Uses of cashew testa extract for preparing scalp care composition

Abstract

One use of an extract of Anacardium occidentale fruit membrane for the preparation of hair care compositions. The extract is obtained by solvent extraction of the membrane of Anacardium occidentale fruit.

Description

Use of the pericarp extract of Anacardium occidentale in the preparation of a hair care composition The present invention relates to the application of Anacardium occidentale, and particularly to a use of the pericarp extract of Anacardium occidentale in the preparation of a hair care composition. Since the rise of the concept of organic and / or natural diets, biotechnology companies and food manufacturers have actively invested in the research and development of related products of natural plants. In order to have a scientific verification basis for the beneficial effects of plant-related products on physical health, the analysis of active ingredients and the evaluation of efficacy of plants have become key projects in product development. Anacardium occidentale ), also known as cashew nut, is a popular nut. The Anacardium occidentale tree is native to northern Brazil and is now cultivated in many tropical and subtropical regions, such as Vietnam. Anacardium occidentale has rich nutritional value. The cashew nut kernel contains rich unsaturated fatty acids, especially monounsaturated fatty acids such as oleic acid. In addition, Anacardium occidentale also contains nutrients such as vitamin E, vitamin K, vitamin B6, calcium, magnesium, potassium, and antioxidants. In the field of hair health care, people increasingly attach importance to obtaining necessary nutrients from diet to promote the health of the scalp and hair. Although there are already many care products on the market and people are increasingly aware of the impact of nutrients in diet on hair, there is still room for improvement in the relevant products on the market to meet the needs of consumers. Therefore, hair-related products have received the attention and favor of the vast number of consumers. Therefore, the inventor actively researches and develops the application of Anacardium occidentale in hair-related products. In some embodiments, there is provided a use of the pericarp extract of Anacardium occidentale in the preparation of a hair care composition. The pericarp extract of Anacardium occidentale is prepared by extracting the pericarp of Anacardium occidentale with a solvent. In some embodiments, in the extraction step, the solid-liquid ratio of the pericarp of Anacardium occidentale to water is 5 to 20:1 to 5, the extraction temperature is 80°C to 90°C, and the extraction time is 80 minutes to 120 minutes. In some embodiments, the pericarp extract of Anacardium occidentale has the ability to increase hair follicle health. In some embodiments, the pericarp extract of Anacardium occidentale increases hair follicle health by enhancing the antioxidant capacity of hair follicle cells. In some embodiments, the pericarp extract of Anacardium occidentale increases hair follicle health by promoting the proliferation of hair follicle cells. In some embodiments, the aforementioned pericarp extract of Anacardium occidentale is at least one compound selected from epicatechin coumarate, phloroglucinol, protocatechuic acid, and naringenin 7-O-(6”-O-p-coumaroyl)-β-D-glucoside. In some embodiments, the pericarp extract of Anacardium occidentale increases hair follicle health by increasing hair follicle angiogenesis. In some embodiments, the Jie Shou fruit membrane extract increases hair follicle health by promoting hair growth gene expression. In some embodiments, the Jie Shou fruit membrane extract increases hair follicle health by reducing hair loss gene expression. In some embodiments, the Jie Shou fruit membrane extract increases the health of the hair follicle by reducing the blood MDA concentration. In some embodiments, the Jie Shou fruit membrane extract has the ability to increase hair diameter. In summary, according to any of the embodiments, the Jie Shou fruit membrane extract can be used to prepare a hair care composition. In other words, when the obtained hair care composition is applied to an individual, the hair care composition can improve or enhance the hair health condition on this individual, that is, the aforementioned hair care composition has the function of hair care. In some embodiments, the Jie Shou fruit membrane extract also has one or more of the following abilities: improving the antioxidant capacity of hair follicle cells, increasing hair follicle angiogenesis, promoting hair growth gene expression, reducing hair loss gene expression, and increasing hair diameter. Some specific embodiments of the present case will be described below. Without departing from the spirit of the present case, the present case can be practiced in many different forms, and the protection scope should not be limited to the conditions specifically stated in the specification. In some embodiments, the Jie Shou fruit membrane extract is obtained by performing an extraction process on the membrane of the Jie Shou fruit. In some embodiments, the membrane of the Jie Shou fruit for extraction is the Jie Shou fruit kernel membrane (also known as the Jie Shou fruit seed coat, Jie Shou fruit peel). The Jie Shou fruit kernel membrane is one or a combination of the thin film, membranous material, outer skin, or seed shell inside the Jie Shou fruit, and hereinafter is collectively referred to as the Jie Shou fruit membrane. In some