Light-sheet fluorescence microscopy system using metasurface and image optimization method thereof

Abstract

The present invention primarily provides a light-sheet fluorescence microscopy (LSFM) system using metasurface, comprising: a light source configured to generate an incident light beam; a metasurface phase modulation element configured to let the incident light beam pass through; and a set of lens arranged corresponding to the metasurface phase modulation element. In addition, the present invention further provides an image optimization method, comprising: a) providing a sample; b) irradiating the sample using the LSFM system; c) acquiring an image of the sample using an image acquisition unit; and d) processing the acquired image using at least one algorithm to generate an optimized image.

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