Screening methods for components that promote LTBP-1 expression and substances that promote LTBP-1 expression.

Abstract

This invention aims to provide a composition for promoting LTBP-1 expression and a method for screening substances that promote LTBP-1 expression. Specifically, this invention relates to a composition for promoting LTBP-1 expression, which comprises extracts of one or more plants selected from the group consisting of rose, gentian, houttuynia cordata, calamus, perilla, sage, and star fruit, wherein the rose is a rose containing roseocyanin and / or delphinidin glycoside.

Description

[Technical Field] This invention relates to a composition for promoting the expression of LTBP (Latent TGFβ binding protein)-1, a method for screening substances that promote the expression of LTBP-1, and a method for promoting the expression of LTBP-1. [Previous Technology] Tissues such as skin, blood vessels, lungs, and ligaments require the property of recovering their elasticity after being stretched. This elasticity is maintained by the structure of cells and the extracellular matrix. The extracellular matrix includes collagen fibers, glycosaminoglycans (hyaluronic acid, keratin sulfate, heparin sulfate, chondroitin, chondroitin sulfate, dermatan sulfate, etc.), and elastic fibers. While collagen fibers and glycosaminoglycans are strong, they cannot stretch or contract under external forces. On the other hand, elastic fibers are elastic. Therefore, in tissues such as skin that require stretching or contraction, elastic fibers are a crucial factor in imparting elasticity. Generally speaking, in the case of elastic fibers, elastin is deposited around the fibers called microfibrils, which can cross-link. The turnover of elastic fibers is extremely slow. If elastic fibers decompose or degenerate due to aging or exposure to ultraviolet rays, arteriosclerosis, emphysema, and loose skin may occur. In recent years, the intermolecular interaction between fibrin proteins that promote elastic fiber formation and LTBP (latent TGF-β-binding protein) has attracted much attention. LTBP belongs to the same superfamily of proteins as fibrin. It is known that there are four family members of LTBP: LTBP-1 to LTBP-4, characterized by a TGF-β binding 8-Cys domain. LTBP-2 does not bind to latent TGF-β; while LTBP-1 and LTBP-3 bind to inert latent TGF-β, thus storing TGF-β in an inert state, which can be mobilized when needed. Regarding LTBP-4, it has been reported that it interacts with fibrin-5 and is related to the formation of elastic fibers (Patent Document 1 and Non-Patent Document 1). [Prior Art Documents] [Patent Documents] [Patent Document 1] Japanese Patent No. 6050573 [Non-Patent Document] [Non-Patent Literature 1] Noda k et al., Proc Natl Acad Sci USA., 110, 2852-7, 2013 [Summary of the Invention] [The problem that the invention aims to solve] Although some researchers have pointed out the association between the LTBP family and elastic fiber formation, the role of LTBP-1 in elastic fiber formation remains largely unknown. Therefore, the inventors of this invention focused on LTBP-1 to investigate its effect on elastic fiber formation. As a result, as shown in the examples described later, LTBP-1, unlike LTBP-4, independently still has the effect of promoting elastic fiber formation. Therefore, it is concluded that substances that can promote the performance of LTBP-1 are capable of promoting elastic fiber formation. However, no substances that can promote the performance of LTBP-1 have yet been reported. This invention aims to provide a composition for promoting LTBP-1 expression. Furthermore, this invention aims to provide a method for screening substances that promote LTBP-1 expression. [Means for Solving the Problem] The inventors of this invention, through dedicated research to solve the aforementioned problems, discovered that extracts from specific plants such as Gentiana scabra, Houttuynia cordata, and Rosa rugosa containing anthocyanin and / or delphinidin compounds have the effect of promoting LTBP-1 expression, thus completing this invention. That is, the present invention includes the following components for promoting LTBP-1 expression. [1] A component for promoting LTBP-1 expression, comprising extracts of one or more plants selected from the group consisting of rose, gentian, houttuynia cordata, calamus, perilla, sage and star fruit, wherein the rose is a rose containing rose anthocyanin and / or delphinidin glycoside. [2] The component for promoting LTBP-1 expression as described in [1] above, wherein the extracts of the plants are extracts of one or more plants selected from the group consisting of gentian, houttuynia cordata, and rose containing rose anthocyanin and / or delphinidin glycoside. [3] The component for promoting LTBP-1 expression as described in [1] or [2] above, is for promoting LTBP-1 expression in the skin. [4] Any of the ingredients in [1] to [3] above for promoting LTBP-1 expression is in the skin and is used to promote the expression of LTBP-1 that has decreased due to exposure to ultraviolet radiation. [5] Any of the ingredients in [1] to [4] above for promoting LTBP-1 expression is used to prevent or improve the reduction of elastic fibers caused by exposure to ultraviolet radiation by promoting the expression of LTBP-1 in the skin. [6] Any of the ingredients in [1] to [5] above for promoting LTBP-1 expression is a topical skin agent. [7] Any of the ingredients in [1] to [6] above for promoting LTBP-1 expression is a cosmetic. [8] Any of the ingredients in [1] to [5] above for promoting LTBP-1 expression is a food or beverage. [9] A method for screening a substance that promotes LTBP-1 expression, comprising: irradiating skin cells with ultraviolet light in vitro; culturing the irradiated skin cells in a test culture medium containing the test substance or a control culture medium without the test substance; and comparing the expression levels of LTBP-1 in the test skin cells cultured in the test culture medium and the control skin cells cultured in the control culture medium; when the expression level of LTBP-1 in the test skin cells is greater than the expression level of LTBP-1 in the control skin cells, selecting the test substance as a substance that promotes LTBP-1 expression.

