6-Gingerol-Based Antimicrobial and Antifungal Disinfectant Compound

Abstract

The invention relates to a disinfectant composition containing 10% 6-Gingerol and exhibiting broad-spectrum antimicrobial and antifungal activity, intended for rapid disinfection of medical surfaces (patient rooms, non-critical medical instruments), hand hygiene (alcohol-free hand wipes, antimicrobial soaps), general environmental cleaning (homes, offices, schools, public transport areas), non-food industrial surface cleaning, and hygiene applications in healthcare facilities outside of operating rooms (polyclinics, laboratories).

Description

1 TARIFF 6-GINGEROL BASED ANTIMICROBIAL AND ANTIFUNGAL DISINFECTANT COMPOSITION Technical Area 5 The invention has applications in the medical and healthcare sector, in the production of cleaning and hygiene products, cosmetics, and in the personal care sector, in the veterinary hygiene and disinfection sector, plant-based plant-based phenolic compounds used in biotechnology and hygiene solutions It relates to the formulation of disinfectant compounds. 10 The invention is particularly useful for the rapid disinfection of medical surfaces (patient rooms, non-critical medical). (tools), hand hygiene (alcohol-free hand wipes, antimicrobial soaps), general environmental Cleaning (homes, offices, schools, public transport areas), non-food industrial surfaces. cleaning in healthcare facilities other than operating rooms (polyclinics, laboratories) 15 Broad-spectrum formula containing 10% 6-Gingerol for hygiene applications. It relates to disinfectant compounds that exhibit antimicrobial and antifungal effects. State of the Art Today, healthcare facilities, public living spaces, and everyday environments use these facilities. Bacterial and fungal infections commonly found on surfaces affect both the general public and the general population. It threatens both health and the effectiveness of healthcare services. Most current disinfectant products contain high concentrations of alcohol or synthetic 25 It contains chemicals. This can lead to skin irritation, toxicity, environmental pollution, and low yields. This leads to various problems, such as decreased efficiency at higher temperatures. Furthermore, some Microorganisms are developing resistance to common disinfectants. Therefore, Safer, more environmentally friendly, broad-spectrum, and effective in various temperature conditions. The need for alternative antimicrobial and antifungal products is steadily increasing. 30 Current practices include solutions for surface disinfection and hand hygiene. chemical disinfectants, usually containing high levels of alcohol, chlorine-based solutions, Synthetic antimicrobials such as quaternary ammonium compounds (QACs) and phenol derivatives This is achieved using agents. These products are effective against specific microorganisms. Despite demonstrating effectiveness, various technical and health-related issues may arise during use. 2 This creates problems. Alcohol-based products, in particular, can cause skin dryness and irritation. This is because, due to their volatile structure, they evaporate quickly and at low temperatures. Their effectiveness decreases. Furthermore, frequent use can cause dryness and irritation to the skin. Chlorine-based disinfectants, on the other hand, have strong odors, corrosive effects, and toxic side effects. Due to their potential for product creation, there are limitations in terms of user safety. 5 Chlorine-based disinfectants are corrosive due to their strong oxidative effects and can damage metals. They cause damage to surfaces, emit irritating odors, and produce toxic byproducts. They can form quaternary ammonium compounds that have a broad spectrum of action. However, there is a risk of microorganisms developing resistance with long-term use. They carry and their effects may decrease with long-term use. Also, these substances have 10 When environmental waste mixes with water, it can harm the ecosystem and lead to environmental accumulation. This can have negative impacts on biodiversity. The common shortcomings in all these systems in current applications are; natural and biocompatible. Lack of content, insufficient effect at low temperatures, rapid and broad-spectrum effect in 15 Occasionally, there are issues such as inability to deliver, risk of toxicity, skin irritation, and environmental sustainability. The situation is that new, naturally derived, safe and effective products suitable for both medical and daily use are available. This has highlighted the need for next-generation disinfectant formulations. In the literature review, 20 is given as an example of the known state of the technique. Document number WO9960081 can be provided. This document is chlorine-based and... This relates to stable disinfectant compositions containing quaternary ammonium. This compound is a formulation used particularly in detergents and cleaning products. However... The ingredients used in the formulation can develop resistance over time by some microorganisms. It is developing and its effectiveness decreases with long-term use. This situation is relevant to hospitals and 25 It can pose a serious public health problem in healthcare settings. Furthermore, quaternary... Ammonium compounds can cause skin irritation or respiratory sensitivity. In conclusion, the existence of the above problems and the inadequacy of the current solutions, This has made it necessary to make improvements in the technical field. 