A method for extracting important substances from discarded or substandard sea grapes.

Abstract

------27 / 03 / 2569------(OCR) The OCR 10KL method for extracting active compounds from discarded or substandard sea grapes, as described in this invention, consists of several steps. The raw material preparation involves drying and grinding fresh sea grapes to a particle size of 300 to 1000 microns for extraction. The extraction process then proceeds as follows: First, lipid or ethanolic extraction is performed from dried sea grapes using an ethanol solution. The sea grape sediment is separated by filtration, and the lipid or ethanolic extract is then evaporated to dryness. Next, polysaccharides are extracted from dried sea grapes and / or the remaining sea grape residue after lipid drying or wetting using hot water. The resulting polysaccharide solution is concentrated by evaporating water. The concentrated polysaccharide extract can be used directly or precipitated by adding an ethanol solution, after which the polysaccharide precipitate is dried. ------------ ------12 / 12 / 2568------(OCR) OCR 09WP This invention describes a method for extracting active compounds from discarded or substandard sea grapes. The process involves preparing the raw material by drying and grinding fresh sea grapes to a particle size of 300 to 1000 microns for extraction. The method then proceeds to extract one active compound (lipid / polysaccharide) or two active compounds, in sequential order: First, lipid or ethanol extract is obtained from dried sea grapes using an ethanol solution. The sea grape sediment is separated by filtration, and the lipid or ethanol extract solution is then evaporated to dryness. Next, polysaccharide is extracted from dried sea grapes and / or the remaining sea grape residue after lipid drying or wetting using hot water. The resulting polysaccharide solution is concentrated by evaporating water. The concentrated polysaccharide extract can be used or precipitated by adding an ethanol solution, after which the polysaccharide precipitate is dried.

Claims

------27 / 03 / 2569------(OCR) OCR 10KL1. The process of extracting active compounds from discarded or leftover grape seaweed consists of: a. Preparation of grape seaweed raw material for extraction: Fresh grape seaweed, after washing, is dried in a hot air oven at 65°C for 24 hours or sun-dried until the cells are dry and the moisture content is only 10%. It is then ground using a grinder / blenderer to obtain dried grape seaweed powder. b. Lipid extraction process: The dried grape seaweed powder obtained from step a is extracted with an ethanol solution at a ratio of dried grape seaweed powder to ethanol in the range of 1:5 to 1:15 weight to volume (w / v). The extraction is stirred with a propeller rotating at a speed of 200 to 400 rpm at a temperature of 25 to 30°C for 10 to 30 minutes, and repeated 2 to 4 times. The resulting lipid solution and seaweed residue are then filtered using filter paper or synthetic membranes. The resulting lipid solution is then subjected to ethanol evaporation until dry, yielding the lipid extract product. The polysaccharide extraction process uses the dried grape seaweed powder obtained from step A and / or the seaweed residue obtained from step B.Dry or wet extraction is performed using hot water at a ratio of dry grape seaweed powder and / or seaweed residue to hot water in the range of 1:5 to 1:20 weight-to-volume (w / v). The extraction is stirred with a propeller at a speed of 200 to 400 rpm at a temperature of 80 to 95 °C for 60 to 90 minutes, and repeated 2 to 3 times. The seaweed precipitate is then separated using a centrifuge to obtain a polysaccharide solution and seaweed residue. The resulting polysaccharide solution is concentrated by evaporation to reduce the water content of the extract to 1-2 parts per 5 parts of the initial volume, resulting in a concentrated polysaccharide solution that can be used. Alternatively, the concentrated polysaccharide extract can be precipitated by adding 2 to 4 times the volume of ethanol solution of the extract volume and storing it at 4 to 10 °C for 6 to 18 hours, then centrifuging to separate the polysaccharide precipitate from the solution. The precipitation process was repeated twice. The polysaccharide precipitate was then dried to obtain the polysaccharide extract in dry powder form.