Phospholipid composition containing surfactants and its use.

Abstract

According to this invention, the phospholipid composition containing a surfactant may include a lysophospholipid and a sulfosuccinate-based surfactant; thus, the active ingredient, lysophospholipid, is well dispersed even in an aqueous solution, and its effectiveness is preserved for a long period, even when stored for extended periods, by preventing easy degradation of the active ingredient. Therefore, the phospholipid composition of this invention is commercially more readily available compared to conventional pesticide compositions with short shelf lives, as it suppresses the degradation of the active ingredient, lysophospholipid, allowing for long-term storage of the product.

Description

1 TARIFF Phospholipid composition containing surfactant and its USAGE THE FIELD OF INVENTION

[0001] This invention relates to a phospholipid compound, described in detail as a surfactant. It relates to a phospholipid composition containing... BACKGROUND OF THE INVENTION

[0002] Lysophosphatidylethanolamine (LPE), animal and plant It is naturally found in cells, especially egg yolks and brain cells. It is contained in high amounts. Lysophosphatidylethanolamine is found in the cell membrane. phosphatidylethanolamine, a type of phospholipid found It is derived from phosphatidylethanolamine (PE) in egg yolk or soy lecithin. Phosphatidylethanolamine, which is abundant, is a type of phospholipid that contains two lipids within its molecule. It contains acid. In living organisms, phosphatidylethanolamine is a phospholipid hydrolysis enzyme. As a result of the removal of a fatty acid through the action of phospholipase A2. It is converted to lysophosphatidylethanolamine (LPE).

[0003] Lysophosphatidylethanolamine (LPE) in the ripening of fruits and It is known to play a very important role in aging (senescence). The application of lysophosphatidylethanolamine to tomato leaves and fruits It is known to suppress aging, and is applied to tomatoes after harvest. It is also known to play a role in extending the storage life of the fruit in this situation. Furthermore... When applied to apples, it stimulates the formation of anthocyanins in the peel and loss of softening during storage of harvested apples It is known to have a suppressive effect. (Previous Patent Documents 1 and 2 in the Art: US) patent 5,110,341 and US patent 5,126,155)

[0004] In this way, lysophosphatidylethanolamine (LPE) is quite useful in the field of agriculture. Although it is a substance used in this way, the fatty acid in its chemical structure is linked to... The ester bond resists hydrolysis reactions that occur with the addition of water molecules. 2 It is very sensitive; therefore, maintaining a stable formulation in aqueous solution for a long time is crucial. One disadvantage is that it is difficult to store. It is currently sold commercially. In the case of a 10% aqueous solution of lysophosphatidylethanolamine (LPE), the temperature is usually 20 °C. Precipitation formation at low temperatures or below, or at room temperature or above. At these temperatures, the phenomenon of rapid decomposition of the active ingredient occurs. That is, it resists hydrolysis. Lysophosphatidylethanolamine (LPE), which has a delicate chemical structure, is the active ingredient in the product. Increasing its content as a component while having a long shelf life is quite commercially advantageous. It is important, but so far there is no plan to effectively solve the problem. It still seems very far away. OBJECTIVES OF THE INVENTION

[0005] In order to solve the problems mentioned above, this invention aims to solve The intended goal is a water-miscible phospholipid compound containing a surfactant. to provide.

[0006] In order to solve the problems mentioned above, this invention aims to solve Another aim it sought was to use phospholipid composition to treat a plant or a part of a plant by applying a treatment to that part, its health status, growth or life The goal is to provide a method to change the life cycle.

[0007] In order to solve the problems mentioned above, this invention aims to solve Another goal it aimed for was a water-miscible phospholipid containing a surfactant. The aim is to provide various uses for its composition.

[0008] On the other hand, the technical purposes of this invention are the same as the technical purposes stated above. Other technical purposes not specified, but not limited to those mentioned, are included in the explanations below in this area. This will be clearly understood by experts. SUMMARY OF THE INVENTION

[0009] In order to solve the above objective, one aspect of this invention is as follows: A lysophospholipid, represented by the chemical formula 1, and the following: A sulfosuccinate-based surfactant, indicated by Chemical Formula 2. It may contain a phospholipid compound containing the substance.

[0010] [Chemical Formula 1] 3

[0011]

[0012] In the above Chemical Formula 1, R1; C5 to C24 are saturated or unsaturated. chain-structured asilox group, C5 to C24 saturated or unsaturated chain-structured alkox group and any of its derivatives,

[0013] R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structure between the asilox group and the C1 to C5 saturated or unsaturated chain alcox groups It is anyone who is chosen,

[0014] R3; selected from hydrogen, choline, ethanolamine, glycerol, inositol and serine. anyone,

[0015] [Chemical Formula 2]

[0016]

[0017] In Chemical Formula 2 above, R4 and R5 are each hydrogen, sodium or C1 to C18 saturated or unsaturated chain structured or branched structured alkyl It is a group; however, neither R4 nor R5 are hydrogen or sodium.

[0018] The derivative in question contains at least one or more of the chemical structures of the R1 in question. a derivative in which at least one or more hydroxyl groups are attached to more carbon atoms it could be. 4

[0019] The phospholipid compound in question is miscible with water in liquid or powder form. It could be a combination.

[0020] The concentration of the lysophospholipid in question, the phospholipid composition in question It can be between 0.1% and 20% by weight, depending on the total weight.

[0021] The content of the sulfosuccinate-based surfactant in question, The phospholipid composition can be included at a rate of 0.1% to 20% by weight, depending on the total weight.

[0022] The phospholipid composition in question is C6 to C24 saturated or unsaturated chain structured. fatty acid; any metal salt chosen from among Na, K, Mg, Ca, and Al. fatty acid metal salts containing; ethanolamine salt, ammonium salt, betaine, choline salt fatty acid organic salt containing any organic salt selected from among them; or a combination of two or more of these; a fatty acid additive containing It may include more.

[0023] The content of the said fatty acid additive is the said phospholipid It can be included by weight at a rate of 1% to 20%, based on the total weight of the composition.

[0024] The phospholipid compound in question is ethanol, propanol, butanol, hexanol, octanol and including at least one or more of these selected from among combinations monohydric alcohol; 1,3-butanediol, 2,3-butanediol, propanediol, hexanediol, octanediol and dihydric compounds containing at least one or more of these, selected from among their combinations alcohol; trihydric alcohol containing glycerol; and a mixture of two or more of these. It may also contain an organic solvent.

[0025] The phospholipid composition in question is lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and two or more of these. It may include more than one combination.

[0026] In order to solve the other purpose mentioned above, a plant or a part of a plant part of it treated with a plant treatment agent containing the phospholipid compound in question by assessing the health and growth of the plant or part of the plant in question. or it may include a method involving a step to modify the life cycle.

[0027] The phospholipid compound in question is represented by the following Chemical Formula 1. lysophospholipid, represented by the following Chemical Formula 2. It may contain a sulfosuccinate-based surfactant.

[0028] [Chemical Formula 1]

[0029]

[0030] In the above Chemical Formula 1, R1; C5 to C24 are saturated or unsaturated. chain-structured asilox group, C5 to C24 saturated or unsaturated chain-structured alkox group and any of its derivatives,

[0031] R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structure between the asilox group and the C1 to C5 saturated or unsaturated chain alcox groups It is anyone who is chosen,

[0032] R3; selected from hydrogen, choline, ethanolamine, glycerol, inositol and serine. anyone,

[0033] [Chemical Formula 2]

[0034]

[0035] In Chemical Formula 2 above, R4 and R5 are each hydrogen, independently, sodium or C1 to C18 saturated or unsaturated chain structured or branched structured alkyl It is a group; however, neither R4 nor R5 are hydrogen or sodium. 6

[0036] The derivative in question contains at least one or more of the chemical structures of the said R1. a derivative in which at least one or more hydroxyl groups are attached to more carbon atoms it could be.

[0037] The plant treatment agent in question contains 1 to 5,000 ppm of the lysophospholipid in question. It can be an aqueous solution in which it is included at a (w / w) concentration.

[0038] Parts of the plant in question; fruit, leaf, flower, root, stem and tuber There may be at least one or more selected from among them.

[0039] The phospholipid compound in question is miscible with water in liquid or powder form. It could be a combination.

[0040] The plant treatment agent in question is lecithin, hydroxylated lecithin, acetylated Among lecithin, enzyme-treated lecithin, and combinations thereof, the best option is selected. It may include one or more additional items.

[0041] The method in question improves the quality of a plant or part of a plant. It could be aimed at improvement.

[0042] The quality in question includes color, flavor, firmness, sugar content, fruit cracking and It may include at least one or more of the selected fragrances.

[0043] This method involves the aging of a plant or part of a plant. It could be aimed at delaying things.

[0044] Delaying the aging of a plant or part of a plant This may be aimed at extending storage time or shelf life.

[0045] This method stimulates the growth of a plant or part of a plant. the diameter, width, weight, and number of their fruits, vegetables, roots, and / or tubers It may aim to increase at least one or more of the selected items.