30 Purpose of the Invention The present invention eliminates the aforementioned disadvantages and contributes to the relevant technical field. 6-Gingerol-based antimicrobial and antifungal disinfectant 35 that brings new advantages It is related to its composition. 3 The main purpose of the invention is to create a product containing 10% 6-Gingerol for hygiene applications. Disinfectant compound exhibiting broad-spectrum antimicrobial and antifungal activity. to reveal. The aim of the invention is to develop a broad-spectrum antimicrobial and antifungal solution with natural and safe ingredients. The goal is to develop an effective disinfectant compound. Another aim of the invention is to neutralize microorganisms quickly due to its rapid effect. The goal is to develop a disinfectant compound that will achieve this result. Another aim of the invention is to create a multi-purpose surface suitable for use on medical and environmental surfaces. The goal is to develop a disinfectant compound that offers a disinfectant. Another aim of the invention is to create a skin-friendly and environmentally conscious product that can be used in hand hygiene products. The goal is to produce a harmless disinfectant compound. 15 Another aim of the invention is to create a device that can maintain its effectiveness even at low temperatures. The goal is to develop a disinfectant composition. Another aim of the invention is to develop 20 plant-based compounds that will delay or prevent the development of resistance. The goal is to develop a disinfectant compound. Another aim of the invention is to create a product that is free of alcohol or toxic chemicals and has low side effects. The goal is to develop a disinfectant composition. Another aim of the invention is to use a formulation containing 10% 6-Gingerol to target both bacteria and... a highly effective microbial reduction (99.7–99.8%) against fungi The goal is to develop a disinfectant composition. Another purpose of the invention is to improve hand hygiene and surface disinfection. The goal is to develop a disinfectant compound that has a usable structure. Another purpose of the invention is to create a product that can be used in various forms such as wipes, soap, and sprays. The goal is to develop a disinfectant composition. 35 Another purpose of the invention is to meet the general criteria set by the CDC / WHO for low-risk areas. 4 The goal is to develop a disinfectant composition that complies with the conditions of use. Another aim of the invention is to address technical shortcomings in existing systems, thereby... effective, safe and sustainable in terms of both user health and environmental sustainability. The goal is to develop a disinfectant compound that offers a viable disinfectant solution. 5 To fulfill all the purposes mentioned above and those that can be derived from the detailed explanation. The invention is for the rapid disinfection of medical surfaces, hand hygiene, and general environmental cleaning. Non-food industrial surface cleaning, in healthcare facilities outside of operating rooms. Used in hygiene applications and has broad-spectrum antimicrobial and antifungal effects. 10 The disinfectant composition shown is 6-Gingerol (active antimicrobial agent), pure water. This is related to the presence of a carrier solvent and citric acid (pH stabilizer). The invention also enables rapid disinfection of medical surfaces, hand hygiene, and general environmental applications. Cleaning, non-food industrial surface cleaning, healthcare outside of operating rooms 15 broad-spectrum antimicrobial used in hygiene practices in establishments and is a method of producing disinfectant compounds that exhibit antifungal effects, a) 6-Gingerol is available in powder (crystal) or concentrated liquid form. by testing its purity (≥98%) in a laboratory environment before use. verification, 20 b) A suitable volume of pure water is placed in a heat-resistant glass container and heated, and 6- Gingerol is slowly added to the hot solvent while stirring. c) By adding citric acid to the solution and measuring the pH value with a pH meter. adjustment, d) Cooling the solution to room temperature and checking homogeneity and stability 25 to be kept waiting in order to provide e) Filtering the resulting mixture to remove solid particles. f) Filling the final product into sterile spray bottles using an automatic filling system. This includes the steps of sealing and labeling and storing the product. It is related. 