than the other enantiomer, it would be therapeutically more beneficial to administer that enantiomer preferentially. In this way, the patient undergoing treatment would be exposed to a lower total dose of the drug and to a lower dose of an enantiomer that is possibly toxic or an inhibitor of the other enantiomer.

[0078] Preparation of pure enantiomers or mixtures of desired enantiomeric excess (ee) or enantiomeric purity are accomplished by one or more of the many methods of (a) separation or resolution of enantiomers, or (b) enantioselective synthesis known to those of skill in the art, or a combination thereof. These resolution methods generally rely on chiral recognition and include, for example, chromatography using chiral stationary phases, enantioselective host-guest complexation, resolution or synthesis using chiral auxiliaries, enantioselective synthesis, enzymatic and nonenzymatic kinetic resolution, or spontaneous enantioselective crystallization. Such methods are disclosed generally in Chiral Separation Techniques: A Practical Approach (2nd Ed.), G. Subramanian (ed.), Wiley-VCH, 2000; T.E. Beesley and R.P.W. Scott, Chiral Chromatography, John Wiley & Sons, 1999; and Satinder Ahuja, Chiral Separations by Chromatography, Am. Chem. Soc., 2000. Furthermore, there are equally well-known methods for the quantitation of enantiomeric excess or purity, for example, GC, HPLC, CE, or NMR, and assignment of absolute configuration and conformation, for example, CD ORD, X-ray crystallography, or NMR.

[0079] In general, all tautomeric forms and isomeric forms and mixtures, whether individual geometric isomers or stereoisomers or racemic or non-racemic mixtures, of a chemical structure or compound is intended, unless the specific stereochemistry or isomeric form is specifically indicated in the compound name or structure.D. Pharmaceutical Administration and Treatment Terms and Conventions

[0080] A "patient" or "subject" is a mammal, e.g., a human, mouse, rat, guinea pig, dog, cat, horse, cow, pig, or nonhuman primate, such as a monkey, chimpanzee, baboon or, rhesus. In certain embodiments, the subject is a primate. In yet other embodiments, the subject is a human.

[0081] An "effective amount" or "therapeutically effective amount" when used in connection with a compound means an amount of a compound of the present invention that (i) treats or prevents the particular disease, condition, or disorder, (ii) attenuates, ameliorates, or eliminates one or more symptoms of the particular disease, condition, or disorder, or (iii) prevents or delays the onset of one or more symptoms of the particular disease, condition, or disorder described herein.

[0082] The terms "pharmaceutically effective amount" or "therapeutically effective amount" means an amount of a compound according to the invention which, when administered to a patient in need thereof, is sufficient to effect treatment for disease-states, conditions, or disorders for which the compounds have utility. Such an amount would be sufficient to elicit the biological or medical response of a tissue, system, or patient that is sought by a researcher or clinician. The amount of a compound of according to the invention which constitutes a therapeutically effective amount will vary depending on such factors as the compound and its biological activity, the composition used for administration, the time of administration, the route of administration, the rate of excretion of the compound, the duration of treatment, the type of disease-state or disorder being treated and its severity, drugs used in combination with or coincidentally with the compounds of the invention, and the age, body weight, general health, sex, and diet of the patient. Such a therapeutically effective amount can be determined routinely by one of ordinary skill in the art having regard to their own knowledge, the prior art, and this invention.

[0083] As used herein, the term "pharmaceutical composition" refers to a compound of the invention, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, together with at least one pharmaceutically acceptable carrier, in a form suitable for oral or parenteral administration.

[0084] "Carrier" encompasses carriers, excipients, and diluents and means a material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting a pharmaceutical agent from one organ, or portion of the body, to another organ, or portion of the body of a subject.

[0085] A subject is "in need of" a treatment if such subject would benefit biologically, medically, or in quality of life from such treatment (preferably, a human).

[0086] As used herein, the term "inhibit", "inhibition", or "inhibiting" refers to the reduction or suppression of a given condition, symptom, or disorder, or disease, or a significant decrease in the baseline activity of a biological activity or process.

[0087] As used herein, the term "treat", "treating", or "treatment" of any disease or disorder refers to alleviating or ameliorating the disease or disorder (i.e., slowing or arresting the development of the disease or at least one of the clinical symptoms thereof); or alleviating or ameliorating at least one physical parameter or biomarker associated with the disease or disorder, including those which may not be discernible to the patient.

[0088] As used herein, the term "prevent", "preventing", or "prevention" of any disease or disorder refers to the prophylactic treatment of the disease or disorder; or delaying the onset or progression of the disease or disorder.

[0089] "Pharmaceutically acceptable" means that the substance or composition must be compatible chemically and / or toxicologically, with the other ingredients comprising a formulation, and / or the mammal being treated therewith.

[0090] "Disorder" means, and is used interchangeably with, the terms disease, condition, or illness, unless otherwise indicated.

[0091] "Administer", "administering", or "administration" means to either directly administering a disclosed compound or pharmaceutically acceptable salt of the disclosed compound or a composition to a subject, or administering a prodrug derivative or analog of the compound or pharmaceutically acceptable salt of the compound or composition to the subject, which can form an equivalent amount of active compound within the subject's body.

[0092] "Prodrug" means a compound which is convertible in vivo by metabolic means (e.g., by hydrolysis) to a disclosed compound.

[0093] "Compounds of the present disclosure", "Compounds of Formula (I')", "compounds of the disclosure", and equivalent expressions (unless specifically identified otherwise) refer to compounds of Formulae (I'), (I), (Ia), (Ib), (Ic), and (Id) as herein described including the tautomers, salts particularly the pharmaceutically acceptable salts, and the solvates and hydrates thereof, where the context so permits thereof, as well as all stereoisomers (including diastereoisomers and enantiomers), rotamers, tautomers, and isotopically labelled compounds (including deuterium substitutions), as well as inherently formed moieties (e.g., polymorphs, solvates and / or hydrates). For purposes of this disclosure, solvates and hydrates are generally considered compositions. In general and preferably, the compounds of the invention and the formulas designating the compounds of the invention are understood to only include the stable compounds thereof and exclude unstable compounds, even if an unstable compound might be considered to be literally embraced by the compound formula. Similarly, reference to intermediates, whether or not they themselves are claimed, is meant to embrace their salts and solvates, where the context so permits. For the sake of clarity, particular instances when the context so permits are sometimes indicated in the text, but these instances are purely illustrative and it is not intended to exclude other instances when the context so permits.

[0094] "Stable compound" or "stable structure" means a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic or diagnostic agent. For example, a compound which would have a "dangling valency" or is a carbanion is not a compound contemplated by the invention.

[0095] In a specific embodiment, the term "about" or "approximately" means within 20%, preferably within 10%, and more preferably within 5% of a given value or range.

[0096] The yield of each of the reactions described herein is expressed as a percentage of the theoretical yield. "Cancer" means any cancer caused by the proliferation of malignant neoplastic cells, such as tumors, neoplasms, carcinomas, sarcomas, leukemias, lymphomas, and the like. For example, cancers include, but are not limited to, mesothelioma, leukemias, and lymphomas such as cutaneous T-cell lymphomas (CTCL), noncutaneous peripheral T-cell lymphomas, lymphomas associated with human T-cell lymphotrophic virus (HTLV) such as adult T-cell leukemia / lymphoma (ATLL), B-cell lymphoma, acute nonlymphocytic leukemias, chronic lymphocytic leukemia, chronic myelogenous leukemia, acute myelogenous leukemia, lymphomas, and multiple myeloma, non-Hodgkin lymphoma, acute lymphatic leukemia (ALL), chronic lymphatic leukemia (CLL), Hodgkin's lymphoma, Burkitt lymphoma, adult T-cell leukemia lymphoma, acute-myeloid leukemia (AML), chronic myeloid leukemia (CML), or hepatocellular carcinoma. Further examples include myelodisplastic syndrome, childhood solid tumors such as brain tumors, neuroblastoma, retinoblastoma, Wilms' tumor, bone tumors, and soft-tissue sarcomas, common solid tumors of adults such as head and neck cancers (e.g., oral, laryngeal, and nasopharyngeal), esophageal cancer, genitourinary cancers (e.g., prostate, bladder, renal, uterine, ovarian, testicular), lung cancer (e.g., small-cell and non-small cell), breast cancer, pancreatic cancer, melanoma, and other skin cancers, stomach cancer, brain tumors, tumors related to Gorlin's syndrome (e.g., medulloblastoma, meningioma, etc.), and liver cancer. Additional exemplary forms of cancer which may be treated by the subject compounds include, but are not limited to, cancer of skeletal or smooth muscle, stomach cancer, cancer of the small intestine, rectum carcinoma, cancer of the salivary gland, endometrial cancer, adrenal cancer, anal cancer, rectal cancer, parathyroid cancer, and pituitary cancer.

[0097] Additional cancers that the compounds described herein may be useful in preventing, treating, and studying are, for example, colon carcinoma, familiary adenomatous polyposis carcinoma, and hereditary non-polyposis colorectal cancer, or melanoma. Further, cancers include, but are not limited to, labial carcinoma, larynx carcinoma, hypopharynx carcinoma, tongue carcinoma, salivary gland carcinoma, gastric carcinoma, adenocarcinoma, thyroid cancer (medullary and papillary thyroid carcinoma), renal carcinoma, kidney parenchyma carcinoma, cervix carcinoma, uterine corpus carcinoma, endometrium carcinoma, chorion carcinoma, testis carcinoma, urinary carcinoma, melanoma, brain tumors such as glioblastoma, astrocytoma, meningioma, medulloblastoma and peripheral neuroectodermal tumors, gall bladder carcinoma, bronchial carcinoma, multiple myeloma, basalioma, teratoma, retinoblastoma, choroidea melanoma, seminoma, rhabdomyosarcoma, craniopharyngeoma, osteosarcoma, chondrosarcoma, myosarcoma, liposarcoma, fibrosarcoma, Ewing's sarcoma, and plasmocytoma.

[0098] "IKZF2-dependent disease or disorder" means any disease or disorder which is directly or indirectly affected by the modulation of IKZF2 protein levels.

[0099] "IKZF4-dependent disease or disorder" means any disease or disorder which is directly or indirectly affected by the modulation of IKZF4 protein levels.D. Specific Embodiments and Methods for Testing Compounds of Formula (I')

[0100] The present invention relates to compounds or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, or tautomers thereof, capable of modulating IKZF2 protein levels, which are useful for the treatment of diseases and disorders associated with modulation of IKZF2 protein levels. The disclosure further relates to compounds, or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, or tautomers thereof, which are useful for reducing or decreasing IKZF2 protein levels.

[0101] In one embodiment, the compounds of Formula (I') have the structure of Formula (I): or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0102] In one embodiment, the compounds of Formula (I') or Formula (I) have the structure of Formula (Ia): or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0103] In one embodiment, the compounds of Formula (I') or Formula (I) have the structure of Formula (Ib): or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0104] In another embodiment, the compounds of Formula (I') or Formula (I) have the structure of or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0105] In another embodiment, the compounds of Formula (I') or Formula (I) have the structure of or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, and tautomers thereof.

[0106] In some embodiments of the formulae above (i.e., Formula (I'), Formula (I), Formula (Ia), Formula (lb), Formula (Ic), and / or Formula (Id)), R 2 is (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, -C(O)O(CH 2 ) 0-3 (C 6 -C 10 )aryl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl are optionally substituted with one to four R 5 ; or R 1 and R 2 , when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heterocycloalkyl ring; each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 4 R 6' , -NR 6 C(O)R 6' , halogen, -OH, - NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 4 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 ; each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to four R 10 ; each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -(CH 2 ) 0-3 C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , - NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, adamantyl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one to four R 11, and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one to four substituents each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy,or two R 7 together with the carbon atom to which they are attached form a =(O), or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 7 together with the atoms to which they are attached form a (C 5 -C 7 ) cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 ; and each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy,(C 6 -C 10 )aryl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one to four substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy,(C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, - NH 2 , and CN.

[0107] In some embodiments of the formulae above, R 2 is (C 1 -C 6 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to four R 5 ; each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , -NR 6 C(O)R 6' , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 ; each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C6-C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to four R 10 ; and each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 .

[0108] In some embodiments of the formulae above, X 1 is CR 3 . In another embodiment, X 1 is CH.

[0109] In some embodiments of the formulae above, X 1 is CR 3 , R 3 is absent, and is a double bond.

[0110] In another embodiment, X 1 is CR 3 and is a single bond

[0111] In some embodiments of the formulae above, R x is H. In another embodiment, R x is D.

[0112] In some embodiments of the formulae above, each R 1 is independently (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, (C 1 -C 4 )hydroxyalkyl, or halogen. In another embodiment, each R 1 is independently (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, or (C 1 -C 4 )hydroxyalkyl. In yet another embodiment, each R 1 is independently (C 1 -C 4 )alkyl, (C 1 -C 4 )hydroxyalkyl, or halogen. In another embodiment, each R 1 is independently (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, or halogen. In yet another embodiment, each R 1 is independently (C 1 -C 4 )alkyl or (C 1 -C 4 )haloalkyl. In another embodiment, each R 1 is independently (C 1 -C 4 )alkyl or (C 1 -C 4 )hydroxyalkyl. In yet another embodiment, each R 1 is independently (C 1 -C 4 )alkyl or halogen. In another embodiment, each R 1 is independently (C 1 -C 3 )alkyl. In yet another embodiment, each R 1 is independently methyl, ethyl, or n-propyl, isopropyl. In another embodiment, each R 1 is independently methyl or ethyl. In another embodiment, each R 1 is independently methyl.

[0113] In some embodiments of the formulae above, two R 1 together with the carbon atoms to which they are attached form a 5-membered heterocycloalkyl ring. In another embodiment, two R 1 together with the carbon atoms to which they are attached form a 6-membered heterocycloalkyl ring.

[0114] In some embodiments of the formulae above, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a phenyl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring. In another embodiment, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a phenyl ring. In yet another embodiment, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a 5-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S. In yet another embodiment, two R 1 , when on adjacent atoms, together with the atoms to which they are attached form a 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S.

[0115] In some embodiments of the formulae above, R 2 is H, (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, - C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, -C(O)O(C 6 -C 10 )aryl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to four R 5 . In another embodiment, R 2 is H, (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to four R 5 .

[0116] In another embodiment, R 2 is (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, - C(O)O(CH 2 ) 0-3 (C 6 -C 10 )aryl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to four R 5 . In yet another embodiment, R 2 is (C 1 -C 6 )alkyl, -C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, -C(O)O(CH 2 ) 0-3 (C 6 -C 10 )aryl, (C 6 -C 10 )aryl, or 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to four R 4 ; and the aryl and heteroaryl are optionally substituted with one to four R 5 . In another embodiment, R 2 is (C 1 -C 6 )alkyl, - C(O)(C 1 -C 6 )alkyl, -C(O)(CH 2 ) 0-3 (C 6 -C 10 )aryl, or -C(O)O(CH 2 ) 0-3 (C 6 -C 10 )aryl, wherein the alkyl is optionally substituted with one to four R 4 .

[0117] In another embodiment, R 2 is H, (C 1 -C 4 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to three R 4 ; and wherein the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to three R 5 .

[0118] In another embodiment, R 2 is H, (C 1 -C 6 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is optionally substituted with one to three R 4 ; and wherein the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to three R 5 . In another embodiment, R 2 is H or (C 1 -C 6 )alkyl optionally substituted with one to four R 4 . In yet another embodiment, R 2 is H or (C 1 -C 6 )alkyl substituted with one to three R 4 . In another embodiment, R 2 is (C 1 -C 6 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is substituted with one to four R 4 ; and wherein the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to four R 5 . In yet another embodiment, R 2 is (C 1 -C 6 )alkyl, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the alkyl is substituted with one to three R 4 ; and wherein the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to three R 5 .

[0119] In another embodiment, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In yet another embodiment, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 . In yet another embodiment, R 2 is (C6-C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C 3 -C 8 )cycloalkyl, wherein the aryl, heteroaryl, and cycloalkyl are optionally substituted with one to three R 5 . In another embodiment, R 2 is (C 6 -C 10 )aryl, or (C 3 -C 8 )cycloalkyl, wherein the aryl, and cycloalkyl are optionally substituted with one to three R 5 . In yet another embodiment, R 2 is phenyl, or (C 3 -C 8 )cycloalkyl, wherein the phenyl, and cycloalkyl are optionally substituted with one to three R 5 . In another embodiment, R 2 is (C 1 -C 3 )alkyl optionally substituted with one to three R 4 . In yet another embodiment, R 2 is (C 1 -C 3 )alkyl substituted with one to three R 4 .

[0120] In another embodiment, R 2 is (C 3 -C 8 )cycloalkyl wherein the cycloalkyl is optionally substituted with one to three R 5 . In yet another embodiment, R 2 is (C 6 -C 10 )arylor 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl are optionally substituted with one to three R 5 . In another embodiment, R 2 is (C 6 -C 10 )aryloptionally substituted with one to three R 5 . In yet another embodiment, R 2 is 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 5 . In another embodiment, R 2 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 5 .

[0121] In some embodiments of the formulae above, R 1 and R 2 , when on adjacent atoms, together with the atoms to which they are attached form a 5-membered heterocycloalkyl ring. In another embodiment, R 1 and R 2 , when on adjacent atoms, together with the atoms to which they are attached form a 6-membered heterocycloalkyl ring.

[0122] In some embodiments of the formulae above, R 3 is H. In another embodiment, R 3 is absent when is a double bond.

[0123] In some embodiments of the formulae above, each R 4 is independently selected from -C(O)OR 6 , - C(O)NR 6 R 6' , -NR 6 C(O)R 6' , halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 4 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 . In another embodiment, each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , -NR 6 C(O)R 6' , halogen, -OH, -NH 2 , or CN. In another embodiment, each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , -NR 6 C(O)R 6' , halogen, or -OH. In another embodiment, each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 4 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 . In another embodiment, each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 .

[0124] In another embodiment, each R 4 is independently selected from -C(O)OR 6 , -C(O)NR 6 R 6' , and - NR 6 C(O)R 6' . In another embodiment, each R 4 is independently selected from -C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 . In yet another embodiment, each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to four R 7 . In another embodiment, each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0125] In another embodiment, each R 4 is independently selected from (C 6 -C 10 )aryland 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl are optionally substituted with one to three R 7 . In yet another embodiment, each R 4 is independently selected from (C 6 -C 10 )aryl and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl are substituted with one to three R 7 .

[0126] In another embodiment, each R 4 is independently selected from (C 3 -C 8 )cycloalkyl and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the cycloalkyl and heterocycloalkyl groups are optionally substituted with one to three R 7 . In another embodiment, each R 4 is independently selected from (C 3 -C 8 )cycloalkyl and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the cycloalkyl and heterocycloalkyl groups are substituted with one to three R 7 .

[0127] In another embodiment, each R 4 is independently (C 6 -C 10 )aryloptionally substituted with one to three R 7 . In yet another embodiment, each R 4 is independently 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 7 .

[0128] In another embodiment, each R 4 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 7 . In another embodiment, each R 4 is independently 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 7 .

[0129] In some embodiments of the formulae above, each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In yet another embodiment, each R 5 is independently selected from (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0130] In another embodiment, each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 3 -C 7 )cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0131] In another embodiment, each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )haloalkoxy. In yet another embodiment, each R 5 is independently selected from (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In another embodiment, each R 5 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy,(C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, and CN.

[0132] In some embodiments of the formulae above, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to four R 10 . In another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 10 , or two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one three R 10 .

[0133] In another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 10 . In yet another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one three R 10 .

[0134] In another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring optionally substituted with one to three R 10 . In yet another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 10 .

[0135] In another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring optionally substituted with one three R 10 . In yet another embodiment, two R 5 , when on adjacent atoms, together with the atoms to which they are attached form a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one three R 10 .

[0136] In some embodiments of the formulae above, R 6 is H or (C 1 -C 3 )alkyl. In another embodiment, R 6 is H or (C 6 -C 10 )aryl. In yet another embodiment, R 6 is (C 1 -C 3 )alkyl or (C 6 -C 10 )aryl. In another embodiment, R 6 is H, methyl, ethyl, n-propyl, or isopropyl. In another embodiment, R 6 is H, methyl or ethyl. In yet another embodiment, R 6 is H or methyl. In another embodiment, R 6 is H.

[0137] In some embodiments of the formulae above, R 6' is H or (C 1 -C 3 )alkyl. In another embodiment, R 6' is H or (C 6 -C 10 )aryl. In yet another embodiment, R 6' is (C 1 -C 3 )alkyl or (C 6 -C 10 )aryl. In another embodiment, R 6' is H, methyl, ethyl, n-propyl, or isopropyl. In another embodiment, R 6' is H, methyl or ethyl. In yet another embodiment, R 6' is H or methyl. In another embodiment, R 6' is H.

[0138] In some embodiments of the formulae above, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , - (CH 2 ) 0-3 C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , -NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, adamantyl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one to four R 11 , and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one to four substituent each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy. In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -(CH 2 ) 0-3 C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , -NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one to four R 11, and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one to four substituent each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy.

[0139] In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)F 8 , -(CH 2 ) 0-3 C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , -NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 6 -C 10 )aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one to four R 11, and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one to four substituent each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy.

[0140] In another embodiment, each R 7 is independently selected from -(CH 2 ) 0-3 C(O)OR 8 , -NR 8 C(O)OR 9 , -S(O) p NR 8 R 9 , -S(O) p R 12 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -O(CH 2 ) 1-3 CN, -NH 2 , CN, -O(CH 2 ) 0-3 (C 6 -C 10 )aryl, -O(CH 2 ) 0-3 -5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, bicyclic 9- or 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl and heterocycloalkyl are optionally substituted with one or more substituent each independently selected from halogen, (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and (C 1 -C 6 )alkoxy.

[0141] In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)R 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , - NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)R 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN.

[0142] In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)R 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In yet another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy. In another embodiment, each R 7 is independently selected from -C(O)R 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In another embodiment, each R 7 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0143] In another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, -C(O)R 8 , -C(O)OR 8 , -C(O)NR 8 R 9 , -NR 8 C(O)R 9 , (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , CN, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 7 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S. In yet another embodiment, each R 7 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, halogen, -OH, CN, and (C 6 -C 10 )aryl.

[0144] In some embodiments of the formulae above, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 . In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring optionally substituted with one or more R 10 . In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 . In another embodiment, two R 7 together with the atoms to which they are attached form a (C 5 -C 7 ) cycloalkyl ring optionally substituted with one or more R 10 . In another embodiment, two R 7 together with the atoms to which they are attached form a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R 10 .

[0145] In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 6 -C 10 )aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 , or two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 .

[0146] In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 . In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a (C 5 -C 7 )cycloalkyl ring optionally substituted with one to four R 10 .In another embodiment, two R 7 , when on adjacent atoms, together with the atoms to which they are attached form a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to four R 10 .

[0147] In some embodiments of the formulae above, R 8 is H or (C 1 -C 3 )alkyl. In another embodiment, R 8 is H, methyl, ethyl, n-propyl, or isopropyl. In another embodiment, R 8 is H, methyl or ethyl. In yet another embodiment, R 8 is H or methyl. In another embodiment, R 8 is H

[0148] In some embodiments of the formulae above, R 9 is H or (C 1 -C 3 )alkyl. In another embodiment, R 9 is H, methyl, ethyl, n-propyl, or isopropyl. In another embodiment, R 9 is H, methyl or ethyl. In yet another embodiment, R 9 is H or methyl. In another embodiment, R 9 is H.

[0149] In some embodiments of the formulae above, each R 10 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, and halogen. In another embodiment, each R 10 is independently selected from -OH, -NH 2 , and CN. In yet another embodiment, each R 10 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, and halogen. In another embodiment, each R 10 is independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, and halogen. In yet another embodiment, each R 10 is independently selected from (C 1 -C 6 )alkyl and halogen.

[0150] In some embodiments of the formulae above, two R 10 together with the carbon atom to which they are attached form a =(O).

[0151] In some embodiments of the formulae above, each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy, (C 6 -C 10 )aryl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one to four substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In another embodiment, each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy, (C 6 -C 10 )aryl, and 5-to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one to three substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, -NH 2 , and CN. In yet another embodiment, each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy, and (C 6 -C 10 )aryl, wherein the aryl is optionally substituted with one to three substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, - NH 2 , and CN.

[0152] In another embodiment, each R 11 is independently selected from CN, (C 1 -C 6 )alkoxy, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the heterocycloalkyl is optionally substituted with one to four substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, - NH 2 , and CN. In another embodiment, each R 11 is independently selected from CN and (C 1 -C 6 )alkoxy. In yet another embodiment, each R 11 is independently selected from (C 6 -C 10 )aryl and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one to four substituents each independently selected from (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 1 -C 6 )haloalkyl, (C 1 -C 6 )haloalkoxy, (C 1 -C 6 )hydroxyalkyl, halogen, -OH, - NH 2 , and CN.

[0153] In some embodiments of the formulae above, R 12 is (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, (C 6 -C 10 )aryl, or 5- or 6-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, R 12 is (C 1 -C 6 )alkyl, (C 1 -C 6 )haloalkyl, phenyl, or 5- or 6-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S. In another embodiment, R 12 is (C 1 -C 4 )alkyl, (C 1 -C 4 )haloalkyl, phenyl, or 5- or 6-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0154] In some embodiments of the formulae above, p is 0 or 1. In another embodiment, p is 1 or 2. In yet another embodiment, p is 0 or 2. In another embodiment, p is 0. In yet another embodiment, p is 1. In another embodiment, p is 2.

[0155] In some embodiments of the formulae above, n is 0 or 1. In another embodiment, n is 1 or 2. In yet another embodiment, n is 0 or 2. In another embodiment, n is 0. In yet another embodiment, n is 1. In another embodiment, n is 2.

[0156] In some embodiments of the formulae above, n + n1 ≤ 3.

[0157] In some embodiments of the formulae above, n1 is 1. In another embodiment, n1 is 2.

[0158] In some embodiments of the formulae above, n is 0 and n1 is 1. In another embodiment, n is 1 and n1 is 2. In another embodiment, n is 2 and n1 is 1. In another embodiment, n is 1 and n1 is 1.

