Method of manufacturing bispecific antibodies, bispecific antibodies and therapeutic use of such antibodies

Abstract

The present invention relates to a bivalent bispecific monoclonal antibody (bbmAb) suitable for co-expression in a common host cell, wherein the antibody comprises a first variable heavy chain of wild type (VH1) that binds specifically to a first target, and a first constant heavy chain (CH1) with a hetero-dimerization modification and a second part which is an immunoglobulin with a second variable light chain of kappa wild type (VL2) and a second variable heavy chain of wild type (VH2), that binds specifically to a second target, different from the first target, and a second constant heavy chain (CH2) with a hetero-dimerization modification which is complementary to the hetero-dimerization modification of the first constant heavy chain. The present invention further relates to a method of manufacturing such antibody by co-expressing modified Fc-mutated derivatives of two different monoclonal antibodies in mammalian cell lines. It further relates to a pharmaceutical composition comprising the claimed antibody for use as a medicament.