Miniature modular detachable image observation base device

Abstract

A miniaturized, modular, detachable imaging observation base device is designed by placing a high-precision compound lens unit within a wearable optical base. The base has a curved surface that matches the natural curvature of the mouse skull, allowing the compound lens unit to be securely fixed to the mouse's head. By replacing different compound lens units, it can adapt to observation applications at different depths. This overcomes the limitations of superficial brain regions, allowing the miniaturized, graded-index lens to penetrate deep into the mouse brain with its slender shape, reaching deep brain regions. This enables functional imaging monitoring of deep brain region neural cell activity over multiple and extended periods.

Claims

1. A miniature modular detachable image observation base device for nonlinear optical high-resolution neural image monitoring, comprising: A compound lens unit is composed of a miniaturized graded refractive index lens and a miniaturized coupling lens; The device also includes a wearable optical base with a curved surface at the lower edge that matches the natural curvature of the mouse skull. Several magnetic holes are provided on the upper part of the base, which are used to embed a corresponding number of magnets to achieve a stable fixation through magnetic attraction. The composite lens unit is detachably installed in the wearable optical base, allowing the wearable optical base to stably fix the composite lens unit to the mouse head. By replacing different composite lens units, it can adapt to observation applications at different depths, thus overcoming the limitations of superficial brain regions. The miniaturized graded refractive index lens, with its slender shape, can penetrate deep into the mouse brain to reach deep brain regions, enabling functional imaging monitoring of deep brain region nerve cell activity over multiple and long periods of time.

2. The miniature modular detachable image observation base device described in claim 1, weighing less than 1 gram.

3. The miniature modular detachable image observation base device as described in claim 1, wherein, The wearable optical base has a screw mounting sleeve on the side, which can be fitted with screws to fix and calibrate the base.

4. The miniature modular detachable image observation base device as described in claim 1, wherein, The upper part of the wearable optical base is further provided with several anchor points and filling holes for fixing materials. Screws are placed as anchor points to fix the mouse head shell, and filling materials are placed to enhance fixation and reduce micro-movements.

5. The miniature modular detachable image observation base device as described in claim 4, wherein, The filling material is a dental material and a biocompatible colloid.

6. The miniature modular detachable image observation base device as described in claim 1, wherein, The wearable optical base has a lens placement slot inside to accommodate the miniaturized coupling lens. The lens placement slot is connected to an extension lens support tube. The miniaturized graded refractive index lens passes through the extension lens support tube and penetrates deep into the brain region of the mouse.

7. The miniature modular detachable image observation base device as described in claim 6, wherein, The length of the extended lens support tube is less than 2 mm.

8. The miniature modular detachable image observation base device described in claim 1, further equipped with a pulsed laser scanning module, constitutes a miniaturized nonlinear microscope, realizing functional image monitoring of deep brain regions with micron-level resolution.

9. The miniature modular detachable image observation base device as described in claim 8, wherein, This miniaturized nonlinear microscope is a miniaturized two-photon microscope.

10. The miniature modular detachable image observation base device as described in claim 1, wherein, The wearable optical base has several slots on its side, which are convenient for people to hold against when removing the wearable optical base, so as to increase the force points and thus assist in removing the wearable optical base.

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