Composition for promoting hair growth
Camellia kaempferol glycoside composition addresses hair loss by promoting hair follicle cell proliferation and inhibiting apoptosis, providing a safer and effective solution for hair growth.
Patent Information
- Application Number
- TW111138564
- Authority / Receiving Office
- TW · TW
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2021-10-13
- Filing Date
- 2022-10-12
- Publication Date
- 2026-07-11
- Estimated Expiration
- 2042-10-11
AI Technical Summary
Hair loss is increasingly prevalent across all ages and genders due to environmental and physiological factors, with existing treatments like minoxidil and finasteride having side effects, necessitating a need for a biological material that promotes hair growth with fewer side effects.
A composition containing camellia kaempferol glycoside, derived from camellia oil meal, is used to promote hair growth by enhancing the proliferation of hair follicle cells and inhibiting apoptosis of outer root sheath cells.
The composition effectively promotes hair growth and prevents hair loss by stimulating dermal papilla cells and protecting outer root sheath cells, offering a safer alternative to existing treatments.
Smart Images
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Figure IMG-2_DRAW_111138564-A0304-14-0001-2 
Figure IMG-2_DRAW_111138564-A0304-14-0002-3
Abstract
Description
Technical Field
[0001] This specification relates to a composition for promoting hair growth. Prior Technology
[0002] Hair primarily serves to protect the scalp from external irritants such as ultraviolet rays and contributes to a person's aesthetic appearance. However, in modern society, due to environmental pollution, strong ultraviolet radiation, and other natural factors, as well as physiological factors such as stress and hormonal imbalances, hair loss is becoming increasingly common. Unlike in the past when it was limited to adult men, hair loss now affects people of all ages and genders, making materials for the prevention and treatment of hair loss urgently needed.
[0003] Hair follicles, as the physiological organs of hair growth, repeat the hair growth cycle after birth, participating in the growth, maintenance, and shedding of hair. The hair growth cycle is divided into the anagen phase, in which hair is generated and actively grows; the catagen phase, in which hair degenerates; the telogen phase, in which hair is maintained until it falls out; and the exogen phase, in which hair loss occurs.
[0004] The exact cause of hair loss is still unknown, but it has been suggested that it may be due to factors such as the inhibition or weakening of the proliferation and function of dermal papilla cells, which are involved in regulating the hair cycle; abnormal hair cycle caused by the effects of male hormones; abnormal changes in the hair cycle caused by reduced blood flow to the scalp; medications such as anticancer drugs; mental stress; physical stimulation; and environmental pollution.
[0005] As is well known, dermal papilla cells, a type of specialized fibroblast within the hair bulb, interact with various epithelial cells within the hair follicle, playing a crucial role in hair follicle formation, hair regeneration, and hair growth. Therefore, the growth and proliferation of dermal papilla cells, as well as the inhibition of apoptosis, have become important targets in the development of drugs that maintain the hair growth phase and inhibit hair loss.
[0006] Minoxidil and finasteride, previously used as hair growth promoters and hair loss treatments, have been accompanied by side effects such as excessive hair growth and decreased sexual function. Furthermore, the specific mechanism of action of minoxidil remains unclear. Therefore, there is a need to develop biological materials that promote the proliferation of dermal papilla cells while having fewer side effects. Summary of the Invention
[0007] [The problem the invention aims to solve]
[0008] On the one hand, this case aims to provide a composition for promoting hair growth. [Technical means used to solve the problem]
[0009] On the one hand, this case provides a hair growth promoting composition, the active ingredient of which includes camellia kaempferol glycoside.
[0010] In one exemplary implementation, the camellia kaempferol glycoside may be extracted from camellia oil meal.
[0011] In one exemplary implementation, the content of the camellia kaempferol glycoside relative to the total weight of the composition may be from 0.01 to 20 by weight.
[0012] In one exemplary implementation, the camellia kaempferol glycoside can be administered at a dosage of 1 to 10 mg / kg / day.
[0013] In one exemplary implementation, the hair growth promotion may be based on the proliferation of hair follicle cells.
[0014] In one exemplary implementation, the hair follicle cells may be hair papilla cells.
[0015] In one exemplary implementation, the hair growth promotion may be based on inhibiting apoptosis of outer root sheath cells.
[0016] In one exemplary implementation, the composition may be a topical skin composition.