30 Detailed Description of the Invention This detailed description outlines the preferred disinfectant composition and production method of the invention. The alternatives suggested are solely for the purpose of better understanding the subject and none of them are 35 It is explained in a way that will not create a limiting effect. The disinfectant composition subject to this invention consists primarily of: 6-Gingerol (active antimicrobial agent), It contains pure water (carrier solvent) and citric acid (pH stabilizer). Table 1 shows the composition and usage rates of the disinfectant compound that is the subject of the invention. Table 1: Disinfectant Composition, Content, and Usage Rates Contents Preferred by Weight Amount received (%) By weight Available Amount (%) 6-Gingerol (Active Antimicrobial Agent) 10 5-10 Pure Water (Carrier Solvent) 89 85-95 Citric Acid (pH stabilizer) 1 0.1-2 6-Gingerol (Active Antimicrobial Agent): 10 6-Gingerol, a naturally occurring phenolic compound derived from ginger, has powerful antimicrobial properties. It possesses the following characteristics: it is effective against Gram-positive and Gram-negative bacteria and yeast species. It is effective. In the invention, it is used at a concentration of 10% to disrupt the cell membrane of microorganisms. and by halting metabolic processes, rapid and broad-spectrum microbial It provides inhibition. Furthermore, thanks to its low toxicity, it is effective on the skin and surface. 15 It is safe in its applications. Pure Water (Carrier Solvent): A biologically inert and non-toxic material that dissolves the active ingredient and ensures a homogeneous distribution. It is a non-carrier material. It is skin-compatible and supports chemical stability. 20 It was preferred because 6-Gingerol remains effective on the surface for the desired contact duration. It ensures it stays put. Citric Acid (pH Stabilizer): By stabilizing the pH level of the formulation, it both preserves the microbial-friendly environment and 25 It prevents irritation upon contact with the skin. It also has properties that support antimicrobial effects. In particular, to limit the tendency of bacteria to multiply in alkaline environments, neutral or It provides a slightly acidic environment. 6 The production method of the disinfectant compound that is the subject of this invention is as follows: a) Procurement and preparation of raw materials: 6-Gingerol is available in powder (crystal) or concentrated liquid form. Purity (≥98%) determined by analytical methods such as HPLC or FTIR before use. 5 It is verified in the environment. b) Dissolving 6-Gingerol: A suitable volume of pure water is placed in a heat-resistant glass container and heated to 40–45 °C. It is heated to a temperature of 10 g. 10 g of 6-Gingerol is weighed out and slowly added to the hot solvent. The mixture is heated to 10 Stir with a magnetic stirrer for 30 minutes. If complete dissolution is not achieved, A homogeneous suspension form is acceptable. c) pH adjustment: 1 g of citric acid is added to the solution, and the pH value is measured with a pH meter to bring it to the range of 5.5–6.5. This process improves the product's safety upon contact with skin and its chemical stability. supports. d) Cooling and stabilization: The solution is cooled to room temperature (25 ±2 °C) and homogeneity and stability are checked at 20°C. It is left to sit for 24 hours to allow it to ferment. e) Filtration: The resulting mixture is filtered through a 0.45 micron filter to remove solid particles. f) Packaging: The final product is filled into sterile bottles with spray caps using an automated filling system, and then sealed. It is labeled and stored. The disinfectant compound described in this invention disrupts the cell membrane and enhances quorum sensing. It provides antimicrobial and antifungal effects by inhibiting infection. Disinfectant formulations come in various forms, including sprays, wipes, gels, or soaps. Applicable. Maintains microbial activity without the use of alcohol or harsh solvents. 1–5 It is effective even with short contact times, such as minutes. It achieves ≥99.9% reduction even at low temperatures. It provides stable antimicrobial activity at 5°C, 25°C and 37°C and maintains a cold chain. 