[0046] This method involves treating a plant or part of a plant biotically or abiotically. It may be aimed at protecting against stress.

[0047] The abiotic stresses in question are freezing, wind, hail, flood, drought, heat and agriculture. among the medications, at least one or more physical ailments resulting from abiotic causes. It may include damage. 7

[0048] The pesticides in question are plant growth regulators and insecticides (insecticides). pesticides, fungicides, herbicides, spreaders and adhesives, and It may include at least one or more of the selected fertilizers.

[0049] This biotic stress includes pathogens, insects, nematodes, snails, At least one of the following is present: mites, weeds, humans, and non-human animals. It can include physical damage resulting from biotic causes, involving more than just a few examples.

[0050] The pathogens in question are fungi, protozoa, bacteria and viruses. It may include at least one or more of the following.

[0051] This method involves extracting starch, protein, lipid from a plant or part of a plant. and the components selected from a combination of two or more of these. It may be aimed at increasing its synthesis. INDUSTRIAL APPLICABILITY

[0052] According to this invention, the phospholipid composition containing a surfactant is a lysophospholipid and because it contains a sulfosuccinate-based surfactant, the active ingredient is lysophospholipid. It ensures good dispersion even in an aqueous solution and remains stable even when stored for a long time. By ensuring that the active ingredient does not easily decompose, it maintains its effectiveness for a long period of time. It can protect. Therefore, the phospholipid composition of this invention protects the active component, lysophospholipid. By suppressing its decomposition, it allows the product to be stored for a long time, thus extending its shelf life. commercially easier compared to traditional pesticide formulations which are shorter It can be used in some way.

[0053] The effects of this invention are not limited to those stated above, but include those not stated. Other effects are clearly explained by experts in this field in the following statements: It will be understandable. BRIEF DESCRIPTION OF THE ATTACHED ILLUSTRATIONS

[0054] Figure 1 shows a long-term storage example of this invention. types of water-miscible phospholipid compounds with improved stability formulations These are the results of changes in residue levels during storage. 8 Figure 2 shows an application example of this discovery regarding the phospholipid composition of kiwi fruit for cancer treatment. This is a result from testing the control effect against bacterial ulcer disease. Figure 3 shows an application example of this invention using phospholipid composition by Hongro. Testing the suppressive effect against frost damage (cold damage) in apple varieties. It is a result of being done. Figure 4 shows an application example of this invention, harvesting using phospholipid composition. This is a result showing the change in apple peel color in the previous Hongro variety. Figure 5 shows an application example of this invention using phospholipid composition at Picnic. This is a result from testing the effectiveness of color enhancement in various apple varieties. Figure 6 shows an application example of this invention using phospholipid composition. A study on testing methods to promote coloring in Campbell Early grape variety. It is the result. Figure 7 shows an application example of this invention, 7 days after its production. Inducing coloration in Picnic variety apples using phospholipid compounds. It is a result of the testing. Figure 8 shows an application example of this invention, one year after its production. Measuring the change in apple peel color of the Picnic variety using phospholipid composition. It is a result of... Figure 9 shows an application example of this invention, 7 days after its production. Coloring is promoted in Campbell Early grape variety using phospholipid compounds. It is a result of testing its effectiveness. Figure 10 shows an application example of this invention, one year after its production. Changes in Campbell Early grape skin color using phospholipid composition It is a result of observation. DETAILED DESCRIPTION OF THE INVENTION

[0055] Various modifications can be made to this invention and it can have various forms. It is possible; in this respect, specific application examples are illustrated in the figures and in the text. This has been explained in detail. However, this does not limit this invention to a specific form of disclosure. This does not aim to... and all aspects within the philosophy and technical scope of this invention. 9 It should be understood that it includes modifications, equivalents, and alternatives. Each form In the description, similar reference numbers were used for similar structural elements.

[0056] Unless otherwise defined, including technical or scientific terms Therefore, all terms used herein are those of a specialist in the technical field to which this invention belongs. It has the same meaning as what is generally understood by the person. Commonly used Terms such as those defined in dictionaries, within the context of the relevant technology. It should be interpreted in a way that is consistent with its meanings, and in this application Unless explicitly defined, they are not interpreted in an idealized or overly formal sense.

[0057] Throughout this specification, any structural element of any section When "contains" is specified, this refers to other structures unless there is a record to the contrary. This does not mean that the elements are excluded, but rather that other structural elements are included. It means it may contain.

[0058] The degree of "approximately", "essentially" etc. used throughout this specification The terms indicating this numerical value are presented when a material tolerance related to the meaning in question is offered. It is used in a sense equivalent to or close to that numerical value, and this specification... Disclosure that includes precise or absolute numerical values ​​to aid understanding. to prevent its content from being unfairly used by malicious offenders It is used for this purpose.

[0059] In the context of this specification, any substance (solute), any When it is stated that the solute "disperses" or "dissolves" in the mixture (solvent), this indicates that the solute... dissolved in the solvent in a transparent, stable and homogeneous state without precipitation. It indicates that it is.

[0060] Phospholipid Composition

[0061] Registered as an agricultural pesticide in the United States In the case of a water-miscible preparation containing lysophosphatidylethanolamine (LPE), As storage temperature increases, the amount of lysophosphatidylethanolamine (LPE) increases over time. such as an increase in the decomposition rate and a decrease in the amount of remaining lysophosphatidylethanolamine (LPE). A problem exists. Specifically, within a month when the temperature is 80°C. Almost all of the decomposition of lysophosphatidylethanolamine (LPE) takes place. On the other hand, Although product stability is maintained to a degree for up to 120 days at room temperature, the product Normally, 20% or more after 6 to 8 months from production. Due to the loss of lysophosphatidylethanolamine (LPE), a shelf life of 1 year or longer is prohibited. There is a situation where it is difficult to sustain its lifespan. In contrast, this invention... The preferred phospholipid composition is based on lysophospholipid and sulfosuccinate, which are the active ingredients. Because it contains a surfactant, it is miscible with water, its dispersion in the solvent is improved, and it is active. This results in a suppression of component decomposition effect; therefore, at least 2 years or more. A long shelf life can be ensured.

[0062] First, the phospholipid composition of this invention is given by the following Chemical Formula 1. It may contain a lysophospholipid, which is shown.

[0063] [Chemical Formula 1]

[0064]

[0065] In the above Chemical Formula 1, R1; C5 to C24 are saturated or unsaturated. chain-structured asilox group, C5 to C24 saturated or unsaturated chain-structured alkox group and any of its derivatives; R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structured asilox group and C1 to C5 saturated or It is any of the alkox groups with an unsaturated chain structure, and R3 is hydrogen. It can be any one of the following: choline, ethanolamine, glycerol, inositol, and serine.

[0066] Specifically, R1 refers to C5 to C24 saturated or unsaturated chains. R2 is any one of the asilox group structures and their derivatives. It is a hydroxyl group, and the R3 in question could be an ethanolamine.

[0067] More specifically, R1, C5 to C24 in question are saturated or unsaturated. It is a chain-structured acylox group, containing a saturated fatty acid or fat that does not have a double bond. an unsaturated fat that has at least one or more double bonds in the middle of its acid chain It can refer to a fatty acid. R1, C5 to C24, is a saturated fatty acid, in other words... an acylox group with a saturated carbon chain, pentanoilox being an example. group (pentanoyloxy), hexanoylox group (hexanoyloxy), heptanoylox group 11 (heptanoyloxy), octanoylox group (octanoyloxy), nonanoylox group (nonanoyloxy), decanoylox group (decanoyloxy), undecanoylox group (undecanoyloxy), dodecanoylox group (dodecanoyloxy), tridecanoylox group (tridecanoyloxy), tetradecanoylox group (tetradecanoyloxy), pentadecanoylox group (pentadecanoyloxy), hexadecanoylox group (hexadecanoyloxy), heptadecanoylox group (heptadecanoyloxy), octadecanoylox group (octadecanoyloxy), nonadesanoylox group (nonadecanoyloxy), aykosanoylox group (icosanoyloxy), heneicosanoylox group (heneicosanoyloxy), docosanoilox (docosanoyloxy), tricosanoylox (tricosanoyloxy), tetracosanoylox (tetracosanoyloxy) and It could be any of the derivatives chosen from among these. Furthermore, the aforementioned R1 is C5 to... C24 is an unsaturated fatty acid with an unsaturated carbon chain containing one double bond. It has an asilox group, an example of which is the pentenoylox group (pentenoyloxy), hexenoylox group (hexenoyloxy), heptenoylox group (heptenoyloxy), octenoylox group (octenoyloxy), nonenoylox group (nonenoyloxy), decenoylox group (decenoyloxy), undecenoylox group (undecenoyloxy), dodecenoylox group (dodecenoyloxy), tridecenoylox group (tridecenoyloxy), tetradecenoylox group (tetradecenoyloxy), pentadecenoylox group (pentadecenoyloxy), hexadecenoylox group (hexadecanoyloxy), heptadecenoyloxy group (heptadecenoyloxy), octadecenoyloxy group (octadecenoyloxy), nonadecenoylox group (nonadecenoyloxy), eicosenoylox group (icosenoyloxy), heneykosenoylox group (heneicosenoyloxy), docosenoylox (docosenoyloxy), trichosenoylox (tricosenoyloxy), tetracosenoylox (tetracosenoyloxy) and It could be any of the derivatives chosen from among these. Furthermore, the aforementioned R1 is C5 to... C24 is an unsaturated fatty acid with an unsaturated carbon chain containing two double bonds. is an asilox group, an example of which is cis,cis-9,12-octadecadienoilox (9,12- octadecadienoyloxy (derived from linoleic acid), cis, cis, cis-9,12,15-octadecadienoyloxy (cis,cis,cis-9,12,15-octadecatrienoyloxy, linolenic acid derived), arachidonoylox (arachidonoyloxy(eicosatetraenoyloxy), derived from arachidonic acid) and their derivatives It could be any one of them chosen from among them.

