Truncated chromatin open element sequences, their combinations and applications

Truncated UCOE sequences integrated with PiggyBac transposon systems address inefficiencies in cell line construction by improving transfection efficiency and stability, shortening the construction cycle, and maintaining high yield for antibody production.

TW202631993APending Publication Date: 2026-08-01SHANGHAI QILU PHARMACEUTICAL RESEARCH & DEVELOPMENT CENTRE LTD
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Patent Information

Application Number
TW114139082
Authority / Receiving Office
TW · TW
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-10-10
Filing Date
2025-10-09
Publication Date
2026-08-01

AI Technical Summary

Technical Problem

Current methods for constructing stable cell lines for antibody production face low integration efficiency, labor-intensive screening, poor target gene stability, and slow cell line construction times, particularly when using unopened chromatin elements (UCOEs) in CHO cells, with issues like low transfection efficiency and long construction cycles.

Method used

Integration of truncated chromatin open elements (UCOEs) with PiggyBac transposon systems, utilizing specific ITR sequences for precise genome insertion, enabling rapid bulkpool screening and significantly shortening construction cycles while maintaining high protein yield.

Benefits of technology

The combined use of truncated UCOE sequences with ITRs enhances transfection efficiency, stabilizes target gene expression, and accelerates cell line development by 4-5 weeks, facilitating rapid industrial-scale antibody production.

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Abstract

This disclosure relates to a truncated chromatin open element and combinations thereof, as well as an expression system for eukaryotic cells containing the chromatin open element or combinations thereof, and its applications. The chromatin open element is a truncated sequence of two chromatin open elements from different sources, which can be used alone or in combination. This chromatin open element significantly shortens the effective sequence, thus facilitating the integration of vectors containing the element or combinations thereof with other elements, such as ITR integration. Semi-site-specific integration is achieved in the presence of PiggyBac transposase, followed by a rapid bulkpool screening process, thereby shortening the protein production and cell line construction cycle by approximately 4-5 weeks while maintaining comparable yield.
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