Method of inducing multipotent stem cells from adult dermal fibroblasts by hypothermia

Abstract

The invention relates to a method of inducing multipotent stem cells from human dermal fibroblasts by hypothermia. In an embodiment, after isolating fibroblasts from human dermal tissue and culturing primary cells, hypothemia is applied to a cell and / or a cell sphere in at least one of the stages of an expansion culture step, a floating culture step and a differentiation culture step, and then its differentiation abilities are detected. Nestin, β III tubulin, and tyrosine hydroxylase positive neuron-like cells as well as Alcian blue positive chondrocyte-like cells, can be observed in the cell sphere culture by this method. In addition, the CD9 gene expression of the resultant fibroblast spheres elevate after the hypothemia treatment, for proving that multipotent stem cells can be cultured by this method.

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