[0068] The point is that the plant surface is highly nonpolar and the active ingredient is in the plant to ensure good penetration from the surface or good adhesion of the active ingredient to the plant surface From the perspective of providing; preferably, for the R1 in question, C8 to C14, Saturated fats are preferably C8 to C12, with C10 to C12 being the most preferred. Acids can be used, but are not limited to. 12

[0069] In addition, the derivative in question contains at least the chemical structure of the R1 in question. a carbon atom in which at least one or more hydroxyl groups are attached to one or more carbon atoms It could be a derivative. In detail, the derivative in question is one or more of the R1 in question. to the highly unsaturated bond, for example, at the location of the double bond, at least one or more The reaction in which a hydroxyl group is attached, i.e., the hydroxylation reaction. It may contain a compound formed through this process, and the lysophospholipid formed in this way, It could be hydroxylated lysophosphatidylethanolamine (hydroxylated LPE). On the other hand, this situation... the chemical structure R3 in question contains at least one or more hydroxyl groups It can also mean binding or substitution. This refers to hydroxylation. (Hydroxylation) reaction, for example, hydrogen peroxide or a similar peroxide a chemical method in which the substance is used; or a lipid oxidation enzyme A biological method performed using lipoxygenase and similar agents; It can be carried out through this means.

[0070] More specifically, the Chemical Formula 1 in question is the following Chemical This can be represented by formula 3.

[0071] [Chemical Formula 3]

[0072]

[0073] In Chemical Formula 3 above, R1, C5 to C24 are saturated or unsaturated. It can be any of the following: a chain-structured alkyl group and its derivatives. Furthermore, the derivative in question contains at least one or more components within the chemical structure of R1. It could be a derivative in which at least one or more hydroxyl groups are attached to the excess carbon.

[0074] In a specific application example, Chemical Formula 3 in question, It may contain, but is not limited to, lysophosphatidylethanolamine (LPE).

[0075] The lysophosphatidylethanolamine (LPE) in question is a It is a type of lysophospholipid and is derived from phosphatidylethanolamine. (phosphatidylethanolamine, hereinafter referred to as PE) can be derived from glycerin. 13 its backbone contains two fatty acids, a phosphate group, and attached ethanolamine. From phosphatidylethanolamine (PE), one of the two fatty acids in question is located at the sn-2 position. It may have a chemical structure from which the fatty acid has been removed.

[0076] The lysophosphatidylethanolamine (LPE) in question is naturally found in plants and animals. Since it is a substance found in natural form, it is isolated and purified from natural substances. Those that can be used are especially lecithins from soybeans, egg yolks, or rapeseed. It can be isolated. Crude soybeans are produced as a byproduct in the soybean oil production process. lecithin (crude soybean lecithin, often referred to as raw lecithin); 60% to 70% polar lipids (phospholipids / glycolipids), 27 to 39% soybean oil, 1 to 3% water, and 0.5 to It consists of 3% other components. Among these are polar lipids, which are found in crude lecithin, and It is purified by removing soybean oil, which is a neutral lipid, and can become soy lecithin. The composition of purified lecithin is 22% to 30% phosphatidylcholine. (phosphatidylcholine, hereinafter referred to as PC), 2% to 5% lysophosphatidylcholine (lysophosphatidylcholine, hereafter referred to as LPC), 16% to 22% Phosphatidylethanolamine (PE), 0.5% to 2% lysophosphatidylethanolamine (LPE), 0.5% to 8% Phosphatidic acid (hereinafter referred to as PA), 0.1% to 3% phosphatidylserine (phosphatidylserine), 6% to 15% phosphatidylinositol (phosphatidylinositol), It consists of other components and similar substances. On the other hand, egg yolk lecithin In this case, it contains 73% to 83% phosphatidylcholine (PC), 2% to 5% lysophosphatidylcholine (LPC), and 13% up to 17% phosphatidylethanolamine (PE), 1% to 3% lysophosphatidylethanolamine (LPE), other It consists of components and the like. In this way, lecithin contains a very small amount. Because they contain lysophosphatidylethanolamine (LPE), these lecithins can be used directly. Isolating lysophosphatidylethanolamine (LPE) and making it commercially available is somewhat difficult. It is known that phospholipase D is a phospholipid hydrolysis enzyme. and / or in the presence of phospholipase A, lecithin reacts with ethanolamine. Lysophosphatidylethanolamine (LPE), produced by insertion, can also be used.

[0077] Also, phosphatidylethanolamine (PE) extracted from natural substances by hydrolyzing lysophosphatidylethanolamine (LPE) or phosphatidylcholine (PC) After conversion to phosphatidylethanolamine (PE), it is converted back to lysophosphatidylethanolamine. Lysophosphatidylethanolamine (LPE), obtained by hydrolyzing (LPE), can also be used.

[0078] Hydrogenated lysophosphatidylethanolamine (LPE) can also be used. 14

[0079] Furthermore, there is one fatty acid remaining in the aforementioned lysophosphatidylethanolamine (LPE). at least one unsaturated bond, for example a double bond, must be located at the same place within the chain. a compound to which one or more hydroxyl groups have been added, i.e., hydroxylated. Lysophosphatidylethanolamine (hydroxylated LPE) can also be used.

[0080] The concentration of the lysophospholipid in question is the total phospholipid composition. It can range from 0.1% to 20% by weight. Specifically, the lysophospholipid in question. The concentration can be 0.2% to 10% by weight, more specifically 0.3% to 5% by weight. It could be, and in a specific application example, it could be between 0.5% and 5% by weight, but with that is not limited. A lysophospholipid concentration within the specified content range. When included, the active ingredient, lysophospholipid, can be easily dispersed in the aqueous solution and Suppression of the decomposition of the active ingredient in a water-miscible solvent. The effect may occur. On the other hand, the concentration in question is within the range of content in question. When included below, the amount of the active ingredient in the composition is very small. It may be difficult to demonstrate the intended effect; however, the content range in question... When a lysophospholipid concentration is included, the lysophospholipid dissolves in water. Because it will be present in very large quantities in the miscible solvent, it is not commercially readily available. A problem arises where it is difficult to produce a usable homogeneous mixture. It can come.

[0081] In addition, the phospholipid compound in question has the following Chemical Formula 2: The product shown may contain a sulfosuccinate-based surfactant.

[0082] [Chemical Formula 2]

[0083]

[0084] In Chemical Formula 2 above, R4 and R5 are each hydrogen, sodium or C1 to C18 saturated or unsaturated chain structure or branched structure It could be an alkyl group. However, both R4 and R5 in question could be hydrogen or sodium. It is not. Furthermore, the aforementioned Chemical Formula 2 contains an anionic functional group. It contains a sulfosuccinate group and exists in the form of an alkali salt, for example, its sodium salt. It may be available.

[0085] Specifically, R4 and R5 are independent of each other, from C7 to C8 It can be a saturated or unsaturated chain-structured, or a branched-structured, alkyl group. For example, the chemical formula in question is 2; dioctyl sulfosuccinate (Dioctyl sulfosuccinate, docusate sodium), ammonium dinonyl sulfosuccinate (Ammonium Dinonyl Sulfosuccinate), diamyl sodium sulfosuccinate (Diamyl Sodium Sulfosuccinate), dicapryl sodium sulfosuccinate (Dicapryl Sodium Sulfosuccinate), diethylhexyl sodium sulfosuccinate (Diethylhexyl Sodium Sulfosuccinate), diheptyl sodium sulfosuccinate (Diheptyl Sodium Sulfosuccinate), dihexyl sodium sulfosuccinate (Dihexyl Sodium Sulfosuccinate), Diisobutyl sodium sulfosuccinate, ditridecyl sodium any of the following sulfosuccinate (Ditridecyl Sodium Sulfosuccinate) options may include.

[0086] More specifically, R4 and R5 in the aforementioned Chemical Formula 2, independent of each other, hydrogen, sodium or C8 saturated chain structure or It could be a branched alkyl group. However, both R4 and R5 in question... It is neither hydrogen nor sodium. In a specific application example, the chemical in question... Formula 2 may contain dioctyl sulfosuccinate (docusate sodium), however but not limited to this.