[0159] In some embodiments of the formulae above, q is 0, 1, 2, or 3. In another embodiment, q is 1, 2, 3, or 4. In yet another embodiment, q is 0, 1, or 2. In another embodiment, q is 1, 2, or 3. In yet another embodiment, q is 2, 3, or 4. In another embodiment, q is 0 or 1. In yet another embodiment, q is 1 or 2. In another embodiment, q is 2 or 3. In yet another embodiment, q is 3 or 4. In another embodiment, q is 0. In yet another embodiment, q is 1. In another embodiment, q is 2. In yet another embodiment, q is 3. In another embodiment, q is 4.

[0160] In some embodiments of the formulae above, X 1 is CH and n is 1. In another embodiment, X 1 is CH, n is 1, and q is 0.

[0161] In some embodiments of the formulae above, X 1 is CH, n is 1, and q is 0 or 1. In another embodiment, X 1 is CH, n is 1, q is 0 or 1, and R 1 is (C 1 -C 6 )alkyl. In another embodiment, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In another embodiment, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 .

[0162] In another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 .

[0163] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from - C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0164] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from -C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0165] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0166] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5-or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0167] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, cycloalkyl, and heterocycloalkyl are optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0168] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, and R 2 is (C 6 -C 10 )aryl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 5 .

[0169] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, cycloalkyl, and heterocycloalkyl are optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0170] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 1, q is 0, and R 2 is 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 1, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 5 .

[0171] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, and R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In another embodiment X 1 is CH, n is 1, q is 0, and R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 .

[0172] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from -C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0173] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from -C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0174] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5-or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0175] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5-to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0176] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0177] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, (C 6 -C 10 )aryl, 5-or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0178] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0179] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0180] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5-to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0181] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0182] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0183] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from halogen, -OH, phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5-to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0184] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0185] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0186] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5-to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0187] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from phenyl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0188] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from phenyl and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl groups are optionally substituted with one to three R 7 .

[0189] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from phenyl and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl groups are optionally substituted with one to three R 7 .

[0190] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from phenyl and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl groups are optionally substituted with one to three R 7 .

[0191] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from phenyl and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heteroaryl groups are optionally substituted with one to three R 7 .

[0192] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is phenyl optionally substituted with one to three R 7 .

[0193] In some embodiments of the formulae above, X 1 is CH, n is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is phenyl optionally substituted with one to three R 7 .

[0194] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is phenyl optionally substituted with one to three R 7 .

[0195] In some embodiments of the formulae above, X 1 is CH, n is 1, n1 is 1, q is 0, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is phenyl optionally substituted with one to three R 7 .

[0196] In some embodiments of the formulae above, X 1 is CH and n is 2. In another embodiment, X 1 is CH, n is 2, and q is 0. In yet another embodiment, X 1 is CH, n is 2, and q is 0 or 1. In another embodiment, X 1 is CH, n is 2, q is 0 or 1, and R 1 is (C 1 -C 6 )alkyl.

[0197] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In another embodiment, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 .

[0198] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0, and R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 . In another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 .

[0199] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from - C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0200] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from -C(O)OR 6 , (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0201] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl optionally substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0202] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, R 2 is (C 1 -C 6 )alkyl substituted with one to three R 4 , and each R 4 is independently selected from (C 6 -C 10 )aryl, 5-or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C 3 -C 8 )cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one to three R 7 .

[0203] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, cycloalkyl, and heterocycloalkyl are optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0204] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0, and R 2 is (C 6 -C 10 )aryl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 5 .

[0205] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, cycloalkyl, and heterocycloalkyl are optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl, (C 3 -C 8 )cycloalkyl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S.

[0206] In some embodiments of the formulae above, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 6 -C 10 )aryl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 2, q is 0, and R 2 is 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one to three R 5 . In yet another embodiment, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is (C 3 -C 8 )cycloalkyl optionally substituted with one to three R 5 . In another embodiment, X 1 is CH, n is 2, q is 0 or 1, R 1 is (C 1 -C 6 )alkyl, and R 2 is 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one to three R 5 .

[0207] In some embodiments of the formulae above,

[0208] In some embodiments of the formulae above,

[0209] In some embodiments of the formulae above,

[0210] Non-limiting illustrative compounds of the disclosure include: Cmpd No. Structure Compound Name I-1 3-(5-(1-ethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-2 3-(1-oxo-5-(1-propylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-3 3-(5-(1-(cyclopropylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-4 3-(5-(1-isobutylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-5 3-(5-(1-(cyclobutylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-6 3-(5-(1-(oxazol-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-7 3-(1-oxo-5-(1-(thiazol-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-8 3-(5-(1-(cyclopentylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-9 3-(5-(1-((5-chlorothiophen-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-10 3-(5-(1-((2-chlorothiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-11 3-(5-(1-(cyclohexylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-12 3-(1-oxo-5-(1-(2-(pyrrolidin-1-yl)ethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-13 3-(1-oxo-5-(1-((tetrahydro-2H-pyran-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-14 3-(1-oxo-5-(1-phenethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-15 3-(5-(1-(3-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-16 3-(5-(1-(3-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-17 3-(5-(1-(2-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-18 3-(5-(1-(2-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-19 3-(1-oxo-5-(1-(2-(piperidin-1-yl)ethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-20 3-(5-(1-((3,5-dimethylisoxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-21 3-(5-(1-((1,3-dimethyl-1H-pyrazol-5-yl)methyl)pipendin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-22 3 -(5-(1-((6-methylpyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-23 3-(5-(1-(3-morpholinopropyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-24 3-(5-(1-(2,6-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-25 3-(5-(1-(2,6-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-26 3-(5-(1-(3,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-27 3-(5-(1-(3,5-dibromobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-y l)piperidine-2,6-dioneI-28 3-(5-(1-(3-chloro-5-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-29 3-(5-(1-(2,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-30 3-(5-(1-(2,5-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-31 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl) benzonitrile (or 3-(5-(1-(4-nitrilebenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione)I-32 3-(5-(1-(4-(hydroxymethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-33 3-(5-(1-(3,4-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-34 3-(5-(1-(4-chloro-2-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-35 3-(5-(1-(2-chloro-4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-36 3 -((4-(2-(2,6-dioxopiperidin-3 - yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl) benzonitrileI-37 3-(5-(1-(2,3-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-38 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1 -oxoisoindolin-5 - yl)piperidin-1-yl)methyl) benzonitrileI-39 3-(5-(1-(4-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-40 3-(5-(1-(2,5-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-41 3-(5-(1-(3,4-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-42 3-(5-(1-(2,4-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-43 3-(5-(I-((IH-indazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-44 3-(5-(1-((1H-benzo[d]imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-45 3-(5-(1-(4-isopropylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-46 methyl 5-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1 - yl)methyl)furan-2-carboxylateI-47 3-(5-(1-(naphthalen-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-48 3-(1-oxo-5-(1-(quinolin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-49 3-(5-(1-(naphthalen-1-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-50 3-(5-(1-((1-methyl-1H-benzo[d]imidazol-2-yl)methyl)piperidin-4-yl)-1 - oxoisoindolin-2-yl)piperidine-2,6-dioneI-51 3-(1-oxo-5-(1-(4-(trifluoromethoxy)benzyl)piperidi n-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-52 3 -(5 -(I-(4-(IH-pyrrol- I - yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-53 3 -(5 -(1 -(4-(1H-1,2,4-triazol-1 - yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-54 3-(1-oxo-5-(1-(3-(trifluoromethoxy)benzyl)piperidi n-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-55 3-(1-oxo-5-(1-(2-(trifluoromethoxy)benzyl)piperidi n-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-56 3 -(1 -oxo-5 -(1-((3-phenyl-1,2,4-oxadiazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-57 3-(5-(1-benzylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-58 3 -(1 -oxo-5 -(1 -(pyridin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-59 3-(1-oxo-5-(1-(pyridin-3-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-60 3-(1-oxo-5-(1-(pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-61 3-(1-oxo-5-(1-(pyrimidin-5-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-62 3-(1-oxo-5-(1-(1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-63 3-(5-(1-(4-(fluoromethyl)benzyl)piperidin-4-yl)-1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-64 3-(5-(1-(3,4-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-65 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1 -oxoisoindolin-5 - yl)piperidin-1-yl)methyl)pyrimidine- 5 - carbonitrileI-66 3 -(5-(1-(4-ethylbenzyl)piperidin-4-yl)- 1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-67 3-(5-(1-(2-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-68 3-(5-(1-((2-methoxypyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-69 3-(5-(1-(3-fluoro-4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-70 3-(5-(1-(4-(difluoromethyl)benzyl)piperidin-4-yl)- 1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-71 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1 -oxoisoindolin-5 - yl)piperidin-l-yl)methyl)benzamideI-72 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 - yl)piperidin- 1 -yl)methyl)benzoic acidI-73 3-(5-(1-(3-(difluoromethyl)benzyl)piperidin-4-yl)- 1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-74 3 -((4-(2-(2,6-dioxopiperidin-3 - yl)-1-oxoisoindolin-5 - yl)piperidin- 1 -yl)methyl)benzoic acidI-75 3-(1-oxo-5-(1-(4-propylbenzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-76 3-(1-oxo-5-(1-(4-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-77 3-(5-(1-(4-(difluoromethoxy)benzyl)piperidi n-4-yl)- 1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-78 3-(l-oxo-5-(l-((5-(trifluoromethyl)pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-79 3-(5-(1-(3-(difluoromethoxy)benzyl)piperidi n-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-80 3-(5-(1-(2-(difluoromethoxy)benzyl)piperidi n-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-81 3-(5-(1-(4-cyclobutylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-82 3-(5-(1-((2,3-dihydrobenzo[b]1,4]dioxin-5-yl)methyl)piperidin-4-yl)-1- oxoisoindolin-2-yl)piperidine-2,6-dioneI-83 3-(5-(1-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)methyl)piperidin-4-yl)-1 - oxoisoindolin-2-yl)piperidine-2,6-dioneI-84 3-(5-(1-(4-(tert-butyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-85 3-(5-(1-(4-isobutylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-86 N-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetamideI-87 3-(5-(1-((2,2-difluorobenzo[d][1,3]dioxol-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-88 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4] dioxepin-7 - yl)methyl)piperidin-4-yl)-1 - oxoisoindolin-2-yl)piperidine-2,6-dioneI-89 3-(1-oxo-5-(1-(4-(tert-pentyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione Cmpd No. Structure Compound Name I-90 3-(5-(1-([1,1'-biphenyl]-4-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-91 3-(5-(1-(4-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-92 3-(5-(1-(4-(1H-imidazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-93 3-(5-(1-(3-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-94 3-(5-(1-(4-cyclohexylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-95 3-(1-oxo-5-(1-(pyrimidin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-96 3-(5-(1-(4-bromobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-97 3-(5-(1-(4-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-98 3-(5-(1-(3,5-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-99 3-(5-(1-(4-chloro-3-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-100 3-(5-(1-(3-chloro-4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-101 3-(5-(1-(2,4-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-102 3-(5-(1-(3-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-103 3-(5-(1-(benzo[c][1,2,5]oxadiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-104 3-(5-(1-(2-cyclopropylbenzyl)piperidin-4-yl)-1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-105 3-(5-(1-((1,3-dihydroisobenzofuran-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-106 3-(1-oxo-5-(1-(2-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-107 3-(5-(1-(3-(tert-butyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-108 3-(5-(1-(3-isopropoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-109 3-(1-oxo-5-(1-(4-(thiophen-3-yl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-110 3-(5-(1-(4-cyclopentylbenzyl)piperidin-4-yl)-1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-111 3-(1-oxo-5-(1-(4-(pyrrolidin-1-yl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-112 3-(5-(1-(4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-113 3-(5-(1-(2,4-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-114 3-(1-oxo-5-(1-(quinolin-8-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-115 3-(5-(1-((1-methyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-116 3-(5-(1-((1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-117 3-(5-(1-((1-methyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-118 3-(5-(1-((1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-119 3-(5-(1-((1H-pyrrol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-120 3-(5-(1-((1H-imidazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-121 3-(5-(1-((1-ethyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-122 3-(5-(1-((2-aminopyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-123 3-(5-(1-((6-aminopyridin-3-yl)methyl)piperidin-4-yl)-1 - oxoisoindolin-2-yl)piperidine-2,6-dioneI-124 3 -(5 -(1-((5 -amino- I -methyl- I H-pyrazol-4-yl)methyl)piperidin-4-yl)-1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-125 3-(5-(1-((6-methylimidazo[2,1-b]thiazol-5-yl)methyl)piperidin-4-yl)-1 -oxoisoindolin-2-yl)piperidine-2,6-dioneI-126 3-(5-(1-(imidazo[1,2-a]pyrazin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-127 3-(5-(1-([1,2,4]triazolo[1,5-a]pyridin-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-128 3-(1-oxo-5-(1-(pyrazolo[1,5-a]pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-129 3-(5-(1-((1,4-dimethyl-1H-imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-130 3-(5-(1-(benzo [d]thiazol-5 - ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-131 3-(1-oxo-5-(1-(pyrazolo[1,5-a]pyrimidin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-132 3-(5-(1-(imidazo[1,2-a]pyrimidin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-133 3-(5-(1-(imidazo[1,2-a]pyrimidin-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-134 3-(5-(1-((1-cyclobutyl-1H-1,2,3-triazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-135 3-(1-oxo-5-(1-((4,5,6,7-tetrahydropyrazolo[1,5-a]pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-136 3-(5-(1-((1H-indol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-137 3-(5-(1-((1H-indazol-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-138 3-(5-(1-((1H-pyrrolo[2,3-b]pyridin-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-139 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzamideI-140 3-(5-(1-((1H-pyrrolo[2,3-b]pyridin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-141 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4]thiazin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-142 3-(1-oxo-5-(1-((2-(pyrrolidin-1-yl)pyrimidin-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-143 3-(5-(1-((2-(tert-butyl)thiazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-144 3-(1-oxo-5-(1-((2-(thiophen-2-yl)thiazol-5 -yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-145 3-(5-(1-((2-cyclohexylthiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-146 3-(5-(1-((5-cyclopropyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-147 3-(5-(1-((2-morpholinopyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-148 3-(1-oxo-5-(1-((3-phenyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-149 3-(5-(1-((6-methyl-1H-indol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-150 methyl 4-((4-(2-(2,6-dioxopiperidin-3-yl)-I-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-1H-pyrrole-2-carboxylateI-151 3-(1-oxo-5-(1-((3-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-152 3-(1-oxo-5-(1-((2-phenyl-1H-imidazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-153 3-(1-oxo-5-(1-((5-(pyridin-2-yl)-1H-pyrazol-3-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-154 3-(1-oxo-5-(1-((4-phenyl-1H-imidazol-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-155 3-(1-oxo-5-(piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-156 3-(5-(1-(3,5-difluoro-4-hydroxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-157 3-(5-(1-(2-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-158 3-(5-(1-(4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-159 3-(5-(1-(3,5-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-160 3-(5-((2S)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-161 3-(5-((2R)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-162 3-(5-(1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-163 3-(5-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-164 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-165 3-(5-(azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-166 3-(5-((R)-azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-167 3-(5-((S)-azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-168 3-(1-oxo-5-(1-((1,2,3,4-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-169 methyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetateI-170 3-(1-oxo-5-(1-phenylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-171 3-(1-oxo-5-(2,2,6,6-tetramethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-172 3-(5-(1-benzyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-173 3-(5-(1-(3-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-174 3-(5-(1-(2,6-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-175 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen- 2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-176 ethyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetateI-177 tert-butyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetateI-178 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetic acidI-179 3-(1-oxo-5-(1-(3,3,3-trifluoropropyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-180 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)-N-phenylacetamideI-181 3-(5-(1-(3-fluoropropyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-182 tert-butyl 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoateI-183 3-(5-(2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-184 3-(5-(3,3-dimethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-185 3-(5-(1-benzyl-3,3-dimethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-186 5-(3-methylpiperidin-4-yl)-2-(2-oxopiperidin-3-yl)isoindolin-1-oneI-187 3-(5-(1-benzyl-3-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-188 3-(5-(8-azabicyclo[3.2.1]octan-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-189 3-(5-(1-(2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-190 3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-191 3-(5-(1-benzyl-2,5-dihydro-1H-pyrrol-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-192 3-(5-(1-benzyl-2-oxo-1,2-dihydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-193 3-(5-(1-benzyl-2-oxopiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-194 3-(1-oxo-5-(2-oxopiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-195 3-(1-oxo-5-(2-oxo-1,2-dihydropyridin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-196 3-(1-oxo-5-(1,2,3,4-tetrahydroquinolin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-197 3-(5-(1-benzyl-1,2,3,4-tetrahydroquinolin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-198 3-(5-(1-((1-benzyl-1H-tetrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-199 3-(1-oxo-5-(1-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-200 3-(5-(1-(benzo[d]thiazol-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-201 3-(1-oxo-5-(1-((3-(pyridin-2-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-202 3-(5-(1-((R)-2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-203 3-(5-(1-((1-methyl-1H-indazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-204 3-(5-(1-((1,2,4-oxadiazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-205 3-(5-(1-(4-hydroxy-3-((4-methylpiperazin-1-yl)methyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-206 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1 -oxoisoindolin-5 - yl)piperidin-1-yl)methyl)phenyl)acetonitrileI-207 3-(5-(1-((2-(4-chlorophenyl)-5 - methyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-208 3-(5-(1-((7-hydroxy-2-methylpyrazolo[1,5-a]pyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-209 3-(5-(1-(2,2-difluoro-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-210 3-(5-(1-((3-fluorobicyclo[1.1.1]pentan-1-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-211 3-(1-oxo-5-(1-((2-phenylthiazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-212 3-(5-(1-(2-fluoro-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-213 3-(1-oxo-5-(1-((4-oxo-3,4-dihydrothieno[3,2-d]pyrimidin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-214 3-(1-oxo-5-(1-(quinolin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-215 3-(5-(1-(3,5-bis(trifluoromethyl)benzyl)piperi din-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-216 3 -((4-(2-(2,6-dioxopiperidin-3 - yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-N,N-dimethylbenzenesulfonamideI-217 6-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl)picolinonitrileI-218 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1 -oxoisoindolin-5 - yl)piperidin-1-yl)methyl)phenoxy)acetonitrileI-219 3-(5-(1-((1H-indazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-220 3-(5-(1-(2,2-difluoroethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-221 3-(5-(1-((7-methyl-4-oxo-4H-pyrido[1,2-a]pyrimidin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-222 benzyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidine-1-carboxylateI-223 3-(1-oxo-5-(1-(2-phenylacetyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-224 3-(1-oxo-5-(1-(2,2,2-trifluoro-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-225 3-(5-(1-(4-(5-methylbenzo [d]thiazol-2-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-226 3-(5-(1-(isoquinolin-1-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-227 3-(5-(1-(4-(4-methoxypiperidin-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-228 3-(5-(1-(4-(isopropylthio)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-229 tert-butyl (5-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-4-(trifluoromethyl)thiazol-2-yl)carbamateI-230 3-(1-oxo-5-(1-((S)-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-231 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl)phenyl)acetic acidI-232 3-(5-(1-((7-fluoroquinolin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-233 3-(5-(1-((5-methyl-2-(4-(trifluoromethyl)phenyl)oxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-234 3-(5-(1-((2-amino-4-(trifluoromethyl)thiazol-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)pi peri dine-2,6-dioneI-235 3 -((4-(2-(2,6-dioxopiperidin-3 - yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl)-1,2,4-oxadiazole -5 -carboxamideI-236 3-(5-(1-(3-(morpholinosulfonyl)benzyl)pi per idin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-237 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl)-N,N-dimethylbenzene sulfonamideI-238 3-(1-oxo-5-(1-(thiazol-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-239 3-(1-oxo-5-(1-(quinoxalin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-240 3-(5-(1-((2-(4-fluorophenyl)-5-methyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)pi peri dine-2,6-dioneI-241 3-(1-oxo-5-(1-((3-(m-tolyl)-1,2,4-oxadiazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-242 3-(5-(1-(4-(tert-butyl)benzoyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-243 3-(1-oxo-5-(1-((5-(4-(trifluoromethyl)phenyl)-1,2,4-oxadiazol-3-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-244 3-(5-(1-(4-((4-fluorobenzyl)oxy)benzyl)piperidi n-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-245 3-(5-(1-((3 -methylisoxazol-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-246 3-(5-(1-(isoxazol-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-247 3-(1-oxo-5-(1-((R)-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-248 3-(5-(1-(4-(methoxymethyl)benzyl)piperidin -4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-249 3-(5-(1-((S)-2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-250 3-(1-oxo-5-(1-(phenylsulfonyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-251 3-(5-(1-((5-methyl-3-phenylisoxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-252 3-(5-(1-(4-((difluoromethyl)sulfonyl)benzyl) piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-253 3-(1-oxo-5-(1-(2,2,2-trifluoroethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-254 methyl 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)oxazole-4-carboxylateI-255 3-(1-oxo-5-(1-(4-(pyridin-2-ylmethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-256 3-(5-(1-acetylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-257 3-(5-(1-((5-methyl-2-phenyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-258 3-(5-(1-((3-cyclohexylisoxazol-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-259 3-(1-oxo-5-(1-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-260 3-(5-(1-benzylpyrrolidin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-261 (R)-3-(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-262 (S)-3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-263 3-(5-(1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-264 3-(5-(1-methyl-2,3,6,7-tetrahydro-1H-azepin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-265 3-(5-(8-benzyl-8-azabicyclo[3.2.1]octan-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-266 trans-3-(1-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)meth yl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-267 (S)-3-(1-oxo-5-((S)-piperidin-3-yl)isoindolin-2-yl)piperidine-2,6-dioneI-268 3-(5-(1-acetyl-1,2,5,6-tetrahydropyridin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-269 (R)-3-(5-((R)-1-acetylpyrrolidin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-270 3-(5-(1-acetyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-271 3-(5-(octahydroindolizin-7-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-272 (R)-3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-273 3-(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-274 3-(5-(2,5-dihydro-1H-pyrrol-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-275 3-(5-(1-acetyl-2,5-dihydro-1H-pyrrol-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-276 cis-3-(1-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)meth yl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-277 3-(1-oxo-5-(2,3,6,7-tetrahydro-1H-azepin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-278 3-(5-(1-methylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-279 (R)-3-(1-oxo-5 -((S)-piperidin-3 - yl)isoindolin-2-yl)piperidine-2,6-dioneI-280 3-(1-oxo-5-(1,2,3,6-tetrahydropyridin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-281 (S)-3 -(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-282 3-(1-oxo-5-(1,2,5, 6-tetrahydropyridin-3-yl)isoindolin-2-yl)piperidine-2,6-dioneI-283 3-(1-oxo-5-(2,2,6,6-tetramethyl-1,2,3,6-tetrahydropyridin-4-yl)isoindolin-2-yl)piperidine -2,6-dioneI-284 (S)-3-(5-((R)-1-acetylpyrrolidin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-285 3-(5-(1-((6-isopropoxypyridin-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-286 3-(1-oxo-5-(1-((1-phenyl-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-287 3-(5-(1-(4-ethoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-288 3-(1-oxo-5-(1-((1-phenyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-289 3-(5-(1-((1-isopropyl-1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)pi peri dine-2,6-dioneI-290 3-(5-(1-(isothiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-291 3-(5-(1-((1-isopropyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-292 3-(5-(1-((1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-293 3-(5-(1-((5-isopropoxypyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-294 3-(1-oxo-5-(1-((1-(pyridin-3-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-295 3-(1-oxo-5-(1-((1-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dioneI-296 5 -((4-(2-(2,6-dioxopiperidin-3 - yl)-1-oxoisoindolin-5 - yl)piperidin-1-yl)methyl)-2-fluorobenzonitrileI-297 3-(5-(1-((5-fluoropyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-298 3-(5-(1-((1-ethyl-3 -(pyridin-3 -yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-299 3-(5-(1-((6-methoxypyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-300 3-(5-(1-((3-((3S,5 S)-adamantan-1-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-301 3-(5-(1-((6-isopropoxypyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-302 3-(5-(1-((1-benzyl-5-(pyridin-2-yl)-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dioneI-303 trans-3-(5-(1-((4-methoxycyclohexyl)methyl)piperi din-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione

[0211] In another embodiment of the disclosure, the compounds of the present disclosure are enantiomers. In some embodiments the compounds are the (S)-enantiomer. In other embodiments the compounds are the (R)-enantiomer. In yet other embodiments, the compounds of the present disclosure may be (+) or (-) enantiomers.

[0212] It should be understood that all isomeric forms are included within the present invention, including mixtures thereof. If the compound contains a double bond, the substituent may be in the E or Z configuration. If the compound contains a disubstituted cycloalkyl, the cycloalkyl substituent may have a cis- or trans configuration. All tautomeric forms are also intended to be included.

[0213] Compounds of the invention, and pharmaceutically acceptable salts, hydrates, solvates, and stereoisomers thereof may exist in their tautomeric form (for example, as an amide or imino ether). All such tautomeric forms are contemplated herein as part of the present disclosure.

[0214] The compounds of the invention may contain asymmetric or chiral centers and, therefore, exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds of the invention as well as mixtures thereof, including racemic mixtures, form part of the present invention. In addition, the present invention embraces all geometric and positional isomers. For example, if a compound of the invention incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention. Each compound herein disclosed includes all the enantiomers that conform to the general structure of the compound. The compounds may be in a racemic or enantiomerically pure form, or any other form in terms of stereochemistry. The assay results may reflect the data collected for the racemic form, the enantiomerically pure form, or any other form in terms of stereochemistry.

[0215] Diastereomeric mixtures can be separated into their individual diastereomers on the basis of their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and / or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chiral auxiliary such as a chiral alcohol or Mosher's acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. Also, some of the compounds of the invention may be atropisomers (e.g., substituted biaryls) and are considered as part of this invention. Enantiomers can also be separated by use of a chiral HPLC column.

[0216] It is also possible that the compounds of the invention may exist in different tautomeric forms, and all such forms are embraced within the scope of the invention and chemical structures and names. Also, for example, all keto-enol and imine-enamine forms of the compounds are included in the invention.

