Bioprocessing systems and methods

Claims

1. Bioprocessing system including: a first fluid assembly having a first fluid assembly line connected to a first nozzle of a first bioreactor vessel via a first line of the first bioreactor vessel, wherein the first line of the first bioreactor vessel comprises a first line valve for providing a selective fluid connection between the first fluid assembly and the first nozzle of the first bioreactor vessel; a second fluid assembly having a second fluid assembly line connected to a second nozzle of the first bioreactor vessel via a second line of the first bioreactor vessel, wherein the second line of the first bioreactor vessel comprises a second line valve for providing a selective fluid connection between the second fluid assembly and the second nozzle of the first bioreactor vessel, and a connecting line providing a fluid connection between the first fluid assembly and the second fluid assembly and a fluid connection between the second line of the first bioreactor vessel and the first line of the first bioreactor vessel.

2. The bioprocessing system according to paragraph 1, in which: the connecting line is connected to the second line of the first bioreactor vessel and the first line and the first fluid assembly; and wherein the connecting line comprises a connecting line valve for providing a selective fluid connection between at least the second line of the first bioreactor vessel and the second fluid assembly.

3. The bioprocessing system according to paragraph 1, in which: the first fluid assembly comprises a plurality of tail tube sections, wherein each of the tail tube sections of the first fluid assembly is configured to connect to one of the plurality of first reservoirs; and the second assembly comprises a plurality of tail sections of tubes, where each of the tail sections of tubes of the second fluid assembly is intended to be connected to one of the plurality of second reservoirs.

4. The bioprocessing system according to paragraph 3, in which: each of the tail portions of the first fluid tube assembly comprises a tail portion valve for regulating the flow of fluid into or out of a corresponding one of the plurality of first reservoirs; and each of the tail sections of the second fluid assembly tubes includes a tail section tube valve for regulating the flow of fluid into or out of the corresponding one of the plurality of second reservoirs.

5. The bioprocessing system according to paragraph 1, further comprising: a sampling assembly connected to at least one of the first fluid assembly, the second fluid assembly, the connecting line, and / or the flow path portion between the second line valve and the first line valve, wherein the sampling assembly comprises a sampling line that provides fluid communication between the sampling assembly and the second nozzle of the first bioreactor vessel.

6. The bioprocessing system according to paragraph 5, in which: The sampling assembly is connected to the connecting line between the second line valve and the first line valve.

7. The bioprocessing system according to paragraph 5, in which: the sampling assembly comprises at least one configuration of tail sections of tubes for connection to the sampling end.

8. The bioprocessing system according to paragraph 1, further comprising: a first pump capable of providing a fluid flow in two directions, located along the line of the first fluid assembly and designed to initiate a fluid flow between the first fluid assembly and the first bioreactor vessel, and between the first fluid assembly and the second fluid assembly.

9. The bioprocessing system according to paragraph 8, further comprising: a second pump capable of providing fluid flow in two directions, designed to initiate fluid flow between the second fluid assembly and the first bioreactor vessel.

10. The bioprocessing system according to paragraph 9, in which: a second pump capable of providing a flow of fluid in two directions is designed to initiate a flow of fluid between the first bioreactor vessel and the second bioreactor vessel.

11. The bioprocessing system according to paragraph 9, in which: The first pump and the second pump are extended dynamic range pumps.

12. The bioprocessing system according to paragraph 2, further comprising: a filtration line that is in fluid communication with the connecting line and the first line of the first bioreactor vessel; and a filter located along the filtration line.

13. The bioprocessing system according to paragraph 12, in which: The filter line surrounds the connecting line valve so that fluid flow through the connecting line can be directed through the filter line.

14. The bioprocessing system according to paragraph 13, further comprising: a waste-permeate line providing a fluid connection between the filter and a waste tank connected to the second fluid assembly for transferring waste removed from the fluid to the waste tank.

15. The bioprocessing system according to paragraph 14, further comprising: a waste-permeate pump designed to pump waste removed by the filter into a waste tank.

16. The bioprocessing system according to paragraph 13, in which: The filter is an elongated hollow fiber filter and a membrane filter in the form of a flat sheet.

17. The bioprocessing system according to paragraph 5, further comprising: a sterile air source line connected to at least one of the first fluid assembly, the second fluid assembly, the connecting line and / or the flow path portion between the second line valve and the first line valve, wherein the sterile air source line includes a sterile air source line valve for selectively regulating the flow of sterile air from the sterile air source to at least one of the first fluid assembly, the second fluid assembly and / or the connecting line.

18. The bioprocessing system according to paragraph 1, in which: the first fluid assembly line of the first fluid assembly is further connected to the first nozzle of the second bioreactor vessel through the first line of the second bioreactor vessel, wherein the first line of the second bioreactor vessel comprises a first line valve for providing a selective fluid connection between the first fluid assembly and the first nozzle of the second bioreactor vessel; and wherein the second fluid assembly line of the second fluid assembly is connected to the second nozzle of the second bioreactor vessel through the second line of the second bioreactor vessel, wherein the second line of the second bioreactor vessel comprises a valve of the second bioreactor vessel for providing a selective fluid connection between the second fluid assembly and the second nozzle of the second bioreactor vessel.

19. The bioprocessing system according to paragraph 18, in which: the connecting line provides a fluid connection between the second line of the second bioreactor vessel and the first line of the second bioreactor vessel.

20. The bioprocessing system according to paragraph 19, in which: the connecting line provides a fluid connection between the second line of the second bioreactor vessel and the second fluid assembly.