[0087] When the aforementioned sulfosuccinate-based surfactant is included, this The lysophospholipid mentioned above is the active component of the phospholipid composition of the invention, For example, lysophosphatidylethanolamine (LPE) is a homogeneous substance in a water-miscible solvent. to increase the ability to disperse (distribute) in this way and along with It has an effect of suppressing the decomposition of lysophosphatidylethanolamine (LPE). Specifically, the word The subject is the fatty acid portion of the surfactant, a physical bond with LPE, vanillin being an example. It forms a hydrophobic bond via der Waals forces; on the other hand, the hydrophilic part The sulfosuccinate functional group is chemically linked to lysophosphatidylethanolamine (LPE). 16 The bond, for example, forms an ionic bond, such as the aqueous solution of lysophosphatidylethanolamine (LPE). It effectively solves the traditional problem of its easy dissociation in solution. It can suppress other hydrophilic substances. Furthermore, the sulfosuccinate-based surfactant in question... functional groups, for example, surfactants containing the carboxyl group functional group When compared with other substances, lysophosphatidylethanolamine (LPE) has a chemical bond, a for example, having numerous sites where an ionic bond can be formed In terms of quantity and quality, it can form a stronger ionic bond. It has a difference. Therefore, it is different from situations where other types of surfactants are used. In comparison, when sulfosuccinate-based surfactants are used, LPE's The effect of suppressing its breakdown is strengthened and the active ingredient remains for a longer period. The advantage of maintaining its effectiveness can be achieved.

[0088] The content of the sulfosuccinate-based surfactant in question, 0.1% to 20% by weight of the total weight of the phospholipid composition. It can be done. Specifically, the content of the sulfosuccinate-based surfactant in question. 0.2% by weight to 10% by weight, more specifically 0.3% to 5% by weight, a specific In the application example, it can be included by weight from 0.5% to 5%, but is not limited to that. This includes sulfosuccinate-based surfactants within the specified content range. When applied, it increases the dispersion ability of lysophosphatidylethanolamine (LPE) and makes it miscible with water. It has the effect of suppressing its decomposition in the solvent. On the other hand, the content in question... When the subject is included below the range, the sulfosuccinate-based surfactant in the composition Because the amount of the substance is very small, lysophosphatidylethanolamine (LPE) is well-dissipated in aqueous solution. It cannot disperse in a uniform manner, and producing a homogeneous phospholipid composition can be difficult; also It may not be able to suppress the decomposition of lysophosphatidylethanolamine (LPE). Conversely, when the content in question is included above that range, the composition... Because the amount of sulfosuccinate-based surfactant in it will be very high, the active Relatively small amounts of lysophosphatidylethanolamine (LPE) may be included as a component and Therefore, the targeted product effectiveness may not be demonstrated.

[0089] Furthermore, the phospholipid composition of this invention is the anionic surfactant mentioned above. In addition to the sulfosuccinate-based surfactant, which is a substance, there is also a non-ionic (non- It may additionally contain a (ionic) surfactant. The non-ionic surfactant in question Active ingredients: polysorbate 20 (Tween™ 20), polysorbate 40 (Tween™ 40), polysorbate 60 (Tween™ 60), polysorbate 65 (Tween™ 65), polysorbate 80 (Tween™ 80), polysorbate 85 (Tween™ 85), Triton™ N-101, Triton™ X-100, oxtoxinol 40, nonoxynol-9, 17 triethanolamine, triethanolamine polypeptide oleate, polyoxyethylene-660 hydroxystearate (PEG-15, Solutol H15), polyoxyethylene-35-ricinoleate (Cremophor EL™), soy lecithin, poloxamer and There may be at least one or more of these combinations selected from among them. One In this specific application example, the non-ionic surfactant in question is polysorbate. May contain, but is not limited to, Tween™ 80 (Tween™ 80), and the content of this may vary depending on the composition. 0.1% to 5% by weight of total weight, specifically 0.2% to 3% by weight, more Specifically, it may contain 0.5% to 2% by weight. Non-ionic within the stated content range. By adding a surfactant, the effect of the sulfosuccinate-based surfactant is enhanced. without inhibition, the dispersion ability of lysophosphatidylethanolamine (LPE) in aqueous solution is improved. It can also be increased.

[0090] Furthermore, the phospholipid composition of this invention is necessarily miscible with water. The solvent may include, for example, water, and may also include another organic solvent. The matter in question... It may contain organic solvent alcohols. Specifically, the organic solvent in question is ethanol, the best selected from among propanol, butanol, hexanol, octanol and combinations thereof monohydric alcohols containing one or more of the following: 1,3-butanediol, 2,3-butanediol, at least one of the following selected from propanediol, hexanediol, octanediol and combinations thereof. Dihydric alcohols containing one or more of these; trihydric alcohols containing glycerol; and two of these. It may include a mixture of one or more. In a specific application example, the one in question organic solvent; may contain ethanol, glycerol and / or a mixture thereof, but not with It is not limited.

[0091] Furthermore, this invention has a miscible phospholipid composition of C6 to C24 saturated. or an unsaturated chain fatty acid; from among Na, K, Mg, Ca and Al. a fatty acid metal salt containing any selected metal salt; ethanolamine salt, containing any organic salt chosen from among ammonium salt, betaine, and choline salt an organic salt of a fatty acid; or a combination of two or more of these; It may also contain another fatty acid additive.

[0092] The content of the fatty acid additive in question is the phospholipid in question. It can be included by weight at a rate of 1% to 20%, based on the total weight of the composition, detailed It can be included as 5% to 17% by weight, and in a specific application example, 10% to May include, but is not limited to, 15% by weight of fat within the stated ingredient range. When acid additives are present, lysophosphatidylethanolamine (LPE) and / or their derivatives This results in an increased solubility of its derivatives in water-miscible compounds. 18

[0093] In addition, the phospholipid compound in question is lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and two or more of these. It may include further combinations. In one example, in addition to the phospholipid composition in question: Lecithin can be included.

[0094] In addition, the phospholipid compound in question is a chelating agent (metal ion (binder), for example EDTA 4Na (ethylenediaminetetraacetic tetrasodium salt), a fertilizer nutrient components, for example dipotassium phosphate ($K_2HPO_4$) and similar products. It may also include additional elements.

[0095] Furthermore, the phospholipid composition of this invention can be mixed with water in liquid or powder form. It can be a miscible compound. Specifically, the phospholipid composition in question depends on the plant or Applying a treatment to a part of the plant, for example by brushing, spraying, and the like. For ease of methodological implementation, it is in liquid form, a specific application. In the example, it may be preferred in liquid form where a water-miscible solvent is applied, however This is not limited to this and, when addressed in a form suitable for its intended use, It can be prepared and used.

[0096] Phospholipid Compound-Containing Plant Treatment Agent and Treatment With It Method

[0097] The plant treatment agent of this invention may contain a phospholipid compound. A plant or a part of a plant is treated using a plant treatment agent containing a phospholipid compound. Various beneficial effects can be achieved by treating this part. In detail, The preferred plant treatment agent of this invention is the transfer of phospholipid compounds to a plant or to a plant's environment. One to use as a compound in a concentration suitable for treating the part It may be intended for use by diluting it in a solvent.

[0098] The phospholipid compound in question is represented by the following Chemical Formula 1. lysophospholipid, represented by the following Chemical Formula 2. It may contain a sulfosuccinate-based surfactant.

[0099] [Chemical Formula 1] 19

[0100]

[0101] In the above Chemical Formula 1, R1; C5 to C24 are saturated or unsaturated. chain-structured asilox group, C5 to C24 saturated or unsaturated chain-structured alkox group and any of its derivatives; R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structured asilox group and C1 to C5 saturated or It is any of the alkox groups with an unsaturated chain structure, and R3 is hydrogen. It can be any one of the following: choline, ethanolamine, glycerol, inositol, and serine. The subject is the specific content of Chemical Formula 1, as discussed in the phospholipid composition section above. It can be used in the same way as described.

[0102] In addition, the derivative in question contains at least the chemical structure of the R1 in question. a carbon atom in which at least one or more hydroxyl groups are attached to one or more carbon atoms It could be a derivative. In detail, the derivative in question is one or more of the R1 in question. to the highly unsaturated bond, for example, at the location of the double bond, at least one or more The reaction in which a hydroxyl group is attached, i.e., the hydroxylation reaction. It may contain a compound formed through this process, and the lysophospholipid formed in this way, It could be hydroxylated lysophosphatidylethanolamine (hydroxylated LPE). On the other hand, this situation... the chemical structure R3 in question contains at least one or more hydroxyl groups It can also mean binding or substitution. This refers to hydroxylation. (Hydroxylation) reaction, for example, hydrogen peroxide or a similar peroxide a chemical method in which the substance is used; or a lipid oxidation enzyme A biological method performed using lipoxygenase and similar agents; It can be carried out through this means.

[0103] More specifically, the Chemical Formula 1 in question is the following Chemical This can be represented by formula 3.