[0217] All stereoisomers (for example, geometric isomers, optical isomers, and the like) of the present compounds (including those of the salts, solvates, and esters of the compounds as well as the salts, solvates and esters of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this invention as are positional isomers (such as, for example, 4-pyridyl and 3-pyridyl). (For example, if a compound of Formula (I') or Formula (I) incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention. Also, for example, all keto-enol and imine-enamine forms of the compounds are included in the invention. Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or is admixed, for example, as racemates or with all other, or other selected, stereoisomers.

[0218] The chiral centers of the compounds of the invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations. In certain embodiments, each asymmetric atom has at least 50% enantiomeric excess, at least 60% enantiomeric excess, at least 70% enantiomeric excess, at least 80% enantiomeric excess, at least 90% enantiomeric excess, at least 95% enantiomeric excess, or at least 99% enantiomeric excess in the (R)- or (S)- configuration. Substituents at atoms with unsaturated double bonds may, if possible, be present in cis-(Z)- or trans-(E)- form.

[0219] The use of the terms "salt", "solvate", "ester," "prodrug", and the like, is intended to equally apply to the salt, solvate, ester, and prodrug of enantiomers, stereoisomers, rotamers, tautomers, positional isomers, racemates, or prodrugs of the inventive compounds.

[0220] The compounds of the invention may form salts which are also within the scope of this invention. Reference to a compound of the Formula herein is generally understood to include reference to salts thereof, unless otherwise indicated.

[0221] The compounds and intermediates may be isolated and used as the compound per se. Any formula given herein is also intended to represent unlabeled forms as well as isotopically labeled forms of the compounds. Isotopically labeled compounds have structures depicted by the formulas given herein except that one or more atoms are replaced by an atom having a selected atomic mass or mass number. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine, and, such as 2< H, 3< H, 11< C, 13< C, 14< C, 15< N, 18< F, 31< P, 32< P, respectively. The invention includes various isotopically labeled compounds as defined herein, for example those into which radioactive isotopes, such as 3< H, 13< C, and 14< C, are present. Such isotopically labelled compounds are useful in metabolic studies (with 14< C), reaction kinetic studies (with, for example 2< H or 3< H), detection or imaging techniques, such as positron emission tomography (PET) or single-photon emission computed tomography (SPECT) including drug or substrate tissue distribution assays, or in radioactive treatment of patients. In particular, an 18< F, 11< C or labeled compound may be particularly desirable for PET or SPECT studies.

[0222] Further, substitution with heavier isotopes, particularly deuterium (i.e., 2< H or D) may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life, reduced dosage requirements, reduced CYP450 inhibition (competitive or time dependent) or an improvement in therapeutic index. For example, substitution with deuterium may modulate undesirable side effects of the undeuterated compound, such as competitive CYP450 inhibition, time dependent CYP450 inactivation, etc. It is understood that deuterium in this context is regarded as a substituent in compounds of the present invention. The concentration of such a heavier isotope, specifically deuterium, may be defined by the isotopic enrichment factor. The term "isotopic enrichment factor" as used herein means the ratio between the isotopic abundance and the natural abundance of a specified isotope. If a substituent in a compound of this invention is denoted deuterium, such compound has an isotopic enrichment factor for each designated deuterium atom of at least 3500 (52.5% deuterium incorporation at each designated deuterium atom), at least 4000 (60% deuterium incorporation), at least 4500 (67.5% deuterium incorporation), at least 5000 (75% deuterium incorporation), at least 5500 (82.5% deuterium incorporation), at least 6000 (90% deuterium incorporation), at least 6333.3 (95% deuterium incorporation), at least 6466.7 (97% deuterium incorporation), at least 6600 (99% deuterium incorporation), or at least 6633.3 (99.5% deuterium incorporation).

[0223] Isotopically-labeled compounds of the present invention can generally be prepared by conventional techniques known to those skilled in the art or by carrying out the procedures disclosed in the schemes or in the examples and preparations described below using an appropriate isotopically-labeled reagent in place of the non-isotopically labeled reagent.

[0224] Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e.g., D 2 O, d 6 -acetone, d 6 -DMSO.

[0225] The present disclosure relates to compounds which are modulators of IKZF2 protein levels. In one embodiment, the compounds of the present disclosure decrease IKZF2 protein levels. In yet one embodiment, the compounds of the present disclosure reduce IKZF2 protein levels. In another embodiment, the compounds of the present disclosure are degraders of IKZF2.

[0226] The present disclosure relates to compounds which are modulators of IKZF2 and IKZF4 protein levels. In one embodiment, the compounds of the present disclosure decrease IKZF2 and IKZF4 protein levels. In yet one embodiment, the compounds of the present disclosure reduce IKZF2 and IKZF4 protein levels. In another embodiment, the compounds of the present disclosure are degraders of IKZF2.

[0227] In some embodiments, the compounds of the invention are selective over other proteins. As used herein "selective modulator", "selective degrader", or "selective compound" means, for example, a compound of the invention, that effectively modulates, decreases, or reduces the levels of a specific protein or degrades a specific protein to a greater extent than any other protein. A "selective modulator", "selective degrader", or "selective compound" can be identified, for example, by comparing the ability of a compound to modulate, decrease, or reduce the levels of or to degrade a specific protein to its ability to modulate, decrease, or reduce the levels of or to degrade other proteins. In some embodiments, the selectivity can be identified by measuring the EC 50 or IC 50 of the compounds.

[0228] In some embodiments, the compounds of the present invention are selective IKZF2 modulators. As used herein "selective IKZF2 modulator", "selective IKZF2 degrader", or "selective IKZF2 compound" refers to a compound of the invention, for example, that effectively modulates, decrease, or reduces the levels of IKZF2 protein or degrades IKZF2 protein to a greater extent than any other protein, particularly any protein (transcription factor) from the Ikaros protein family (e.g., IKZF1, IKZF3, IKZF4, and IKZF5).

[0229] A "selective IKZF2 modulator", "selective IKZF2 degrader", or "selective IKZF2 compound" can be identified, for example, by comparing the ability of a compound to modulate IKZF2 protein levels to its ability to modulate levels of other members of the Ikaros protein family or other proteins. For example, a substance may be assayed for its ability to modulate IKZF2 protein levels, as well as IKZF1, IKZF3, IKZF4, IKZF5, and other proteins. In some embodiments, the selectivity can be identified by measuring the EC 50 of the compounds. In some embodiments, a selective IKZF2 degrader is identified by comparing the ability of a compound to degrade IKZF2 to its ability to degrade other members of the Ikaros protein family or other proteins.

[0230] In certain embodiments, the compounds of the invention are IKZF2 degraders that exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over other proteins (e.g., IKZF1, IKZF3, IKZF4, and IKZF5). In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over other proteins.

[0231] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over the other members of the Ikaros protein family (e.g., IKZF1, IKZF3, IKZF4, and IKZF5). In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over the other members of the Ikaros protein family (e.g., IKZF1, IKZF3, IKZF4, and IKZF5).

[0232] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over IKZF1. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over IKZF1.

[0233] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over IKZF3. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over IKZF3.

[0234] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over IKZF4. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over IKZF4.

[0235] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 over IKZF5. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 over IKZF5.

[0236] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 and IKZF4 over the other members of the Ikaros protein family (e.g., IKZF1, IKZF3, and IKZF5). In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 and IKZF4 over the other members of the Ikaros protein family (e.g., IKZF1, IKZF3, and IKZF5).

[0237] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF1. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF1.

[0238] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF3. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF3.

[0239] In certain embodiments, the compounds of the invention exhibit at least 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold or 100-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF5. In various embodiments, the compounds of the invention exhibit up to 1000-fold selectivity for the degradation of IKZF2 and IKZF4 over IKZF5.

[0240] In some embodiments, the degradation of IKZF2 is measured by EC 50 .

[0241] Potency of can be determined by EC 50 value. A compound with a lower EC 50 value, as determined under substantially similar degradation conditions, is a more potent degrader relative to a compound with a higher EC 50 value. In some embodiments, the substantially similar conditions comprise determining degradation of protein levels in cells expressing the specific protein, or a fragment of any thereof.

[0242] The invention is directed to compounds as described herein and pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, or tautomers thereof, and pharmaceutical compositions comprising one or more compounds as described herein, or pharmaceutically acceptable salts, hydrates, solvates, stereoisomers, or tautomers thereof.E. Methods of Synthesizing Compounds of Formula (I')

[0243] The compounds of the present invention may be made by a variety of methods, including standard chemistry. Suitable synthetic routes are depicted in the Schemes given below.

[0244] The compounds of the present invention may be prepared by methods known in the art of organic synthesis as set forth in part by the following synthetic schemes. In the schemes described below, it is well understood that protecting groups for sensitive or reactive groups are employed where necessary in accordance with general principles or chemistry. Protecting groups are manipulated according to standard methods of organic synthesis (T.W. Greene and P.G.M. Wuts, "Protective Groups in Organic Synthesis", Third edition, Wiley, New York 1999). These groups are removed at a convenient stage of the compound synthesis using methods that are readily apparent to those skilled in the art. The selection processes, as well as the reaction conditions and order of their execution, shall be consistent with the preparation of Compounds of Formula (I').

[0245] Those skilled in the art will recognize if a stereocenter exists in the compounds of the present invention. Accordingly, the present invention includes both possible stereoisomers (unless specified in the synthesis) and includes not only racemic compounds but the individual enantiomers and / or diastereomers as well. When a compound is desired as a single enantiomer or diastereomer, it may be obtained by stereospecific synthesis or by resolution of the final product or any convenient intermediate. Resolution of the final product, an intermediate, or a starting material may be affected by any suitable method known in the art. See, for example, "Stereochemistry of Organic Compounds" by E.L. Eliel, S.H. Wilen, and L.N. Mander (Wiley-Interscience, 1994).

[0246] The compounds described herein may be made from commercially available starting materials or synthesized using known organic, inorganic, and / or enzymatic processes.Preparation of Compounds

[0247] The compounds of the present invention can be prepared in a number of ways well known to those skilled in the art of organic synthesis. By way of example, compounds of the present invention can be synthesized using the methods described below, together with synthetic methods known in the art of synthetic organic chemistry, or variations thereon as appreciated by those skilled in the art. Preferred methods include but are not limited to those methods described below.

[0248] Compounds of the present invention can be synthesized by following the steps outlined in General Schemes I, II, III, IV, and V which comprise different sequences of assembling intermediates I-a to I-p. Starting materials are either commercially available or made by known procedures in the reported literature or as illustrated. wherein X 1 is CR 3 , and R 1 , R 2 , R 3 , R x , n, n1, and q are as defined in Formula (I').

[0249] The general way of preparing Compounds of Formula (I') wherein X 1 is CH and R 2 is a substituted alkyl (optionally substituted with one or more R 4 ) by using intermediates I-a, I-b, I-c, I-d, I-e, I-f, and I-g is outlined in General Scheme I. Coupling of I-a with boronic ester I-b using a catalyst (e.g., Pd(dppf)Cl 2 •DCM), and a base (e.g., cesium carbonate (CS 2 CO 3 )), in a solvent (e.g., N,N-dimethylformamide (DMF)) at elevated temperature yields I-c. Hydrogenation of I-d in the presence of a suitable catalyst (e.g., Pd / C or PtO 2 ) in a solvent (e.g., DMF) and under an atmosphere of hydrogen gas provides I-d. When P is an amine protecting group (e.g., tert-butyloxycarbonyl (Boc)) intermediate I-d is deprotected using a strong acid such as trifluoroacetic acid (TFA) or hydrochloric acid (HCl) in a solvent (e.g., tetrahydrofuran (THF), 1,2,-dichloroethane, dioxane or dichloromethane (DCM)) optionally at elevated temperature to provide I-e. Reductive amination of I-e with aldehyde or ketone I-g provides a compound of Formula (I') where X 1 is CH and R 2 is a substituted alkyl. Alternatively, Compounds of Formula (I') where X 1 is CH and R 2 is a substituted alkyl can be obtained by alkylation of I-e with an alkyl halide (I-f) in the presence of a base (e.g., NEt 3 , Cs 2 CO 3 , etc.), in a solvent (e.g., DCM, DMF, etc.), and optionally at elevated temperature. wherein X 1 is CR 3 , and R 1 , R 2 , R 3 , Rx, n, n1, and q are as defined in Formula (I').

[0250] The general way of preparing Compounds of Formula (I') (wherein X 1 is CH and P is R 2 ) and intermediate I-d (wherein X 1 is CH and P is a protecting group) by using intermediate I-h, is outlined in General Scheme II. Coupling of I-a with zincate I-h using a catalyst (e.g., XphosPd G2) in a solvent (e.g. THF) at elevated temperature yields Compounds of Formula (I') or intermediate I-d. When P is an amine protecting group (e.g., tert-butyloxycarbonyl (Boc)) intermediate I-d is deprotected using a strong acid such as trifluoroacetic acid (TFA) or hydrochloric acid (HCl) in a solvent (e.g., tetrahydrofuran (THF), 1,2-dichloroethane or dichloromethane (DCM)) optionally at elevated temperature to provide I-e which can be further functionalized as described in General Scheme I. When P is the desired substituent then the product equates to a compound of Formula (I'). wherein X 1 is CR 3 , and R 1 , R 2 , R 3 , R x , n, n1, and q are as defined in Formula (I').

[0251] The general way of preparing Compounds of Formula (I') (wherein X 1 is CH and P is R 2 ) and intermediate I-d (wherein X 1 is CH and P is a protecting group) by using intermediate I-i, is outlined in General Scheme III. Coupling of I-a with tosylate I-i using a catalyst (e.g. NiBr 2 •DME with 4,4-di-tert-butyl-2,2'-dipyridyl (di-t-Bu-bipy), and manganese powder (Mn)), with potassium iodide (KI), and a base (e.g., 4-ethyl-pyridine) in a solvent (e.g., N,N-dimethylacetamide (DMA)) at elevated temperature yields Compounds of Formula (I') or intermediate I-d. wherein X 1 is CR 3 , and R 1 , R 2 , R 3 , R x , n, n1, and q are as defined in Formula (I').

[0252] The general way of preparing Compounds of Formula (I') (wherein X 1 is CH and P is R 2 ) and intermediate I-d (wherein X 1 is CH and P is a protecting group) by using intermediates I-j, is outlined in General Scheme IV. Coupling of I-a with bromide I-j using a catalyst (e.g. NiI 2 with 4,4-di-tert-butyl-2,2'-dipyridyl (di-t-Bu-bipy), magnesium chloride and manganese powder (Mn)), with a base (e.g., 4-ethyl-pyridine), in a solvent (e.g., N,N-dimethylacetamide (DMA)) at elevated temperature yields Compounds of Formula (I') or intermediate I-d. wherein R 1 , R 2 , R x , q, n, and n1 are as defined in Formula (I').

[0253] The general way of preparing Compounds of Formula (I') wherein X 1 is CH and ------ is a single bond and intermediate I-p using intermediates I-k, I-l, I-m, I-n, and I-o is outlined in General Scheme V. Coupling of I-k with I-l using a catalyst (e.g., NiBr 2 •DME with 4,4-di-tert-butyl-2,2'-dipyridyl (di-t-Bu-bipy) or 2-amidinopyridine), manganese powder (Mn), and potassium iodide (KI), in a solvent (e.g. N,N-dimethylacetamide (DMA)) optionally at elevated temperature yields intermediate I-m. Intermediate I-m can then be converted to the corresponding haloester I-n using thionyl chloride (SOCl 2 ) in a solvent (e.g. EtOH) and optionally at elevated temperature. Cyclization with 3-aminopiperidine-2,6-dione I-o or its HCl or CF 3 CO 2 H salt using a base (e.g., i-Pr 2 NEt) in a solvent (e.g. DMF) and optionally at elevated temperature provides compounds of Formula (I') or intermediate I-p.

[0254] A mixture of enantiomers, diastereomers, and cis / trans isomers resulting from the process described above can be separated into their single components by chiral salt technique, chromatography using normal phase, reverse phase or chiral column, depending on the nature of the separation.

[0255] Any resulting racemates of compounds of the present invention or of intermediates can be resolved into the optical antipodes by known methods, e.g., by separation of the diastereomeric salts thereof, obtained with an optically active acid or base, and liberating the optically active acidic or basic compound. In particular, a basic moiety may thus be employed to resolve the compounds of the present invention into their optical antipodes, e.g., by fractional crystallization of a salt formed with an optically active acid, e.g., tartaric acid, dibenzoyl tartaric acid, diacetyl tartaric acid, di-O,O'-p-toluoyl tartaric acid, mandelic acid, malic acid, or camphor-10-sulfonic acid. Racemic compounds of the present invention or racemic intermediates can also be resolved by chiral chromatography, e.g., high pressure liquid chromatography (HPLC) using a chiral adsorbent.

[0256] Any resulting mixtures of stereoisomers can be separated on the basis of the physicochemical differences of the constituents, into the pure or substantially pure geometric or optical isomers, diastereomers, racemates, for example, by chromatography and / or fractional crystallization.

[0257] It should be understood that in the description and formula shown above, the various groups R 1 , R 2 , R 3 , R x , n, n1, and q and other variables are as defined above, except where otherwise indicated. Furthermore, for synthetic purposes, the compounds of General Schemes I, II, III, IV, and V are merely representative with elected radicals to illustrate the general synthetic methodology of the Compounds of Formula (I') as defined herein.F. Methods of Using Compounds of Formula (I')

[0258] In another aspect, the present invention relates to a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, or a composition comprising a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of cancer.

[0259] Another aspect of the invention relates to a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, or a composition comprising a compound of Formula (I') or Formula (I), or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of an IKZF2-dependent disease or disorder.

[0260] The compounds of the present invention can be used for the treatment, of cancers including, but not limited to, liposarcoma, neuroblastoma, glioblastoma, bladder cancer, adrenocortical cancer, multiple myeloma, colorectal cancer, non-small cell lung cancer (NSCLC), triple-negative breast cancer (TNBC), nasopharyngeal cancer (NPC), microsatellite stable colorectal cancer (mssCRC), thymoma, carcinoid, gastrointestinal stromal tumor (GIST), Human Papilloma Virus-associated cervical, oropharyngeal, penis, anal, thyroid, or vaginal cancer or Epstein-Barr Virus-associated nasopharyngeal carcinoma, gastric cancer, rectal cancer, thyroid cancer, Hodgkin lymphoma or diffuse large B-cell lymphoma. the cancer is selected from prostate cancer, breast carcinoma, lymphomas, leukaemia, myeloma, bladder carcinoma, colon cancer, cutaneous melanoma, hepatocellular carcinoma, endometrial cancer, ovarian cancer, cervical cancer, lung cancer, renal cancer, glioblastoma multiform, glioma, thyroid cancer, parathyroid tumor, nasopharyngeal cancer, tongue cancer, pancreatic cancer, esophageal cancer, cholangiocarcinoma, gastric cancer, soft tissue sarcomas, rhabdomyosarcoma (RMS), synovial sarcoma, osteosarcoma, rhabdoid cancers, cancer for which the immune response is deficient, an immunogenic cancer, and Ewing's sarcoma.

[0261] One therapeutic use of the compounds or compositions of the present invention, which modulate IKZF2 and / or IKZF4 protein levels by degradation of IKZF2 and / or IKZF4, is to provide treatment to patients or subjects suffering from cancer and metastasis.

[0262] The disclosed compounds of the invention can be administered in effective amounts to treat or prevent a disorder and / or prevent the development thereof in subjects.

[0263] Compounds of the invention can be administered in therapeutically effective amounts in a combinational therapy with one or more therapeutic agents (pharmaceutical combinations) or modalities, e.g., non-drug therapies. For example, synergistic effects can occur with other anti-proliferative, anti-cancer, immunomodulatory or anti-inflammatory substances. Where the compounds of the invention are administered in conjunction with other therapies, dosages of the co-administered compounds will of course vary depending on the type of co-drug employed, on the specific drug employed, on the condition being treated and so forth.

[0264] Combination therapy includes the administration of the subject compounds in further combination with other biologically active ingredients (such as, but not limited to, a second and different antineoplastic agent or a second agent that targets Helios or another cancer target) and non-drug therapies (such as, but not limited to, surgery or radiation treatment). For instance, the compounds of the invention can be used in combination with other pharmaceutically active compounds, preferably compounds that are able to enhance the effect of the compounds of the invention. The compounds of the invention can be administered simultaneously (as a single preparation or separate preparation) or sequentially to the other drug therapy or treatment modality. In general, a combination therapy envisions administration of two or more drugs during a single cycle or course of therapy.G. Administration, Pharmaceutical Compositions, and Dosing of Compounds of Formula (I')

[0265] Administration of the disclosed compounds can be accomplished via any mode of administration for therapeutic agents. These modes include systemic or local administration such as oral, nasal, parenteral, transdermal, subcutaneous, vaginal, buccal, rectal or topical administration modes.

[0266] Depending on the intended mode of administration, the disclosed compositions can be in solid, semi-solid or liquid dosage form, such as, for example, injectables, tablets, suppositories, pills, time-release capsules, elixirs, tinctures, emulsions, syrups, powders, liquids, suspensions, or the like, sometimes in unit dosages and consistent with conventional pharmaceutical practices. Likewise, they can also be administered in intravenous (both bolus and infusion), intraperitoneal, subcutaneous or intramuscular form, and all using forms well known to those skilled in the pharmaceutical arts.

[0267] Illustrative pharmaceutical compositions are tablets and gelatin capsules comprising a compound of the invention and a pharmaceutically acceptable carrier, such as a) a diluent, e.g., purified water, triglyceride oils, such as hydrogenated or partially hydrogenated vegetable oil, or mixtures thereof, com oil, olive oil, sunflower oil, safflower oil, fish oils, such as EPA or DHA, or their esters or triglycerides or mixtures thereof, omega-3 fatty acids or derivatives thereof, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, sodium, saccharin, glucose and / or glycine; b) a lubricant, e.g., silica, talcum, stearic acid, its magnesium or calcium salt, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and / or polyethylene glycol; for tablets also; c) a binder, e.g., magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, magnesium carbonate, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, waxes, and / or polyvinylpyrrolidone, if desired; d) a disintegrant, e.g., starches, agar, methyl cellulose, bentonite, xanthan gum, algic acid or its sodium salt, or effervescent mixtures; e) absorbent, colorant, flavorant and sweetener; f) an emulsifier or dispersing agent, such as Tween 80, Labrasol, HPMC, DOSS, caproyl 909, labrafac, labrafil, peceol, transcutol, capmul MCM, capmul PG-12, captex 355, gelucire, vitamin E TGPS or other acceptable emulsifier; and / or g) an agent that enhances absorption of the compound such as cyclodextrin, hydroxypropyl-cyclodextrin, PEG400, PEG200.

[0268] Liquid, particularly injectable, compositions can, for example, be prepared by dissolution, dispersion, etc. For example, the disclosed compound is dissolved in or mixed with a pharmaceutically acceptable solvent such as, for example, water, saline, aqueous dextrose, glycerol, ethanol, and the like, to thereby form an injectable isotonic solution or suspension. Proteins such as albumin, chylomicron particles, or serum proteins can be used to solubilize the disclosed compounds.

[0269] The disclosed compounds can be also formulated as a suppository that can be prepared from fatty emulsions or suspensions; using polyalkylene glycols such as propylene glycol, as the carrier.

[0270] The disclosed compounds can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, containing cholesterol, stearylamine or phosphatidylcholines.

[0271] In some embodiments, a film of lipid components is hydrated with an aqueous solution of drug to a form lipid layer encapsulating the drug, as described in U.S. Pat. No. 5,262,564.

[0272] Disclosed compounds can also be delivered by the use of monoclonal antibodies as individual carriers to which the disclosed compounds are coupled. The disclosed compounds can also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxyethylaspanamidephenol, or polyethyleneoxidepolylysine substituted with palmitoyl residues. Furthermore, the disclosed compounds can be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and cross-linked or amphipathic block copolymers of hydrogels. In one embodiment, disclosed compounds are not covalently bound to a polymer, e.g., a polycarboxylic acid polymer, or a polyacrylate.

[0273] Parental injectable administration is generally used for subcutaneous, intramuscular or intravenous injections and infusions. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions or solid forms suitable for dissolving in liquid prior to injection.

[0274] Another aspect of the invention is directed to pharmaceutical compositions comprising a compound of Formula (I') and a pharmaceutically acceptable carrier. The pharmaceutical acceptable carrier may further include an excipient, diluent, or surfactant.

[0275] Compositions can be prepared according to conventional mixing, granulating or coating methods, respectively, and the present pharmaceutical compositions can contain from about 0.1% to about 99%, from about 5% to about 90%, or from about 1% to about 20% of the disclosed compound by weight or volume.

[0276] In one embodiment, the disclosure provides a kit comprising two or more separate pharmaceutical compositions, at least one of which contains a compound of the present invention. In one embodiment, the kit comprises means for separately retaining said compositions, such as a container, divided bottle, or divided foil packet. An example of such a kit is a blister pack, as typically used for the packaging of tablets, capsules and the like.

[0277] The kit of the disclosure may be used for administering different dosage forms, for example, oral and parenteral, for administering the separate compositions at different dosage intervals, or for titrating the separate compositions against one another. To assist compliance, the kit of the disclosure typically comprises directions for administration.

[0278] The dosage regimen utilizing the disclosed compound is selected in accordance with a variety of factors including type, species, age, weight, sex, and medical condition of the patient; the severity of the condition to be treated; the route of administration; the renal or hepatic function of the patient; and the particular disclosed compound employed. A physician or veterinarian of ordinary skill in the art can readily determine and prescribe the effective amount of the drug required to prevent, counter or arrest the progress of the condition.

[0279] Effective dosage amounts of the disclosed compounds, when used for the indicated effects, range from about 0.5 mg to about 5000 mg of the disclosed compound as needed to treat the condition. Compositions for in vivo or in vitro use can contain about 0.5, 5, 20, 50, 75, 100, 150, 250, 500, 750, 1000, 1250, 2500, 3500, or 5000 mg of the disclosed compound, or, in a range of from one amount to another amount in the list of doses. In one embodiment, the compositions are in the form of a tablet that can be scored.EXAMPLES

[0280] The invention is further illustrated by the following examples and synthesis schemes, which are not to be construed as limiting this invention in scope to the specific procedures herein described. It is to be understood that the examples are provided to illustrate certain embodiments.

