Monitoring time-varying fluorescence emitted from an exogenous fluorescence agent

Abstract

Method is presented for monitoring time-varying fluorescence. The method includes providing a measurement data set, transforming each Flr signal of each measurement data entry of the measurement data set to an agent intrinsic fluorescent (IF) signal, monitoring the agent IF signal for each measurement data entry within the measurement data set, whereby fluorescence emission of the agent is determined with reduced sensitivity to the time-varying optical properties of the medium.