A method for increasing the amount of gamma-aminobutyric acid (GABA) in sealed bags of germinated brown rice using electron beam irradiation.

Abstract

OCR Method for increasing the amount of gamma-aminobutyric acid (GABA) in sealed bagged germinated brown rice. Germinated rice is prepared using electron beam irradiation. The main steps are: 1. Preparing a blend of three germinated brown rice varieties, including fragrant rice... Dried brown rice, germinated black rice, germinated jasmine rice, and other dried rice are packaged in sealed vacuum-sealed bags. 2. Irradiation. The electron beam determines the average radiation dose (absorbed dose) received by the product to be 1.0 kilogray. The maximum energy level is 10 megaelectron volts. This applies to the dried, germinated brown rice mix, packaged in sealed bags. At this radiation dose level, the electron beam has a high quality and quantity of gamma-aminobutyric acid (GABA). Up to 6.44 mg / 100 g dry weight, which is higher than the hot air drying method with its gamma-acid content. Minobutyric acid (GABA) is present at 3.76 mg / 100 g dry weight, approximately 1.7 times the normal level.

Claims

OCR 10KL (09 / 06 / 2568) 1. Method for increasing the amount of gamma-aminobutyric acid (GABA) in germinated brown rice mixed in sealed bags. Bonding is achieved through electron beam irradiation and involves the following steps: a) The recipe for preparing mixed sprouted brown rice consists of three varieties of sprouted brown rice: brown rice... The rice used is a blend of fragrant crab rice, sprouted black fragrant rice, and jasmine brown rice in a weight ratio of 1:1:

1. b) The preparation process for mixed sprouted brown rice consists of the step of weighing each rice variety to obtain the correct weight. Place equal amounts of each ingredient in a container. Add clean water in a ratio of 1 part brown rice to 2 parts water. Wash thoroughly. Clean and drain off any remaining water. Repeat this process three times. Then add clean water and mix in the sprouted brown rice. Mix in the same ratio. Place in a temperature-controlled incubator at 35-37 degrees Celsius for 5-6 hours. Once the time is up, drain the water and rinse the brown rice with clean water. Wrap it in 1-2 layers of cheesecloth and place it in a container. Loosely seal and incubate in a temperature-controlled incubator at 35-37°C for 20-24 hours, with washing. Change the clean water every 4-6 hours to reduce unpleasant odors and the accumulation of microorganisms. Remove the cheesecloth wrap and wash the seeds. You will get germinated brown rice seeds with root buds approximately 1.0 cm long. 1.5 millimeters c) Processing of mixed germinated brown rice using the hot air drying method, by taking a mixture of 3 types of germinated brown rice. The seeds are dried in a hot air oven at 100 degrees Celsius for 40-60 minutes to reduce moisture content. Sterilize the product by removing microorganisms after drying. The resulting sprouted brown rice mixture is then packaged in high-temperature polyethylene plastic bags. Low density, hermetically sealed in a vacuum system to preserve the quality of mixed sprouted brown rice. d) Electron beam irradiation is performed by mixing germinated brown rice and placing it in a sealed bag. The bags are sealed together in a vacuum system with equal packing size and density, and then exposed to an electron beam. Under the specified arrangement, the electron beam projected onto the product is positioned horizontally. The average absorbed radiation dose received by the product is set at 1.0 kilogray. At a maximum energy of 10 megaelectron volts, brown rice products will be obtained with a high amount of gamma-aminobutyric acid. High levels of amino acid (GABA), up to 6.44 mg / 100 g body weight.

2. Method for increasing the amount of gamma-aminobutyric acid (GABA) in sealed bagged mixed germinated brown rice. Closely related by electron beam irradiation, in accordance with claim 1, whereby the irradiation process provides a specific radiation dose level. The optimal average absorbed dose of the product is 1.0 kilogray, which is the level of absorbed radiation dose. The minimum dose received by the product is not less than 0.5 kilogray, and the maximum absorbed radiation dose level is... The maximum dose of the product is 1.5 kilograys, with a maximum energy level of 10 megaelectrons. Volts resulted in an increase in the amount of gamma-aminobutyric acid to a maximum level greater than that of mixed germinated brown rice. Processed using only hot air drying, the yield is 1.7 times reduced, and then stored at... Room temperature 25.0°C, plus or minus 3.0°C.

3. Method for increasing the amount of gamma-aminobutyric acid (GABA) in packaged germinated brown rice mix. Sealed by electron beam irradiation, as per claims 1 and 2, where the germinated brown rice product contains a certain amount of substances... High levels of gamma-aminobutyric acid (GABA) after electron beam irradiation can be detected by... The process of quantifying gamma-aminobutyric acid (GABA) using liquid chromatography. High-performance liquid chromatography (HPLC) using ethanol and... Potassium dihydrogen phosphate solution was used as a solvent, with cleaning agents such as sodium acetate and... 30% methanol is used as the mobile phase for the separation of gamma-aminobutyric acid (GABA) in a chromatographic column. High-performance liquid graphing enables highly accurate separation and analysis results.

Images