Bispecific anti-VEGF / anti-ANG-2 antibody

Abstract

A method for the reduction of the viscosity of an antibody wherein the antibody comprises a constant heavy chain region of human IgG1 subclass wherein the method comprises the modification of the antibody constant heavy chain region of human IgG1 subclass with the mutations 1253A, H310A, and H435A, numbering according to EU Index of Kabat; and wherein the antibody is a bispecific antibody comprising a first antigen-binding site that specifically binds to human VEGF and a second antigen-binding site that specifically binds to human ANG-2, wherein said first antigen-binding site specifically binding to VEGE comprises in the heavy chain variable domain a CDR3H region of SEQ ID NO: 1, a CDR2H region of SEQ ID NO: 2, and a CDR1H region of SEQ ID NO:3, and in the light chain variable domain a CDR3L region of SEQ ID NO: 4, a CDR2L region of SEQ ID NO:5, and a CDR1L region of SEQ ID NO:6; and said second antigen-binding site specifically binding to ANG-2 comprises in the heavy chain variable domain a CDR3H region of SEQ ID NO: 9, a CDR2H region of, SEQ ID NO: 10, and a CDR1H region of SEQ ID NO: 11, and in the light chain variable domain a CDR3L region of SEQ ID NO: 12, a CDR2L region of SEQ ID NO: 13, and a CDR1L region of SEQ ID NO: 14.