Microfluidic sperm cell counting and sorting device.

Abstract

This invention relates to a device for counting and sorting sperm cells using a microfluidic system. This includes a sperm cell selection system, a real-time sperm cell detection and counting system, and... Sex separation of sperm cells and a sex-separated sperm storage system. This device according to the invention can select... Healthy sperm cells enter the sperm cell sorting system for further selection of higher quality sperm cells. This includes sperm cells that have been separated but retain their quality and viability, making them suitable for use in assisted reproductive surgery. Continue.

Claims

OCR 10KL (02 / 04 / 2568) 1. Microfluidic sperm cell counting and sorting device, consisting of a sperm cell selection system. (1) Real-time sperm cell detection and counting system (2) Sperm cell sex sorting system (3) and storage system Sexed sperm cells (4) Its unique characteristic is that it uses a microfluidic system to count and sort sperm cells. Where the sperm sex sorting system (3) is provided, it consists of a set of electrodes (32-33) which are placed parallel to the peritoneal cavity. Sperm cell flow (31) and magnetic particle-labeled monoclonal antibody loading channel (35).

2. A microfluidic sperm cell counting and sorting device, as per claim 1, in which an electrode assembly is used. (32-33) It is made up of multiwalled carbon nanotubes with a thickness of 0.5-1.5 mm and Conductive metal electrodes, which can be selected from platinum, gold, silver, titanium, chromium, and copper.

3. Microfluidic sperm cell counting and sorting device as per claims 1-2, where an electrode assembly (32-33) placed parallel to the sperm flow channel (31), the distance between the poles is in the range of 50-200. micrometer 4. A microfluidic device for counting and sorting sperm cells, as per claims 1-2, where the size of the cavity... Sperm cell flow (31) of the sperm sex sorting system is provided to have a length of 1,000-5,000. The size is in micrometers, and the width is in the range of 20-200 micrometers.

5. A microfluidic system for counting and sorting sperm cells, as per claim 1, whereby the selection system... Sperm cell (1) is arranged to contain the sperm cell loading channel (11), the culture medium loading channel (12), and the flow channel. Sperm cells (14) Culture medium flow channel (15) Main flow channel (16) Sperm cell flow channel Healthy (17) and inanimate sperm cell storage chambers (13).

6. A microfluidic sperm cell counting and sorting device as per claims 1 and 5, where the flow channel... The sperm cell (14) and culture medium flow channels (15) are at an angle of 30 - 50 degrees.

7. A microfluidic system for counting and sorting sperm cells, as per claim 1, whereby the system detects and... Real-time sperm count (2) provided with a set of single sperm flow channels (21) Electrodes (22) and processing system (23) consisting of an LCR meter (24) and Real-time processing programs (25) 8. A microfluidic device for counting and sorting sperm cells, as per claims 1 and 7, where the width... The electrode pair (22) ranges from 10-50 micrometers.

9. A microfluidic system for counting and sorting sperm cells, pursuant to claim Patent 1, whereby a cell storage system is provided. Sexed sperm (4) are arranged to consist of a sperm cell storage chamber containing the X chromosome (41) and a sperm cell flow channel. Contains an X chromosome (44), a sperm cell storage chamber containing a Y chromosome (42), and a sperm cell flow chamber containing a Y chromosome (45). and waste storage compartment (43)

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