The process of producing animal feed from durian peel and mushroom spawn.

Abstract

The process of producing animal feed from durian peel and mushroom spawn involves washing the durian peel thoroughly. The durian peel is sliced ​​and dried in an oven, then ground and sterilized. Mushroom cultures are prepared by isolating and culturing mushrooms on a culture medium, then incubating at room temperature. Starter cultures are also prepared using millet seeds and incubated at room temperature. Next, rice bran, urea, ammonium sulfate (NH4), calcium carbonate (CaCO3), and magnesium sulfate (MgSO4) are added to the prepared durian peel mixture. The mushroom culture and water are added, mixed thoroughly, and incubated at room temperature. After incubation, water and Candida utilis yeast (prepared by culturing in liquid medium) are added to the fermented mixture. The mixture is stirred thoroughly and incubated again at room temperature. After the specified incubation period, it is dried using hot air. This process yields animal feed made from durian peel and mushroom cultures.

Claims

1. The process of producing animal feed from durian peel and mushroom spawn involves the following steps: A. Wash the durian peel thoroughly and cut it into pieces 3-7 cm in size and 0.5-1 cm thick. Then, proceed to... Dry the fibers in a hot air dryer at 50°C for 2-3 days. Then, reduce the size further using a grinder to obtain fine fibers. Finally, sterilize the fibers in a steam autoclave at 121°C. For 15 minutes. B. Prepare the mushroom spawn by selecting mushrooms from the Lentinus squarrosulus Mont. variety. One of the following mushrooms can be used: enoki mushroom, shiitake mushroom, king oyster mushroom, oyster mushroom, angel mushroom, or shiitake mushroom. Isolated mushroom cultures were collected and cultured on solid potato dextrose agar (PDA). The cultures were then incubated at room temperature for 3-7 days. Next, starter cultures were prepared using millet seeds and incubated at room temperature for 7-14 days, or until the mushroom mycelium completely covered the millet seeds. C. Prepare the Candida utilis yeast by culturing it from the inoculum collection chamber. Yeast extract peptone dextrose (YPD broth) liquid medium was incubated for a certain period of time. 18-24 hours D. Add 2-30% by weight of rice bran to the durian peels prepared in step A. Urea (1-10% by weight), Ammonium sulfate (NH4) (amount not specified). 1-10% by weight, limestone (Calcium Carbonate; CaCO3) in the amount of 0.5-5% by weight. Add 0.1-5% by weight of magnesium sulfate (MgSO4), then add the mushroom culture obtained from step B. Add water in an amount 1-2 times the weight of the durian shell to adjust the moisture content. Mix thoroughly and then ripen at... At room temperature for 4-8 days under solid-state fermentation. D. Take the fermented mixture containing fungi under dry culture conditions obtained from step D and add water to it. The solid-to-liquid ratio should be 1:5-20 by weight, then add 5-10% by weight of the yeast culture obtained from step C. Stir well and then incubate at room temperature for 2-4 days in liquid state. Fermentation) or semi-solid state fermentation, followed by water reduction or drying. By drying with hot air at a temperature of 50-70 degrees Celsius for 1-3 days, animal feed can be obtained from durian peels. And mushroom spawn.