7 It can also be used in outdoor environments and disorganized storage areas. The disinfectant composition described in the invention exists in both liquid and semi-solid (gel) forms. It is designed to be used in various ways. For example, as a spray for surface disinfection. It can be applied as a gel or in gel form for hand hygiene. Application method, needs 5 It varies depending on the duration of contact and the environment in which it is used. A short period, such as 1-5 minutes. It is effective within a short period of time and its effectiveness extends over a wide temperature range from 5°C to 37°C. Thanks to its protection, it can be safely used in different climatic conditions and industrial environments. It is available for use. Application examples of the disinfectant composition that is the subject of this invention: • Hospital and Clinic Surfaces: Alcohol-based products may cause skin irritation. or in environments where they may pose a fire risk, patient beds, examination rooms It can be used as a disinfectant spray on surfaces such as tables and doorknobs. 15 • Hand Wipes: Alcohol-free personal hygiene wipes. implemented by healthcare personnel or public transport users available. • Antifungal Foot Sprays: The strong antifungal effect of the disinfectant composition protects the feet. Suitable for the prevention and treatment of fungal infections. 20 • Environmental Disinfection: Such as in schools, waiting rooms, and gymnasiums. It can be applied in low-risk areas via ambient spray or mist. • In cattle and poultry shelters, veterinary clinics, and animal shelters to control microbial contamination in transport equipment applicable. 25 • Such as greenhouse equipment, product storage areas, and post-harvest processing surfaces. It can improve plant health and biosecurity by providing disinfection in environments. Application method and points to consider for the disinfectant composition that is the subject of this invention: • Before applying disinfectant, the area to be treated (skin, surface or The equipment should be cleaned of dirt and grease with water or a mild, alcohol-free cleaner. • Disinfectant should be applied directly to the surface or skin from a distance of approximately 10–15 cm. It is applied by spraying. • When applied to skin surfaces such as hands, the product should be gently massaged in for at least 30 seconds. It should be spread by rubbing. 8 • After application, it should be allowed to dry naturally on the surface. It should not be wiped away. This ensures the necessary contact time (1-5 minutes). • When used on medical instruments or sensitive equipment, contact time Rinsing with sterile water afterwards is recommended. • If the disinfectant solution is in suspension form, rinse the bottle 5 times before each use. It should be shaken well. Points to consider during application: • Should not be used on open wounds or cracked skin. 10 • Avoid contact with eyes, mouth, and mucous membranes. • Store in a cool, dry place, away from direct sunlight. should be done. • For external use only; do not swallow. The disinfectant composition that is the subject of this invention is effective against Escherichia coli and Pseudomonas aeruginosa. Against a wide range of pathogens such as Staphylococcus aureus and Candida albicans. It exhibits a strong and long-lasting antimicrobial effect. The technical evaluation of the invention is presented below with experimental data and visuals. 20 Supported by: Figure 1 shows the microorganisms exposed to 10% 6-Gingerol at 0, 1, 5 and 60 minutes. It then shows the decrease in colony growth. The image in each panel, This clearly demonstrates a decrease in microbial load; in particular, Candida albicans 25 Its sensitivity is pronounced. Figure 2 shows a graph illustrating the decrease in log₁₀. Figures 3 and 4 show the survival curves and D-value calculations for 30 The formulation reveals its time-dependent killing effect and temperature resistance. It places. Broad-spectrum antimicrobial effect: The disinfectant composition containing 10% 6-Gingerol provides both Gram-positive and gram-negative bacteria (E. coli, P. aeruginosa, S. aureus) and fungi 35 It is effective against Candida albicans species, providing comprehensive biological safety. 9 Fast and effective: Microorganisms are eliminated with only a short contact time of 1–5 minutes. It can reduce it by up to 99.9% (≥3-log) in a short period of time. This is due to rapid intervention. It saves time and resources in the areas where it is needed. Alcohol and chlorine-free formula: Eliminates chemical risks such as skin irritation, flammability, and toxicity. The side effects caused by disinfectants have been eliminated. This is especially true for sensitive individuals. This offers a great advantage for people with skin problems and for indoor spaces. Stable effect at low temperatures: The disinfectant compound remains stable at temperatures as low as 5°C for 10 minutes. Its effectiveness makes it suitable for use in cold climates and cold chain applications. increases. Environmentally friendly and sustainable: It is biodegradable thanks to its plant-derived (gingerol) content. It does not harm the environment. It contributes to sustainable cleaning solutions. 15 Multipurpose use: Both in medical fields (surface disinfection, hand hygiene) and Personal care (wipes, sprays, gels) and environmental cleaning (room sprays, agricultural surfaces) suitable for.

