METHOD FOR PRODUCING HAPLOID / DOUBLE HAPLOID SUNFLOWER PLANTS IN A DOUBLE-LAYER MICROSPORE CULTURE SYSTEM

Abstract

The present invention relates to a method for producing haploid, polyhaploid, and / or double haploid embryos, calluses, seeds, and / or plants of the species Helianthus annuus from isolated microspore cultures, more specifically to a method comprising contacting the isolated microspores with a histone deacetylase inhibitor (HDACi) and / or a histone methyltransferase inhibitor (HMTi) and a complex protein composition, wherein the culture is carried out in a double-layer system. The present invention also provides a kit for producing such a haploid, polyhaploid, and / or double haploid embryo, callus, seed, and / or plant. Finally, the present invention also relates to a population of haploid, polyhaploid, and / or double haploid plants of Helianthus annuus directly derived from at least one apical or lateral sunflower head.