[10] A method for promoting LTBP-1 expression, comprising applying an extract of one or more plants selected from the group consisting of rose, gentian, houttuynia cordata, calamus, perilla, sage, and star fruit to a target, wherein the rose is a rose containing rose anthocyanin compound and / or rose delphinidin compound.

[11] An extract of one or more plants selected from the group consisting of rose, gentian, houttuynia cordata, calamus, perilla, sage and star fruit for the purpose of promoting the expression of LTBP-1, wherein the rose is a rose containing rose anthocyanin compound and / or rose delphinidin compound. [Effects of the invention]. According to the present invention, a composition for promoting LTBP-1 expression can be provided. According to the present invention, LTBP-1 expression can be promoted. Furthermore, according to the present invention, a method for screening substances having the effect of promoting LTBP-1 expression can be provided, etc.

Implementation Method

Claims

1. Use of a plant extract for preparing a composition that promotes the expression of LTBP-1 in the skin, wherein the extract comprises an extract of rose and one or more plant extracts selected from the group consisting of houttuynia cordata and sage, wherein the rose is a rose containing a rosacyanin compound and / or a rosadelphine compound.

2. As claimed in claim 1, wherein the aforementioned composition is used to promote the formation of elastic fibers by promoting the expression of LTBP-1 in the skin, which is reduced due to exposure to ultraviolet radiation.

3. As claimed in claim 1, wherein the aforementioned composition is used to prevent or improve the reduction of elastic fibers caused by exposure to ultraviolet radiation by promoting the expression of LTBP-1 in the skin.

4. The use of any of claims 1 to 3, wherein the aforementioned composition is a topical skin agent.

5. The use of any of the claims 1 to 3, wherein the aforementioned components are cosmetics.

6. The use of any of the claims 1 to 3, wherein the aforementioned components are food or beverages.

7. A non-therapeutic method for promoting the expression of LTBP-1 in the skin, comprising applying a rose extract and one or more plant extracts selected from the group consisting of Houttuynia cordata and Sage to a subject, wherein the rose is a rose containing rose anthocyanin compound and / or delphinidin compound.

8. A non-therapeutic use of a plant extract for promoting the expression of LTBP-1 in the skin, wherein the extract comprises an extract of rose and one or more plant extracts selected from the group consisting of houttuynia cordata and sage, wherein the rose is a rose containing rose anthocyanin compound and / or delphinidin compound.

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