[0281] Compounds of the present invention may be prepared by methods known in the art of organic synthesis. In all of the methods it is understood that protecting groups for sensitive or reactive groups may be employed where necessary in accordance with general principles of chemistry. Protecting groups are manipulated according to standard methods of organic synthesis (T.W. Green and P.G.M. Wuts (1999) Protective Groups in Organic Synthesis, 3rd edition, John Wiley & Sons). These groups are removed at a convenient stage of the compound synthesis using methods that are readily apparent to those skilled in the art.Analytical Methods, Materials, and Instrumentation

[0282] Unless otherwise noted, reagents and solvents were used as received from commercial suppliers. Proton nuclear magnetic resonance (NMR) spectra were obtained on either Bruker Avance spectrometer or Varian Oxford 400 MHz spectrometer unless otherwise noted. Spectra are given in ppm (δ) and coupling constants, J, are reported in Hertz. Tetramethylsilane (TMS) was used as an internal standard. Chemical shifts are reported in ppm relative to dimethyl sulfoxide (δ 2.50), methanol (δ 3.31), chloroform (δ 7.26) or other solvent as indicated in NMR spectral data. A small amount of the dry sample (2-5 mg) is dissolved in an appropriate deuterated solvent (1 mL). The chemical names were generated using ChemBioDraw Ultra v12 from Cambridge Soft.

[0283] Mass spectra (ESI-MS) were collected using a Waters System (Acquity UPLC and a Micromass ZQ mass spectrometer) or Agilent-1260 Infinity (6120 Quadrupole); all masses reported are the m / z of the protonated parent ions unless recorded otherwise. The sample was dissolved in a suitable solvent such as MeCN, DMSO, or MeOH and was injected directly into the column using an automated sample handler. The analysis is performed on Waters Acquity UPLC system (Column: Waters Acquity UPLC BEH C18 1.7µm, 2.1 x 30mm; Flow rate: 1 mL / min; 55°C (column temperature); Solvent A: 0.05% formic acid in water, Solvent B: 0.04% formic acid in MeOH; gradient 95% Solvent A from 0 to 0.10 min; 95% Solvent A to 20% Solvent A from 0.10 to 0.50 min; 20% Solvent A to 5% Solvent A from 0.50 to 0.60 min; hold at 5% Solvent A from 0.6 min to 0.8 min; 5% Solvent A to 95% Solvent A from 0.80 to 0.90 min; and hold 95% Solvent A from 0.90 to 1.15 min.Abbreviations used in the following examples and elsewhere herein are:

[0284] AIBNazobisisobutyronitrile Bnbenzyl brbroad Bu 4 NItetrabutylammonium iodide ddoublet dddoublet of doublets ddddoublet of doublet of doublets ddqdoublet of doublet of quartets ddtdoublet of doublet of triplets dqdoublet of quartets dtdoublet of triplets dtddoublet of triplet of doublets CCl 4 carbon tetrachloride Cs 2 CO 3 cesium carbonate Cu(OAc) 2 copper (II) acetate DCMdichloromethane di-tBu-bipy4,4'-di-tert-butyl-2,2'-dipyridyl DIBAL-HDiisobutylaluminium hydride DMAN,N-dimethylacetamide DMAP4-dimethylaminopyridine DME1,2-Dimethoxyethane DMFN,N-dimethylformamide DMPDess-Martin periodinane or 1,1,1-Tris(acetyloxy)-1,1-dihydro-1,2-benziodoxol-3-(1H)-one DMSOdimethylsulfoxide EC 50 half maximal effective concentration Et 2 Odiethyl ether EtOAcethyl acetate 4-Et-Py4-ethylpyridine HATU1-[Bis(dimethylamino)methylene]-1H-1,2,3 -triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate HClhydrogen chloride heptheptet HPLChigh performance liquid chromatography h or hrhour HRMShigh resolution mass spectrometry ggram IC 50 half maximal inhibitory concentration K 2 CO 3 potassium carbonate KIpotassium iodide K 3 PO 4 tripotassium phosphate KOAcpotassium acetate LiAlH 4 Lithium aluminum hydride LCMSliquid chromatography mass spectrometry LiHMDSLithium bis(trimethylsilyl)amide mmultiplet MeCNacetonitrile MeOHmethanol mgmilligram MgCl 2 magnesium chloride MHzmegahertz minminutes mLmilliliter mmolmillimole Mmolar MSmass spectrometry NaBH(OAc) 3 sodium triacetoxyborohydride NaHCO 3 sodium bicarbonate Na 2 SO 4 sodium sulfate NBSN-bromosuccinimide NEt 3 triethylamine NH 4 OAcammonium acetate NH 4 OHammonium hydroxide NiBr 2 (DME)nickel (II) bromide ethylene glycol dimethyl ether complex NiBr 2 (glyme)nickel (II) bromide ethylene glycol dimethyl ether complex NiI 2 nickel (II) iodide NMRNuclear magnetic resonance PCCPyridinium chlorochromate PdCl 2 (dppf) 2 [1,1'-Bis(diphenylphosphino)ferrocene]palladium(II) dichloride PdCl 2 (dppf)•DCM[1,1'-Bis(diphenylphosphino)ferrocene]dichloropalladium(II), complex with dichloromethane\Pd(Ph 3 P) 4 tetrakis(triphenylphosphine)palladium(0) PtO 2 platinum (IV) oxide qquartet qdquartet of doublets quintquintet quintdquintet of doublets rtroom temperature Rtretention time ssinglet SFCsupercritical fluid chromatography ttriplet TEAtriethylamine tdtriplet of doublets tddtriplet of doublet of doublets THFtetrahydrofuran Ti(Oi-Pr) 4 titanium isopropoxide TfOHtriflic acid Tstosyl TsCl4-toluenesulfonyl chloride tttriplet of triplets ttdtriplet of triplet of doublets TLCthin-layer chromatography UPLCultra-Performance Liquid Chromatography Xphos Pd G2chloro(2-dicyclohexylphosphino-2',4',6'-triisopropyl-1,1-biphenyl)[2-(2'-amino-1,1'-biphenyl)]palladium(II) µWmicrowave Reference Example 1: 3-(1-oxo-5-(piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (1-155)

[0285]

[0286] Intermediate 1a was prepared as reported in U.S. Patent Application US 2009 / 0142297.

[0287] To a stirred solution of methyl 4-bromo-2-(bromomethyl)benzoate (1a , 15 g, 48.7 mmol) in DMF (150 mL) was added 3-aminopiperidine-2,6-dione-HCl (1b , 6.9 g, 53.6 mmol) and K 2 CO 3 (20.2 g, 146.1 mmol). The resulting mixture was heated at 70 °C for 16 h after which time the reaction mixture was cooled to rt and then concentrated to dryness. To the resulting residue, water was added and the mixture stirred at rt for 30 min. The resultant solid was filtered and washed with ether and ethyl acetate. The solid was dried under vacuum filtration to afford 1c (10.6 g, 32.9 mmol, 67% yield). MS [M+H] +< = 323.0. 1< H NMR (400 MHz, DMSO-d 6 ) δ 10.99 (s, 1H), 7.91-7.88 (m, 1H), 7.72 (dd, J = 8.1, 1.6 Hz, 1H), 7.67 (d, J = 8.0 Hz, 1H), 5.11 (dd, J = 13.3, 5.1 Hz, 1H), 4.47 (d, J = 17.7 Hz, 1H), 4.34 (d, J = 17.7 Hz, 1H), 2.98-2.83 (m, 1H), 2.65-2.55 (m, 1H), 2.45-2.29 (m, 1H), 2.01 (dtd, J = 12.7, 5.3, 2.3 Hz, 1H).Step 2. tert-Butyl-4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)-3,6-dihydropyridine-1(2H)-carboxylate (1e)

[0288] A solution of Ic (1.8 g, 5.6 mmol) in DMF (10 mL) in a sealed tube was purged with argon for 5 min prior to addition of 3,6-dihydro-2H-pyridine-1-tert-butoxycarbonyl-4-boronic acid pinacol ester (1d , 2.2g, 7.2 mmol), K 3 PO 4 (1.42g, 6.7 mmol) and Pd(dppf)Cl 2 •DCM (227mg, 0.28 mmol). The reaction mixture was again purged with argon for 5 min and then heated at 90 °C for 16 h. After this time the reaction mixture was cooled to rt and then concentrated under reduced pressure. Water was added to the residue which was then extracted with EtOAc. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered, and then concentrated under a reduced pressure. The crude compound was purified by silica gel chromatography, eluting with 70-80% of EtOAc in hexanes, to afford 1e as a light brown solid (1.0 g, 2.4 mmol, 42% yield). MS [M+H]+ = 426.3.Step 3. tert-Butyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidine-1-carboxylate (1f):

[0289] To a stirred solution of 1e (1.0 g, 2.35 mmol) in DMF (20 mL) was added 10% Pd / C (150 mg) and the mixture was stirred under a hydrogen atmosphere (balloon) at rt for 6 h. The reaction mixture was then filtered through a bed of Celite ®< filter aid. The filtrate was concentrated under reduced pressure affording If as an off-white solid (0.85 g, 1.97 mmol, 84% yield). MS [M-tBu] +< = 372.3. 1< H NMR (400 MHz, CDCl 3 ) δ 8.40 (s, 1H), 7.81 (d, J = 7.9 Hz, 1H), 7.32 (d, J = 8.0 Hz, 1H), 7.29 (s, 1H), 5.22 (dd, J = 13.3, 5.1 Hz, 1H), 4.46 (d, J = 16.0 Hz, 1H), 4.31 (d, J = 16.1 Hz, 1H), 4.27 (d, J = 16.2 Hz, 2H), 2.97-2.67 (m, 5H), 2.41-2.26 (m, 1H), 2.23-2.13 (m, 1H), 1.83 (d, J = 12.6 Hz, 2H), 1.71-1.55 (m, 2H), 1.48 (s, 9H).Step 4. 3-(1-oxo-5-(piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione hydrochloride (1-155):

[0290] To a stirred solution of If (0.85 g, 2.0 mmol) in dioxane (10 mL) was added 4N HCl in dioxane (5.0 mL). The reaction mixture was then stirred at rt for 2 h. The reaction mass was concentrated under reduced pressure to afford the HCl salt of desired compound 1-155 as an off-white solid (0.65 g, 1.8 mmol, 90% yield, hydrochloride salt). MS [M+H] +< = 328.3. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.99 (s, 1H), 9.28 (s, 1H), 7.70 (d, J = 7.8 Hz, 1H), 7.46 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.74 (s, 1H), 5.11 (dd, J = 13.3, 5.2 Hz, 1H), 4.46 (d, J = 17.3 Hz, 1H), 4.32 (d, J = 17.3 Hz, 1H), 3.36 (d, J = 11.5 Hz, 2H), 3.10-2.86 (m, 4H), 2.61 (d, J = 14.8 Hz, 1H), 2.39 (qd, J = 13.2, 4.3 Hz, 1H), 2.14-1.79 (m, 5H).Conversion of 1c to 1f was also achieved in a single step via Negishi coupling using the following procedure:

[0291]

[0292] 1-(tert-butoxycarbonyl)piperidin-4-yl)zinc(II) iodide (1g ) was prepared as reported in Corley, E. G., et al., J. Org. Chem. 2004, 69, 5120.

[0293] A mixture of 1c (41 mg, 0.125 mmol) and XPhos Pd cycle G2 (15 mg, 0.019 mmol) in THF (1.5 mL) was purged with nitrogen prior to addition of (1-(tert-butoxycarbonyl)piperidin-4-yl)zinc(II) iodide (1g, 0.142 mg, 0.376 mmol) in THF (0.7 mL). The resulting mixture was heated to 50 °C for 1 h after which time the reaction was cooled to rt, quenched with brine, and extracted with EtOAc. The organic layer was passed through a phase separator and concentrated. The crude material was purified by silica gel chromatography (eluting with 0-100% EtOAc in heptane) to afford If as a white solid (30 mg, 0.070 mmol, 56% yield).Alternatively, conversion of 1c to 1f was also achieved in a single step via the following reductive cross-coupling procedure:

[0294]

[0295] To a mixture of 1c (934 mg, 2.89 mmol), tert-butyl 4-bromopiperidine-1-carboxylate (1h, 1530 mg, 5.80 mmol), NiI 2 (90 mg, 0.289 mmol), di-t-Bu-bipy (78 mg, 0.289 mol), MgCl 2 (275 mg, 2.89 mmol), and manganese powder (317 mg, 5.78 mmol) in DMA (5 mL) was added 4-ethylpyridine (0.33 mL, 2.89 mmol) and the reaction mixture was stirred vigorously for 18 h at 60 °C. The reaction mixture was filtered through a short pad of Celite ®< filter aid and eluted with EtOAc. The obtained solution was then concentrated by azeotroping with heptane. The crude product was purified via chromatography on silica gel eluting with MeOH in DCM to afford If (285 mg, 0.653 mmol, 23% yield) as a white solid.Conversion of 1c to 1f was also achieved in a single step via an alternative reductive cross-coupling procedure:

[0296]

[0297] To crude 1c (84% pure, 34 mg, 0.088 mmol), tert-butyl 4-(tosyloxy)piperidine-1-carboxylate (1i, 38 mg, 0.11 mmol), NiBr 2 · DME (2.7 mg, 8.8 µmol), di-t-Bu-bipy (2.4 mg, 8.8 µmol), KI (15 mg, 0.09 mmol) and manganese powder (10 mg, 0.18 mmol) in DMA (0.50 mL) was added 4-ethylpyridine (10 µL, 0.088 mmol) and the reaction mixture was stirred vigorously at 75 °C for 5 h. The reaction mixture was filtered through a short pad of Celite ®< filter aid and eluted with MeCN. The obtained solution was concentrated by azeotroping with heptane. The crude product was purified via chromatography on silica gel eluting with MeOH in DCM to afford If (21.7 mg, 0.051 mmol, 57% yield) as a white solid.In a similar fashion, intermediate 1f could be obtained from intermediate 29d, (the route for synthesis of 29d is outlined in Example 29):

[0298]

[0299] To 29d (48 mg, 0.13 mmol), tert-butyl 4-(tosyloxy)piperidine-1-carboxylate (1i, 55 mg, 0.16 mmol), NiBr 2 ·DME (4.0 mg, 0.013 mmol), di-t-Bu-bipy (3.5 mg, 0.013 mmol), KI (22 mg, 0.13 mmol) and manganese powder (14 mg, 0.26 mmol) in DMA (0.67 mL) was added 4-ethylpyridine (0.015 mL, 0.14 mmol) and the reaction mixture was stirred vigorously at 80 °C for 5 h. The reaction mixture was filtered through a short pad of Celite ®< filter aid and eluted with MeCN. The obtained solution was concentrated by azeotroping with heptane. The crude product was purified via chromatography on silica gel eluting with MeOH in DCM to afford If (33.3 mg, 0.078 mmol, 60% yield) as a white solid.Reference Example 2: 3-(1-oxo-5-(2,2,6,6-tetramethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-171)

[0300] Step 1: 3-(1-oxo-5-(2,2,6,6-tetramethyl-1,2,3,6-tetrahydropyridin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (2b)

[0301] A stirred solution of 1c (150 mg, 0.46 mmol) in DMF (5 mL) in a sealed tube was purged with argon for 5 min prior to the addition of 2,2,6,6-tetramethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)piperidine (2a, 185 mg, 0.69 mmol), Cs 2 CO 3 (300 mg, 0.92 mmol), and Pd(dppf)Cl 2 •DCM (19 mg, 0.02 mmol) and the resulting mixture was again purged with argon for 5 min. The reaction mixture was then heated at 90°C for 5 h after which time the reaction mixture was cooled to rt, water was added, and was extracted with EtOAc. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered, and then concentrated under reduced pressure. The crude material was purified by silica gel chromatography (eluting with 15% MeOH / DCM) to afford 2b as a brown solid (35 mg, 0.092 mmol, 20% yield). MS [M+H] +< = 382.3.Step 2. 3-(1-oxo-5-(2,2,6,6-tetramethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-171)

[0302] To a stirred solution of 3-(1-oxo-5-(2,2,6,6-tetramemyl-1,2,3,6-tetrahydropyridin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (2b, 25 mg, 0.07 mmol) in DMF (2 mL) was added Pd / C (5 mg). The resulting mixture was stirred under a hydrogen atmosphere (balloon) at rt for 5 h. The reaction mixture was then filtered through a Celite ®< filter aid pad and the filtrate was concentrated to dryness. The crude material was purified by reverse phase HPLC (MeCN / H 2 O with 0.05% formic acid). The fractions containing the desired product were collected and concentrated to dryness affording I-171 as an off-white solid (11 mg, 0.03 mmol, 44% yield). MS [M+H] +< = 384.4. 1< H NMR (600 MHz, DMSO-d 6 ): δ 11.0 (s, 1H), 8.33-8.32 (m, 1H), 7.68 (d, J = 8.0 Hz, 1H), 7.5 (s, 1H), 7.43 (d, J = 8.4 Hz, 1H), 5.12 (dd, J = 13.2, 4.8 Hz, 1H), 4.36 (d, J = 17.4 Hz, 1H), 4.31 (d, J = 17.4 Hz, 1H), 2.95-2.90 (m, 1H), 2.43-2.39 (m, 2H), 2.00-1.99 (m, 2 H), 1.54-1.50 (m, 2H), 1.52-1.50 (m, 2H), 1.26 (s, 6H), 1.23 (s, 6H).Example 3: Diastereomers of 3-(5-(1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-190) and (I-273)

[0303] Step 1: 3-(1-oxo-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)isoindolin-2-yl)piperidine-2,6-dione (3b)

[0304] To a stirred solution of 1c (3.0 g, 9.28 mmol) in DMF (20 mL) in a sealed tube was added bis(pinacolato)diboron (3a, 2.6 g, 10.2 mmol), KOAc (2.37g, 27.9 mmol), and PdCl 2 (dppf) 2 (0.22 g, 0.28 mmol). The reaction mixture was purged with argon for 5 min, sealed, and then heated at 100 °C for 16 h. Water was added to the reaction mixture and stirred at rt for 15 min. The solid was precipitated, filtered, and dried under vacuum to afford 3b as brown solid (2.3 g, 6.2 mmol, 66% yield). MS [M+H] +< = 371.0. Step 2: tert-butyl 5-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)-2,3,4,7-tetrahydro-1H-azepine-1-carboxylate (3d) tert-Butyl 5-(((trifluoromethyl)sulfonyl)oxy)-2,3,4,7-tetrahydro-1H-azepine-1-carboxylate 3c was prepared as reported in PCT Application Publication No. 2007 / 111904.

[0305] To a stirred solution of 3b (1.0 g, 2.70 mmol) in DMF (10.0 mL) in a sealed tube was added tert-butyl 5-(((trifluoromethyl)sulfonyl)oxy)-2,3,4,7-tetrahydro-1H-azepine-1-carboxylate (3c, 1.19 g, 3.24 mmol), Pd(PPh 3 ) 4 (0.16 g, 0.13 mmol), and Na 2 CO 3 (0.85 g, 8.10 mmol). The mixture was purged with argon for 5 min and then sealed and heated at 100 °C for 3 h. After this time, the reaction was cooled and water added prior to extraction with EtOAc. The organic layer was dried over Na 2 SO 4 , filtered, concentrated under reduced pressure, and purified by silica gel chromatography (eluting with 60-70% EtOAc / hexanes) to afford 3d as a brown solid (350 mg, 0.796 mmol, 29% yield). MS [M+H] +< = 440.0.Step 3: tert-butyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)azepane-1-carboxylate (3e).

[0306] To a stirred solution of 3d (0.35 g, 0.80 mmol) in THF (10 mL) was added PtO 2 (100 mg). The mixture was stirred under hydrogen balloon for 5 h. The reaction mixture was then filtered on a bed of Celite ®< filter aid and then concentrated under reduced pressure. The resulting residue was purified by silica gel chromatography (eluting with 40-50% EtOAc / hexane) to afford 3e as a white solid consisting of a mixture of diastereomers (0.31 g, 0.70 mmol, 88% yield). MS [M+H] +< = 442.0.Step 4a: chiral separation of tert-butyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)azepane-1-carboxylate (3e)

[0307] Chiral separation of 3e (350 mg) was performed using a Kinetex (150 mm X 21 mm), 5.0 µ column, with eluent consisting of mobile phase A = 0.05% TFA in water; mobile phase B = acetonitrile and a flow rate of 20 mL / min at 25 °C with 20-70% mobile phase B: mobile phase A over 20 min. Under these conditions two compounds were isolated 3e (peak 1) Rt = 11.64 min and 3e (peak 2) Rt = 17.41 min). The fractions corresponding to peak 1 and peak 2 were collected and concentrated under reduced pressure then neutralized with aqueous saturated NaHCO 3 solution prior to extraction with DCM. The organic layers were combined, dried over Na 2 SO 4 , filtered, and concentrated to dryness affording peak 1 (50 mg) and peak 2 (45 mg) as white solids. MS [M+H] +< = 442.0.Step 4b: 3-(5-(azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-166 & 1-167)

[0308] To a stirred solution of 3e (peak 1) (50 mg, 0.113 mmol) in dioxane (2.0 mL) at 0 °C was added 4M HCl in dioxane (0.5 mL). The reaction was then allowed to stir and warm up to rt over 2 h. The reaction mixture was then concentrated under reduced pressure to afford diastereomer A as a white solid (40 mg, 0.106 mmol, 94%, hydrochloride salt). MS [M+H] +< = 342.3. 1< H NMR (CD 3 OD, 300 MHz): δ 7.74 (d, J = 8.1 Hz, 1H), 7.49 (1H, s), 7.43 (d, J = 8.4 Hz, 1H), 5.14 (dd, J = 13.5, 5.1 Hz, 1H), 4.48-4.46 (m, 2H), 3.74-3.71(m, 1H), 3.68-3.63 (m, 2H), 3.59-3.55 (m, 1H), 3.44-3.37 (m, 2H), 3.02 (m, 1H), 2.90-2.78 (m, 2H), 2.51-2.47 (m, 1H), 2.16-2.08 (m, 5H), 2.00-1.80 (m, 2H).

[0309] To a stirred solution of 3e (peak 2) (40 mg, 0.091 mmol) in dioxane (2.0 mL) at 0 °C was added 4M HCl in dioxane (0.5 mL). The reaction was then allowed to stir and warm up to rt over 2 h. The reaction mixture was then concentrated under reduced pressure to afford diastereomer B as a white solid (30 mg, 0.079 mmol, 87% yield, hydrochloride salt). MS [M+H] +< = 342.4. 1< H NMR (CD 3 OD, 300 MHz): δ 7.74 (d, J= 7.5 Hz, 1H), 7.49 (s, 1H), 7.42 (d, J= 7.8 Hz, 1H), 5.15 (dd, J= 13.5, 5.1 Hz, 1H), 4.47-4.45 (d, 2H), 3.74-3.71 (m, 1H), 3.67-3.62 (m, 3H), 3.58-3.55 (m, 1H), 3.43-3.38 (m, 2H), 3.02 (m, 1H), 2.90-2.78 (m, 2H), 2.51-2.48 (m, 1H), 2.17-2.08 (m, 5H), 2.09-1.87 (m, 1H).Step 5. Diastereomers of 3-(5-(1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-190 and I-273)

[0310] Compound I-190 was prepared from I-166 (80 mg, 0.21 mmol) and benzaldehyde (27 mg, 0.25 mmol) via reductive amination as described for Example 8. After complete consumption of starting materials, the crude reaction mixture was concentrated under reduced pressure and sat. aq. NaHCO 3 was added. The resulting mixture was extracted with DCM and the organic layer was dried over Na 2 SO 4 , filtered, and concentrated to dryness. The resulting solid was washed with ether (5 mL) and EtOAc (0.1 mL) affording 1-190 as an off-white solid (55 mg, 0.13 mmol, 60% yield). Absolute stereochemistry is not known and was arbitrarily assigned. MS [M+H]+ = 439.1. 1< H NMR (CD 3 OD, 600 MHz) : δ 7.69 (d, J = 5.2 Hz, 1H), 7.44 (s, 1H), 7.39-7.36 (m, 3H), 7.32-7.30 (m, 2H), 7.26-7.24 (m, 1H), 5.12 (dd, J= 8.8, 3.2 Hz, 1H), 4.47 (d, J = 11.6 Hz, 1H), 4.41 (d, J = 11.2 Hz, 1H), 3.71 (2H, s), 2.98-2.97 (m, 1H), 2.92-2.86 (m, 2H), 2.80-2.74 (m, 4H), 2.47-2.45 (m, 1H), 2.16-2.14 (m, 1H), 1.94-1.84 (m, 1H), 1.80 (m, 1H).

