A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung.

Abstract

OCR 10KL (27 / 11 / 2568) A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. According to this invention, the process involves the following steps: Dissolving elephant dung in a phosphate buffer saline solution. (Phosphate-buffered saline; PBS) and adjust the pH to the appropriate level, resulting in... Elephant dung solution was then mixed with basal medium for microbial culture. Then, mix it with sugarcane powder, stirring thoroughly while controlling the pH and temperature. Under suitable conditions, a nitrogen atmosphere is applied to extract cellulose fibers from modified sugarcane bagasse. Using microorganisms from elephant dung combined with simulations of the elephant's large intestine, the resulting cellulose fibers were then used. It is bleached with sodium chloride (NaClO2) and the pH is adjusted with acetic acid. The process involves altering the cellulose fibers with acid until white cellulose fibers are obtained. The final step is to wash the fibers with distilled water and dry them. Through heat, pure cellulose fibers are obtained, representing a utilization of elephant dung, among other benefits. It helps to maximize the value of bagasse, a byproduct of sugarcane and sugar factories, and reduce costs. In the cellulose extraction process, it also reduces the number of bleaching steps in the fiber process, thus reducing the amount. The use of harsh chemicals (sodium chloride (NaClO2)) in the bleaching process is possible.

Claims

OCR 10KL (27 / 11 / 2568) 1. A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. The steps are as follows: A. Prepare elephant dung by selecting and collecting fresh dung, then storing it in... Temperature-controlled box maintaining a temperature of -10 to -20 degrees Celsius, equipped with an oxygen absorber. absorber) B. Take the elephant dung that has been preserved in step A and grind it using a crushing and mixing machine. (Stomacher) resulted in elephant dung that was semi-liquid in consistency. C. Dissolve the crushed elephant dung from step B in phosphate buffer saline solution. (Phosphate-buffered saline; PBS) in a ratio of 0.5 - 5 parts by weight of elephant dung to... Mix 2-20 parts by volume of phosphate-buffered saline (PBS) thoroughly. And adjust the pH to 5-7 to obtain a nutrient solution. d. Prepare the basal medium. (Basal medium) 1 liter contains 2 grams of peptone and sodium bicarbonate. (NaHCO3) 2 grams, yeast extract 0.1 grams, tryptone. 0.1 grams of sodium chloride (NaCl), 1 gram of dipotassium phosphate (K2HPO4). 0.04 g, potassium dihydrogen phosphate (KH2PO4), 0.04 g, magnesium sulfate. Heptahydrate (MgSO4•7H2O) 0.01 g, Calcium chloride hexahydrate (CaCl2•6H2O) 0.01 grams and 2 milliliters of Polysorbate 20 (Tween 20). Adjust the volume with distilled water to a total of 1 liter. Mix all ingredients thoroughly and adjust the pH level. Adjust the pH to 7.

4. Sterilize using an autoclave at 121 degrees Celsius. Heat at 15 pounds per square inch (0.84 pounds) for 15-20 minutes. Let it cool. The food is then ready. Culturing the bacteria in basal medium. D. Mix the elephant dung solution obtained from step C with Basal Medium culture medium. The medium obtained from step d. consists of 1-2 parts by volume of elephant dung solution to... Mix 5-30 parts of the culture medium by volume to obtain a mixed solution consisting of the solution. Elephant dung mixed with culture medium. f. Prepare bagasse powder by grinding the bagasse into a fine powder with a specific size. 34-36 mesh yields bagasse powder. B. Extract cellulose fibers from sugarcane bagasse by mixing the bagasse powder obtained from step F with... A mixed solution consisting of elephant dung solution combined with the culture medium obtained from step E in the following ratio: Compost 1-5 parts by weight of bagasse powder to 5-30 parts by volume of mixed solution. Stir well. Mix at a speed of 120 - 200 rpm, with a pH of 5 - 7, at the specified temperature. At 35-40 degrees Celsius under a nitrogen atmosphere for 22-26 hours, fibers are formed. cellulose B. Bleach the cellulose fibers by dissolving the cellulose fibers obtained from step C in water. The composition consists of 1-5 parts cellulose fibers by weight to 5-100 parts water by volume. Mix thoroughly, then add sodium chloride (NaClO2) in an amount of 1-5% by weight / volume. (%w / v) and adjust the pH to 3-5 using acetic acid. With a concentration of 80-100%, the bleaching reaction takes place at a temperature of 50-80 degrees Celsius. Then add sodium chloride (NaClO2) and acetic acid every hour until the desired consistency is achieved. White cellulose fibers J. Wash the bleached cellulose fibers from step C with distilled water to adjust the pH. The washing solution's pH is adjusted until it reaches 7, then it is dried. By heating at temperatures of 40-70 degrees Celsius for 12-48 hours, cellulose fibers are obtained. That is pure.

2. A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. According to claim 1, the optimal pH of the elephant dung solution is 6.

3. A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. Under Claim 1, the optimal pH value for the extraction of cellulose fibers from sugarcane bagasse is specified. Equals 6.

4. A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. According to Claim 1, the optimal temperature for extracting cellulose fibers from bagasse is 39 degrees. Celsius 5. A process for producing cellulose fibers from sugarcane bagasse treated with microorganisms from elephant dung. According to claim 1, the optimal pH for bleaching cellulose fibers is 4.