Claims

REQUESTS 1. Rapid disinfection of medical surfaces, hand hygiene, general environmental cleaning, food. exterior industrial surface cleaning, in healthcare facilities outside of operating rooms used in hygiene applications and is a broad-spectrum antimicrobial and 5 It is a disinfectant compound that exhibits antifungal effects; its characteristic is: - 6-Gingerol (active antimicrobial agent), purified water (carrier solvent), citric acid It contains a (pH stabilizer).

2. A disinfectant composition conforming to Claim 1, with the characteristic of being 5-10% by weight. 6-Gingerol (active antimicrobial agent) at a concentration of 85-95% pure by weight. water (carrier solvent), 0.1-2% by weight of citric acid (pH stabilizer) It includes.

3. Rapid disinfection of medical surfaces, hand hygiene, general environmental cleaning, food 15 exterior industrial surface cleaning, in healthcare facilities outside of operating rooms broad-spectrum antimicrobial agents used in hygiene applications. It is a method of producing disinfectant compounds that exhibit antifungal effects, and its characteristic feature is; a) 6-Gingerol, in powder (crystal) or concentrated liquid form. obtained and its purity (≥98%) is tested in the laboratory before use. verification in the environment, b) A suitable volume of pure water is poured into a heat-resistant glass container. heating and slowly adding 6-Gingerol to the hot solvent by being mixed, c) Adding citric acid to the solution and measuring the pH value with a pH meter until 25 adjustment, d) Cooling the solution to room temperature and ensuring homogeneity. to be held in order to ensure stability, e) Filtering the resulting mixture to remove solid particles. f) The final product is automatically filled into sterile spray bottles by an automated filling system. 30 filling, sealing and labeling and storing It includes the steps of the process.

4. A method for producing a disinfectant compound that complies with Claim 3, and whose characteristics are: Heating pure water to a temperature of 40–45 °C in the mentioned (b) process step 35 It includes the process step. 11 5. A method for producing a disinfectant compound that complies with Claim 3, and whose characteristics are: In the process step mentioned (b), the mixture is stirred with a magnetic stirrer for 30 minutes. This involves the mixing process step throughout.

6. A method for producing a disinfectant compound in accordance with Claim 3, characterized by: In the aforementioned step (c), the pH of the solution is brought to the range of 5.5–6.

5. It includes the adjustment process step.

7. A method for producing a disinfectant composition in accordance with Claim 3, with the following characteristic: 10 In the process step (d) mentioned, the solution is cooled to 25 ±2 °C. It includes the step.

8. A method for producing a disinfectant compound in accordance with Claim 3, characterized by: In the mentioned step (d), the solution is left to stand for 24 hours, which is step 15. It includes.

9. A method for producing a disinfectant compound in accordance with Claim 3, characterized by: In the aforementioned step (e), the mixture is filtered through a 0.45 micron filter. It includes the process step. 20

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