[0104] [Chemical Formula 3]

[0105] In Chemical Formula 3 above, R1, C5 to C24 are saturated or unsaturated chains. It can be any of the alkyl groups and their derivatives that are selected from among them. Also, the word the derivative in question contains at least one or more components within the chemical structure of R1. It can be a derivative in which at least one or more hydroxyl groups are attached to the carbon atom.

[0106] In a specific application example, Chemical Formula 3 in question, It may contain, but is not limited to, lysophosphatidylethanolamine (LPE).

[0107] Next, the phospholipid compound in question has the following Chemical Formula 2: The product shown may contain a sulfosuccinate-based surfactant.

[0108] [Chemical Formula 2]

[0109]

[0110] In Chemical Formula 2 above, R4 and R5 are each hydrogen, sodium or C1 to C18 saturated or unsaturated chain structure or branched structure It could be an alkyl group; however, both R4 and R5 must be hydrogen or sodium. It is not. Furthermore, the aforementioned Chemical Formula 2 contains an anionic functional group. It contains a sulfosuccinate group and exists in the form of an alkali salt, for example, its sodium salt. 21 It may be available. The specific content of Chemical Formula 2 in question is the phospholipid mentioned above. It can be used in the same way as described in the composition section.

[0111] More specifically, R4 and R5 in the aforementioned Chemical Formula 2, independent of each other, hydrogen, sodium or C8 saturated chain structure or It could be a branched alkyl group. However, neither R4 nor R5 is either hydrogen or sodium. One In the specific application example, the chemical formula in question is dioctyl sulfosuccinate. May contain (dioctyl sulfosuccinate, docusate sodium), but is not limited to.

[0112] Plant treatment agent, active ingredient according to a preferred example of this invention. The phospholipid compound in question has a concentration of 10 to 10,000 in a solvent, such as water. It may have been prepared by dilution within the specified range. The plant treatment agent in question, The subject is lysophospholipid at concentrations of 1 to 5,000 ppm (w / w), specifically 1 to 3,000 ppm (w / w), more specifically, inclusion at a concentration of 1 to 1,000 ppm (w / w). It may include, but is not limited to.

[0113] A part of the plant in question, such as fruit, leaf, flower, root, stem and There may be at least one or more selected from among the lumps. The "life" in question... The term "life cycle" refers to the life cycle of a plant or plant part, both before and after harvest. It can encompass both post-harvest stages.

[0114] The plant treatment agent of this invention; in a plant or part of a plant Improving characteristics and overall health status, stimulating growth, It can have an effect on improving quality of life, such as delaying aging. The fruits in question are: apples, pears, tomatoes, peaches, kiwis, plums, cherries, grapes, cranberries, and wild greens. It could be at least one of them, but not limited to, myrtle and strawberry.

[0115] Based on these properties, this invention can be used as a plant treatment agent in various ways. By being applied, it can assist the plant or agricultural industry. Specifically, this invention... plant treatment agent; a substance that improves the quality of a plant or part of a plant, such as color, flavor, at least one or more of the following are selected from firmness, sugar content, fruit cracking and aroma It can improve more than that. In addition, this invention, as a plant treatment agent, can improve a plant's... or it may have an effect that delays the aging of a part of the plant, and thus the plant's or it may have an effect that extends the storage time or shelf life of a part of the plant. 22

[0116] Furthermore, the plant treatment agent of this invention is used to treat a plant or part of a plant. by stimulating their growth, the diameter and width of their fruits, vegetables, roots and / or tubers, It can have an enhancing effect on at least one or more of the selected weights and numbers.

[0117] Furthermore, this invention's plant treatment agent can treat a plant or a part of a plant It may exhibit a protective effect against biotic or abiotic stress. Specifically, the word The topic is biotic stress; pathogens (e.g., fungi, protozoa, bacteria, viruses, etc.), insects, nematodes, snails, mites, weeds, humans, non-human animals, and biotics containing at least one or more of these combinations This may include physical damage resulting from various causes. Abiotic stress in question includes freezing, wind, hail, floods, drought, heat, and pesticides (e.g., plant growth regulators, (insecticides, fungicides, herbicides, spreaders-adhesives, fertilizers, etc.) at least one of these or it may include physical damage resulting from more abiotic causes.

[0118] In addition, the plant treatment agent of this invention may be used to treat a plant or a part of a plant. part of it is starch, protein, lipid and a combination of two or more of these. It can have an effect that enhances the synthesis of selected components.

[0119] Below, in order to describe this invention more specifically, according to this invention The preferred experimental examples are described in more detail by referring to the attached figures. will be explained. However, this invention is not limited to the application examples described here. It can also be embodied in other forms.

[0120] Application Examples 1 to 3: Improved Long-Term Storage Stability Production of a Phospholipid Compound with Formulation

[0121] Lysophospholipid (LPE), represented by Chemical Formula 1 mentioned above, sulfosuccinate-based surfactant shown with Chemical Formula 2 mentioned above substance, organic solvent (ethanol), water, non-ionic surfactant, glycerol, oil acids, lecithin and $K_2HPO_4$; to be included in the composition ratios in Table 1 below. A phospholipid compound was produced by mixing the ingredients in this manner at room temperature.

[0122] Comparison Example: Conventional Lysophospholipid-Containing Phospholipid Composition

[0123] Sulfosuccinate based, represented by Chemical Formula 2 mentioned above. Except for the omission of surfactants, Application Examples 1 through 3 are the same. A phospholipid compound was produced using this method. 23

[0124] This invention corresponds to Application Examples 1 to 3 and the Comparison Example. The composition and contents of the incoming phospholipid compounds are summarized in Table 1 below.

[0125] [Table 1] Article name (unit: weight %) To compare example Application example 1 Application example 2 Application example 3 LPE 1 1 1 1 Ethanol 10 10 10 10 Water 45 44 42 40 Tween 80 1 1 1 1 Glycerol 20 20 20 20 EDTA 4Na 2.5 2.5 2.5 2.5 Fatty acids 11 11 11 11 Lecithin 6 6 6 6 K₂HPO₄ 3.5 3.5 3.5 3.5 Surfactant S 0 1 3 5

[0126] In Table 1 above, Surfactant S stands for sulfosuccinate surfactant. It refers to the article.

[0127] Sample Test 1: Storage Stability for Phospholipid Composition Evaluation

[0128] Figure 1 shows a long-term storage example of an application of this invention. types of water-miscible phospholipid compounds with improved stability formulations These are the results of changes in residue levels during storage.

[0129] Referring to Figure 1, Application Example 2 of this invention and For the phospholipid compositions in the Comparison Sample, the amount of LPE residue in the composition. by measuring the ratio, it remains as an active ingredient even in long-term storage and preservation situations. It has been confirmed that LPE remains intact without decomposition. In the application example of this invention... formulation of the composition using a miscible phospholipid composition with water To evaluate storage stability; 6 months of storage at room temperature. As an equivalent accelerated test condition, the composition is at constant temperature at 54 °C. It was stored in its room for a maximum of 10 weeks. As a result, Application Example 2 24 and according to Application Example 3, phospholipid containing sulfosuccinate surfactant its composition shows 94% or more LPE compared to baseline, even after 5 weeks. It has been confirmed that it ensures the residual rate, and this result is consistent with the Comparison. Compared to its counterpart, it has an extremely superior storage stability. It can be concluded that, in this way, the phospholipid composition in aqueous solution form of this invention, It has been confirmed that long-term storage stability improves even in aqueous solution.

[0130] Therefore, in the test examples which will be explained below, the aforementioned The phospholipid composition corresponding to the composition of Application Example 2 was chosen as the test group. It can be used in this way.

[0131] Sample Test 2: Against Strawberry Powdery Mildew Disease (Sphaerotheca aphanis) Testing the Control Effect

[0132] Red Pearl variety of strawberry was used as a test product under greenhouse cultivation. and its target disease is control against powdery mildew disease (Sphaerotheca aphanis). To test its effectiveness, the leaves were treated three times at 7-day intervals at the onset of the disease. (Treatment was applied to the stem and leaves). Control group 1 was untreated. Group 1, control group 2, received 4,000 liters of triflumizol wettable powder (30% active ingredient). A multi-layered diluted solution was used, and the phospholipid composition of this invention was used as the test group. (Application Example 2, active ingredient content 1%) A solution diluted 1,000 times was used. The layout of the test plots and the required space were determined according to a randomized block design. repeatedly; application number 3 times, repetition number 3 times, total 9 parcels and required area. The study was conducted under conditions of 180 m². As an inspection method, the entire test plot was examined. The percentage of diseased fruits was investigated. The test results were compared with the final product. This data is from the 7th day after the application and is shown in Table 2 below; consequently When the phospholipid composition of this invention is applied, it controls strawberry powdery mildew disease. It can be confirmed that its effect exists.

