Method for manufacturing microfluidic detection substrate, microfluidic detection substrate, and detection device

Abstract

This invention provides a method for manufacturing a microchannel detection patch, the microchannel detection patch, and a detection device. The method includes processing a first light guide sheet to form a first blind hole and a first microchannel; processing a second light guide sheet to form a first through hole corresponding to the first blind hole; placing a first waterproof ring between the first through hole and the first blind hole; and stacking a second light guide sheet on the first light guide sheet and bonding them to form a microchannel detection patch in which the first waterproof ring is embedded. The detection device includes a microchannel detection patch and a fluid introduction element, comprising a plate and a first injection structure disposed thereon. The first injection structure has a first liquid injection element protruding from one side surface of the plate, for insertion into the first through hole to inject liquid into the first microchannel. This achieves rapid and stable connection of the injection port, effectively reducing the risk of liquid leakage.

Claims

1. A method for manufacturing a microchannel detection sheet, comprising the following steps: a first processing step: forming at least one first blind hole and at least one first microchannel on a first light guide sheet, wherein the first blind hole and the first microchannel are interconnected; a second processing step: forming at least one first through hole on a second light guide sheet, wherein the positions of the first through hole and the first blind hole correspond to each other; a placement step: placing a first waterproof ring between the first through hole and the first blind hole; and a bonding step: stacking the second light guide sheet on the first light guide sheet and bonding them to form a microchannel detection sheet in which the first waterproof ring is embedded.

2. A method for manufacturing the microfluidic detection sheet as described in claim 1, wherein, The first processing step further includes forming at least one second blind hole in the first light guide sheet; the second processing step further includes forming at least one second through hole and at least one second microchannel in the second light guide sheet, wherein the second through hole and the second microchannel are interconnected and the positions of the second through hole and the second blind hole correspond to each other; the placement step further includes placing a second waterproof ring between the second through hole and the second blind hole; the microchannel detection sheet formed in the bonding step is further embedded with the second waterproof ring.

3. A method for manufacturing the microfluidic detection sheet as described in claim 1, wherein, The first blind hole is slightly larger than the first through hole, and during the placement step, the first waterproof ring is placed inside the first blind hole.

4. A microchannel detection sheet, comprising: a first light guide sheet having at least one first blind hole and at least one first microchannel, wherein the first blind hole and the first microchannel are interconnected; a second light guide sheet stacked on the first light guide sheet and having at least one first through hole, wherein the positions of the first through hole and the first blind hole correspond to each other, wherein the first light guide sheet and the second light guide sheet are integrally formed by bonding; and at least one first waterproof ring embedded between the first through hole and the first blind hole.

5. The microchannel detection sheet as described in claim 4 further includes at least one second waterproof ring, and the first light guide sheet further has at least one second blind hole, the second light guide sheet further has at least one second through hole and at least one second microchannel, the second through hole and the second microchannel are interconnected, and the positions of the second through hole and the second blind hole correspond to each other; the second waterproof ring is embedded between the second through hole and the second blind hole.

6. The microchannel detection sheet as described in claim 4, wherein, The first blind hole is slightly larger than the first through hole.

7. A detection device, comprising: a microchannel detection sheet including a first light guide sheet, a second light guide sheet, and at least a first waterproof ring, the first light guide sheet having at least a first blind hole and at least a first microchannel, and the first blind hole and the first microchannel being interconnected; a second light guide sheet stacked on the first light guide sheet and having at least a first through hole, the positions of the first through hole and the first blind hole corresponding to each other, wherein the first light guide sheet and the second light guide sheet are integrally formed by bonding; the first waterproof ring being embedded between the first through hole and the first blind hole; and a fluid introduction element including a plate and at least a first injection structure, the first injection structure being disposed on the plate and having a first liquid injection element protruding from one side surface of the plate for insertion into the first through hole to inject liquid into the first microchannel; wherein... The first injection component is a hollow tube with an inner diameter of 2±0.2mm.

8. The detection apparatus as described in claim 7, wherein, The microchannel detection sheet further includes at least one second waterproof ring, and the first light guide sheet further has at least one second blind hole. The second light guide sheet further has at least one second through hole and at least one second microchannel. The second through hole and the second microchannel are interconnected, and the positions of the second through hole and the second blind hole correspond to each other. The second waterproof ring is embedded between the second through hole and the second blind hole. The fluid introduction element further includes at least one second injection structure. The second injection structure is disposed on the plate and has a second liquid injection element protruding from one side surface of the plate for insertion into the second through hole to inject liquid into the second microchannel.

9. The detection apparatus as described in claim 7, wherein, The first blind hole is slightly larger than the first through hole.

Images