[0017] In one exemplary implementation, the composition may be a food, cosmetic, or pharmaceutical composition. [Effects of the invention]
[0018] On the one hand, the hair growth promoting composition in this case has excellent hair growth promoting effects and prevents hair loss.
[0019] On the one hand, the hair growth promoting composition in this case can promote the proliferation of hair follicle cells and promote hair growth.
[0020] On the one hand, the hair growth promoting composition in this case can inhibit apoptosis of outer root sheath cells and promote hair growth. Simple Explanation of the Diagram
[0021] Figure 1 shows the results of chromatographic analysis of camellia kaempferol glycosides according to one embodiment of this case.
[0022] Figure 2 is a graph showing the efficacy of a hair growth promoting composition according to an embodiment of the present invention in promoting the proliferation of hair papilla cells.
[0023] Figure 3 is a graph illustrating the efficacy of a hair growth promoting composition according to an embodiment of the present invention in inhibiting apoptosis of outer root sheath cells.
[0024] Figure 4 is a graph showing the antioxidant efficacy of a hair growth promoting composition according to an embodiment of the present invention in outer root sheath cells.
[0025] Figure 5 is a graph showing the hair growth results of a hair growth promoting composition according to an embodiment of the present invention.
[0026] Figure 6 is a photograph showing the hair growth results of a hair growth promoting composition according to an embodiment of the present invention. Implementation
[0027] The present invention will now be described in detail.
[0028] On the one hand, this case provides a hair growth promoting composition in which camellia kaempferol glycoside is included as an active ingredient.
[0029] In this case, camellia refers to the camellia tree (Camellia japonica), an evergreen tree belonging to the Theaceae family, mainly distributed in East Asia, including Korea. Camellia oil is extracted from its seeds, and the wood is used as furniture and fine craft materials. In this case, the camellia can be made from all parts of the plant, including roots, stems, leaves, and flowers, or the entire plant (whole herb).
[0030] In this case, camellia kaempferol glycoside, as a kaempferol glycoside derived from camellia, can be prepared by extracting an extract from camellia using a solvent and removing impurities, sugars, and proteins from the extract using an ion exchange resin, but is not limited to this method. This camellia kaempferol glycoside may not contain saponins.
[0031] In one implementation example, the camellia kaempferol glycoside may be extracted from camellia oil meal.
[0032] In one implementation, the solvent may be water, an organic solvent, or a combination thereof. The organic solvent may include, but is not limited to, C1-C6 alcohols, acetone, ethane, ethyl acetate, carbon dioxide, or mixtures thereof.
[0033] In one implementation, the camellia kaempferol glycoside may include one or more selected from the group consisting of camellia kaempferol trisaccharide and camellia kaempferol tetrasaccharide.
[0034] In one implementation example, the camellia kaempferol trisaccharide may include one or more compounds selected from the group consisting of compound 1 of chemical formula 1, compound 2 of chemical formula 2, compound 3 of chemical formula 3, and compound 4 of chemical formula 4. [Chemical Formula 1] [Chemical Formula 2] [Chemical Formula 3] [Chemical Formula 4]
[0035] In one embodiment, compound 1 may be kaempherol-3-O-β-D-xylopyranosyl(1→3)-O-α-L-rhamnopyranosyl(1→6)-O-β-D-galactopyranoside. Compound 2 may be Tsubakioside A. Compound 3 can be kaempherol-3-O-α-L-rhamnopyranosyl(1→6)-O-β-D-xylopyranosyl(1→2)-O-β-D-galactopyranoside. Compound 4 can be camelliaside B.
[0036] In one implementation, the camellia kaempferol tetraglycoside may include one or more compounds selected from the group consisting of compound 5 of chemical formula 5, compound 6 of chemical formula 6, compound 7 of chemical formula 7, and compound 8 of chemical formula 8. [Chemical Formula 5] [Chemical Formula 6] [Chemical Formula 7] [Chemical Formula 8]
[0037] In one implementation, compound 5 may be Tsubakioside C. Compound 6 may be Tsubakioside B. Compound 7 may be Tsubakioside D. Compound 8 may be kaempherol-3-O-β-D-xylopyranosyl(1→3)-O-α-L-rhamnopyranosyl(1→6)-O-β-D-galactopyranosyl-(1→2)-O-β-D-glucopyranoside.
