Method for extracting phycocyanin from spirulina powder (Arthrospira sp.)

Abstract

The method for extracting phycocyanin from spirulina powder (Arthrospiro sp.) involves taking the powder... Dried spirulina algae (Arthrospiro sp.) are mixed with a sodium phosphate buffer solution (Na-phosphate). The extract was then treated with a buffer pH 7.0, followed by freezing and thawing, and the cell residue was filtered out. Phycocyanin is extracted using multipurpose tissue paper. The phycocyanin is then processed further. Purified using activated charcoal powder.

Claims

OCR 1. Method for extracting phycocyanin from spirulina powder (Arthrospira sp.). Where the steps are as follows: A. Mix 1 gram of spirulina powder (Arthrospira sp.) with a sodium phosphate solution. Add 10 milliliters of 0.1 molar Na-phosphate buffer (pH 7.0) and then apply the solution. It was frozen at a temperature of -8 to -20 degrees Celsius for 12-18 hours, and then left to cool at 25-30 degrees Celsius. Celsius degrees to melt. Then freeze the solution as described above and let it thaw. Repeat the process a certain number of times. 3 rounds B. Take 50-60 sheets of multipurpose tissue paper, cut into circles, and fill a syringe with them. Pour the solution obtained from step A into the 50 ml syringe, then inject the solution through it. Multipurpose tissue paper will yield a phycocyanin solution. C. Pour the phycocyanin solution obtained from step B into a test tube, then add the charcoal powder. Add 1000 milligrams of activated charcoal, then invert the vial or shake gently. For 10 minutes, to further purify phycocyanin. D. Filter the phycocyanin solution obtained from step C using tissue paper. Multipurpose: Take 50-60 sheets of multipurpose tissue paper, cut into circles, and fill a syringe. (Syringe) Use a 50 ml syringe and pour the solution obtained from step C into the syringe. Then, inject the solution through the syringe. Multipurpose tissue paper will yield a phycocyanin solution of food-grade quality. (Food Grade) Step B involves filtering the phycocyanin obtained from step D using multipurpose tissue paper. The process involves placing 25-30 sheets of multipurpose tissue paper, cut into circles, into a syringe. Pour 50 milliliters of the solution obtained from step d into a syringe, then force the solution through the paper. Multipurpose tissue paper will yield a phycocyanin solution.