A non contact real time micro polymerase chain reaction system and method thereof

Abstract

Tfie present disclosure provides a non con-’ tact . real time micro Polymerase Chain Readion [PCR] system comprises; a chip having - a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising associated LED driver and photo detector amplifier placed above the chip to detect the fluorescence, an induction heater mounted around the chip and is inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein said infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the Induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.