[0038] In one embodiment, the weight ratio of the camellia kaempferol trisaccharide and the camellia kaempferol tetrasaccharide can be 1:1 to 2. For example, the weight ratio of the camellia kaempferol trisaccharide and the camellia kaempferol tetrasaccharide can be 1:1 to 2, 1:1.2 to 2, 1:1.4 to 2, 1:1.4 to 1.8, or 1:1.6 to 1.8.
[0039] In one embodiment, the content of camellia kaempferol glycoside relative to the total weight of the composition can be from 0.01% to 20% by weight. If the content of camellia kaempferol glycoside is less than 0.01% by weight, the hair growth promoting effect may be reduced; if it exceeds 20% by weight, it may increase scalp irritation. Specifically, the content of camellia kaempferol glycoside relative to the total weight of the composition can be 0.01% by weight or more, 0.02% by weight or more, 0.03% by weight or more, 0.04% by weight or more, 0.05% by weight or more, 0.1% by weight or more, 0.2% by weight or more, 0.3% by weight or more, 0.4% by weight or more, 0.5% by weight or more, 0.6% by weight or more, 0.7% by weight or more, 0.8% by weight or more, 0.9% by weight or more, or 1% by weight or more. At the same time, the following concentrations are permitted: 1.5% or more by weight, 2% or more by weight, 2.5% or more by weight, 3% or more by weight, 3.5% or more by weight, 4% or more by weight, 4.5% or more by weight, or 5% or more by weight, and simultaneously: 20% or less by weight, 19.5% or less by weight, 19% or less by weight, 18.5% or less by weight, 18% or less by weight, 17.5% or less by weight, 17% or less by weight, 16.5% or less by weight, 16% or less by weight, 15.5% or less by weight, or 15% or less by weight.
[0040] In one embodiment, the camellia kaempferol glycoside can be administered at a dosage of 1 to 10 mg / kg / day. If the dosage of camellia kaempferol glycoside is less than 1 mg / kg / day, its hair growth promoting effect may be reduced; if it exceeds 10 mg / kg / day, it may increase skin irritation. Specifically, the camellia kaempferol glycoside can be administered at dosages of 1 mg / kg / day or higher, 1.1 mg / kg / day or higher, 1.2 mg / kg / day or higher, 1.3 mg / kg / day or higher, 1.4 mg / kg / day or higher, 1.5 mg / kg / day or higher, 1.6 mg / kg / day or higher, 1.7 mg / kg / day or higher, 1.8 mg / kg / day or higher, 1.9 mg / kg / day or higher, 2 mg / kg / day or higher, and 2.1 mg / kg / day or higher. g / day and above, 2.2mg / kg / day and above, 2.3mg / kg / day and above, 2.4mg / kg / day and above, 2.5mg / kg / day and above, 2.6mg / kg / day and above, 2.7mg / kg / day Above, above 2.8mg / kg / day, above 2.9mg / kg / day, above 3mg / kg / day, above 3.1mg / kg / day, above 3.2mg / kg / day, above 3.3mg / kg / day, 3.4 While administering a dose of 10 mg / kg / day or more, or 9.9 mg / kg / day or less, or 9.8 mg / kg / day or less, or 9.7 mg / kg / day or less, or 9.6 mg / kg / day or less, or 9.5 mg / kg / day or less, or 9.4 mg / kg / day or less, or 9.3 mg / kg / day or less, or 9.2 mg / kg / day or less, or 9.1 mg / kg / day or less, or 9 mg / kg / day or less, or 8.9 mg / kg / day or less, or 8.8 mg / kg / day or less, or 8.7 mg / kg / day or less, or 8.6 mg / kg / day or less, or 8.5 mg / kg / day or less, or 8.4 mg / kg / day or less, or 8.3 mg / kg / day or less, or 8.2 mg / kg / day or less, or 8.1 mg / kg / day or less, or 8 mg / kg / day or less, or 7.5 mg / kg / day or less, or 7 mg / kg / day or less, the dosage may be lower than 3.5 mg / kg / day.
[0041] In one embodiment, the hair growth promotion can be based on the proliferation of hair follicle cells. The hair follicle cells can be dermal papilla cells. Dermal papilla cells interact with various epithelial cells within the hair follicle, playing a crucial role in hair follicle formation, hair regeneration, and hair growth. The composition according to this invention can promote the proliferation of dermal papilla cells, thereby promoting hair growth and preventing hair loss.
[0042] In one embodiment, the hair growth promotion can be based on inhibiting apoptosis of outer root sheath cells. The outer root sheath cells function as cells that protect the hair generated at the hair bulb until it completely ceases keratinization and transport it to the epidermis. The composition according to this invention can inhibit the apoptosis of these outer root sheath cells, thereby promoting hair growth.
