A set of synthetic oligonucleotides and analog DNA, genetic testing methods, and genetic testing kits for detecting highly pathogenic PRRS virus (HP-PRRS virus) strains in pigs.

Abstract

------15 / 06 / 2561------(OCR) Page 1 of 1 Summary of the invention. A set of oligonucleotides and an analog DNA that... Designed and synthesized through a chemical process, it is specific for detecting genetic material. The highly pathogenic PRRS virus (HP-PRRS virus) in pigs, at the following gene location. NSP2 (Nsp 2) was used to develop methods for detecting the virus, including in terms of augmentation. Quantifying the target genetic material using the single-plane loop DNA synthesis technique. Mediated isothermal amplification (LAMP) and genetic signal monitoring. The principle of nucleic acid hybridization, combined with labeling. Fluorescent molecules are a highly accurate and specific method for detecting viral genetic material. Furthermore, it exhibits high reaction sensitivity and effectively overcomes the limitation of false positives. (Method) The developed kit can be applied to detect pathogenic PRRS virus strains that are present. In the form of an in vitro chemical analysis operating system, on a zipper, or on a membrane or casing. A paper that is efficient, fast, low-cost, and convenient to use in the field. ------------ Revised September 5, 2017 A set of oligonucleotides and an analog DNA that... Designed and synthesized through a chemical process, it is specific for detecting genetic material. The PRRS virus is a variant that causes severe disease in pigs (HP-PRRS virus) at a specific gene location. NST2 (Nsp 2) was used to develop methods for detecting the virus, including in terms of augmentation. Quantifying the target genetic material using the single-plane loop DNA synthesis technique. Mediated isothermal amplification (LAMP) and genetic signal monitoring. The principle of nucleic acid hybridization, combined with labeling. Molecular fluorescence is a highly accurate and specific method for detecting viral genetic material. Furthermore, it exhibits high reaction sensitivity and effectively overcomes the limitation of false positives. (Method) The developed kit can be applied to detect pathogenic PRRS virus strains that are present. In the form of in vitro, on-chip, or membrane-based chemical analysis systems. A paper that is efficient, fast, low-cost, and convenient to use in the field. ---------- A set of oligonucleotides and an analog DNA that... Designed and synthesized through a chemical process, it is specific for detecting genetic material. The PRRS virus is a variant that causes severe disease in pigs (HP-PRRS virus) at a specific gene location. NDP2 (Non-Stereo-Traffic Acid-2) was used to develop methods for detecting the virus, including the enhancement of its capabilities. Quantifying the target genetic material using the single-plane loop DNA synthesis technique. Mediated isothermal amplification (LAMP) and genetic signal monitoring. The principle of nucleic acid hybridization, combined with the attachment of... Molecular fluorescence is a highly accurate and specific method for detecting viral genetic material. Furthermore, it exhibits high reaction sensitivity and effectively overcomes the limitation of false positives. (Method) The developed kit can be applied to detect pathogenic PRRS virus strains that are present. In the form of in vitro, on-chip, or membrane-based chemical analysis systems. A paper that is efficient, fast, low-cost, and convenient to use in the field.