[0311] Compound I-273 was prepared from I-167 (80 mg, 0.21 mmol) and benzaldehyde (27 mg, 0.25 mmol) in a similar manner as describe above for I-190. I-273 was isolated as an off-white solid (55 mg, 0.13 mmol, 60% yield). Absolute stereochemistry is not known and was arbitrarily assigned. MS [M+H] +< = 439.1. 1< H NMR (DMSO-d 6 , 400 MHz): δ 11.0 (1H, s), 7.62 (d, J = 5.2 Hz, 1H), 7.46 (s, 1H), 7.38-7.32 (m, 5H), 7.24-7.23 (m, 1H), 5.09 (dd, J = 8.8, 3.6 Hz, 1H), 4.31 (d,J = 11.6 Hz, 1H), 4.28 (d, J = 11.6 Hz, 1H), 3.65 (d, J = 9.1 Hz, 1H), 3.63 (d, J = 9.2 Hz, 1H), 2.96-2.88 (m, 2H), 2.76-2.69 (m, 1H), 2.67-2.62 (m, 3H), 2.61- 2.50 (m, 2H), 2.46-2.36 (m, 3H), 1.99 (m, 2H), 1.82 (m, 2H).Example 4: 3-(5-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-163)

[0312]

[0313] A stirred solution of 1c (50 mg, 0.22 mmol) in DMF (3.0 mL) in a sealed tube was purged with argon for 5 min prior to the addition of 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,2,3,6-tetrahydropyridine (4a, 55 mg, 0.24 mmol), K 3 PO 4 (94 mg, 0.44 mmol), and Pd(dppf)Cl 2 •DCM (9 mg, 0.011 mmol). The resulting mixture was then purged with argon for 5 min and then heated to 90°C for 16 h. The reaction mixture was cooled to rt and then concentrated under reduced pressure. The crude material was purified by preparative TLC eluting with 10% MeOH / DCM to afford I-163 as an off white solid (9.0 mg, 0.03 mmol, 12% yield). MS [M+H] +< = 340.3. 1< H NMR (DMSO-d 6 , 400 MHz): δ 7.68 (d, J = 8.0 Hz, 1H), 7.63 (s, 1H), 7.58 (d, J = 9.2 Hz, 1H), 6.28 (brs, 1H), 5.00 (dd, J = 13.2 Hz, 5.2 Hz, 1H), 4.45 (d, J = 17.6 Hz, 1H), 4.31 (d, J = 17.6 Hz, 1H), 3.07 (brs, 2H), 2.68-2.60 (m, 4H), 2.35-2.32 (m, 2H), 2.27 (s, 3H) 2.06-1.99 (m, 1H).Example 5: methyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate (I169

[0314]

[0315] To a stirred solution of I-155 (100 mg, 0.27 mmol) in DMF (2 mL) was added NEt 3 (0.095 mL, 0.69 mmol) at 0 °C and the resulting solution was stirred for 10 min. Methyl 2-bromoacetate (5a, 50 mg, 0.33 mmol) was then added and the resulting mixture was allowed to stir at rt for 4 h. The reaction was quenched with water and extracted with EtOAc (2 x 10 mL) and the combined organic layers were washed with brine, dried over Na 2 SO 4 , and concentrated under reduced pressure to afford I-169 as a white solid (39 mg, 0.097 mmol, 36% yield). MS [M+H] +< = 400.3. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.99 (s, 1H), 7.64 (d, J = 8.1 Hz, 1H), 7.50 (s, 1H), 7.41 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.6, 5.2 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.62 (s, 3H), 3.27 (s, 2H) 2.95-2.87 (m, 3H), 2.67-2.57 (m, 2H), 2.49-2.36 (m, 1H), 2.33-2.27 (m, 2H), 2.00-1.97 (m, 1H), 1.74-1.68 (m, 4H).Example 6: 3-(1-oxo-5-(1-phenylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-170)

[0316]

[0317] To 3-(1-oxo-5-(piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione I-155 (50 mg, 0.14 mmol) in DCM (0.5 mL) at rt was added pyridine (0.63 mL, 0.46 mmol). After stirring for 10 min, phenyl boronic acid (6a, 22 mg, 0.18 mmol) and copper acetate (13 mg, 0.076 mmol) were added and the reaction mixture was stirred at rt for 24 h. Water was added and the resulting mixture was extracted with EtOAc. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure. The resulting residue was purified by reverse phase HPLC (MeCN / H 2 O). The fractions containing the desired product were concentrated to dryness affording the title compound I-170 as an off-white solid (3 mg, 5% yield). MS [M+H] +< = 404.5. 1< H NMR (CDCl 3 , 600 MHz): δ 7.90 (s, 1H), 7.83 (d, J = 7.8 Hz, 1H), 7.39 (d, J = 8.4 Hz, 1H), 7.36 (s, 1H), 7.30-7.25 (m, 2H), 7.00 (d, J = 7.8 Hz, 2H), 6.88-6.86 (m, 1H), 5.25-5.20 (m, 1H), 4.48 (d, J = 15.6 Hz, 1H), 4.33 (d, J = 15.6 Hz, 1H), 3.84-3.82 (m, 2H), 2.93-2.91 (m, 1H), 2.86-2.75 (m, 4H), 2.40-2.35 (m, 1H), 2.25-2.18 (m, 1H), 1.96-1.92 (m, 4H).Example 7: 3-(5-(1-(cyclohexylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-11)

[0318]

[0319] To a stirred solution of I-155 (20 mg, 0.055 mmol) and cyclohexanecarbaldehyde 7a (0.02 mL, 0.17 mmol) in DCM (0.6 mL) and DMF (0.6 mL) was added sodium triacetoxyborohydride (35 mg, 0.17 mmol) in one portion and the reaction mixture was stirred vigorously overnight at rt. The reaction mixture was concentrated under reduced pressure and the crude product diluted with aqueous formic acid (0.1 M in H 2 O) and MeCN. The resulting solution was directly purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The pure fractions containing the desired product were combined and concentrated to afford the formate salt ofI-11 (15.3 mg, 0.033 mmol, 59% yield, formate salt) as a white solid. MS [M+H] +< = 424.6. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.98 (s, 1H), 8.23 (s, 1H), 7.64 (d, J = 7.9 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J = 7.8 Hz, 1H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.1 Hz, 1H), 3.00-2.82 (m, 3H), 2.69-2.54 (m, 2H), 2.39 (qd, J = 13.4, 4.6 Hz, 1H), 2.12 (d, J = 7.2 Hz, 2H), 2.04-1.91 (m, 3H), 1.83-1.57 (m, 9H), 1.55-1.43 (m, 1H), 1.30-1.06 (m, 3H), 0.84 (q, J = 13.2 Hz, 2H). 13< C NMR (100 MHz, DMSO-d 6 ): δ 172.91, 171.11, 168.04, 150.71, 142.47, 129.74, 126.92, 122.92, 121.71, 65.18, 54.21, 51.53, 47.10, 42.35, 34.62, 33.12, 31.37, 31.23, 26.42, 25.60, 22.52.Example 8: 3-(5-(1-benzylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-57)

[0320]

[0321] Example 8 was prepared by two different methods:Method 1- via a reductive amination procedure:

[0322] To a stirred solution of I-155 (450 mg, 1.2 mmol) in a mixture of DMF (5 mL), DCM (5 mL), and benzaldehyde (157 mg, 1.5 mmol) was added NaBH(OAc) 3 (0.78 g, 3.7 mmol) in a single portion. The reaction mixture was stirred at rt for 16 h. The reaction mass was then concentrated under reduced pressure, neutralized with NaHCO 3 solution, and extracted in DCM. The organic layer was then dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure affording a light brown solid. The solid was then purified by column chromatography, eluting with 0-10% NEt 3 / EtOAc, affording I-57 as a white solid (210 mg, 0.50 mmol, 42% yield). MS [M+H] +< = 418.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.98 (s, 1H), 7.64 (d, J = 7.9 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J = 7.9 Hz, 1H), 7.33 (d, J = 4.4 Hz, 4H), 7.29-7.23 (m, 1H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.51 (s, 2H), 2.99-2.84 (m, 3H), 2.71-2.56 (m, 2H), 2.46-2.31 (m, 1H), 2.16-1.94 (m, 3H), 1.84-1.64 (m, 4H).Method 2- via an alkylation reaction:

[0323] To a stirred solution of I-155 (80 mg, 0.22 mmol) in DMF (0.8 mL) was added NEt 3 (0.09 mL, 0.62 mmol) and the resulting mixture was stirred for 15 min. Benzyl bromide (0.03 mL, 0.27 mmol) was then added and the reaction mixture was stirred at rt for 2 h. The reaction mixture was then concentrated to dryness and the resulting residue was washed with Et 2 O then decanted. The remaining residue was dried under high vacuum to afford I-57 as an off-white solid (50 mg, 0.12 mmol, 54% yield).Preparation of I-57 was also achieved in a single step from intermediate 29d using the following procedure, (preparation of 29d is outlined in Example 29):

[0324]

[0325] To a mixture of 29d (50 mg, 0.135 mmol), tert-butyl 1-benzylpiperidin-4-yl 4-methylbenzenesulfonate (8c, 65 mg, 0.19 mmol), NiBr 2 •DME (4.2 mg, 0.014 mmol), di-t-Bu-bipy (3.6 mg, 0.014 mmol), KI (22.4 mg, 0.135 mmol), and manganese powder (15 mg, 0.27 mmol) in DMA (0.67 mL) was added 4-ethylpyridine (15 µL, 0.14 mmol) and the reaction mixture was stirred vigorously at 80 °C for 4.5 h. The reaction mixture was then filtered through a short pad of Celite ®< filter aid and eluted with DCM. The obtained solution was concentrated by azeotroping with heptane. The crude product was purified via chromatography on silica gel eluting with NEt 3 (0-10%) in EtOAc to afford I-57 (24.5 mg, 0.059 mmol, 43.4% yield) as a white solid.

[0326] The following compounds in Table 1 were prepared from intermediate I-155 and corresponding aldehyde according to a reductive amination procedure described in Example 8 (Method 1): Table 1: Cmpd No. Compound Name MS [M+1] I-119 3-(5-(1-((1H-pyrrol-3-yl)methyl)piperidin-4-yl)-1-oxoisomdolm-2-yl)piperidine-2,6-dione407.2I-127 3-(5-(1-([1,2,4]triazolo[1,5-a]pyridin-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione459.2I-137 3-(5-(1-((1H-indazol-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione458.2I-154 3-(1-oxo-5-(1-((4-phenyl-1H-imidazol-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione484.2I-132 3-(5-(1-(imidazo[1,2-a]pyrimidin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione459.2I-141 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4]thiazin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione491.2I-136 3-(5-(1-((1H-indol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione457.2I-116 3-(5-(1-((1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione408.2I-139 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1- 11 yl)methyl)benzamide461.2I-126 3-(5-(1-(imidazo[1,2-a]pyrazin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione459.2I-131 3-(1-oxo-5-(1-(pyrazolo[1,5-a]pyrimidin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione459.2I-259 3-(1-oxo-5-(1-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione474.2I-115 3-(5-(1-((1-methyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione422.2I-121 3-(5-(1-((1-ethyl- 1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione436.2I-152 3-(1-oxo-5-(1-((2-phenyl-1H-imidazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione484.2I-129 3-(5-(1-((1,4-dimethyl-1H-imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione436.2I-143 3-(5-(1-((2-(tert-butyl)thiazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione481.2I-125 3-(5-(1-((6-methylimidazo[2,1-b]thiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione478.2I-151 3-(1-oxo-5-(1-((3-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione485.2I-147 3 -(5 -(1-((2-morpholinopyrimidin-5 -yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione505.3I-86 N-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetamide475.2I-148 3-(1-oxo-5-(1-((3-phenyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione484.2I-149 3-(5-(1-((6-methyl-1H-indol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione471.2I-120 3-(5-(1-((1H-imidazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione408.2I-146 3-(5-(1-((5-cyclopropyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione448.2I-135 3-(1-oxo-5-(1-((4,5,6,7-tetrahydropyrazolo[1,5-a]pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione462.2I-140 3-(5-(1-((1H-pyrrolo[2,3-b]pyridin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione458.2I-122 3-(5-(1-((2-aminopyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione435.2I-130 3-(5-(1-(benzo[d]thiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione475.2I-124 3-(5-(1-((5-amino-1-methyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione437.2I-123 3-(5-(1-((6-aminopyridin-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione434.2I-142 3-(1-oxo-5-(1-((2-(pyrrolidin-1-yl)pyrimidin-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione489.3I-117 3-(5-(1-((1-methyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione422.2I-201 3-(1-oxo-5-(1-((3-(pyridin-2-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione485.2I-145 3-(5-(1-((2-cyclohexylthiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione507.2I-300 3-(5-(1-((3-((3S,5S)-adamantan-1-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione542.3I-134 3-(5-(1-((1-cyclobutyl-1H-1,2,3-triazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione463.2I-128 3-(1-oxo-5-(1-(pyrazolo[1,5-a]pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione458.2I-205 3-(5-(1-(4-hydroxy-3-((4-methylpiperazin-1-yl)methyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione546.3I-59 3-(1-oxo-5-(1-(pyridin-3-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione419.2I-60 3-(1-oxo-5-(1-(pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione419.2I-58 3 -(1 -oxo-5 -(1 -(pyridin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione419.2

[0327] The following compounds in Table 2 were prepared from intermediate 1-155 and corresponding halide according to an alkylation procedure described in Example 8 (Method 2): Table 2: Cmpd No. Compound Name MS [M+1] I-204 3-(5-(1-((1,2,4-oxadiazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione410.2I-243 3-(1-oxo-5-(1-((5-(4-(trifluoromethyl)phenyl)-1,2,4-oxadiazol-3-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione554.2I-199 3-(1-oxo-5-(1-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine -2,6-dione486.2I-211 3-(1-oxo-5-(1-((2-phenylthiazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione501.2I-10 3-(5-(1-((2-chlorothiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione459.1I-238 3-(1-oxo-5-(1-(thiazol-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione425.2I-221 3-(5-(1-((7-methyl-4-oxo-4H-pyrido[1,2-a]pyrimidin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione500.2I-213 3-(1-oxo-5-(1-((4-oxo-3,4-dihydrothieno [3,2-d]pyrimidin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione492.2I-254 methyl 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)oxazole-4-carboxylate467.2I-246 3-(5-(1-(isoxazol-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione409.2I-245 3-(5-(1-((3-methylisoxazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione423.2I-258 3-(5-(1-((3-cyclohexylisoxazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione491.3I-203 3-(5-(1-((1-methyl-1H-indazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione472.2I-198 3-(5-(1-((1-benzyl-1H-tetrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione500.2I-217 6-((4-(2-(2,6-dioxopiperidm-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)picolinonitrile444.2I-219 3-(5-(1-((1H-indazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione458.2I-214 3-(1-oxo-5-(1-(quinolin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione469.2I-235 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-1,2,4-oxadiazole-5-carboxamide453.2I-200 3-(5-(1-(benzo[d]thiazol-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione475.2I-239 3-(1-oxo-5-(1-(quinoxalin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione470.2I-241 3-(1-oxo-5-(1-((3-(m-tolyl)-1,2,4-oxadiazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione500.2I-43 3-(5-(1-((1H-indazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione458.2I-232 3-(5-(1-((7-fluoroquinolin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione487.2I-207 3-(5-(1-((2-(4-chlorophenyl)-5-methyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione533.2I-251 3-(5-(1-((5-methyl-3-phenylisoxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione499.2I-226 3-(5-(1-(isoquinolin-1-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione469.2I-257 3-(5-(1-((5-methyl-2-phenyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione499.2I-56 3-(1-oxo-5-(1-((3-phenyl-1,2,4-oxadiazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione486.2I-208 3-(5-(1-((7-hydroxy-2-methylpyrazolo[1,5 -a]pyrimidin-5 - yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione489.2I-229 tert-butyl (5 -((4-(2-(2,6-dioxopiperidin-3 -yl)- 1 -oxoisoindolin-5 -yl)piperidin-1-yl)methyl)-4-(trifluoromethyl)thiazol-2-yl)carbamate608.2I-240 3-(5-(1-((2-(4-fluorophenyl)-5-methyloxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione517.2I-233 3-(5-(1-((5-methyl-2-(4-(trifluoromethyl)phenyl)oxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione567.2I-88 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione490.2I-64 3-(5-(1-(3,4-difluorobenzyl)piperidin-4-yl)-1-oxoisomdolin-2-yl)piperidine-2,6-dione454.2I-225 3-(5-(1-(4-(5-methylbenzo[d]thiazol-2-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione565.2I-38 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzonitrile443.2I-255 3-(1-oxo-5-(1-(4-(pyridin-2-ylmethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione525.3I-75 3-(1-oxo-5-(1-(4-propylbenzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione460.3I-244 3-(5-(1-(4-((4-fluorobenzyl)oxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione542.2I-74 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoic acid462.2I-248 3-(5-(1-(4-(methoxymethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione462.2I-79 3-(5-(1-(3-(difluoromethoxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione484.2I-77 3-(5-(1-(4-(difluoromethoxy)benzyl)piperidin-4-yl)- 1-oxoisoindolin-2-yl)piperidine-2,6-dione484.2I-32 3-(5-(1-(4-(hydroxymethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione448.2I-216 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-N,N-dimethylbenzenesulfonamide525.2I-76 3-(1-oxo-5-(1-(4-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione486.2I-29 3-(5-(1-(2,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione454.2I-91 3-(5-(1-(4-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione484.2I-36 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl) benzonitrile443.2I-70 3-(5-(1-(4-(difluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione468.2I-236 3-(5-(1-(3-(morpholinosulfonyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione567.2I-95 3-(1-oxo-5-(1-(pyrimidin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione420.2I-110 3-(5-(1-(4-cyclopentylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione486.3I-65 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)pyrimidine-5-carbonitrile445.2I-67 3-(5-(1-(2-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione448.2I-83 3-(5-(1-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione476.2I-252 3-(5-(1-(4-((difluoromethyl)sulfonyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione532.2I-87 3-(5-(1-((2,2-difluorobenzo[d][1,3]dioxol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione498.2I-69 3-(5-(1-(3-fluoro-4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione450.2I-68 3-(5-(1-((2-methoxypyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione450.2I-107 3-(5-(1-(3-(tert-butyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione474.3I-108 3-(5-(1-(3-isopropoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione476.31-78 3-(1-oxo-5-(1-((5 -(trifluoromethyl)pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione487.2I-89 3-(1-oxo-5-(1-(4-(tert-pentyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione488.3I-82 3-(5-(1-((2,3-dihydrobenzo[b][1,4]dioxin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione476.2I-93 3-(5-(1-(3-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione484.2I-206 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetonitrile457.2I-113 3-(5-(1-(2,4-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione486.1I-106 3-(1-oxo-5-(1-(2-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione486.2I-81 3-(5-(1-(4-cyclobutylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione472.3I-218 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenoxy)acetonitrile473.2I-104 3-(5-(1-(2-cyclopropylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione458.2I-101 3-(5-(1-(2,4-difluorobenzyl)pipendin-4-yl)-1-oxoisomdolin-2-yl)piperidine-2,6-dione454.2I-42 3-(5-(1-(2,4-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione446.2I-227 3-(5-(1-(4-(4-methoxypiperidin-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione531.3I-228 3-(5-(1-(4-(isopropylthio)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione492.2I-24 3-(5-(1-(2,6-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione454.2I-231 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetic acid476.2I-73 3-(5-(1-(3-(difluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione468.2I-237 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5 -yl)piperidin- 1-yl)methyl)-N,N-dimethylbenzenesulfonamide525.2I-63 3-(5-(1-(4-(fluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione450.2I-114 3 -(1-oxo-5-(1-(quinolin-8-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione469.2I-80 3-(5-(1-(2-(difluoromethoxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione484.2I-234 3-(5-(1-((2-amino-4-(trifluoromethyl)thiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione508.2 Example 9: 3-(5-(1-benzyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-172)

[0328]

[0329] A solution of 4N HCl in dioxane (2.0 mL) was added to 1e (300 mg, 0.71 mmol) in DCM (2.0 mL) at rt and the resulting mixture was stirred at rt for 2 h. The reaction mixture was then concentrated to dryness and the resulting residue was washed with ether, decanted, and then dried under high vacuum. The crude material was dissolved in DMF (3.0 mL) and then NEt 3 (0.46 mL, 2.48 mmol) was added. The mixture was cooled in an ice bath for 10 min prior to the dropwise addition of benzyl bromide (8b, 0.08 mL, 0.663 mmol). The resulting reaction mixture was then stirred at 0 °C for 1 h. The reaction mixture was then diluted with water and extracted with ethyl acetate. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered, and concentrated to dryness. The crude material was purified by reverse phase HPLC (MeCN / H 2 O). The fractions with the desired product were collected and concentrated to dryness affording I-172 as an off-white solid (30 mg, 0.071 mmol, 10% yield). MS [M+H] +< = 416.4. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.99 (s, 1H), 7.77-7.69 (m, 2H), 7.66-7.57 (m, 3H), 7.51 (d, J = 3.0 Hz, 3H), 6.32 (s, 1H), 5.12 (dd, J = 13.3, 5.1 Hz, 1H), 4.54-4.40 (m, 3H), 4.34 (d, J = 17.5 Hz, 1H), 3.83 (s, 2H), 3.65 (brs, 1H), 2.99-2.80 (m, 3H), 2.70-2.55 (m, 2H), 2.11-1.92 (m, 1H).Example 10: 3-(5-(1-(4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione HC(O)OH (I-112)

[0330]

[0331] To a solution of I-155 (60 mg, 0.17 mmol) and 4-fluorobenzaldehyde (10a, 0.05 mL, 0.5 mmol) in DMF (2 mL) was added sodium triacetoxyborohydride (105 mg, 0.495 mmol) in one portion and the resulting mixture was stirred vigorously at rt overnight. The reaction mixture was then concentrated under reduced pressure. The crude product was diluted with aqueous formic acid (0.1 M in H 2 O) and MeCN. The resulting solution was directly purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The pure fractions containing the desired product were combined, concentrated, and the product lyophilized to afford the formate salt of I-112 (41.0 mg, 0.094 mmol, 57% yield, formate salt) as a white solid. MS [M+H] +< = 436.4. 1< H NMR (400 MHz, D 2 O): δ 8.45 (s, 1H), 7.80 (d, J = 8.0 Hz, 1H), 7.60-7.43 (m, 4H), 7.26 (t, J = 8.7 Hz, 2H), 5.17 (dd, J = 13.3, 5.3 Hz, 1H), 4.60 (d, J = 17.4 Hz, 1H), 4.51 (d, J = 17.6 Hz, 1H), 4.36 (s, 2H), 3.66 (d, J = 12.3 Hz, 2H), 3.20 (t, J = 12.6 Hz, 2H), 3.07 (t, J = 12.5 Hz, 1H), 3.02-2.83 (m, 2H), 2.55 (qd, J = 12.9, 5.3 Hz, 1H), 2.35-2.24 (m, 1H), 2.19 (d, J = 14.2 Hz, 2H), 2.05-1.85 (m, 2H). 13< C NMR (100 MHz, DMSO-d 6 ) δ 172.91, 171.10, 168.04, 161.22 (d, J = 242 Hz), 150.63, 142.47, 134.70 (d, J = 3.0 Hz), 130.62 (d, J = 8.1 Hz), 129.76, 126.93, 122.92, 121.73, 114.85 (d, J = 20.8 Hz), 61.44, 53.40, 51.53, 47.10, 42.17, 33.05, 31.23, 22.52.Example 11: 3-(5-(1-(4-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-97)

[0332]

[0333] Compound I-97 was prepared from 1-155 (70 mg, 0.19 mmol) and 4-chlorobenzaldehyde (11a, 81 mg, 0.58 mmol) via reductive amination as described for Example 8. Upon completion of the reaction, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O then decanted. The remaining residue was then purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). Concentration of the solvent afforded the formate salt of I-97 as an off-white solid (18 mg, 0.036 mmol, 19% yield, formate salt). MS [M+H] +< = 452.4. 1< H NMR (CDCl 3 , 400 MHz): δ 7.90 (s, 1H), 7.79 (d, J = 8.0 Hz, 1H), 7.35-7.29 (m, 6 H), 5.23-5.19 (dd, J = 13.2 Hz, 5.2 Hz, 1H), 4.45 (d, J = 14 Hz, 1H), 4.3 (d, J = 16 Hz, 1H), 3.50 (s, 2H), 3.00-2.82 (m, 4H), 2.65-2.55 (m, 2H), 2.36-2.32 (m, 1H), 2.21-2.19 (m, 1H), 2.09-2.08 (m, 2H), 1.82-1.80 (m, 3H).Example 12: 3-(5-(1-(4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I158)

[0334]

[0335] Compound I-158 was prepared from I-155 (50 mg, 0.14 mmol) and 4-methylbenzaldehyde (12a, 20 mg, 0.16 mmol) via reductive amination as described for Example 8. After workup, the crude material was purified by silica gel chromatography eluting with 5% MeOH in DCM affording I-158 as an off-white solid (25.3 mg, 0.059 mmol, 42% yield). MS [M+H] +< = 432.5. 1< H NMR (DMSO-d 6 , 400 MHz): δ 11.0 (s, 1H), 7.69 (d, J = 8.0 Hz, 1H), 7.50 (d, J = 8.0 Hz, 2H), 7.45 (s, 1H), 7.38-7.36 (d, J = 8.0 Hz, 1H), 7.28 (d, J = 8.0 Hz, 2H), 5.11 (dd, J = 13.6, 5.2 Hz, 1H), 4.48 (d, J = 18.4 Hz, 1H), 4.32-4.28 (m, 3H), 3.43-3.40 (m, 2H), 3.03-2.86 (m, 4H), 2.73-2.57 (m, 2H), 2.42-2.34 (m, 4H), 2.11-1.96 (m, 4H).Example 13: 3-(5-(1-(3-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I173)

[0336]

[0337] Compound I-173 was prepared from I-155 (50 mg, 0.14 mmol) and 3-methylbenzaldehyde (13a, 20 mg, 0.16 mmol) via reductive amination as described for Example 8. After workup, the crude material was purified by reverse phase HPLC (MeCN / H 2 O with 0.05 formic acid). The fractions were collected and concentrated to dryness affording the formate salt of I-173 as an off-white solid (18.5 mg, 0.039 mmol, 27% yield, formate salt). MS [M+H] +< = 432.6. 1< H NMR (DMSO-d 6 , 600 MHz): δ 10.98 (s, 1H),8.14 (s, 1H), 7.64 (d, J = 7.9 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J = 7.9 Hz, 1H), 7.23 (t, J = 7.5 Hz, 1H), 7.18-7.11 (m, 2H), 7.08 (d, J = 7.4 Hz, 1H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.54 (s, 2H), 3.03-2.85 (m, 3H), 2.73-2.56 (m, 2H), 2.39 (dd, J = 13.0, 4.5 Hz, 1H), 2.31 (s, 3H), 2.15 (t, J = 10.7 Hz, 2H), 1.99 (dd, J = 9.0, 3.7 Hz, 1H), 1.83-1.67 (m, 4H).Example 14: 3-(5-(1-(2-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I157)

