Crispr repair in vitro screen (CRIS) off-target assessment

Abstract

Disclosed herein are methods to assess off-target sites resulting from CRISPR Cas nuclease systems wherein the methods require fewer cells than conventional strategies and can provide robust signal reads over noise. Also provided are methods of identifying Cas nuclease off-target cut-sites from the resulting reads using a computational pipeline including an artificial neural network model trained on known on-target and off-target cut-sites for the Cas nuclease.