[0043] In one implementation, the route of administration of the composition is not limited, and transdermal or topical application is preferred.
[0044] In one embodiment, the composition may be a cosmetic composition. The cosmetic composition may have dosage forms such as, but is not limited to, gentle toners, astringent toners, nourishing toners, nourishing creams, massage creams, eye creams, eye serums, essences, cleansing creams, cleansing milks, cleansing foams, makeup removers, masks, powders, body lotions, moisturizing creams, moisturizing serums, shower gels, hair dyes, shampoos, conditioners, hair styling agents, hair growth products, ointments, gels, creams, patches, sprays, powders, and skin-adhesive types.
[0045] In addition, in each dosage form, besides the essential ingredients, other ingredients can be appropriately selected and formulated by those skilled in the art, depending on the type of other topical agents or the intended use.
[0046] The cosmetic composition can be provided in all dosage forms suitable for topical application. For example, it can be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, a solid, a gel, a powder, a paste, a microneedle, a foam, or an aerosol composition. Such dosage form compositions can be prepared according to general methods in the art.
[0047] In addition to the compounds described herein, the cosmetic compositions according to this specification may further include functional additives and ingredients typically found in cosmetic compositions. These functional additives may include ingredients selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular-weight peptides, high-molecular-weight polysaccharides, sphingolipids, and seaweed concentrates. The cosmetic compositions according to this specification may preferably include other ingredients that can synergistically enhance the main effects, without compromising the primary efficacy. Furthermore, the cosmetic compositions according to this specification may also include moisturizers, emollients, surfactants, UV absorbers, preservatives, bactericides, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cooling agents, or antiperspirants. The formulation amounts of these ingredients can be easily selected by a person skilled in the art without compromising the purpose and effects of this specification, and the formulation amounts, based on the total weight of the composition, may be from 0.001 to 10% by weight, specifically from 0.01 to 3% by weight.
[0048] In one embodiment, the composition may be a food composition. The dosage form of the food composition is not particularly limited; for example, it may be formulated as tablets, granules, pills, powders, liquids such as beverages, caramel, gels, sticks, tea bags, etc. For each dosage form of the food composition, in addition to the active ingredient, those skilled in the art can easily and appropriately select commonly used ingredients in the art to formulate it according to the dosage form or intended use, and when used simultaneously with other raw materials, it can induce a synergistic effect.
[0049] A food composition according to one embodiment may include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents, and thickeners (for cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, and carbonating agents used in carbonated beverages. Additionally, a food composition according to one embodiment may include natural fruit juice and fruit pulp used to make fruit juice beverages and vegetable beverages. The ingredients may be used alone or in combination. While the proportion of the additives is not critical, they typically range from 0 to 50 parts by weight per 100 parts by weight of the composition according to one embodiment.
[0050] In one embodiment, the composition may be a pharmaceutical composition. The pharmaceutical composition can be administered orally, non-orally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, etc. Dosage forms for oral administration may be tablets, pills, soft and hard capsules, granules, powders, fine granules, liquids, emulsions, or balls, but are not limited thereto. Dosage forms for non-oral administration may be solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches, or sprays, but are not limited thereto. The dosage form can be readily prepared according to general methods in the art and may further include surfactants, excipients, wettable powders, emulsification accelerators, suspending agents, salts or buffers for adjusting osmotic pressure, colorants, spices, stabilizers, preservatives, or other commonly used adjuvants.
[0051] On one hand, this application provides a method for promoting hair growth or preventing hair loss, the method comprising administering to an individual who requires promotion of hair growth or prevention of hair loss a composition comprising an effective amount of camellia kaempferol glycoside. On the other hand, the administration of the method may be performed according to the administration method and dosage described in this application.
[0052] On the one hand, this case provides the use of camellia kaempferol glycoside for preparing compositions for promoting hair growth.
[0053] On the one hand, this case provides a hair growth promoting use of camellia kaempferol glycoside.