[0338]

[0339] Compound I-157 was prepared from I-155 (50 mg, 0.14 mmol) and 2-methylbenzaldehyde (14a, 20 mg, 0.16 mmol) via reductive amination as described for Example 8. After workup, the crude material was purified by silica gel chromatography eluting with 5% MeOH in DCM affording I-157 as a light brown solid (23.3 mg, 0.054 mmol, 39% yield). MS [M+H] +< = 432.5. 1< H NMR (400 MHz, DMSO-d 6 ) δ 10.98 (s, 1H), 7.77-7.15 (m, 7H), 5.11 (d, J = 13.5 Hz, 1H), 4.54-4.19 (m, 3H), 3.58-3.38 (m, 2H), 3.34 (s, 2H), 3.27-3.13 (m, 1H), 3.08-2.82 (m, 2H), 2.74-2.55 (m, 2H), 2.48-2.28 (m, 4H), 2.19-1.92 (m, 4H).Example 15: 3-(5-(1-(2,6-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-174)

[0340]

[0341] Compound 1-174 was prepared from I-155 (70 mg, 0.19 mmol) and 2,6-dimethylbenzaldehyde (15a, 77 mg, 0.57 mmol) via reductive amination as described for Example 8. After workup, the crude material was triturated with Et 2 O, EtOAc, and then heptane. The resultant solid was dried under high vacuum affording I-174 as a grey solid (17 mg, 0.038 mmol, 20% yield). MS [M+H] +< = 446.1. 1< H NMR (DMSO-d 6 , 400 MHz): δ 10.98 (s, 1H), 7.62 (d, J = 8.0 Hz, 1H), 7.47 (s, 1H), 7.37 (d, J = 8.0 Hz, 1H), 7.05-6.96 (m, 3H), 5.09 (dd, J = 13.2 Hz, 5.2 Hz, 1H), 4.40 (d, J = 16.8 Hz, 1H), 4.26 (d, J = 17.2 Hz, 1H), 3.47 (s, 2H), 2.93-2.84 (m, 3H), 2.66-2.60 (m, 1H), 2.40-2.36 (m, 7H), 2.20-2.14 (m, 2H), 1.98-1.95 (m, 1H), 1.76-1.73 (m, 2H), 1.63-1.58 (m, 2H).Example 16: 3-(5-(1-(3,5-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-159)

[0342]

[0343] Compound 1-159 was prepared from 1-155 (90 mg, 0.25 mmol) and 3,5-dimethylbenzaldehyde (16a, 110 mg, 0.82 mmol) via reductive amination as described for Example 8. After the reaction was complete, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O and then decanted. The remaining residue was dried under high vacuum affording 1-159 as a brown solid (70 mg, 0.16 mmol, 64% yield). MS [M+H] +< = 446.1. 1< H NMR (DMSO-d 6 , 400 MHz): δ 10.99 (s, 1H), 7.63 (d, J = 8.0 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 6.91 (s, 2H), 6.87-6.86 (m, 1H), 5.09 (dd, J = 13.2 Hz, 4.8 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.27 (d, J = 16 Hz, 1H), 3.41(s, 2H), 2.93-2.88 (m, 3H), 2.67-2.60 (m, 1H), 2.41-2.37 (m, 1H), 2.26-2.23 (s, 6H), 2.05-1.98 (m, 4H), 1.74-1.67 (m, 4H).Example 17: 3-(5-(1-(4-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I39)

[0344]

[0345] Compound 1-39 was prepared from I-155 (90 mg, 0.25 mmol) and p-anisaldehyde (17a, 40 mg, 0.30 mmol) via reductive amination as described for Example 8. After the reaction was complete, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O then decanted. The remaining residue was then purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). Removal of the solvent afforded the formate salt of I-39 as a yellow oil (40 mg, 0.081 mmol, 32% yield, formate salt). MS [M+H] +< = 448.4. 1< H NMR (DMSO-d 6 , 400 MHz): δ 10.98 (s, 1H), 8.14 (s, 1H), 7.63 (d, J = 8.0 Hz, 1H), 7.48 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 7.24 (d, J = 8.3 Hz, 2H), 6.89 (d, J = 8.8 Hz, 2H), 5.11 (dd, J = 13.3 Hz, 5.2 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.27 (d, J = 17.2 Hz, 1H), 3.73 (s, 3H), 3.47 (s, 2H), 2.95-2.87 (m, 3H), 2.67-2.61 (m, 2H), 2.40-2.32 (m, 1H), 2.10-2.05 (m, 2H), 1.99-1.97 (m, 1H), 1.78-1.60 (m, 4H).Example 18: 3-(5-(1-(4-nitrilebenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-31)

[0346]

[0347] Compound 1-31 was prepared from I-155 (90 mg, 0.25 mmol) and 4-formylbenzonitrile (18a, 97 mg, 0.74 mmol) via reductive amination as described for Example 8. After the reaction was complete, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Ft 2 O and then decanted. The remaining residue was dried under high vacuum affording I-31 as a grey solid (42 mg, 0.10 mmol, 38% yield). MS [M+H] +< = 443.1. 1< H NMR (DMSO-d 6 , 300 MHz): δ 10.99 (1H, s), 7.81 (d, J = 8.4 Hz, 2H), 7.64 (d, J = 7.6 Hz, 1H), 7.55 (d, J = 8.4 Hz, 2 H), 7.50 (1H, s), 7.41 (d, J = 8.0 Hz, 1H), 5.09 (dd, J = 13.6, 5.2 Hz, 1H), 4.42 (d, J = 16.8 Hz, 1H), 4.29 (d, J = 17.2 Hz, 1H), 3.60 (s, 2H), 2.94-2.88 (m, 2H), 2.66-2.52 (m, 2H), 2.40-2.32 (m, 2H), 2.13-2.08 (m, 2H), 1.99-1.96 (m, 1H), 1.78-1.70 (m, 4H).Example 19: 3-(5-(1-(4-ethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-66)

[0348]

[0349] Compound I-66 was prepared from I-155 (25 mg, 0.07 mmol) and 4-ethyl benzaldehyde (19a, 11 mg, 0.08 mmol) via reductive amination as described for Example 8. After workup, the crude material was purified by silica gel chromatography eluting with 5% MeOH in DCM affording I-66 as an off-white solid (11 mg, 0.025 mol, 35% yield). MS [M+H] +< = 446.5. 1< H NMR (400 MHz, DMSO-d 6 ) δ 10.97 (s, 1H), 7.63 (d, J = 7.9 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 7.7 Hz, 1H), 7.23 (d, J = 8.0 Hz, 2H), 7.16 (d, J = 7.9 Hz, 2H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.46 (s, 2H), 2.98-2.85 (m, 3H), 2.68-2.55 (m, 4H), 2.45-2.32 (m, 1H), 2.11-1.94 (m, 3H), 1.81-1.64 (m, 4H), 1.18 (t, J = 7.6 Hz, 3H).Example 20: 3-(5-(1-(4-isopropylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-45)

[0350]

[0351] Compound I-45 was prepared from I-155 (100 mg, 0.27 mmol), 1-(bromomethyl)-4-isopropylbenzene (20a, 58 mg, 0.27 mmol) and NEt 3 (0.077 mL, 0.55 mmol) similar to the alkylation procedure described in method 2 of Example 8. Upon work up the crude material was purified by reverse phase HPLC (MeCN / H 2 O with 0.05% formic acid). The fractions with the desired product were concentrated to dryness to afford the formate salt of I-45 as an off-white solid (18 mg, 0.036 mmol, 13% yield, formate salt). MS [M+H] +< = 460.5. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.63 (d, J = 7.9 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 6.8 Hz, 1H), 7.24 (d, J = 8.2 Hz, 2H), 7.19 (d, J = 8.2 Hz, 2H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.47 (s, 2H), 2.98-2.80 (m, 4H), 2.72-2.57 (m, 2H), 2.43-2.30 (m, 1H), 2.10-1.93 (m, 3H), 1.80-1.64 (m, 4H), 1.20 (d, J = 6.9 Hz, 6H).Example 21: 3-(5-(1-(4-(tert-butyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-84)

[0352]

[0353] Compound I-84 was prepared from I-155 (70 mg, 0.19 mmol) and 1-(bromomethyl)-4-(tert-butyl)benzene (21a, 43 mg, 0.19 mmol) and K 2 CO 3 (79 mg, 0.58 mmol) similar to the alkylation procedure described in method 2 of Example 8. The crude material, after trituration with Et 2 O, was purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid. The fractions containing the desired product were concentrated to dryness to afford the formate salt of I-84 as an off-white solid (25 mg, 0.048 mmol, 25% yield, formate salt). MS [M+H] +< = 474.4. 1< H NMR (400 MHz, DMSO-d 6 ) δ 10.98 (s, 1H), 8.18 (s, 1H), 7.64 (d, J = 7.8 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 7.9 Hz, 1H), 7.35 (d, J = 8.3 Hz, 2H), 7.25 (d, J = 8.2 Hz, 2H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.42 (d, J = 17.3 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.50 (s, 2H), 2.99-2.84 (m, 3H), 2.72-2.60 (m, 1H), 2.46-2.30 (m, 2H), 2.09 (t, J = 10.8 Hz, 2H), 2.03-1.93 (m, 1H), 1.82-1.65 (m, 4H), 1.28 (s, 9H).Example 22: 3-(5-(1-([1,1'-biphenyl]-4-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-90)

[0354]

[0355] Compound I-90 was prepared from I-155 (90 mg, 0.25 mmol) and [1,1'-biphenyl]-4-carbaldehyde (22a,135 mg, 0.74 mmol) via reductive amination as described for Example 8. After workup, the crude material was triturated with EtOAc and then filtered. The solid was dried under high vacuum affording I-90 as an off-white solid (45 mg, 0.09 mmol, 37% yield). MS [M+H] +< = 494.1. 1< H NMR (DMSO-d 6 , 400 MHz): δ 11.0 (s, 1H), 7.67-7.61 (m, 4H), 7.51-7.35 (m, 6H), 5.08 (dd, J = 13.2, 5.2 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.55 (s, 2H), 2.98-2.85 (m, 3H), 2.66-2.56 (m, 2H), 2.39-2.32 (m, 2H), 2.10 (m, 2H), 1.95 (m, 2H), 1.77-1.73 (m, 4H).Example 23: 3-(5-(1-(3,5-difluoro-4-hydroxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-156)

[0356]

[0357] To compound I-155 (15 mg, 0.041 mmol) and 3,5-difluoro-4-hydroxybenzaldehyde (23a, 20 mg, 0.13 mmol) in DCM (0.6 mL) and DMF (0.6 mL) was added sodium triacetoxyborohydride (26 mg, 0.12 mmol) in one portion and the resulting mixture was stirred vigorously for 12 h at rt. The reaction mixture was then concentrated under reduced pressure and the crude product was diluted with aqueous formic acid (0.1 M in H 2 O) and MeCN. The resulting solution was directly purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The pure fractions were combined and concentrated to dryness to afford the formate salt of I-156 (12.1 mg, 0.023 mmol, 57% yield, formate salt) as a white solid. MS [M+H] +< = 470.3. 1< H NMR (400 MHz, acetonitrile-d 3 ) δ 8.82 (s, 1H), 8.18 (s, 1H), 7.66 (s, 1H), 7.45 (d, J = 1.3 Hz, 1H), 7.39 (dd, J = 7.9, 1.4 Hz, 1H), 7.10-6.86 (m, 2H), 5.06 (dd, J = 13.4, 5.2 Hz, 1H), 4.38 (d, J = 16.7 Hz, 1H), 4.30 (d, J = 16.8 Hz, 1H), 3.49 (s, 2H), 2.99 (d, J = 11.5 Hz, 2H), 2.82 (ddd, J = 17.7, 13.3, 5.3 Hz, 1H), 2.77-2.62 (m, 2H), 2.41 (qd, J = 13.2, 4.8 Hz, 1H), 2.22-2.07 (m, 3H), 1.86-1.73 (m, 4H).Example 24: 3-(5-(1-((1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-118)

[0358]

[0359] To I-155 (15 mg, 0.041 mmol) and 1H-pyrazole-3-carbaldehyde (24a, 12 mg, 0.12 mmol) in DCM (0.6 mL) and DMF (0.6 mL) was added sodium triacetoxyborohydride (26 mg, 0.12 mmol) in one portion and the resulting mixture was stirred vigorously for 12 h at rt. The reaction mixture was concentrated under reduced pressure and the crude product was diluted with aqueous formic acid (0.1 M in H 2 O) and MeCN. The resulting solution was directly purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The pure fractions containing the desired product were combined and concentrated to dryness to afford the formate salt of I-118 (9.7 mg, 0.021 mmol, 52% yield, formate salt) as a white solid. MS [M+H] +< = 408.1. 1< H NMR (400 MHz, D 2 O): δ 8.44 (s, 1H), 7.82 (d, J = 2.4 Hz, 1H), 7.77-7.71 (m, 1H), 7.52 (s, 1H), 7.45 (dd, J = 8.0, 1.5 Hz, 1H), 6.59 (d, J = 2.4 Hz, 1H), 5.13 (dd, J = 13.3, 5.2 Hz, 1H), 4.56 (d, J = 17.6 Hz, 1H), 4.47 (d, J = 17.6 Hz, 1H), 4.40 (s, 2H), 3.64 (d, J = 12.3 Hz, 2H), 3.19 (t, J = 12.6 Hz, 2H), 3.08-2.81 (m, 3H), 2.51 (qd, J = 12.9, 5.4 Hz, 1H), 2.24 (dtd, J = 13.0, 5.2, 2.7 Hz, 1H), 2.14 (d, J = 14.3 Hz, 2H), 2.05-1.89 (m, 2H).Example 25: 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-164)

[0360]

[0361] Compound 1-164 was prepared from 1-155 (60 mg, 0.16 mmol) and 5,6,7,8-tetrahydronaphthalene-1-carbaldehyde (25a, 78 mg, 0.48 mmol) via reductive amination as described for Example 8. After reaction was complete, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O then decanted. The remaining residue was then purified by reverse phase HPLC (MeCN / H 2 O with 0.02% NH 4 OH) and the desired fractions concentrated to dryness to afford I-164 as brown solid (6 mg, 0.012 mmol, 8% yield). MS [M+H] +< = 472.4. 1< H NMR (CDCl 3 , 600 MHz): δ 7.92 (brs, 1H), 7.81-7.80 (d, J = 6 Hz, 1H), 7.37-7.33 (m, 2H), 7.16-7.12 (m, 1H), 7.10-7.04 (m, 1H), 7.03-7.01 (m, 1H), 5.26-5.20 (m, 1H) 4.46 (d, J = 15.6 Hz, 1H), 4.30-4.33 (d, J = 16.2 Hz, 1H), 3.44 (s, 2H), 3.02 (m, 2H), 2.91 (d, 1H), 2.80-2.83 (m, 6H), 2.22 (m, 1H), 2.10 (m, 2H), 1.82-1.80 (m, 9H).Example 26: 3-(1-oxo-5-(1-((1,2,3,4-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (1-168)

[0362]

[0363] Compound I I-168 was prepared from I-155 (60 mg, 0.16 mmol) and 1,2,3,4-tetrahydronaphthalene-1-carbaldehyde (26a, 78 mg, 0.48 mmol) via reductive amination as described for Example 8. Upon completion of the reaction, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O then decanted. The remaining residue was then purified by reverse phase HPLC (MeCN / H 2 O) and the desired fractions were concentrated to dryness to afford I-168 as a brown solid (10 mg, 0.02 mmol, 14% yield). MS [M+H] +< = 472.4. 1< H NMR (CDCl 3 , 300 MHz): δ 7.92 (s, 1H), 7.82 (d, J = 7.5 Hz, 1H), 7.39-7.36 (m, 2H), 7.26-7.23 (s, 1H), 7.13-7.06 (m, 3H), 5.23 (dd, J = 12.9, 5.1 Hz, 1H), 4.48 (d, J = 15.9 Hz, 1H), 4.32 (d, J = 15.6 Hz, 1H), 3.23-3.19 (m, 1H), 3.00-2.77 (m, 7H), 2.63-2.54 (m, 3H), 2.39-2.35 (m, 1H), 2.24-2.17 (m, 2H), 1.98-2.08 (m, 3H), 1.26-1.86 (m, 4H).Example 27: 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-175)

[0364]

[0365] Compound I-175 was prepared from I-155 (100 mg, 0.27 mmol) and 5,6,7,8-tetrahydronaphthalene-2-carbaldehyde (27a, 49 mg, 0.30 mmol) via reductive amination as described for Example 8. Upon completion of the reaction, the crude reaction mixture was concentrated to dryness. The resulting material was triturated with Et 2 O then decanted. The remaining residue was then purified by reverse phase HPLC (MeCN / H 2 O with 0.02% NH 4 OH). Concentration to dryness of the desired fractions afforded I-175 as an off-white solid (18 mg, 0.038 mmol, 14% yield). MS [M+H] +< = 472.4. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 8.16 (s, 1H), 7.63 (d, J = 7.9 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 7.03-6.96 (m, 3H), 5.10 (dd, J = 13.3, 5.1 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.3 Hz, 1H), 3.43 (s, 2H), 2.97-2.88 (m, 3H), 2.76-2.59 (m, 6H), 2.45-2.30 (m, 1H), 2.12-1.92 (m, 3H), 1.77-1.69 (m, 7H).Example 28: Preparation of Intermediate 29f: (2S)-tert-butyl 2-methyl-4-(tosyloxy)piperidine-1-carboxylate (29f)

[0366] Step 1. tert-butyl (2S)-4-hydroxy-2-methylpiperidine-1-carboxylate

[0367] To (S)-tert-butyl 2-methyl-4-oxopiperidine-1-carboxylate (28a, 1.0 g, 4.7 mmol) in MeOH (5 mL) was added NaBH 4 (213 mg, 5.63 mmol) portionwise, and the reaction mixture was stirred overnight at rt. The reaction mixture was then quenched with brine and extracted with DCM. The combined organic phases were concentrated to afford 28b (957 mg, 4.45 mmol, 95% yield, 1:0.6 mixture of diastereomers). The compound was sufficiently pure to use in the next step without further purification. 1< H NMR of major diastereomer (400 MHz, CDCl 3 ): δ 4.28 (quintd, J = 6.8, 2.3 Hz, 1H), 4.17 (quint, J = 3.4 Hz, 1H), 3.82 (ddd, J = 13.5, 4.9, 2.8 Hz, 1H), 3.25 (ddd, J = 13.5, 11.8, 4.0 Hz, 1H), 1.85-1.79 (m, 1H), 1.69-1.64 (m, 2H), 1.55-1.51 (m, 1H), 1.46 (s, 9H), 1.32 (d, J = 7.1 Hz, 3H), 1.40 -1.26 (m, 1H). 1H NMR of minor diastereomer (400 MHz, CDCl 3 ) δ 4.55-4.44 (m, 1H), 4.04 (d, J = 14.2 Hz, 1H), 3.95 (tt, J = 11.3, 4.4 Hz, 1H), 2.87 (td, J = 13.5, 2.8 Hz, 1H), 1.93 (ddq, J = 12.3, 5.0, 2.6 Hz, 1H), 1.88-1.84 (m, 1H), 1.77-1.70 (m, 1H), 1.62 (dt, J = 3.8, 2.0 Hz, 1H), 1.51 (s, 1H), 1.45 (s, 9H), 1.14 (d, J = 7.0 Hz, 3H).Step 2. (2S)-tert-butyl 2-methyl-4-(tosyloxy)piperidine-1-carboxylate (29f)

[0368] To 28b (500 mg, 2.28 mmol), TEA (0.65 mL, 4.64 mmol) and DMAP (57 mg, 0.46 mmol) in MeCN (4 mL) was added TsCl (531 mg, 2.79 mmol) in one portion and the resulting mixture was stirred for 48 h at rt. After this time the reaction mixture was quenched with saturated aqueous NaHCO 3 and extracted with DCM (3x). The combined organic phases were dried over Na 2 SO 4 and concentrated under reduced pressure. The crude product was then purified via chromatography on silica gel eluting with 0 to 40% EtOAc in heptane to afford 29f (597 mg, 1.62 mmol, 70% yield, 1:0.7 mixture of diastereoisomers) as a slightly yellow solid. MS [M-56+H] +< = 314.2 and [M+Na] +< = 392.3. 1< H NMR of a 1:0.7 mixture of diastereoisomers (400 MHz, CDCl 3 ): δ 7.87-7.72 (m, 3.4H), 7.42-7.30 (m, 3.4H), 4.84 (quint, J = 3.1 Hz, 1H), 4.75 (hept, 0.7H), 4.51-4.42 (m, 0.7H), 4.36-4.23 (m, 1H), 4.01 (d, J = 14.2 Hz, 1H), 3.90-3.78 (m, 1H), 3.11 (td, J = 13.5, 2.8 Hz, 1H), 2.83 (td, J = 13.6, 2.8 Hz, 0.7H), 2.45 (s, 5.1H), 1.91 (ddt, J = 12.0, 4.6, 2.5 Hz, 1H), 1.85-1.69 (m, 4.5H), 1.66-1.60 (m, 1H), 1.55-1.50 (m, 0.6H), 1.43 (s, 9H), 1.43 (s, 6H), 1.23 (d, J = 7.1 Hz, 3H), 1.08 (d, J = 7.1 Hz, 2H).Example 29: 3-(5-((2S)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione HC(O)OH salt (I-160)

[0369] Step 1. 3-(5-iodo-1-oxoisoindolin-2-yl)piperidine-2,6-dione (29b)

[0370] To methyl 4-iodo-2-methylbenzoate (29a, 170 g, 615.78 mmol) in MeCN (1 L) was added AIBN (10.1 g, 61.51 mmol), and NBS (131.56 g, 739.18 mmol). The resulting solution was stirred overnight at 80 °C. The reaction mixture was cooled to rt and the solids were filtered out. The resulting mixture was concentrated under vacuum and was applied onto a silica gel column with ethyl acetate / petroleum ether (0-10%). The collected fractions were concentrated under vacuum to afford 29b (50 g, 140.9 mmol, 23% yield) as a white solid. 1< H NMR (300 MHz, DMSO-d 6 ) δ 8.04-8.01 (m, 1H), 7.88-7.81 (m, 1H), 7.67-7.59 (m, 1H), 4.96 (s, 2H), 3.87 (s, 3H).Step 2. 3-(5-iodo-1-oxoisoindolin-2-yl)piperidine-2,6-dione (29d)

[0371] To 29b (50 g, 140.86 mmol) was added 3-aminopiperidine-2,6-dione TFA salt (29c, 34.18 g, 141.15 mmol), DMF (500 mL), and TEA (42.4 g, 419.01 mmol). The resulting solution was stirred for 48 h at 60 °C. The reaction mixture was then cooled to rt and quenched by the addition of 500 mL of water / ice. The pH value of the solution was adjusted to 5 with HCl (1 M). The resulting solids were collected by filtration, washed with EtOAc, and dried to afford 29d (13 g, 35.1 mmol, 25% yield) as a gray solid. [M+H] +< = 371.0. 1< H NMR (300 MHz, DMSO-d 6 ) δ 11.00 (s, 1H), 8.06 (s, 1H), 7.90 (d, J = 7.8Hz, 1H), 7.52 (d, J = 7.8 Hz, 1H), 5.14-5.08 (m, 1H), 4.47-4.28 (m, 2H), 2.97-2.85 (m, 1H), 2.73-2.01 (m, 2H), 1.98-1.20 (m, 1H).Step 3. tert-Butyl-(2S)-4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)-2-methylpiperidine-1-carboxylate (29g):

[0372] To 29d (20 mg, 0.054 mmol), 29f (24 mg, 0.065 mmol), NiBr 2 •DME (1.7mg, 5.4 µmol), di-t-Bu-bipy (1.5 mg, 5.4 µmol), KI (9 mg, 0.05 mmol), and manganese powder (6 mg, 0.1 mmol) under a nitrogen atmosphere was added DMA (0.27 mL) followed by 4-ethylpyridine (6.2 µL, 0.054 mmol) and the reaction mixture was stirred vigorously at 80 °C overnight. The reaction mixture was then diluted with MeCN and filtered through a short pad of Celite ®< filter aid eluting with MeCN. The obtained solution was concentrated by azeotroping with heptane. The crude product was purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The pure fractions were combined, concentrated to dryness to afford 29g (11 mg, 0.026 mmol, 47% yield) as a white solid. MS [M-56+H] +< = 386.4 and [M+H] +< = 442.5. 1< H NMR (400 MHz, CDCl 3 ) δ 8.27 (s, 1H), 7.81 (d, J = 7.9 Hz, 1H), 7.33 (d, J = 8.1 Hz, 1H), 7.30 (s, 1H), 5.33-5.09 (m, 1H), 4.69-4.47 (m, 1H), 4.46 (d, J = 15.8 Hz, 1H), 4.31 (d, J = 15.9 Hz, 1H), 4.23-3.94 (m, 1H), 3.12-2.73 (m, 4H), 2.34 (dq, J = 14.6, 9.6 Hz, 1H), 2.20 (s, 1H), 1.84 (td, J = 13.4, 5.3 Hz, 2H), 1.70 (d, J = 13.8 Hz, 1H), 1.48 (s, 9H), 1.38 (s, 1H), 1.24 (d, J = 6.9 Hz, 3H)Step 4. 3-(5-((2S)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (29h)

[0373] To 29g (11 mg, 0.025 mmol) in THF (1 mL) was added 4 M HCl in dioxane (0.7 mL, 2.8 mmol) and the reaction mixture was stirred for 2 h at 60 °C. Formation of a white precipitate was observed. The reaction mixture was then diluted with Et 2 O and filtered. The precipitate was washed with Et 2 O and then dried to afford the hydrochloride salt of 29h (9 mg, 0.024 mmol, 97% yield, hydrochloride salt) as a white solid. MS [M+H] +< = 342.2. The compound was used in the next step without further purification.Step 5. 3-(5-((2S)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione HC(O)OH salt (I-160)