[0133] [Table 2] Treatment group Disease rate: Statistical difference (DMRT) Protection rate (%) 1st repetition 2nd repetition 3rd repetition Average No action taken (Comparison group 1) 24.4 19.8 21.8 22.0 c - Triflumizole wettable powder (Comparison group 2) 2.5 3.5 4.0 3.3 a 85.0 Phospholipid composition (Application example 2) 7.8 8.4 8.7 8.3 b 62.3

[0134] Test Sample 3: Kiwi Cancer Disease (Pseudomonas syringae pv. Testing the Control Effect with a Counter-Drug (Actinidiae biovar)

[0135] Jecy Gold variety of kiwi as a test product under open field cultivation. It has been used, and the target disease is cancer (Pseudomonas syringae pv. To test the control effect against Actinidiae biovar, 7 patients were treated at the onset of the disease. The leaves were applied three times at intervals of one day (to the stem and leaves). Control group 1 The group not treated was the control group 2, which received oxytetracycline streptomycin sulfate. a 1,500-fold diluted solution of the wettable powder (active ingredient content 20.3% by weight). used and as a test group the phospholipid composition of this invention (Application Example 2, A solution containing the active ingredient (1% by weight) diluted 500 times was used in the test. The layout of the plots and the required number of trees were determined according to a fully randomized experimental design. repeatedly; application number 3 times, repetition number 3 times, total 9 plots, per plot The study was conducted under conditions requiring 1 tree and 9 trees. As a method, 200 leaves in the test plot 10 days after the final drug application. The number of diseased leaves inside was investigated.

[0136] Figure 2 shows the phospholipid composition of kiwi according to an application example of this invention. This is a result from testing the control effect against cancer.

[0137] Test results are from the 7th day after final product application and are as follows: As shown in Table 3 and Figure 2, the phospholipid composition of this invention is consequently... 26 When applied, kiwi has a control effect against cancer. This can be verified.

[0138] [Table 3] Treatment group Disease rate Statistical difference (DMRT) Protection Rate (%) 1st repeat 2nd repeat 3rd repeat Average No treatment applied (Comparison group 1) 29.5 32.5 31.0 31.0 c - Oxytetracycline, streptomycin sulfate wettable powder (Comparison group 2) 6.5 10.5 6.0 7.7 a 75.2 Phospholipid composition (Application) Example 2) 14.5 12.5 18.5 15.2 b 51.0

[0139] Sample Test 4: Verification Test of Wheat Yield and Protein Increase

[0140] Forefront variety wheat as a test crop under open field cultivation The effect of using this method on increasing the yield and protein content of wheat was tested. Control group The group that was not treated as such was used and this invention's phospholipid was used as the test group. its composition (Application Example 2, active ingredient content 1% by weight) diluted 1,000 times The solution was used. The timing and method of application was flowering (anthesis). The application was carried out on the 5th day following. The layout of the test plots and the necessary procedures were determined. Area / number of trees, applied using a completely randomized experimental design with 3 replications; application Number of repetitions: 2 times, number of repetitions: 1 time, number of test plots: 3 plots, area per plot: 1 ha. The study was conducted in a required area of ​​6 hectares. As a research method, bushel analysis was used for yield. The number of bushels was investigated (unit: ton / ha) and a wholegrain analyzer was used for protein. Analysis was performed using an analyzer (unit: %). Test results are shown in Table 4 and Table 2 below. As shown in Figure 5, when this invention is compared with the control group condition, the test In this group, wheat yield increased by approximately 6% to 11%, and wheat protein content also increased. It has increased by approximately 4% to 9%. Therefore, the phospholipid composition of this invention When applied, it has an effect on increasing wheat yield and protein content. This can be confirmed. 27

[0141] [Table 4] Application group Yield (tons / ha) Experiment 1 Experiment 2 Experiment 3 3.6 2.8 2.9 (without application) Application example 2 3.9 3.1 3.1 Increase rate: 8.3% 10.7% 6.8%

[0142] [Table 5] Treatment group Protein content (%) Experiment 1 Experiment 2 Experiment 3 Untreated 12.7 12.7 14.2 Application example 2 13.6 13.8 14.7 Increase rate 7% 9% 4%

[0143] Test Sample 5: Effect of Suppressing Frost Damage (Cold Damage) in Apples Testing

[0144] Frost damage to fruits; damage occurring during and around the flowering period. In this case, the stigma and ovule darken, and in severe cases, the flower. drying out without opening or, even if it flowers, failure to pollinate the fruit The bending of the stem indicates that the fruit will develop an abnormal shape and fall off prematurely. Using the Hongro apple variety as a test product under open field cultivation, the apple's... The effect of suppressing frost damage was tested. The untreated group served as a control group. The group was used and the phospholipid composition of this invention (Application Example) was used as a test group. 2. A solution containing the active ingredient (1% by weight) diluted 1,000 times was used. Application time and method: twice, 5 days apart, to the leaves (stem and leaves). The experiment was conducted. The layout of the test plots and the required number of trees were entirely random. According to the experimental design, with 3 repetitions; number of applications 2 times, number of repetitions 3 times, Total number of plots: 6 plots, number of trees per plot: 1 tree, required number of trees: 6 The study was conducted under tree conditions. The bud measurement method was used to determine the flowering rate. The number of central flowers that bloom per head was investigated, and the pollination rate was measured using the number of blooming centers. The number of pollinated seeds within the flowers was investigated. 28

[0145] Figure 3 shows the phospholipid composition according to an application example of this invention. Testing the suppressive effect against frost damage in Hongro apple varieties using this method. This is a result of its development; Figure 4 shows an example of an application of this invention in phospholipid. Showing the change in peel color of Hongro variety apples before harvest using a composition. It is a result.

[0146] Test results are shown in Table 6, Table 7, Figure 3 and Figure 4 below. Accordingly, compared to the control group condition of this invention, flowering occurred in the test group. The rate was approximately 1.8 times higher on average, and the fertilization rate was approximately... It showed a result that was 1.4 times higher. Therefore, the phospholipid composition of this invention When applied, it has a suppressive effect against frost damage in apples. This can be verified.

[0147] [Table 6] Application group Flowering rate Statistical difference (DMRT) 1st repetition 2nd repetition 3rd repetition Average No intervention (control group) 10 5 9 8.0 a Application example 2 16 12 15 14.3 b

[0148] [Table 7] Treatment group Fertilization rate Statistical difference (DMRT) 1st repetition 2nd repetition 3rd repetition Average a Untreated (control group) 38 32 43 37.6 a Application example 2 54 47 62 54.3 b

[0149] Sample Test 6: Evaluation of Encouraging Coloration in Apples Test

[0150] The “Picnic” variety of apple was used as the experimental plant and the experimental site was At the National Horticultural and Specialty Products Science Institute, affiliated with the Rural Development Agency A test to accelerate apple coloring (stage 1) has been carried out. This invention... Phospholipid composition (shown as LPE), 0 mg · L-1 (untreated), 20 mg · L-1 Spraying of (Application Example 2(1)) and 40 mg · L-1 (Application Example 2(2)) 29 (spray) application was carried out, and the application method was 1. 6 weeks before harvest. Application 1: 2nd application 4 weeks before harvest, and 3rd application 2 weeks before harvest. This was carried out. The apple's coloration was measured using a Hunter colorimeter. This was achieved using the Hunter “a” value, which indicates the degree of redness of the fruit peel; The higher the value of 'a', the darker the red color, and This means the apple's coloring is accelerating.

[0151] Figure 5 shows the phospholipid composition according to an application example of this invention. Testing the use of methods to promote coloring in Picnic variety apples. It is a result.

[0152] As shown in Table 8 and Figure 5 below, the Picnic variety of apple When the change in shell color was measured, it was treated with the phospholipid composition belonging to this invention. In the experimental group, the reddest part of the fruit (shown as Red part) and the least In both cases, the red part (shown as the non-red part) has a high value for 'a'. It was measured; especially under the condition of 20 mg · L-1 (Application Example 2(1)) the value of a was at most It has been determined that it is high. Therefore, treatment with the phospholipid composition of this invention... It can be confirmed that apple coloring can be accelerated in this way.

[0153] [Table 8] Phospholipid Composition CONCENTRATION (MG · L-1) RED PART NON-RED PART L* a* b* L* a* b* 0 (no transaction applied) 50.45 26.98 23.21 76.20 -3.10 39.96 (Application Example 2(1)) 39.97 35.25 17.86 67.88 7.55 34.85 40 (Application Example 2 40.91 34.63 18.15 73.76 -0.47 38.33

[0154] Then, using the Fuji variety as the experimental plant and as the experimental site An apple farm in Cheongsong was selected to accelerate the coloring of the apples. Testing (stage 2) for this invention was carried out. The phospholipid composition of this invention is 0 mg · L-1 (process (not applied) and 20 mg · L-1 (Application Example 2(1)) application concentrations It was applied by spraying; the application method was 1. 5 weeks before harvest. The first application and the second application were carried out 3 weeks before harvest.