[0054] The present invention will now be described in more detail through examples and other means. These examples are merely illustrative, and those skilled in the art should understand that the scope of the invention is not limited by these examples. [Example 1] Preparation of Camellia Kaempferol Glycoside
[0055] At low temperature, 1 kg of camellia oil meal (Jeju Island) was pressed to remove oil, and 8 kg of 70% ethanol was added. Extraction was performed at 60°C for 8 hours using a heating extractor, followed by filtration using a filter press to obtain camellia oil meal extract. The extract was concentrated under reduced pressure at 50-60°C using a rotary vacuum concentrator until the solids content was approximately 50%, yielding 200 g of concentrate. The concentrate was diluted with purified water until the solids content was 10%. This solution was loaded onto a glass column packed with 2 L of ion exchange resin (HP-20), and washed with 10 L of purified water to remove impurities, sugars, and proteins. Then, 10 L of 30% ethanol was passed through to obtain the eluent. The eluent was concentrated under reduced pressure and freeze-dried to obtain 20 g of camellia kaempferol glycoside powder. The obtained camellia kaempferol glycoside powder was dissolved in 70% ethanol, and fractionated by reversed-phase chromatography under 0.1% TFA / water / 0.1% TFA / acetonitrile conditions to separate the components contained in each fraction. The separated components are compounds 1 to 4 as camellia kaempferol trisaccharides and compounds 5 to 8 as camellia kaempferol tetrasaccharides. Compounds 1 to 8 were found to each have the chemical formulas 1 to 8. The results of the reversed-phase chromatography are shown in Table 1 and Figure 1. [Chemical Formula 1] [Chemical Formula 2] [Chemical Formula 3] [Chemical Formula 4] [Chemical Formula 5] [Chemical Formula 6] [Chemical Formula 7] [Chemical Formula 8] [Table 1] Retention time (minute) area (%) Compound 5 + Compound 7 33.800 28.99 Compound 6 35.760 11.13 Compound 8 37.067 23.28 Compound 1 + Compound 2 45.585 18.92 Compound 3 + Compound 4 46.846 17.67 [Experimental Example 1] Evaluation of the effect of promoting the proliferation of hair follicle dermal papilla cells
[0056] Hair follicle papilla cells were isolated from human scalp tissue at the back of the head using a microscope and cultured for 14 days in culture dishes coated with collagen type I. After 72 hours of culture in DMEM (Dulbecco's modified eagles medium; HyClone GE Healthcare) containing antibiotics (1%), fungizone (0.1%), and 20% FBS, at 37°C and 5% CO2, the medium was replaced with DMEM containing 10% FBS. When the cells reached more than 90% confluence in the culture dish, the cultured cells were collected using 0.25% trypsin / 10mM EDTA (Welgene) and cultured for another 72 hours in DMEM containing 10% FBS, at 37°C and 5% CO2, before being used in subsequent experiments.
[0057] After seeding the cultured hair follicle papilla cells at 2000 cells / well in 96-well plates, they were cultured for 24 hours at 5% CO2 and 37°C. Camellia kaempferol glycosides from Example 1 were then treated with various concentrations (1 ppm, 2 ppm, 5 ppm, 10 ppm, 20 ppm, and 50 ppm) and cultured for 72 hours at 5% CO2 and 37°C. An untreated group served as the control. The cells were then cultured in CCK-8 (DONGJIN) for 2 hours, and the absorbance was measured at 450 nm using a microplate reader. The measured absorbance values were used to set the control value to 100, and the relative cell viability was calculated, as shown in Figure 2. [Experimental Example 2] Evaluation of the efficacy of inhibiting apoptosis in outer root sheath cells
[0058] Outer root sheath cells were isolated from human scalp tissue at the back of the head using a microscope and cultured for 14 days in culture dishes coated with collagen type I. After 72 hours of culture in DMEM (Dulbecco's modified eagles medium; HyClone GE Healthcare) containing antibiotics (1%), fungizone (0.1%), and 20% FBS, at 37°C and 5% CO2, the culture was replaced with epilife medium containing EDGS. When the cells reached over 90% confluence in the culture dish, they were collected using 0.25% trypsin / 10mM EDTA (Welgene), transferred to epilife medium containing EDGS, and cultured for another 72 hours at 37°C and 5% CO2 (until the cells reached over 90% confluence) before being used in subsequent experiments.
