A recombinant plasmid expressing the cloned Vibro cholerae DNase gene and the Escherichia coli strain JM109 pDN-33, a superproducer of Vibro cholerae DNase.
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Patent Information
- Application Number
- RU2023118187
- Authority / Receiving Office
- RU · RU
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2023-07-10
- Publication Date
- 2026-09-04
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Claims
1. Recombinant plasmid pDN-33 expressing the cloned gene of cholerae vibrio DNase in E.coli strains, cloning of the V.cholerae DNase gene in the commercial plasmid vector pQE30, under the control of the T5 promoter.
2. The E. coli strain JM109 pDN-33, deposited in the State Collection of Pathogenic Bacteria of the Russian Anti-Plague Research Institute “Microbe” of Rospotrebnadzor under number KM 2116, is a product obtained by transforming the recombinant plasmid pDN-33 according to item 1 into the Escherichia coli strain JM109, and is a superproducer of V. cholerae DNase.