[0374] To 29h (9 mg, 0.024 mmol) and benzaldehyde (8 µL, 0.08 mmol) in DMF (1 mL) was added sodium triacetoxyborohydride (15 mg, 0.071 mmol) in one portion and the reaction mixture was stirred vigorously at rt overnight. The reaction mixture was then stirred for an additional 8 h at 60 °C. The resulting mixture was concentrated under reduced pressure and the crude product was diluted with aqueous formic acid (0.1 M in H 2 O) and MeCN. The resulting solution was directly purified by reverse phase HPLC (MeCN / H 2 O with 0.1% formic acid). The two fractions obtained were concentrated to dryness separately. The first fraction afforded a diastereomeric mixture containing I-160 (1.9 mg, 4.0 µmol, 17% yield, 10:1 mixture of diastereoisomers, formate salt) as a white solid. The second fraction afforded I-160 (1.2 mg, 2.5 µmol, 10% yield, single diastereomer, formate salt) as a white solid. Overall yield: 27% yield. MS [M+H] +< = 432.3. Major diastereomer 1< H NMR (400 MHz, acetonitrile-d 3 ): δ 8.72 (s, 1H), 8.04 (s, 1H), 7.58 (d, J = 7.9 Hz, 1H), 7.43-7.37 (m, 3H), 7.33 (dd, J = 7.9, 1.4 Hz, 1H), 7.27 (t, J = 7.3 Hz, 2H), 7.23-7.17 (m, 1H), 4.97 (dd, J = 13.4, 5.2 Hz, 1H), 4.30 (d, J = 16.7 Hz, 1H), 4.22 (d, J = 16.7 Hz, 1H), 3.77-3.65 (m, 2H), 3.29-3.17 (m, 1H), 3.06-2.94 (m, 1H), 2.78-2.59 (m, 4H), 2.06-2.00 (m, 3H), 1.75-1.61 (m, 3H), 1.14 (d, J = 6.8 Hz, 3H). 1< H NMR of minor diastereomer was not obtained due to insufficient material.Example 30: 3-(5-(1-isobutylpiperidin-4-yl)-1-oxoisoindolin-2-yl) piperidine-2,6-dione (I-4)

[0375]

[0376] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.52 mmol) in DMF (2 mL) was added isobutyl bromide (0.06 mL, 0.60 mmol) and the resulting mixture was stirred at 80 °C for 16 h. The reaction mixture was then cooled to rt and quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford 1-4 as off-white solid (45 mg, 0.11 mmol, 38% yield). MS [M+H] +< = 384.1. 1H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.63 (d, J = 8.0 Hz, 1H), 7.50 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.09 (dd, J = 13.2, 5.2 Hz, 1H), 4.40 (d, J = 17.2 Hz, 1H), 4.27 (d, J = 17.2 Hz, 1H), 2.94-2.86 (m, 3H), 2.67-2.57 (m, 2H), 2.45-2.32 (m, 2H), 2.06 (d, J = 7.2 Hz, 2H), 1.99-1.96 (m, 3H), 1.94-1.67 (m, 4H), 0.87 (d, J = 6.8 Hz, 6H).Example 31: 3-(5-(1-(cyclobutylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I 5)

[0377]

[0378] To a stirred solution of I-155 (75 mg, 0.23 mmol) and Et 3 N (0.16 mL, 1.14 mmol) in DMF (2 mL) was added (bromomethyl)cyclobutane (0.05 mL, 0.46 mmol) and the resulting mixture was stirred at 80 °C for 16 h. The reaction mixture was then cooled to rt and quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-5 as off-white solid (20 mg, 0.05 mmol, 22% yield). MS [M+H] +< = 396.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.63 (d, J = 8.0 Hz, 1H), 7.48 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 4.8 Hz, 1H) 4.42 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 2.94-2.86 (m, 3H), 2.65-2.55 (m, 2H), 2.43-2.32 (m, 1H), 2.35 (d, J = 7.2 Hz, 2H), 2.04-1.96 (m, 5H), 1.86-1.62 (m, 7H).Example 32: 3-(5-(1-(cyclopropylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-3)

[0379]

[0380] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added (bromomethyl)cyclopropane (0.06 mL, 0.61 mmol) and the resulting mixture was stirred at 80 °C for 16 h. The reaction mixture was then cooled to rt and quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-3 as off-white solid (20 mg, 0.05 mmol, 17% yield). MS [M+H] +< = 382.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.65 (d, J = 8.0 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.83-5.79 (m, 1H), 5.12-5.09 (m, 2H), 5.01 (d, J= 9.6 Hz, 1H), 4.43 (d, J = 17.2 Hz, 1H), 4.29 (d, J = 17.2 Hz, 1H), 3.18-3.02 (m, 2H), 2.91-2.88 (m, 2H), 2.67-2.51 (m, 2H), 2.41-2.40 (m, 1H), 2.38-2.36 m, 1H), 2.30-2.20 (m, 3H), 2.01-1.97 (m, 2H), 1.97-1.65 (m, 4H).Example 33: 3-(1-oxo-5-(1-((tetrahydro-2H-pyran-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-13)

[0381]

[0382] To a stirred solution of I-155 (100 mg, 0.23 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added 4-(bromomethyl)tetrahydro-2H-pyran (0.08 mL, 0.61 mmol) and the resulting mixture was heated to 80 °C for 16 h. The reaction mixture was then cooled to rt and quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford 1-13 as an off-white solid (10 mg, 0.02 mmol, 8% yield). MS [M+H] +< = 426.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.66 (d, J = 8.0 Hz, 1H), 7.48 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 4.8 Hz, 1H), 4.43 (d, J = 17.2 Hz, 1H), 4.30 (d, J = 17.2 Hz, 1H), 3.84 (d, J = 9.6 Hz, 2H), 3.31-3.27 (m, 1H), 2.95-2.86 (m, 3H), 2.67-2.57 (m, 3H), 2.43-2.3 (m, 2H), 2.17-2.13 (m, 2H), 2.00-1.97 (m, 3H), 1.90-1.62 (m, 6H), 1.18-1.58 (m, 2H).Example 34: 3-(1-oxo-5-(1-phenethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-14)

[0383]

[0384] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added (2-bromoethyl)benzene (0.08 mL, 0.61 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was then quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-14 as an off-white solid (15 mg, 0.03 mmol, 11% yield). MS [M+H] +< = 432.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 7.30-7.23 (m, 4H), 7.20-7.16 (m, 1H), 5.09 (dd, J= 13.2, 5.2 Hz, 1H), 4.42 (d, J = 17.2 Hz, 1H), 4.29 (d, J = 17.2 Hz, 1H), 3.08-3.05 (m, 2H), 2.88 (m, 1H), 2.88-2.67 (m, 2H), 2.63-2.50 (m, 4H), 2.41-2.38 (m, 1H), 2.11-2.09 (m, 2H), 2.00-1.98 (m, 1H), 1.97-1.68 (m, 4H).Example 35: 3-(1-oxo-5-(1-(4-(trifluoromethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-51)

[0385]

[0386] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added 2-(bromomethyl)-5-(trifluoromethoxy)benzene (0.09 mL, 0.61 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-51 as an off-white solid (80 mg, 0.16 mmol, 52% yield). MS [M+H] +< = 502.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J= 8.0 Hz, 1H), 7.45 (d, J= 8.4 Hz, 2H), 7.40 (d, J= 8.0 Hz, 1H), 7.32 (d, J = 8.4 Hz, 2H) 5.10 (dd, J = 13.2, 5.2 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.30 (d, J = 17.2 Hz, 1H), 3.08 (s, 2H), 2.93-2.86 (m, 3H), 2.67-2.59 (m, 2H), 2.45-2.33 (m, 1H), 2.12-2.11 (m, 2H), 2.00-1.97 (m, 1H), 1.80-1.67 (m, 4H).Example 36: 3-(1-oxo-5-(1-(3-(trifluoromethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-54)

[0387]

[0388] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added 1-(bromomethy1)-3-(trifluoromethoxy)benzene (0.09 mL, 0.61 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-54 as an off-white solid (65 mg, 0.13 mmol, 42% yield). MS [M+H] +< = 502.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.50-7.47 (m, 2H), 7.45-7.36 (m, 2H), 7.31 (brs, 1H), 7.25 (d, J = 7.6 Hz, 1H), 5.11 (dd, J = 13.2, 5.2 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.29 (d, J = 17.2 Hz, 1H), 3.58 (s, 2H), 2.94-2.90 (m, 3H), 2.67-2.62 (m, 2H), 2.41-2.33 (m, 1H), 2.13-2.08 (m, 2H), 2.00-1.98 (m, 1H), 1.79-1.70 (m, 4H).Example 37: 3-(5-(1-(3,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I26)

[0389]

[0390] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added 1-(bromomethyl)-3,5-difluorobenzene (0.07 mL, 0.61 mmol) ) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford 1-26 as an off-white solid (65 mg, 0.14 mmol, 47% yield). MS [M+H] +< = 454.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.95 (s, 1H), 7.62 (d, J= 8.0 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 7.10-7.04 (m, 3H), 5.09 (dd, J = 13.2, 5.2 Hz, 1H), 4.41 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 3.53 (s, 2H), 2.92-2.66 (m, 3H), 2.65-2.49 (m, 2H), 2.44-2.31 (m, 1H), 2.12-2.07 (m, 2H), 2.00-1.96 (m, 1H), 1.76-1.71 (m, 4H).Example 38: 3-(5-(1-ethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (1-1)

[0391]

[0392] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added ethyl bromide (0.04 mL, 0.61 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford 1-1 as an off-white solid (21 mg, 0.06 mmol, 20% yield). MS [M+H] +< = 356.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H) 4.43 (d, J = 17.2 Hz, 1H), 4.30 (d, J = 17.2 Hz, 1H), 3.00-2.86 (m, 3H), 2.67-2.54 (m, 2H), 2.50-2.33 (m, 2H), 2.00-1.97 (m, 2H), 1.79-1.65 (m, 4H), 1.02 (t, J = 7.2 Hz, 3H).Example 39: cis-3-(1-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-276).

[0393] Step 1. cis-(4-(trifluoromethyl)cyclohexyl)methanol (39b)

[0394] To a stirred solution of 39a (1.0 g, 5.2 mmol) in THF (20 mL) was added LiAlH 4 (400 mg, 10.30 mmol) in small portions at 0 °C and stirred for 2 h. The reaction mixture was quenched with 10% aq. NaOH and then stirred at rt for 1 h. The reaction mixture was filtered through a pad of Celite ®< filter aid and washed with EtOAc. The combined filtrate was dried over Na 2 SO 4 . filtered, and concentrated to dryness to afford 39b as a viscous oil (500 mg, 2.74 mmol, 54% yield). The product was used in the next step without further purification.Step 2. cis-4-(trifluoromethyl)cyclohexane-1-carbaldehyde (39c)

[0395] To a stirred solution of 39b (500 mg, 2.74 mmol) in DCM (20 mL) was added DMP (2.33 g, 5.49 mmol) at 0 °C and the resulting mixture was stirred at rt for 3 h. The reaction mixture was diluted with DCM (20 mL), washed with 10% aq. NaHCO 3 (2 x 25 mL) and brine (25 mL), dried over Na 2 SO 4 . filtered, and concentrated to dryness. The crude material was purified by silica gel chromatography eluting with 15% EtOAc in hexane to afford 39c as a pale yellow viscous oil (180 mg, 1.00 mmol, 41% yield). 1< H NMR (400 MHz, DMSO-d 6 ): δ 9.70 (s, 1H), 2.48-2.47 (m, 1H), 2.32-2.27 (m, 2H), 1.86-1.80 (m, 2H), 1.65-1.53 (m, 2H), 1.40-1.25 (m, 3H).Step 3. cis-3-(2-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-276)

[0396] To a stirred solution of I-155 (150 mg, 0.41 mmol) and 39c (165 mg, 0.91 mmol) in DMF (5 mL) was added NaBH(OAc) 3 (290 mg, 1.37 mmol) and the resulting mixture was stirred at 60 °C for 16 h. The reaction mixture quenched with ice-cold water and washed with EtOAc (2 x 25 mL). The aq. layer was basified with NaHCO 3 and extracted with 5%MeOH in DCM (2 x 25 mL). The combined organic extracts were dried over Na 2 SO 4 . filtered, and concentrated to dryness. The crude material was purified by silica gel chromatography eluting with 5% MeOH in DCM to afford I-276 as an off-white solid (24 mg, 0.05 mmol, 12% yield). MS [M+H] +< = 492.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.99 (s, 1H), 7.62 (d, J= 8.0 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H), 4.4 (d, J = 17.2 Hz, 1H), 4.28 (d, J = 17.2 Hz, 1H), 2.95-2.85 (m, 3H), 2.64-2.55 (m, 2H), 2.42-2.35 (m, 2H), 2.26-2.22 (m, 2H), 2.05-1.95 (m, 3H), 1.90-1.85 (m, 2H), 1.79-1.70 (m, 3H), 1.68-1.55 (m, 4H), 1.52-1.45 (m, 4H).Example 40: ethyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate (I-176)

[0397]

[0398] To a stirred solution of I-155 (100 mg, 0.30 mmol) and Et 3 N (0.21 mL, 1.53 mmol) in DMF (2 mL) was added ethyl-2-bromoacetate (0.06 mL, 0.61 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water and extracted with EtOAc (2 x 50 ml). The combined organic extracts were washed with brine (25 mL), dried over Na 2 SO 4 . and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 5% MeOH in DCM. The pure fractions were evaporated and triturated with diethyl ether to afford 1-176 as an off-white solid (20 mg, 0.05 mmol, 16% yield). MS [M+H] +< = 414.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.64 (d, J= 8.0 Hz, 1H), 7.50 (s, 1H), 7.40 (d, J= 8.0 Hz, 1H), 5.11 (dd, J =13.2, 5.2 Hz, 1H), 4.40 (d, J= 17.2 Hz, 1H), 4.30 (d, J = 17.2 Hz, 1H), 4.10 (q, J = 6.8 Hz, 2H), 3.25 (s, 2H), 2.94-2.85 (m, 3H), 2.69-2.56 (m, 2H), 2.43-2.33 (m, 2H), 2.09-1.96 (m, 2H), 1.78-1.65 (m, 4H), 1.20 (t, J = 6.8 Hz, 3H).Example 41: 3-(5-(1-((1H-indazol-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-137)

[0399]

[0400] To a stirred solution of I-155 (450 mg, 1.24 mmol)and Et 3 N (0.95 mL, 6.87 mmol) in DMF (5 mL) was added 6-(bromomethyl)-1H-indazole HBr salt (0.54 g, 1.86 mmol) [prepared following Heterocyclic Communications, 2015, 21, 5-8] and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The resulting solid was filtered, washed with water, and dried under reduced pressure to afford I-137 as an off-white solid (21 mg, 0.06 mmol, 20% yield). MS [M+H] +< = 458.3. 1< H NMR (400 MHz, DMSO-d 6 ): δ 12.95 (s, 1H), 10.96 (s, 1H), 8.01 (s, 1H), 7.69 (d, J = 8.0 Hz, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.50 (s, 1H), 7.46 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 7.11 (d, J = 8.4 Hz, 1H), 5.10 (dd, J= 13.2, 5.2 Hz, 1H), 4.41 (d, J= 17.2 Hz, 1H), 4.28 (d, J= 17.2 Hz, 1H), 3.63 (s, 2H), 2.90-2.87 (m, 3H), 2.66-2.57 (m, 2H), 2.40-2.36 (m, 1H), 2.14-2.08 (m, 2H), 2.00-1.97 (m, 1H), 1.76-1.70 (m, 4H).Example 42: tert-butyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate (I-177)

[0401]

[0402] To a stirred solution of I-155 (200 mg, 0.61 mmol) and Et 3 N (0.42 mL, 3.05 mmol) in DMF (2 mL) was added tert-butyl-2-bromo acetate (0.18 mL, 1.22 mmol) and the resulting mixture was stirred at rt for 16 h. The reaction mixture was quenched with ice-cold water. The solid was filtered, washed with water, and dried under reduced pressure to afford 1-177 as an off-white solid (135 mg, 0.30 mmol, 50% yield). MS [M+H] +< = 442.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J = 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H), 4.43 (d, J = 17.2 Hz, 1H), 4.30 (d, J = 17.2 Hz, 1H), 3.13 (s, 2H), 2.95-2.87 (m, 3H), 2.67-2.57 (m, 2H), 2.33-2.28 (m, 3H), 2.00-1.98 (m, 1H), 1.74-1.68 (m, 4H), 1.43 (s, 9H).Example 43: 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetic acid hydrochloride (I-178)

[0403]

[0404] To a solution of I-177 (130 mg, 0.29 mmol) in DCM (2 mL) was added 2M HCl in diethyl ether (0.5 mL) dropwise at 0 °C and the resulting mixture was stirred at rt for 16 h. Upon complete consumption of the starting material, the solvent was evaporated and the crude material was dried under reduced pressure. The resulting solid was triturated with hexane followed by diethyl ether, collected by filtration, and dried under reduced pressure to afford I-178 as an off-white solid (60 mg, 0.15 mmol, 49% yield). MS [M+H] +< = 386.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 13.98 (brs, 1H), 10.98 (s, 1H), 7.71 (d, J= 8.0 Hz, 1H), 7.50 (s, 1H), 7.42 (d, J = 8.0 Hz, 1H), 5.12 (dd, J = 13.2, 5.2 Hz, 1H), 4.45 (d, J = 17.2 Hz, 1H), 4.33 (d, J = 17.2 Hz, 1H), 4.16 (s, 2H), 3.62-3.59 (m, 2H), 3.33-3.18 (m, 3H), 2.96-2.92 (m, 2H), 2.62-2.51 (m, 1H), 2.50-2.38 (m, 1H), 2.10-1.98 (m, 4H).Example 44: 3-(1-oxo-5-(1-(3,3,3-trifluoropropyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione (I-179)

[0405]

[0406] To a stirred solution of 1-155 (200 mg, 0.61 mmol) and 3,3,3-trifluoropropanal (0.15 mL, 1.83 mmol) in DMF (2 mL) was added NaBH(OAc) 3 (390 mg, 1.82 mmol) in small portions at 0 °C and the resulting mixture was stirred for 48 h at rt. The reaction mixture was quenched with water and extracted with EtOAc (2 x 50 mL). The combined organic extracts were washed with brine (25 mL), dried over Na 2 SO 4 . and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 10% MeOH in DCM. The pure fractions were evaporated and triturated with diethyl ether to afford I-179 as an off-white solid (115 mg, 0.27 mmol, 44% yield). MS [M+H] +< = 424.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 7.64 (d, J =8.0 Hz, 1H), 7.49 (s, 1H), 7.39 (d, J= 8.0 Hz, 1H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H), 4.45 (d, J = 17.2 Hz, 1H), 4.36 (d, J = 17.2 Hz, 1H), 3.30-2.86 (m, 3H), 2.67-2.55 (m, 4H), 2.41-2.33 (m, 2H), 2.10-1.97 (m, 4H), 1.90-1.64 (m, 4H).Example 45: 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)-N-phenylacetamide (I-180)

[0407]

[0408] To a stirred solution of I-155 (200 mg, 0.61 mmol) and Et 3 N (0.42 mL, 3.05 mmol) in DMF (2 mL) was added 2-bromo-N-phenylacetamide (0.19 g, 0.91 mmol) [prepared following JMC, 2016, 59, 6709-6728] and the resulting mixture was stirred at rt for 6 h. The reaction mixture was quenched with ice-cold water. The solid was filtered, washed with water, and dried under reduced pressure to afford I-180 as an off-white solid (60 mg, 0.13 mmol, 21% yield). MS [M+H] +< = 461.2. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.96 (s, 1H), 9.70 (s, 1H), 7.66 (d, J = 8.0 Hz, 3H), 7.52 (s, 1H), 7.44 (d, J = 8.0 Hz, 1H), 7.31 (t, J = 7.4 Hz, 2H), 7.06 (t, J= 7.4 Hz, 1H), 5.10 (dd, J= 13.2, 5.2 Hz, 1H), 4.44 (d, J= 17.2 Hz, 1H), 4.31 (d, J = 17.2 Hz, 1H), 3.25 (s, 2H), 3.16-2.98 (m, 2H), 2.95-2.69 (m, 1H), 2.66-2.57 (m, 1H), 2.49-2.40 (m, 2H), 2.38-2.31 (m, 2H), 2.00-1.90 (m, 1H), 1.87-1.77 (m, 4H).Example 46: 3-(5-(1-(3-fluoropropyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (I-181)

[0409]

[0410] To a stirred solution of I-155 (50 mg, 0.14 mmol) and Et 3 N (0.06 mL, 0.41 mmol) in DMF (2 mL) was added 1-fluoro-3-iodopropane (0.05 g, 0.27 mmol) and the resulting mixture was stirred at rt for 5 h. The reaction mixture was quenched with ice-cold water and extracted with EtOAc (2 x 50 mL). The combined organic extracts were washed with brine (25 mL) and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 10% MeOH in DCM. The pure fractions were evaporated under reduced pressure to afford I-181 as an off-white solid (15 mg, 0.04 mmol, 28% yield). MS [M+H] +< = 388.0. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.98 (s, 1H), 7.65 (d, J= 8.0 Hz, 1H), 7.49 (s, 1H), 7.40 (d, J = 8.0 Hz, 1H), 5.11 (dd, J = 13.2, 5.2 Hz, 1H), 4.56 (t, J = 5.8 Hz, 1H), 4.44 (t, J = 5.6 Hz, 1H), 4.43 (d, J= 17.2 Hz, 1H), 4.29 (d, J= 17.2 Hz, 1H), 2.99-2.88 (m, 3H), 2.67-2.49 (m, 4H), 2.44-2.33 (m, 4H), 2.00-1.97 (m, 2H), 1.78-1.71 (m, 4H).Example 47: 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoic acid (I-72)

[0411] Step 1. tert-butyl 4-methylbenzoate (47b)

[0412] A solution of 4-methylbenzoic acid 47a (5 g, 36.76 mmol) in thionyl chloride (15 mL) was heated to 70 °C for 3 h. Upon complete consumption of the starting material, thionyl chloride was evaporated under reduced pressure. The obtained material (3 g, crude) was taken into t-butanol (15 mL) and pyridine (3.5 mL, 35.44 mmol) was added at 0 °C and the resulting mixture was stirred at rt for 16 h. Upon complete consumption of the starting material, the reaction mixture was quenched with water and extracted with DCM (2 x 100 mL). The combined organic extracts were washed with brine (50 mL), dried over Na 2 SO 4 . and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 5% EtOAc in hexane. The pure fractions were collected and evaporated to afford compound 47b as pale brown liquid (3.3 g, 18.23 mmol, 50% yield). 1< H NMR (400 MHz, CDCl 3 ): δ 7.87 (d, J = 8.4 Hz, 2H), 7.20 (d, J = 8.4 Hz, 2H), 2.39 (s, 3H), 1.58 (s, 9H).Step 2. tert-butyl 4-(bromomethyl)benzoate (47c)

[0413] To a solution of 47b (3 g, 15.60 mmol) in carbon tetrachloride (30 mL) was added NBS (2.77 g, 15.60 mmol) followed by AIBN (260 mg, 1.56 mmol) and the resulting mixture was stirred at 60 °C for 8 h. Upon complete consumption of the starting material, the reaction mixture was cooled to rt, filtered through a small pad of Celite ®< filter aid, and washed with DCM. The combined organic extracts were dried over Na 2 SO 4 and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 2% EtOAc in hexane. The pure fractions were collected and evaporated under reduced pressure to afford 47c as a pale brown oil (2.2 g, 8.11 mmol, 52% yield). 1< H NMR (400 MHz, CDCl 3 ): δ 7.95 (d, J = 8.4 Hz, 2H), 7.43 (d, J= 8.4 Hz, 2H), 4.94 (s, 2H), 1.59 (s, 9H).Step 3. tert-butyl 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoate (I-182)

[0414] To as stirred solution of I-155 (500 mg, 1.37 mmol) and potassium carbonate (380 mg, 2.74 mmol) in DMF (5 mL) was added dropwise 47c (410 mg, 1.51 mmol) in DMF (2 mL) and the resulting mixture was stirred at rt for 16 h. Upon complete consumption of the starting materials, the reaction mixture was quenched with water and extracted with EtOAc (3 x 50 mL). The combined organic extracts were washed with brine (50 mL) and evaporated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 7% MeOH in DCM. The pure fractions were collected, evaporated, and dried to afford I-182 as pale brown solid (350 mg, 0.67 mmol, 49% yield). 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.98 (s, 1H), 7.87 (d, J= 10.4 Hz, 2H), 7.64 (d, J= 10.8 Hz, 1H), 7.49 (s, 1H), 7.44 (d, J= 10.4 Hz, 2H), 7.9 (d, J = 10.4 Hz, 1H) 5.10 (dd, J = 18.0, 6.4 Hz, 1H), 4.41 (d, J = 17.6 Hz, 1H), 4.28 (d, J = 17.6 Hz, 1H), 3.57 (s, 2H), 2.93-2.85 (m, 3H), 2.62-2.49 (m, 1H), 2.41-2.35 (m, 1H), 2.14-1.96 (m, 4H), 1.75-1.69 (m, 4H), 1.54 (s, 9H).Step 4. 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoic acid HCl salt (I-72)

[0415] To a solution of I-182 (300 mg, 0.57 mmol) in DCM (9 mL) was added 4M HCl in dioxane (5 mL) dropwise at 0 °C and the resulting mixture was stirred at rt for 30 h. Upon complete consumption of the starting material, the solvent was evaporated, triturated with diethyl ether, and the resulting solid was dried under reduced pressure to afford I-72 as an off-white solid (270 mg, 0.54 mmol, 94%, HCl salt). MS [M+H] +< = 461.85. 1< H NMR (400 MHz, DMSO-d 6 ): δ 13.2 (brs, 1H), 10.99 (s, 1H), 10.28 (brs, 1H), 8.05-8.01 (m, 2H), 7.79-7.71 (m, 3H), 7.45 (s, 1H), 7.38 (d, J = 8.4 Hz, 1H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H) 4.42-4.28 (m, 3H), 3.723.66 (m, 1H), 3.50-3.45 (m, 2H), 3.25-3.18 (m, 2H), 3.10-2.87 (m, 2H), 2.65-2.55 (m, 1H), 2.35-2.41 (m, 1H), 2.07-1.95 (m, 4H).Example 48: 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzamide (I-71)