[0059] After treating the camellia kaempferol glycosides from Example 1 at various concentrations (1 ppm, 2 ppm, 5 ppm) for 4 hours, they were further incubated with DKK1, H2O2, and TGF beta 2, known hair loss inducing factors, and cultured overnight. Then, after culturing in CCK-8 (DONGJIN) for 2 hours, the absorbance was measured at 450 nm using a microplate reader. The absorbance was used to set the control group (value = 100), and relative cell viability was calculated, as shown in Figure 3. The control group represents the group that received no treatment. [Experimental Example 3] Evaluation of antioxidant efficacy in outer root sheath cells
[0060] In Experiment 2, for the experiment, cultured outer root sheath cells were seeded at 10,000 cells / well onto 8-well slides and cultured at 37°C and 5% CO2 for 24 hours. After treatment with kaempferol glycosides (5 ppm) from Example 1, further culture was terminated, the cell culture medium was removed, and formalin solution was added for fixation at room temperature for at least 15 minutes. The fixative was removed, and the cells were washed three times within 3 minutes using washing buffer (PBS containing 0.1% BSA). After incubation at room temperature for 30 minutes using a permeabilization solution, the cells were reacted at room temperature for 30-60 minutes using blocking buffer (PBS containing 0.1-1% BSA). The antibody against the target protein (NRF2) was diluted in blocking buffer and incubated overnight at 4°C. After being removed and placed at room temperature, the antibody solution was removed once and washed four times within 3 minutes using washing buffer (PBS containing 0.1% BSA). The secondary antibody (conjugated with fluorescent dye) capable of targeting the primary antibody was diluted in blocking buffer and reacted at room temperature for 2 hours. Then, a mounting solution was applied, a coverslip was placed to prevent air bubbles, and the results were observed using a fluorescence microscope. Figure 4 shows the results. A control group (no treatment) was used.
[0061] As can be confirmed by Figure 4, when kaempferol glycosides are treated, the NRF2 protein (J Invest Dermatol. 2017 Feb;137(2):295-304, Oxidative Damage Control in a Human (Mini-) Organ: Nrf2 Activation Protects against Oxidative Stress-Induced Hair Growth Inhibition), a well-known antioxidant marker, moves into the nucleus of outer root sheath cells (arrows in Figure 4 indicate the movement of NRF2 protein into the nucleus). This confirms that kaempferol glycosides can inhibit apoptosis of outer root sheath cells. [Experimental Example 4] Evaluation of the efficacy of hair follicle length growth
[0062] Twenty hair follicle samples were isolated from each experimental group from 57-year-old women. Six isolated hair follicle samples were cultured in each well of 24-well plates on 500 μL of William's E medium containing 2 mM L-glutamine, 10 ng / mL hydrocortisone, 1% antibiotics, and 0.1% fungizone B. The 24-well plates were treated with camellia saponin B1 (0.1 ppm), camellia saponin B2 (0.1 ppm), and those described in Example 2 (0.01 ppm, 0.1 ppm). Untreated samples served as a control. The medium was replaced every 2–3 days. Hair growth length was measured on day 2 and day 5, as shown in Figure 5. Hair follicles were photographed using a stereomicroscope (Dongwon CNS, Korea) on day 7, as shown in Figure 6. [Dosage Form Example 1] Soap
[0063] Soap preparations are made using general methods according to the components listed in Table 2 below. [Table 2] Element content Camellia kaempferol glycosides in Example 1 1.5g Titanium dioxide 0.5g polyethylene glycol 1g glycerin 0.5g EDTA-1,5-diaminetetraacetic acid 0.5g sodium 1g pigment 0.2g Soap scent 0.2g soap base 94.1g [Dosage Form Example 2] Liquid Ampoules
[0064] Liquid ampoules are prepared using conventional methods according to the components listed in Table 3 below. [Table 3] Element content Camellia kaempferol glycosides in Example 1 50mg Difficult-to-digest maltodextrin 100mg Citric acid 10mg spices 5mg purified water 28.5g [Dosage Form Example 3] Cream
[0065] Prepare the cream using the general method according to the components in Table 4 below. [Table 4] Element content Camellia kaempferol glycosides in Example 1 3g Polyethylene glycol monostearate 4g Self-emulsifying glyceryl monostearate 10g cetyl alcohol 8g squalane 10g Tri-2-ethylhexaneglyceride 10g Neurosphingolipids 2g 1,3-Butanediol 14g purified water 189g [Dosage Form Example 4] Cosmetic Liquid Formulation
[0066] Prepare a cosmetic serum formulation using a standard method according to the components listed in Table 5 below. [Table 5] Element content Camellia kaempferol glycosides in Example 1 3g Hydroxyethyl cellulose 15g Xanthan Gum 4g 1,3-Butanediol 10g glycerin 8g Sodium hyaluronate 5g purified water 155g [Dosage Form Example 5] Soft Capsules
