Methods for manufacturing nerve cells that induce TDP-43 aggregation in the cytoplasm and their applications

Abstract

The present invention addresses the following: a method for manufacturing nerve cells inducible to aggregate TDP-43 in the cytoplasm; a method for inducing TDP-43 aggregation in the cytoplasm of nerve cells without inducing cell death; cell aggregates obtained by the above-described method for manufacturing nerve cells; and methods for evaluating test substances using the above-described method and for screening drugs for the prevention and / or treatment of neurodegenerative diseases. According to the present invention, a method for manufacturing nerve cells inducible to aggregate TDP-43 in the cytoplasm is provided, comprising: step A, culturing nerve cells differentiated from human pluripotent stem cells in a culture medium containing a concentration of protein synthesis inhibitors that do not induce cell death.