[0416]

[0417] To a stirred solution of I-72 (250 mg, 0.50 mmol) and NH 4 Cl (40 mg, 0.75 mmol) in DMF (5 mL) was added DIPEA (0.27 mL, 1.5 mmol), followed by HATU (286 mg, 0.75 mmol) and the resulting mixture was stirred for 16 at rt. The reaction mixture was quenched with water and extracted with EtOAc (3 x 50 mL). The combined organic extracts were washed with brine (25 mL), dried over Na 2 SO 4 . and concentrated under reduced pressure. The crude material was purified by reverse phase HPLC to afford I-71 as an off-white solid (110 mg, 0.24 mmol, 47% yield). MS [M+H] +< = 460.8. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.99 (s, 1H), 7.95-7.85 (m, 3H), 7.65 (brs, 1H), 7.49-7.36 (m, 5H), 5.10 (dd, J = 13.2, 5.2 Hz, 1H), 4.43 (d, J = 17.2 Hz, 1H), 4.29 (d, J= 17.2 Hz, 1H), 3.15 (s, 2H), 2.95-2.86 (m, 3H), 2.67-2.57 (m, 2H), 2.40-2.36 (m, 2H), 2.05-1.98 (m, 2H), 1.80-1.65 (m, 4H).Example 49: 3-(5-(2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione hydrochloride (I-183)

[0418] Step 1. tert-butyl 2-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-3,6-dihydropyridine-1(2H)-carboxylate (49b)

[0419] To a solution of 49a (2 g, 9.38 mmol) in THF (10 mL) was added 1M LiHMDS (11.3 mL, 5.63 mmol) dropwise at -78 °C. After 1 h, 1,1,1-trifluoro-N-phenyl-N-((trifluoromethyl)sulfonyl) methane sulfonamide (3.68 g, 10.32 mmol) in THF (10 mL) was added dropwise, the temperature was gradually increased to rt and the resulting mixture was stirred for 16 h. The solvent was evaporated (below 40 °C) and the resulting residue was taken in diethyl ether (100 mL). The organic extract was then washed with 0.5M NaOH (2 x 50 mL) and brine (50 mL), dried over Na 2 SO 4 , and concentrated under reduced pressure to afford tert-butyl 2-methyl-4-(((trifluoromethyl)sulfonyl)oxy)-3,6-dihydropyridine-1(2H)-carboxylate as light yellow oil (2.4 g, crude). This crude material (2.4 g) was taken into dioxane (24 mL) and bis(pinacolato)diborane (970 mg, 3.82 mmol) was added followed by KOAc (625 mg, 6.37 mmol). The resulting mixture was degassed with argon for 10 min and PdCl 2 (dppf)•DCM (130 mg, 0.16 mmol) was then added in one portion. The reaction mixture was stirred at 110 °C for 16 h, then quenched with water, and extracted with EtOAc (3 x 100 mL). The combined organic extracts were washed with brine (50 mL), dried over Na 2 SO 4 , and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 10% EtOAc in hexane. The pure fractions were collected and evaporated under reduced pressure to afford 49b (1.85 g). This material was used in the next without further purification. Step 2. tert-butyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)-2-methyl-3,6-dihydropyridine-1(2H)-carboxylate (49c)

[0420] To a solution of 1c (800 mg, 2.48 mmol) and 49b (1.2 g, 3.72 mmol) in DMF (10 mL) was added K 2 CO 3 (685 mg, 4.96 mmol) followed by PdCl 2 (dppf)•DCM (101 mg, 0.12 mmol). The resulting mixture was degassed for 15 min and then stirred at 120 °C for 1 h in microwave. After complete consumption of the starting materials, the reaction mixture was quenched with water and extracted with EtOAc (2 x 50 mL). The combined organic extracts were washed with brine, dried over Na 2 SO 4 , and concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 80% EtOAc in hexane. The pure fractions were collected and evaporated under reduced pressure to afford 49c as a brown solid (620 mg, 1.41 mmol, 62% yield). MS [M+H] +< = 440.2.Step 3. tert-butyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)-2-methylpiperidine-1-carboxylate (49d)

[0421] To a solution of 49c (500 mg, 1.14 mmol) in DMF (5 mL), was added 10% Pd / C (100 mg) under an inert atmosphere and the resulting mixture was stirred at rt for 4 h under hydrogen atmosphere (balloon). After complete consumption of the starting material, the reaction mixture was passed through a pad of Celite ®< filter aid and washed with EtOAc. The filtrate was concentrated under reduced pressure. The crude material was purified by silica gel chromatography eluting with 10% MeOH in DCM to afford 49d as an off-white solid (400 mg, 0.90 mmol, 80% yield). MS [(M-C 4 H 8 )+H] +< = 386.1. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.97 (s, 1H), 7.65 (d, J = 8.0 Hz, 1H), 7.50 (s, 1H), 7.42 (d, J = 8.0 Hz, 1H), 5.08 (dd, J = 13.2, 5.2 Hz, 1H), 4.40 (d, J= 17.2 Hz, 1H), 4.30 (d, J= 17.2 Hz, 1H), 3.87-3.84 (m, 1H), 3.67-3.62 (m, 1H), 3.06-2.84 (m, 3H), 2.67-2.57 (m, 1H), 2.44-2.37 (m, 1H), 2.05-1.97 (m, 2H), 1.86-1.72 (m, 3H), 1.66-1.54 (m, 1H), 1.42 (s, 9H), 1.15 (d, J = 6.0Hz, 3H).Step 4. 3-(5-(2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione hydrochloride (I-183)

[0422] To a solution of 49d (100 mg, 0.22 mmol) in DCM (2 mL) was added 4M HCl in dioxane (1 mL) at 0 °C and the resulting mixture was stirred at rt for 4 h. After complete consumption of the starting material, the solvent was evaporated and the obtained material was triturated with diethyl ether and dried under reduced pressure to afford the hydrochloride salt of I-183 as an off-white solid (60 mg, 0.16 mmol, 70% yield). MS [M+H] +< = 342.0. 1< H NMR (400 MHz, DMSO-d 6 ): δ 10.98 (s, 1H), 8.89 (brs, 1H), 7.70 (d, J = 8.0 Hz, 1H), 7.50-7.37 (m, 2H), 5.11 (dd, J= 13.2, 5.2 Hz, 1H), 4.45 (d, J= 17.2 Hz, 1H), 4.31 (d, J = 17.2 Hz, 1H), 3.41-3.38 (m, 1H), 3.23-3.11 (m, 3H), 2.96-2.87 (m, 1H), 2.67-2.58 (m, 1H), 2.41-2.32 (m, 1H), 2.12-1.95 (m, 4H), 1.84-1.65 (m, 2H), 1.27 (d, J = 6.0 Hz, 3H).Example 50: trans-3-(5-(1 -((4-methoxycyclohexyl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione (1-303)

[0423] Step 1. trans-ethyl 4-methoxycyclohexane-1-carboxylate (50b)

[0424] To a stirred solution of 50a (2.0 g, 11.3 mmol) in THF (20 mL) was added NaH (700 mg, 17.4 mmol) in small portions at 0 °C. After stirring for 30 min, methyl iodide (1.45 mL, 23.2 mmol) was added and the resulting mixture was stirred at rt for 3 h. The reaction mixture was quenched with ice-cold water and extracted with EtOAc (2 x 100 mL). The combined organic extracts were washed with brine, dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure. The obtained crude material was purified by silica gel chromatography eluting with 20% EtOAc in hexane to afford 50b as a colorless oil (570 mg, 3.06 mmol, 26% yield). 1< H NMR (400 MHz, DMSO-d 6 ): δ 4.10 (q, J = 9.2 Hz, 2H), 3.34 (s, 3H), 3.16-3.08 (m, 1H), 2.26-2.11 (m, 1H), 2.07-1.99 (m, 4H), 1.61-1.39 (m, 2H), 1.28-1.19 (m, 2H), 1.24 (t, J = 9.2 Hz, 3H).Step 2. trans-4-methoxycyclohexane-1-carbaldehyde (50c)

[0425] To a solution of 50b (570 mg, 3.06 mmol) in DCM (10 mL) was added DIBAL-H (1 M, 3.67 mL, 3.67 mmol) dropwise at -78 °C and the resulting mixture was stirred for 4 h at -78 °C and then for 16 h at rt. The reaction mixture was diluted with DCM (20 mL) and quenched with saturated aq. Rochelle's salt. The organic layer was separated, washed with brine, dried over Na 2 SO 4 , filtered, and concentrated to dryness to afford the intermediate (4-methoxycyclohexyl)methanol (290 mg). This material was taken into DCM (10 mL), PCC (850 mg, 3.94 mmol) was added and the resulting mixture was stirred at rt for 2 h. The reaction mixture was then diluted with DCM (10 mL), filtered through a small pad of Celite ®< filter aid, and washed with DCM (10 mL). The combined filtrate was dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure to afford crude 50c (150 mg) as a pale brown oil which was used in the next step without further purification.Step 3. trans-3-(5-(1-((4-methoxycyclohexyl)methyl)piperidin-4-yl...

Claims

1. A compound of Formula (I'): or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, wherein: X1 is CR3; is optionally a double bond when X1 is CR3 and R3 is absent; each R1 is independently (C1-C6)alkyl, (C1-C6)haloalkyl, (C1-C6)hydroxyalkyl, or halogen, or two R1 together with the carbon atoms to which they are attached form a 5- or 6- membered heterocycloalkyl ring, or two R1, when on adjacent atoms, together with the atoms to which they are attached form a (C6-C10)aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S; R2 is (C1-C6)alkyl, -C(O)(C1-C6)alkyl, -C(O)(CH2)0-3(C6-C10)aryl, -C(O)O(CH2)0-3(C6-C10)aryl, (C6-C10)aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C3-C8)cycloalkyl, wherein the alkyl is optionally substituted with one or more R4; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one or more R5, or R1 and R2, when on adjacent atoms, together with the atoms to which they are attached form a 5- or 6-membered heterocycloalkyl ring; R3 is H or R3 is absent when is a double bond; each R4 is independently selected from -C(O)OR6, -C(O)NR6R6', -NR6C(O)R6', halogen, -OH, -NH2, CN, (C6-C10)aryl, 5- or 6-membered heteroaryl comprising 1 to 4 heteroatoms selected from O, N, and S, (C3-C8)cycloalkyl, and 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one or more R7; each R5 is independently selected from (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, CN, (C3-C7)cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C6-C10)aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R5, when on adjacent atoms, together with the atoms to which they are attached form a (C6-C10)aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10, or two R5, when on adjacent atoms, together with the atoms to which they are attached form a (C5-C7)cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one or more R10; R6 and R6', are each independently H, (C1-C6)alkyl, or (C6-C10)aryl; each R7 is independently selected from (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, -C(O)R8, -(CH2)0-3C(O)OR8, -C(O)NR8R9, -NR8C(O)R9, - NR8C(O)OR9, -S(O)pNR8R9, -S(O)pR12, (C1-C6)hydroxyalkyl, halogen, -OH, -O(CH2)1-3CN, -NH2, CN, -O(CH2)0-3(C6-C10)aryl, adamantyl, -O(CH2)0-3-5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C6-C10)aryl, monocyclic or bicyclic 5- to 10-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C3-C7)cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the alkyl is optionally substituted with one or more R11, and the aryl, heteroaryl, and heterocycloalkyl are optionally substituted with one or more substituents each independently selected from halogen, (C1-C6)alkyl, (C1-C6)haloalkyl, and (C1-C6)alkoxy, or two R7 together with the carbon atom to which they are attached form a =(O), or two R7, when on adjacent atoms, together with the atoms to which they are attached form a (C6-C10)aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10, or two R7 together with the atoms to which they are attached form a (C5-C7) cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10; R8 and R9 are each independently H or (C1-C6)alkyl; each R10 is independently selected from (C1-C6)alkyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, and CN, or two R10 together with the carbon atom to which they are attached form a =(O); each R11 is independently selected from CN, (C1-C6)alkoxy, (C6-C10)aryl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl and heterocycloalkyl are optionally substituted with one or more substituents each independently selected from (C1-C6)alkyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, and CN; R12 is (C1-C6)alkyl, (C1-C6)haloalkyl, (C6-C10)aryl, or 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S; Rx is H or D; p is 0, 1, or 2; n is 0, 1, or 2; n1 is 1 or 2, wherein n + n1 ≤ 3; and q is 0, 1, 2, 3, or 4.

2. The compound of claim 1, having a Formula (I): or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, wherein: X1 is CR3; is optionally a double bond when X1 is CR3 and R3 is absent; each R1 is independently (C1-C6)alkyl, (C1-C6)haloalkyl, (C1-C6)hydroxyalkyl, or halogen; R2 is (C1-C6)alkyl, (C6-C10)aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or (C3-C8)cycloalkyl, wherein the alkyl is optionally substituted with one or more R4; and the aryl, heteroaryl, and cycloalkyl are optionally substituted with one or more R5; R3 is H or R3 is absent when ------ is a double bond; each R4 is independently selected from -C(O)OR6, -C(O)NR6R6, -NR6C(O)R6', (C6-C10)aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C3-C8)cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl groups are optionally substituted with one or more R7; each R5 is independently selected from (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, CN, (C3-C7)cycloalkyl, 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, (C6-C10)aryl, and 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R5, when on adjacent atoms, together with the atoms to which they are attached form a (C6-C10)aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10, or two R5, when on adjacent atoms, together with the atoms to which they are attached form a (C5-C7)cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S optionally substituted with one or more R10; R6 and R6, are each independently H or (C1-C6)alkyl; each R7 is independently selected from (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, -C(O)R8, -C(O)OR8, -C(O)NR8R9, -NR8C(O)R9, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, CN, (C6-C10)aryl, 5- or 6-membered heteroaryl comprising 1 to 3 heteroatoms selected from O, N, and S, (C3-C7)cycloalkyl, and 5- to 7-membered heterocycloalkyl comprising 1 to 3 heteroatoms selected from O, N, and S, or two R7, when on adjacent atoms, together with the atoms to which they are attached form a (C6-C10)aryl ring or a 5- or 6-membered heteroaryl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10, or two R7, when on adjacent atoms, together with the atoms to which they are attached form a (C5-C7)cycloalkyl ring or a 5- to 7-membered heterocycloalkyl ring comprising 1 to 3 heteroatoms selected from O, N, and S, optionally substituted with one or more R10; R8 and R9 are each independently H or (C1-C6)alkyl; each R10 is independently selected from (C1-C6)alkyl, (C1-C6)alkoxy, (C1-C6)haloalkyl, (C1-C6)haloalkoxy, (C1-C6)hydroxyalkyl, halogen, -OH, -NH2, and CN; Rx is H or D; n is 1 or 2; and q is 0, 1, 2, 3, or 4, preferably q is 0, 1, or 2.

3. The compound according to claim 1 or 2, wherein n is 1.

4. The compound according to claim 1 or 2, wherein n is 2.

5. The compound according to any one of the preceding claims, wherein X1 is CH.

6. The compound according to any one of the preceding claims, wherein Rx is H.

7. The compound according to claim 1, having a Formula (Ia), Formula (Ib), Formula (Ic), or Formula (Id):

8. The compound according to any one of the preceding claims, wherein R2 is (C6-C10)aryl or (C3-C8)cycloalkyl, wherein the aryl and cycloalkyl are optionally substituted with one to three R5, preferably R2 is (C6-C10)aryl or (C3-C8)cycloalkyl.

9. The compound according to any one of claims 1-7, wherein R2 is (C1-C6)alkyl optionally substituted with one to three R4.

10. The compound according to claim 1 selected from: 3-(5-(1-ethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-propylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(cyclopropylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-isobutylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(cyclobutylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(oxazol-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(thiazol-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(cyclopentylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((5-chlorothiophen-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2-chlorothiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(cyclohexylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2-(pyrrolidin-1-yl)ethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((tetrahydro-2H-pyran-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-phenethylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2-(piperidin-1-yl)ethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((3,5-dimethylisoxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1,3-dimethyl-1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((6-methylpyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-morpholinopropyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,6-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,6-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-dibromobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-chloro-5-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,5-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,5-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzonitrile; 3-(5-(1-(4-(hydroxymethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,4-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-chloro-2-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-chloro-4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzonitrile; 3-(5-(1-(2,3-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzonitrile; 3-(5-(1-(4-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,5-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,4-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,4-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-indazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-benzo[d]imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-isopropylbenzyl)piperidin-4-yl)-1-oxoisoindolin -2-yl)piperidine-2,6-dione; methyl 5-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)furan-2-carboxylate; 3-(5-(1-(naphthalen-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(quinolin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(naphthalen-1-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-methyl-1H-benzo[d]imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(4-(trifluoromethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(1H-pyrrol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(1H-1,2,4-triazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(3-(trifluoromethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2-(trifluoromethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyridin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyridin-3-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyrimidin-5-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(fluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,4-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)pyrimidine-5-carbonitrile; 3-(5-(1-(4-ethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2-methoxypyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-fluoro-4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(difluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzamide; 4-((4-(2-(2,6-dioxopiperidm-3-yl)-1-oxoisoindolin-5-yl)pipendin-1-yl)methyl)benzoic acid; 3-(5-(1-(3-(difluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoic acid; 3-(1-oxo-5-(1-(4-propylbenzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(4-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(difluoromethoxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((5-(trifluoromethyl)pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-(difluoromethoxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-(difluoromethoxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-cyclobutylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2,3-dihydrobenzo[b][1,4]dioxin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(tert-butyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-isobutylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; N-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetamide; 3-(5-(1-((2,2-difluorobenzo[d][1,3]dioxol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(4-(tert-pentyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-([1,1'-biphenyl]-4-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3 -(5-(1-(4-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(1H-imidazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-(1H-pyrazol-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-cyclohexylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyrimidin-2-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-bromobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-chlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione 3-(5-(1-(4-chloro-3-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-chloro-4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,4-difluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-methoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(benzo[c][1,2,5]oxadiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-cyclopropylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1,3-dihydroisobenzofuran-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2-(trifluoromethyl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-isopropoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(4-(thiophen-3-yl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-cyclopentylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(4-(pyrrolidin-1-yl)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-fluorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,4-dichlorobenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(quinolin-8-y1methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-methyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-methyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-pyrrol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-imidazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-ethyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3 -(5 -(1-((2-aminopyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((6-aminopyridin-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((5-amino-1-methyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((6-methylimidazo[2,1-b]thiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(imidazo[1,2-a]pyrazin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-([1,2,4]triazolo[1,5-a]pyridin-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(pyrazolo[1,5-a]pyridin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1,4-dimethyl-1H-imidazol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(benzo[d]thiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3 -(1-oxo-5 -(1-(pyrazolo [1,5-a]pyrimidin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3 -(5 -(1-(imidazo [1,2-a]pyrimidin-3-ylmethyl)piperidin-4-yl)-1-oxoisoindofin-2-yl)piperidine-2,6-dione; 3-(5-(1-(imidazo[1,2-a]pyrimidin-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-cyclobutyl-1H-1,2,3-triazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((4,5,6,7-tetrahydropyrazolo[1,5-a]pyridin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-indol-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-indazol-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-pyrrolo[2,3 -b]pyridin-3 -yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzamide; 3-(5-(1-((1H-pyrrolo[2,3-b]pyridin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((3,4-dihydro-2H-benzo[b][1,4]thiazin-6-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((2-(pyrrolidin-1-yl)pyrimidin-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2-(tert-butyl)thiazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((2-(thiophen-2-yl)thiazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((5-cyclopropyl-1H-pyrazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2-morpholinopyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((3-phenyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((6-methyl-1H-indol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; methyl 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-1H-pyrrole-2-carboxylate; 3-(1-oxo-5-(1-((3-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((5-(pyridin-2-yl)-1H-pyrazol-3-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-difluoro-4-hydroxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-((2S)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin -2-yl)piperidine-2,6-dione; 3-(5-((2R)-1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzyl-2-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-((R)-azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-((S)-azepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((1,2,3,4-tetrahydronaphthalen-1-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; methyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate; 3-(1-oxo-5-(1-phenylpiperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3-methylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,6-dimethylbenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((5,6,7,8-tetrahydronaphthalen-2-yl)methyl)piperidin -4-yl)isoindolin-2-yl)piperidine-2,6-dione; ethyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate; tert-butyl 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetate; 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)acetic acid; 3-(1-oxo-5-(1-(3,3,3-trifluoropropyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 2-(4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)-N-phenylacetamide; 3-(5-(1-(3-fluoropropyl)piperidin-4-yl)-1-oxolsoindolin-2-yl)piperidine-2,6-dione; tert-butyl 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)benzoate; 3-(5-(1-benzyl-3,3-dimethylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzyl-3-methylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzyl-2,5-dihydro-1H-pyrrol-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzyl-2-oxopiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzyl-1,2,3,4-tetrahydroquinolin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-benzyl-1H-tetrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((5-phenyl-1,3,4-oxadiazol-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(benzo[d]thiazol-2-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((3-(pyridin-2-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((R)-2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-methyl-1H-indazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1,2,4-oxadiazol-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-hydroxy-3-((4-methylpiperazin-1-yl)methyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetonitrile; 3-(5-(1-((7-hydroxy-2-methylpyrazolo[1,5-a]pyrimidin-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,2-difluoro-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((3 -fluorobicyclo[1.1.1]pentan-1-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2-fluoro-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((4-oxo-3,4-dihydrothieno [3,2-d]pyrimidin-2-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(quinolin-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(3,5-bis(trifluoromethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 6-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)picolinonitrile; 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenoxy)acetonitrile; 3-(5-(1-((1H-indazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(2,2-difluoroethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((7-methyl-4-oxo-4H-pyrido[1,2-a]pyrimidin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; benzyl 4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidine-1-carboxylate; 3-(1-oxo-5-(1-(2-phenylacetyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2,2,2-trifluoro-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(5-methylbenzo[d]thiazol-2-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(isoquinolin-1-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(4-methoxypiperidin-1-yl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(isopropylthio)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((S)-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 2-(4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)phenyl)acetic acid; 3-(5-(1-((7-fluoroquinolin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((2-amino-4-(trifluoromethyl)thiazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-1,2,4-oxadiazole-5-carboxamide; 3-(5-(1-(3-(morpholinosulfonyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 4-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-N,N-dimethylbenzenesulfonamide; 3-(1-oxo-5-(1-(thiazol-4-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(quinoxalin-6-ylmethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(tert-butyl)benzoyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-((4-fluorobenzyl)oxy)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((3-methylisoxazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(isoxazol-3-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((R)-1-phenylethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-(methoxymethyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((S)-2-hydroxy-1-phenylethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(phenylsulfonyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((5-methyl-3-phenylisoxazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-((difluoromethyl)sulfonyl)benzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-(2,2,2-trifluoroethyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; methyl 2-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)oxazole-4-carboxylate; 3-(1-oxo-5-(1-(4-(pyridin-2-ylmethoxy)benzyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-acetylpiperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzylpyrrolidin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (R)-3-(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (S)-3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-methyl-2,3,6,7-tetrahydro-1H-azepin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(8-benzyl-8-azabicyclo[3.2.1]octan-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(8-azabicyclo[3.2.1]octan-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-acetyl-1,2,5,6-tetrahydropyridin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (R)-3-(5-((R)-1-acetylpyrrolidin-3-yl)-1-oxoisoindofin-2-yl)piperidine-2,6-dione; 3-(5-(1-acetyl-1,2,3,6-tetrahydropyridin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(octahydroindolizin-7-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (R)-3-(5-((S)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-acetyl-2,5-dihydro-1H-pyrrol-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-methylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (S)-3-(5-((R)-1-benzylazepan-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; (S)-3-(5-((R)-1-acetylpyrrolidin-3-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((6-isopropoxypyridin-3-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((1-phenyl-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(4-ethoxybenzyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((1-phenyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-isopropyl-1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-(isothiazol-5-ylmethyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-isopropyl-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1H-pyrazol-5-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((5-isopropoxypyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5 -(1-((1-(pyridin-3-yl)-1H-pyrazol-5-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 3-(1-oxo-5-(1-((1-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; 5-((4-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-5-yl)piperidin-1-yl)methyl)-2-fluorobenzonitrile; 3-(5-(1-((5-fluoropyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; 3-(5-(1-((1-ethyl-3-(pyridin-3-yl)-1H-pyrazol-4-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; trans-3-(1-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; cis-3-(1-oxo-5-(1-((4-(trifluoromethyl)cyclohexyl)methyl)piperidin-4-yl)isoindolin-2-yl)piperidine-2,6-dione; trans-3-(5-(1-((4-methoxycyclohexyl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; and 3-(5-(1-((6-methoxypyridin-2-yl)methyl)piperidin-4-yl)-1-oxoisoindolin-2-yl)piperidine-2,6-dione; or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof.

11. The compound according to claim 1, wherein the compound is: or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof.

12. A pharmaceutical composition comprising a compound according to any one of the claims 1-11, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, and a pharmaceutically acceptable carrier or excipient, optionally further comprising at least one additional pharmaceutical agent.

13. A compound according to any one of the claims 1-11, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use as a medicament.

14. A compound according to any one of the claims 1-11, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, or the pharmaceutical composition of claim 12 for use in the treatment of cancer.

15. The compound or the pharmaceutical composition for use according to claim 14, wherein the cancer is selected from a cancer for which the immune response is deficient or an immunogenic cancer, non-small cell lung cancer (NSCLC), melanoma, triple-negative breast cancer (TNBC), nasopharyngeal cancer (NPC), microsatellite stable colorectal cancer (mssCRC), thymoma, carcinoid, acute myelogenous leukemia, and gastrointestinal stromal tumor (GIST).

16. A compound according to any one of claims 1-11, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, for use in the treatment of an IKZF2-dependent disease or disorder.

17. A pharmaceutical combination comprising a compound according to any one of claims 1-11, or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, and one or more additional therapeutic agent(s).