Recipe for feeding Ambrosia beetles and production methods.

Abstract

OCR 09WP 05 / 09 / 2568 The formula for feeding Ambrosia beetles and the production method include 500 milligrams of salt. Yeast extract 3,000 mg, casein 5,000 mg, fine starch powder. Powder) 3,000 milligrams, sucrose 10,000 milligrams, agar 20,000 milligrams, and... 70,000 milligrams of fine pine sawdust are mixed in 600 milliliters of sterilized distilled water. Then... Bring the ingredients to a boil for 20-25 minutes to allow them to meld. Pour the mixture into bottles, then sterilize them in a steam sterilizer. Vapor pressure at 121 degrees Celsius, 15 pounds per square inch pressure for 15 minutes, then rest until... The mixture is heated to 60 degrees Celsius. Add 3 milligrams of the antibiotic streptomycin and stir until the mixture is well combined. The culture medium is then divided into 150 x 16 mm test tubes that have been sterilized in an autoclave. (Autoclave) Close the tube with a cotton stopper and let it stand for 24 hours. Then, take the sterilized Ambrosia moth sample. On the surface, immerse the sample moth in 95 percent alcohol for 30 seconds and then move it. Moths are placed on sterilized tissue paper, allowing them to crawl on the paper for 1-2 minutes before being transferred to test tubes. This process... The resulting moths are clean and capable of self-development, laying eggs and progressing to other moth stages.

Claims

OCR 09WP 22 / 12 / 2568 1. The formula for feeding Ambrosia beetles consists of: 500 milligrams of salt Yeast extract 3,000 mg Casein 5,000 milligrams 3,000 milligrams of fine starch powder. 10,000 milligrams of sucrose. 20,000 milligrams of agar 70,000 milligrams of fine pine sawdust. 3 milligrams of the antibiotic streptomycin. 600 ml of sterilized distilled water.

2. A feed formula for Ambrosia beetles, as per claim 1, which may include glucose. It can be used as a substitute for sucrose.

3. A formula for feeding Ambrosia beetles according to claim 1, which can be supplemented with agar. (agar) 21,000 - 30,000 milligrams 4. A feed formula for Ambrosia beetles under claim 1 that may include mango sawdust. And durian sawdust can be used as a substitute for finely ground pine sawdust.

5. The method for producing feed for Ambrosia beetles using the formula described in Claim 1 involves the following steps: A. Measure 600 milliliters of sterilized distilled water and pour it into the pot. Then add the culture medium components. These include 500 milligrams of salt, 3,000 milligrams of yeast extract, and 5,000 milligrams of casein. 3,000 mg of fine starch powder, 10,000 mg of sucrose, agar. Add 20,000 milligrams of finely ground pine sawdust and 70,000 milligrams of crushed pine sawdust, then stir until the mixture is well combined. B. Bring the mixture to a boil over medium heat for 20-25 minutes, then let it simmer gently for 1-2 minutes. During cooking, stir the food constantly to prevent it from sticking to the bottom of the pot. C. When the time is up, pour the prepared food into bottles and then sterilize them using a steam sterilizer. At a temperature of 121 degrees Celsius and a pressure of 15 pounds per square inch for 15 minutes. d. Let the mixture rest until it reaches 60 degrees Celsius. Add 3 milligrams of the antibiotic streptomycin and stir until... The ingredients go well together. J. Divide the food into 150 x 16 mm test tubes that have been sterilized using an autoclave. Seal the tube with a cotton stopper to prevent contamination. f. Leave it to stand for 24 hours to allow the food to solidify and check for contamination.