[0067] A soft capsule was prepared by mixing 60 mg of camellia kaempferol glycoside from Example 1, 160 mg of L-carnitine, 320 mg of soybean oil, 2 mg of palm oil, 8 mg of hydrogenated vegetable oil, 4 mg of beeswax, and 6 mg of lecithin and filling the mixture into one capsule using conventional methods. [Dosage Form Example 6] Tablets
[0068] Mix 60 mg of camellia kaempferol glycoside from Example 1, 500 mg of galactooligosaccharide, 80 mg of lactose, and 220 mg of maltose, granulate using a fluidized bed granulator, then add 6 mg of sugar ester, and compress the mixture into tablets using a tableting machine. [Dosage Form Example 7] Granules
[0069] 60 mg of camellia kaempferol glycoside from Example 1, 250 mg of anhydrous crystalline glucose, and 550 mg of corn starch were mixed, and granules were prepared using a fluidized bed granulator. The granules were then filled into bags to prepare granules. [Dosage Form Example 8] Beverage
[0070] After mixing 60 mg of camellia kaempferol glycoside from Example 1, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 500 ml of purified water was added, and 200 ml was filled into each bottle. After filling the bottles, the mixture was sterilized at 130°C for 4-5 seconds to prepare the beverage.
[0071] This case further illustrates this point through the following implementation example, which does not limit the scope of the request items.
[0072] Example 1. Use of camellia kaempferol glycoside for preparing hair growth promoting compositions.
[0073] Example 2. An application, which is the same as the application of Example 1, wherein the camellia kaempferol glycoside is extracted from camellia oil meal.
[0074] Example 3. An application, which is an application of either Example 1 or 2, wherein the camellia kaempferol glycoside comprises one or more selected from the group consisting of camellia kaempferol trisaccharide and camellia kaempferol tetrasaccharide.
[0075] Example 4. An application in which, as in any of Examples 1 to 3, the content of the camellia kaempferol glycoside is 0.01 to 20 by weight relative to the total weight of the composition.
[0076] Example 5. One use, as in any of Examples 1 to 4, is that the dosage of the camellia kaempferol glycoside is 1 to 10 mg / kg / day.
[0077] Example 6. An application, which is one of the applications of Examples 1 to 5, wherein the hair growth promotion is based on the proliferation of hair follicle cells.
[0078] Example 7. An application, which is the same as the application of Example 6, wherein the hair follicle cells are hair papilla cells.
[0079] Example 8. An application, which is one of the applications of Examples 1 to 7, wherein the hair growth promotion is based on inhibiting apoptosis of outer root sheath cells.
[0080] Example 9. An application, which is one of the applications of Examples 1 to 8, wherein the composition is a topical skin composition.
[0081] Example 10. An application, which is one of the applications of Examples 1 to 8, wherein the composition is a food composition.
[0082] Example 11. An application, which is one of the applications of Examples 1 to 9, wherein the composition is a cosmetic composition.
[0083] Example 12. An application, which is one of the applications of Examples 1 to 9, wherein the composition is a pharmaceutical composition.
[0084] none
[0085] Domestic storage information (please note in order of storage institution, date, and number) none Overseas storage information (please note in the order of storage country, institution, date, and number) none
Claims
1. Use of camellia kaempferol glycoside for preparing hair growth promoting compositions.
2. According to the purpose of claim 1, the camellia kaempferol glycosides are extracted from camellia oil meal.
3. According to the purpose of claim 1, the camellia kaempferol glycoside includes one or more selected from the group consisting of camellia kaempferol trisaccharide and camellia kaempferol tetrasaccharide.
4. According to the use of claim 1, the content of said camellia kaempferol glycoside is 0.01 to 20% by weight relative to the total weight of the composition.
5. According to the use of claim 1, the dosage of said camellia kaempferol glycoside is 1 to 10 mg / kg / day.
6. As claimed in claim 1, the hair growth promotion is based on the proliferation of hair follicle cells.
7. As claimed in claim 6, the hair follicle cells are dermal papilla cells.
8. As claimed in claim 1, the hair growth promotion is based on inhibiting apoptosis of outer root sheath cells.
9. The use according to any one of claims 1 to 8, wherein the composition is a topical skin composition.
10. The use according to any one of claims 1 to 8, wherein the composition may be a food, cosmetic, or pharmaceutical composition.