HPLC workstation, HPLC column of the same and method for flushing out clogged particles of an eluent

Abstract

Systems and methods for flushing out the particles clogged at the inlet of the HPLC column of an HPLC workstation to mitigate / minimize pressure increase and to increase the column lifetime are disclosed. An aspect of the method comprises configuring a flow direction of the HPLC column by configuring a first side as an inlet and a second side as the outlet for flowing the eluent through it. The analysis is run with the configured flow direction at a first flow rate, wherein the eluent particles are clogged at the inlet during the analysis. The flow direction is reversed by switching an injection valve and running the analysis in the reverse direction at a second flow rate. The second flow rate in the HPLC column is increased in a predetermined order and at a predetermined interval by a pump to flush out the clogged particles from the HPLC column.

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