[0155] As shown in Table 9 below, apples for the Fuji variety As a result of measuring the change in shell color, the treated group was compared with the control group. Compared to the untreated parcel, the phospholipid composition of this invention was tested. in the group (indicated as LPE treated plot), the reddest part of the fruit (Indicated as the red part) and the least reddish part (indicated as the non-red part) (as stated) in both sections, the value of "a" was measured as high. This can be verified. Therefore, the application of this invention to phospholipid composition can be proven. It is possible to encourage apple coloring by doing this. This can be verified.

[0156] [Table 9] Untreated group LPE treated group Non-red Red Non-red Red L* 61.3±2.4 45.6±2.4 57.1±4.4 40.1±1.3 a* 8.0±4.9 26.7±3.3 17.2±6.5 28.8±2.8 b* 28.8±4.0 17.5±2.3 25.6±3.9 13.9±0.7

[0157] Sample Test 7: Encouraging Coloration in Grapes Assessment Test

[0158] Campbell Early (Vitis labrusca L.) variety was used as the experimental plant and The experiment was conducted at a grape farm in Siheung City to color the grapes. Tests aimed at accelerating the process have been carried out. The phospholipid compound (LPE) of this invention (shown as), 0 mg · L-1 (untreated), 20 mg · L-1 (Application Example 2(1)) and spray at concentrations of 40 mg · L-1 (Application Example 2(2)) The application was carried out, and the application method was the first application 4 weeks before harvest. A second application was carried out 2 weeks before harvest. Regarding the coloring of the grapes. Measurements were made by visually observing the color change (apparent difference) and using Hunter colorimeter. Using Hunter L, a and b values, which indicate the degree of redness of the shell color. This was achieved by measuring; the higher the 'a' value above, the darker it is. red, the higher the b value, the darker the blue, and the lower the L value... It means it's a very dark color, almost black. 31

[0159] Figure 6 shows the phospholipid composition according to an application example of this invention. Testing the use of materials to promote coloring in Campbell Early grape varieties. It is a result of being done.

[0160] As shown in Table 10 and Figure 6 below, Campbell Early By measuring the change in grape skin color according to the variety, treatment Compared to the untreated parcel, the phospholipid composition of this invention was tested. The color observed with the naked eye in all the fruits in the group has become more vibrant. It has been confirmed that it arrived; a dark mark was obtained by measuring the greatest decrease in the L value. It has been confirmed that the displayed colors are red and blue, with a decrease in both the a and b values. It has been confirmed that the colors are reduced. Therefore, the phospholipid composition of this discovery... It is possible to encourage grape coloring through the application of this method. This can be confirmed.

[0161] [Table 10] Phospholipid composition Concentration (mg · L-1) L* a* b* 0 (untreated) 24.4 ±2.3 3.6 ±1.5 -3.0 ±1.2 (Application Example 2(1)) 18.6 ±3.5 -0.8 ±1.1 -2.6 ±1.1 40 (Application Example 2 21.8 ±3.4 0.4 ±1.7 -2.4 ±1.3 Average 21.6 1.07 -2.7

[0162] Test Sample 8: Phospholipid Composition in Apples According to Formulation Coloration Induction Test

[0163] The phospholipid composition of this invention, which has improved storage stability, Apple coloring similar to existing formulations even 7 days after production. a test evaluation to determine whether it demonstrates effectiveness The experiment was carried out. The "Picnic" variety of apple was used as the experimental plant, and the experiment was conducted in Rural Area. Development Agency National Horticultural and Specialty Products Science Institute apple coloring This was performed as a stimulus test. The situation where the phospholipid compound was not applied. (unapplied), 7 days after production of the phospholipid composition in the comparison sample. the formulation obtained after passing (comparison example (7 days past)) (as stated) with the application at a rate of 20 mg · L-1, this invention's second application. According to the example, the formulation obtained 7 days after the phospholipid composition was prepared. 32 (Application Example 2 (after 7 days) is shown) Spray at a rate of 20 mg · L-1. The application was done via foliar application; the application method was 6 weeks before harvest. The first application was carried out, and the second application was done 4 weeks before harvest. The apple... Measurements regarding coloration were taken using a Hunter colorimeter on the fruit peel. The Hunter “a” value, which indicates the degree of redness, was measured; what is this “a” value? The higher the reading, the darker the red color, and the more the apple is colored. It means it's accelerating.

[0164] Figure 7 shows an application example of this invention after 7 days of production. Color enhancement in Picnic variety apples using a previously developed phospholipid composition. It is a result of testing its effectiveness.

[0165] As shown in Table 11 and Figure 7 below, the phospholipid composition According to the formulation, the change in apple peel color for the Picnic variety Measurements showed that, when compared to the untreated plot, the fruit yield was highest. the red part (referred to as "Red part") and the least red part (Non-red) In both parts, including (as specified as part), the value of "a" is high. measured and it is possible to encourage apple coloring This can be verified.

[0166] [Table 11] LPE Red part Non-red part L* a* b* L* a* b* No transaction applied 50.45a 16.98a 23.21a 76.20a -5.10a 39.96a Example of comparison (7 days later) 39.97b 25.25b 17.86b 71.88b 2.55b 34.85b Application example 1 (7 days later) 40.91b 24.63b 18.15b 69.76bc 4.47b 32.33b

[0167] Test Sample 9: Phospholipid Composition in Apples According to Time Flow Coloration Induction Test

[0168] According to this invention, the phospholipid composition depends on the post-production storage time. whether or not it exhibits a change in the LPE content, which is the active ingredient. It has been checked. 33

[0169] Referring to Table 12 below, the phospholipid composition product As a result of measuring the change in the remaining LPE content, application example in this case, both if 7 days have passed since production and if 12 months have passed. In this case, a high level of content when compared to the comparison example. It can be verified that it provides protection.

[0170] [Table 12] LPE 1% by weight SL formulation LPE content 7 days after production 12 months after production Comparison example: 0.99 0.35 Application example 2 1.02 0.93

[0171] Figure 8 shows an application example of this invention 1 year after its production. Using the phospholipid composition from the past, the color of the Picnic variety apple peel was determined. It is a result related to measuring change.

[0172] Also, referring to Table 13 and Figure 8 below, If the product is used more than one year after its production date, Picnic By measuring the change in apple peel color according to the variety; When compared to the untreated plot and the comparison sample, the fruit yield was highest. the red part (referred to as "Red part") and the least red part (Non-red) In both parts, including (as specified as part), the value of "a" is high. measured and it is possible to encourage apple coloring This can be verified. Between the untreated parcel and the comparison example... It can be confirmed that almost no difference in coloration is observed. 34

[0173] [Table 13] Phospholipid composition: Red part, Non-red part L* a* b* L* a* b* No transaction applied 49.45a 16.88a 27.81a 71.20a -4.01a 33.06a Example of comparison (1 year later) 47.97b 17.24a 25.44ab 70.88a -1.55ab 31.85ab Application example 2 (1 year later) 38.91b 26.55b 18.15c 49.76b 7.32c 22.68c

[0174] Test Sample 10: Phospholipid Composition in Grapes According to Formulation Coloration Induction Test

[0175] The phospholipid composition of this invention, which has improved storage stability, Even 7 days after production, grape yields are similar to those obtained with current formulations. a test to determine whether or not it shows coloring activity The evaluation was carried out. Campbell Early (Vitis labrusca L.) was used as the experimental plant. The variety was used and the grape farm in Siheung city was used as the experimental site. A test to accelerate coloration was performed. No phospholipid compound was applied. case (not applied), 7 from the production of the phospholipid composition in the comparison sample. the formulation obtained after 7 days (comparison example (7 days past)) (as stated) the situation with the application of 20 mg · L-1, and the phospholipid of this invention. The formulation obtained 7 days after the preparation of the composition (Application Example 2) (As shown after 7 days) foliar spraying at a rate of 20 mg · L-1 The application was carried out, and the application method was the first application 4 weeks before harvest. The second application was carried out 2 weeks before harvest. The coloration of the grapes, It was checked using a visual inspection method.

[0176] Figure 9 shows an application example of this invention after 7 days of production. using the phospholipid composition from the past in Campbell Early grape varieties This is a result from testing the effectiveness of coloration stimulation.

[0177] Referring to Figure 9, according to the phospholipid composition formulation For the Campbell Early variety, changes in grape skin color can be observed with the naked eye. 35 As a result of observation, the comparison example and Application Example 2 The difference between the formulations was almost indistinguishable; however, the untreated Comparison Example and Application Example 2 applied when compared with the parcel. It can be verified that grape coloring is visually stimulated in the plots.

[0178] Also, as seen in Table 14 below, the phospholipid composition Campbell Early was prepared using the formulation obtained 7 days after preparation. The anthocyanin content in the grapes was measured. Anthocyanin in the fruit peel. The contents are 10 pieces of the peel taken from the equatorial plane of the fruit using a cork borer (5 mm). Sections were taken and these sections were immersed in a 0.1 N HCl-100% EtOH (15:85, V / V) solution. After being left in a dark and cool environment for 24 hours, the spectrophotometer (UV-2501 PC, Absorbance was measured at 535 nm by Shimadzu, Japan) and Fuleki & Francis (1968a, The total anthocyanin content value calculated according to method 1968b) was used. Therefore, 7 days after its production, in the LPE formulation (Application Example 2), Regardless of the variety, LPE treatment accelerates the coloring of grapes and increases anthocyanin levels. It can be observed that the content has increased.

