Polydopamine modified with poly(N-isopropylacrylamide) nanocarrier, method for producing the same, and use as a coating for porous nanoparticles in the process of externally triggered release of hydrophilic drugs

Abstract

The subject of the application is a polydopamine nanocarrier modified with poly(N-isopropylacrylamide), a method for its production and its use as a coating for porous nanoparticles in the process of releasing hydrophilic drugs initiated by external factors. The polydopamine nanocarrier modified with poly(N-isopropylacrylamide) of the formula: A-AL-B wherein: A - porous silica, AL - porous silica with an anticancer drug wherein: ALB porous silica with an anticancer drug coated with a layer of polydopamine modified with poly(N-isopropylacrylamide). The method of producing the nanocarrier consists in the following: in the first step, porous silica (mSiO2) is obtained by reacting 1 - 10 mM hexadecyltrimethylammonium bromide (CTAB), preferably 3 mM in deionized water, with continuous stirring for 10 - 120 minutes, preferably 30 minutes, at a temperature of 40°C - 60°C, preferably 40°C, then alcohol, preferably ethyl alcohol, and ammonium hydroxide solution are added to the solution,then the whole is stirred for 1 - 10 minutes, preferably 5 minutes, then 4.7 mL of tetraethyl orthosilicate (TEOS) is added and the contents are stirred for 24 - 48 hours at 40°C - 100°C, preferably for 48 hours at 60°C, then the solution is cooled to room temperature and washed with ethanol on a 0.22 µm mSiO2 filter, dried at 60°C - 100°C for 12 - 48h, preferably 24 hours, then CTAB is removed by placing 2 g of mSiO2 in a tube furnace at 600°C for 2 - 12 hours, preferably 4 hours in an air atmosphere, in the second step 5 - 20 mg of a hydrophilic anticancer drug, preferably 10 mg is dissolved in a 10 mM PBS buffer solution, pH 7.4 at room temperature using ultrasound for 5 - 20 minutes, preferably for 10 minutes, then mSiO2 in the amount of 1 - 10 mg, preferably 5 mg is dispersed ultrasonically in a 10 mM PBS buffer solution, pH 7.4 at room temperature,and the resulting mixture is magnetically stirred at 25°C for 12 - 24 hours, preferably 24 hours, and in order to remove the physically adsorbed drug, the obtained AL particles are collected by centrifugation and gently washed with Milli-Q water. In the third step, 15 mg of AL is ultrasonically dispersed in 10 mM Tris buffer with pH 8.5 for 5 - 20 minutes, preferably 10 minutes, then 1 - 100 mg, preferably 10 mg of dopamine hydrochloride and 1 mg - 100 mg, preferably 10 mg of N-isopropylacrylamide are added to the dispersion of silica nanoparticles with the anticancer drug, then the suspension is continuously stirred for 12 - 24 hours, preferably 24 hours at 25°C, then the mixture is centrifuged, washed with Tris buffer with pH 8.5 and Milli-Q water to obtain a nanocarrier.