USE OF PHOSPHO-KERATIN 8 AS A BIOMARK IN DETECTING DIVIDING CANCER CELLS

TR202109156BActive Publication Date: 2026-06-22KOC UNIVSI
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Patent Information

Application Number
TR202109156
Authority / Receiving Office
TR · TR
Patent Type
Patents
Current Assignee / Owner
Filing Date
2021-06-03
Publication Date
2026-06-22
Estimated Expiration
2041-06-03

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Abstract

This invention relates to a biomarker and its use for the detection of a biomarker in the cytokinesis phase of dividing cancer cells based on keratin 8 phosphorylation specific to cell division in cancer cells.
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Claims

21644.40 17 REQUESTS 1. A biomarker for dividing cancerous cells in a biological sample. It is a method for detecting its presence, - Obtaining a biological sample and extracting a biological extract from the sample. 5 preparation, - the presence of the biomarker in the biological extract in question determination by qualitative or quantitative methods – Here, the biomarker is a cancerous cell dividing in the cytokinesis phase. It includes steps that demonstrate that it is, and its feature is 10 The step of determining the presence of the biomarker is using a reagent, which is an antibody. to be carried out through and the antibody in question phosphorylates the S34 region in keratin-8 protein It is recognition.

2. It is a method according to Claim 1, the characteristic of which is that the biomarker in question is phospho- S34 is a keratin-8 protein.

3. A method according to either claim 1 or claim 2, and its characteristic is; Immunohistochemistry is the step of determining the presence of the biomarker, 20 immunofluorescence, mass spectrometry, ELISA, protein microarrays, and with at least one method selected from the western blotting group It is the realization of.

4. It is a method according to claim 1, and its characteristic is that 25 are phosphorylated from the S34 region. The presence of keratin-8 protein is an indication that the sample contains cancerous cells. It is an indicator.

5. It is a method according to Claim 4, and its characteristic is that it targets cancerous cells in the liver and bile ducts. tract, colon, ovary, prostate, lung, mesothelioma, breast, stomach, 30 This can be any of the following areas: kidney, pancreas, uterus, and cervix. 21644.40 18 6. Detection of the presence of phosphokeratin 8S34 in a sample indicates cancer. It is a kit for the identification of the subject, the characteristic of which is from Claims 1 to 5. It is carried out using a method from any of them.