[0179] [Table 14] Treated group Anthocyanin (mg / L) Statistical Significance (DMRT) Untreated group 1.5 ± 0.5 a Comparison example (after 7 days) 2.7 ± 0.3 b Application example 2 (after 7 days) 2.9 ± 0.2 b

[0180] Experimental Example 11: Effect of phospholipid composition on grape coloration over time acceleration test

[0181] Post-production storage of the phospholipid composition prepared according to this invention Checking whether there is a change in the content of the active substance LPE depending on the duration. It has been done.

[0182] See Table 15 below, the storage time of the phospholipid composition Depending on the context, changes in the remaining LPE content in the product were measured; according to the results, In the application example, 7 days after production and 12 months later, compared to the comparison example. It has been observed that a relatively high level of content has been maintained. 36

[0183] [Table 15] LPE 1 weight% SL formulation LPE content 7 days after production 12 months after production Comparison example: 0.99 0.35 Application example 2 1.02 0.93

[0184] Figure 10 shows an example of an application of this invention 1 year after its production. the phospholipid composition used in the past and depending on the storage time of the composition A study on observing changes in the skin color of Campbell Early grapes. This is the result.

[0185] Referring to Figure 10, it is 1 year past its production. Grape skin treatment using phospholipid composition for Campbell Early variety. As a result of observing the change in color with the naked eye; untreated plot There is almost no difference in coloration between this and the comparison sample. not observed; however, with the untreated parcel or comparison sample In comparison, for Example 2, the color of the grapes darkened and the coloring improved. It can be confirmed that it was encouraged.

[0186] Also, application example when referring to Table 16 below. in this case the anthocyanin concentration of Campbell Early grape variety treatment a high content when compared with the parcel not included or comparison sample It can be confirmed that it has maintained its level.

[0187] [Table 16] Treatment group Anthocyanin (mg / L) Statistical difference (DMRT) Untreated 2.5 ± 0.5 a Comparison example (after 1 year) 3.1 ± 0.3 ab Application example 2 (after 1 year) 4.0 ± 0.1 c

[0188] On the other hand, this invention described in these explanations and drawings Application examples are provided as specific examples only to facilitate understanding. This has been presented and is not intended to limit the scope of this invention. Here In addition to the application examples described, there are other examples based on the technical idea of ​​this invention. general knowledge in the technical field to which this invention belongs, and the application of variations thereof It is clear to a person who owns it.

Claims

37 REQUESTS 1. A lysophospholipid represented by the chemical formula 1 below. and, a sulfosuccinate-based product shown with Chemical Formula 2 below. A phospholipid composition containing a surfactant: [Chemical Formula 1] In Chemical Formula 1 above, R1; C5 to C24 are saturated or unsaturated chains. structured asilox group, C5 to C24 saturated or unsaturated chain structured alcox group and It is any one of the derivatives chosen from among them, R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structured acylox. selected from group and C1 to C5 alcove groups with saturated or unsaturated chain structures is anyone and, R3; any one of the following selected from hydrogen, choline, ethanolamine, glycerol, inositol and serine. one of them, [Chemical Formula 2] 38 In Chemical Formula 2 above, R4 and R5 each represent hydrogen independently. sodium or saturated or unsaturated, chain-type or branched alkyl groups between C1 and C18. They are groups; however, it is possible for both R4 and R5 to be hydrogen or sodium. It is not.

2. According to Claim 1, the derivative in question has the chemical structure of R1. at least one or more hydroxyl groups on at least one or more carbon atoms a phospholipid compound to which it is linked, which is a derivative.

3. According to Claim 1, the phospholipid compound in question is available in liquid or powder form. A phospholipid compound that is miscible with water.

4. According to Claim 1, the concentration of the lysophospholipid in question must be such that... Phospholipids that constitute 0.1% to 20% by weight of the total weight of the phospholipid composition. composition.

5. According to Claim 1, the content of the sulfosuccinate-based surfactant in question, 0.1% to 20% by weight of the total weight of the phospholipid composition in question. the phospholipid composition in which it is made.

6. According to Claim 1, the phospholipid compound in question; C6 to C24 are fatty acids with a saturated or unsaturated chain structure; An oil containing any metal salt chosen from among Na, K, Mg, Ca, and Al. acidic metal salt; Any one of the following can be selected: ethanolamine salt, ammonium salt, betaine, or choline salt. an organic salt of a fatty acid containing organic salt; or a combination of two or more of these; a fatty acid additive containing its composition, including the phospholipid component it contains.

7. According to Claim 6, the content of the fatty acid additive in question must be such that... 1% to 20% by weight of phospholipid composition, based on total weight. Phospholipid composition. 39 8. According to Claim 1, the phospholipid compound in question; ethanol, propanol, butanol, hexanol, octanol and combinations thereof monohydric alcohols containing at least one or more of the selected types; 1,3-butanediol, 2,3-butanediol, propanediol, hexanediol, octanediol and their derivatives Dihydric alcohols containing at least one or more of the following combinations; Trihydric alcohol containing glycerol; and an organic solvent containing a mixture of two or more of these. its phospholipid composition.

9. According to Claim 1, the phospholipid compound in question is lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and two or more of these. the combination of its phospholipid composition.

10. A plant or part of a plant containing a phospholipid compound. By treating the plant or part of the plant with a treatment agent, the health of the plant or part of the plant in question is improved. the step of changing its condition, growth or life cycle a method that includes; The phospholipid compound in question is represented by the following Chemical Formula 1. lysophospholipid, represented by the following Chemical Formula 2. A method involving a sulfosuccinate-based surfactant: [Chemical Formula 1] 40 In Chemical Formula 1 above, R1; C5 to C24 are saturated or unsaturated chains. structured asilox group, C5 to C24 saturated or unsaturated chain structured alcox group and It is any one of the derivatives chosen from among them, R2; hydrogen, hydroxyl group, C1 to C5 saturated or unsaturated chain structured acylox. selected from group and C1 to C5 alcove groups with saturated or unsaturated chain structures is anyone and R3; any one of the following selected from hydrogen, choline, ethanolamine, glycerol, inositol and serine. one of them, [Chemical Formula 2] In Chemical Formula 2 above, R4 and R5 are each hydrogen, independently. sodium or C1 to C18 saturated or unsaturated chain structure or branched structure It is an alkyl group; however, both R4 and R5 are hydrogen or sodium. It is not.

11. According to claim 10, the derivative in question has the chemical structure of R1. at least one or more hydroxyl groups on at least one or more carbon atoms The method by which it is connected is a derivative.

12. According to Claim 10, the plant treatment agent in question is related to the lysophospholipid in question. A method in which an aqueous solution is included at a concentration of 1 to 5,000 ppm (w / w).

13. According to Claim 10, a part of the plant in question; fruit, leaf, flower, root, A method in which at least one or more of the stem and tuber are selected. 41 14. According to Claim 10, the plant treatment agent in question must be in liquid or powder form. a method in which the compound is miscible with water in form.

15. According to Claim 10, the plant treatment agent in question is lecithin, hydroxylated lecithin, acetylated lecithin, enzyme-treated lecithin, and combinations thereof. a method that includes at least one or more of the selected items.

16. According to Claim 10, the method in question involves the processing of a plant or part of a plant. A method aimed at improving its quality.

17. According to Claim 16, the quality in question includes color, flavor, firmness, sugar content, fruit A method that involves at least one or more of the following: cracking and odor.

18. According to Claim 10, the method in question involves the processing of a plant or part of a plant. A method aimed at delaying aging.

19. According to Claim 18, delaying the aging in question is possible if a plant or a part of the plant is intended to extend its storage time or shelf life method.

20. According to Claim 10, the method in question involves the processing of a plant or part of a plant. by stimulating their growth, the diameter and width of their fruits, vegetables, roots and / or tubers, increasing at least one or more of the items selected from among their weight and number the method used.

21. According to Claim 10, the method in question involves biotically damaging a plant or part of a plant. or methods aimed at protecting against abiotic stress. 42 22. According to Claim 21, the abiotic stress in question includes freezing, wind, hail, flood, drought, among temperature and pesticides, at least one or more abiotic causes the method involves the resulting physical damage.

23. According to Claim 22, the pesticides in question are plant growth regulators, insecticides (insecticides), fungicides (fungus killers), herbicides (weed killers), containing at least one or more selected from among spreaders-adhesives and fertilizers method.

24. According to Claim 21, the biotic stress in question is caused by pathogens, insects, nematodes, among snails, mites, weeds, humans and non-human animals physical damage resulting from at least one or more biotic causes the method involved.

25. According to Claim 24, the pathogens in question are fungi, protozoa, bacteria, and A method that involves at least one or more viruses.

26. According to Claim 10, the method in question involves the processing of a plant or part of a plant. from among starch, protein, lipid and two or more of these A method aimed at increasing the synthesis of selected components.

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