Composition and preparation method for inhibiting protein carbamylation, and application thereof

Abstract

This invention provides a composition for inhibiting protein aminomethanization. The composition comprises a first component and a second component. The first component contains extracts of *Caragana korshinskii* (Apiaceae), *Brassica oleracea* (Brassica oleracea), *Vaccinium bracteatum* (Ericaceae), and *Spinacia* (Amaranthaceae) as active ingredients. The second component contains an extract of *Siegesbeckia orientalis* (Asteraceae) as an active ingredient. The mass ratio of the active ingredients in the first component to the active ingredients in the second component is 1:1000 to 80:1. Through the synergistic inhibitory effect of the two components, the composition of this invention can significantly delay the rate of protein aminomethanization. This application also provides a method for preparing the above composition and its application in the preparation of products that inhibit protein aminomethanization.

Claims

1. A composition for inhibiting protein aminomethanization, comprising: The first component and the second component; wherein the first component contains carrot extract, cauliflower extract, blueberry extract and spinach extract as active ingredients; and the second component contains Siegesbeckia orientalis extract as an active ingredient.

2. The composition according to claim 1, wherein, The first component comprises, by volume, ≥0.00025 w / v% and ≤5 w / v% of carrot extract, ≥0.00025 w / v% and ≤5 w / v% of cauliflower extract, ≥0.00025 w / v% and ≤5 w / v% of blueberry extract, and ≥0.00025 w / v% and ≤5 w / v% of spinach extract, with the balance being physiologically acceptable media; the second component comprises, by volume, ≥0.00025 w / v% and ≤100 w / v% of Siegesbeckia orientalis extract, with the balance being physiologically acceptable media; or the second component is Siegesbeckia orientalis extract; wherein the mass ratio of the active ingredient of the first component to the active ingredient of the second component is 1:1000 to 80:

1.

3. The composition according to claim 2, wherein, The first component comprises, by volume, 0.0005 w / v% and 5 w / v% of carrot extract, 0.0005 w / v% and 5 w / v% of cauliflower extract, 0.0005 w / v% and 5 w / v% of blueberry extract, and 0.0005 w / v% and 5 w / v% of spinach extract; the second component comprises, by volume, 0.0005 w / v% and 80 w / v% of Siegesbeckia orientalis extract; the mass ratio of the active ingredient in the first component to the active ingredient in the second component is 1:100 to 30:

1.

4. A method for preparing a composition according to any one of claims 1 to 3, characterized in that it comprises the following steps: diluting each active ingredient of the first component with a physiologically acceptable medium to a first solution of greater than or equal to 0.00025 w / v% and less than or equal to 2 w / v%, and adding the second component to the first solution to achieve a final concentration of greater than or equal to 0.00025 w / v% and less than or equal to 4 w / v%, thereby obtaining the composition.

5. Use of a composition as described in any one of claims 1 to 3 in the preparation of a product that inhibits protein aminomethanization.

6. The application according to claim 5, wherein, The product in question is a drug, medical device, or cosmetic.

7. The application according to claim 5 or 6, wherein, The product in question is an anti-aging and / or skin whitening product.

8. An in vitro method for inhibiting aminomethionization of proteins in mammalian somatic cells, comprising the step of contacting the composition according to any one of claims 1 to 3 with the somatic cells of the mammal.

9. The ex vivo method according to claim 8, wherein, The contact occurs before or after the aminomethionization of proteins in the somatic cells.

10. The ex vivo method according to claim 8, wherein, The somatic cells are skin fibroblasts.

11. The ex vivo method according to claim 8, wherein, During the contact, the composition is used in an amount ranging from 0.000138 mg / 10,000 cells to 2.1 mg / 10,000 cells.

12. A product for preventing, inhibiting, preventing or antagonizing protein aminomethionization in mammalian somatic cells, comprising a composition according to any one of claims 1 to 3 and a pharmaceutically or cosmetically acceptable excipient.

13. The product according to claim 12, wherein, The product in question is a cosmetic, a pharmaceutical, or a medical device.

14. The product according to claim 13, wherein, The product in question is an anti-aging and / or skin whitening product.

Images