Method for detecting gamma butyrolactone, 4-hydroxybutyric acid and 4-hydroxybutyric acid salts in a solution

Abstract

The subject of the application is a method for detecting the presence of gamma butyrolactone, 4-hydroxybutyric acid, and 4-hydroxybutyric acid salts in a solution, in which a selected metal-organic network is used, into which a dye is introduced: acriflavine (AF) or 3,6-diaminoacridine (proflavine, PF) or 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-imino chloride (pyronin Y, PYY). The liquid sample to be tested is spotted onto the prepared material, ethanol and an auxiliary solvent are added, and the sample is examined using a spectrofluorometer, setting the parameters: emission measurement range 470 to 700 nm excitation wavelength in the range from 420 to 470 nm. The shift of the fluorescence spectrum maximum towards shorter wavelengths compared to the reference sample indicates that in the tested solution gamma butyrolactone, 4-hydroxybutyric acid or 4-hydroxybutyric acid salts have a concentration in the range from 16 µM to 236 µM.