Method of stabilizing lactoferrin in the spray drying process

Abstract

The subject of the application is a method for obtaining lactoferrin powder stabilized with iron (III) which consists in mixing a lactoferrin solution with a concentration of 2.5 to 100 mg / mL in a buffer solution containing 5 to 20 mM trisodium citrate at a pH of 6.5 to 8.5, containing 0.01% to 0.1% sodium chloride and iron (III) in the form of iron (III) lactate or iron (III) chloride or iron (III) sulfate or iron (III) nitrate and a lactoferrin:Fe(III) molar ratio of 1:1 to 1:12 in an atmosphere of air or an inert gas, preferably nitrogen or argon, most preferably for 10 to 120 min.at a temperature of 20°C to 30°C, and then the obtained solution is dried by spraying in a spray dryer equipped with a double-stream atomizer or a rotating spray disc at a concentrate flow of 0.5 l / g to 10 l / g, at an inlet temperature of 160°C to 220°C and an outlet temperature of 70°C to 100°C, a drying air flow in the range of 20 — 300 m3 / h and a particle residence time in the drying chamber of no longer than 15 seconds.