An in vitro expansion method of cytokine-induced killer cells

Abstract

The present invention relates to an expansion method of cytokine-induced killer cells. The expansion method involves providing a peripheral blood mononuclear cell sample; culturing the peripheral blood mononuclear cell sample with a first expansion medium, wherein the first expansion medium comprises interferon-γ, a platelet-rich plasma activation solution (PRP+) and RPMI culture medium; subsequently culturing with a second expansion medium, wherein the second expansion medium comprises interleukin-2, anti-CD3 monoclonal antibody (OKT3), the platelet-rich plasma activation solution (PRP+) and RPMI culture medium; and further culturing with a third expansion medium, wherein the third expansion medium comprises interleukin

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