embodiments, in the extraction process, the Jie Shou fruit membrane for extraction can be the raw material (e.g., a complete Jie Shou fruit membrane), or in a form of being physically pre-treated and decomposed into small sizes such as fragments, granules, or powders. The physical pre-treatment adopted can include at least one of the following: coarse crushing, chopping, shredding, pounding, and grinding. In some embodiments, the solvent can be water, alcohols, or a combination thereof. In the extraction process, the Jie Shou fruit membrane is extracted with the solvent at 80°C to 90°C for 80 minutes to 120 minutes to obtain a primary extract. For example, the Jie Shou fruit membrane can be soaked in water at 85 ± 5°C for 90 minutes to dissolve the effective components of the Jie Shou fruit membrane into the solvent to obtain a primary extract. In some embodiments, in the extraction process, the weight ratio of the Jie Shou fruit membrane to the solvent is 5 to 20:1 to 5. For example, the weight ratio of the Jie Shou fruit membrane to water is 10:1. In some embodiments, during the extraction process, the primary extract can be further filtered to remove solids such as the seeds of the fruit after solvent extraction, resulting in a filtered solution of the fruit seeds. For example, the primary extract is filtered through a 400-mesh sieve to remove fine solids. Then, the filtered solution of the fruit seeds is concentrated under reduced pressure at 60°C ± 5°C with steam at 1 ± 0.2 kg / cm 2 until the Brix value of the solution reaches 8.8 ± 0.5, at which point the concentration is stopped to obtain a concentrated solution of the fruit seeds. If the pH value of the obtained concentrated solution of the fruit seeds is higher than 4.0, 0.23% malic acid can be added to lower the pH value to 3.2 ± 0.5 to obtain an extract of the fruit seeds. In some embodiments, the extract of the fruit seeds can be at least one embodiment or combination of the primary extract, the filtered solution of the fruit seeds, the concentrated solution of the fruit seeds, or the extract of the fruit seeds adjusted with malic acid. In some embodiments, the extract of the fruit seeds can reduce the concentration of malondialdehyde (MDA) in the blood of an organism. The concentration of MDA in the blood of an organism is a product of the peroxidation of cellular lipids by free radicals; the more MDA accumulates in the body, the poorer the body's antioxidant capacity. Specifically, the extract of the fruit seeds can reduce the blood MDA concentration, thereby enhancing the body's antioxidant capacity. Therefore, the extract of the fruit seeds is suitable for preparing a biological composition with antioxidant properties. In some embodiments, the aforementioned organism can be a human. In some embodiments, the extract of the fruit seeds can reduce ROS (reactive oxygen species) in an organism to reduce hair follicle cell damage. An appropriate level of ROS is necessary for the normal function of cells, but excessive ROS can generate oxidative stress on hair follicle cells, leading to cell damage and apoptosis, affecting the normal function of hair follicles, and thus potentially affecting the hair growth cycle and hair health. Therefore, the extract of the fruit seeds is suitable for preparing a composition for reducing hair follicle cell damage. In some embodiments, those of ordinary skill in the art can analyze the data of the MDA concentration in the blood of an organism and the reduction of ROS in the organism to draw some correlative conclusions: the extract of the fruit seeds enhances the antioxidant capacity of the organism, and the extract of the fruit seeds enhances the antioxidant capacity of hair follicle cells. In other words, the extract of the fruit seeds can be used to prepare a hair care composition for enhancing the antioxidant capacity of hair follicle cells in an organism. In some embodiments, the extract of Phyllanthus emblica fruit membrane has the function of enhancing hair follicle health. Enhancing hair follicle health is achieved by reducing hair follicle cell damage, increasing hair follicle cell proliferation, or a combination thereof. Among them, the extract of Phyllanthus emblica fruit membrane is a compound separated from the extract of Phyllanthus emblica fruit membrane, and the compound includes at least one compound of epicatechin coumarate, phloroglucinol, protocatechuic acid, or naringenin 7-O-(6”-O-p-coumaroyl)-β-D-glucoside or a combination thereof. In some embodiments, the compounds of the extract of Phyllanthus emblica fruit membrane are gallic acid, gallocatechin, epicatechin gallate, epicatechin coumarate, phloroglucinol, protocatechuic acid, or naringenin 7-O-(6”-O-p-coumaroyl)-β-D-glucoside. Since the causes of hair loss include internal and external factors. External factors include lifestyle factors such as stress, irregular daily routines, drug effects, diseases such as thyroid problems, and dietary environment factors such as nutritional disorders or insufficient intake. Even hair styling behaviors such as perming and dyeing may also cause hair problems. Internal factors mainly include genetic genes, the influence of dihydrotestosterone (DHT), and local microcirculation problems. Genetic genes make the scalp hair follicles more sensitive to hormones, and DHT may cause hair loss in some hair follicles. At the same time, poor blood circulation may also lead to insufficient hair nutrition and hair loss. In some embodiments, the extract of Phyllanthus emblica fruit membrane has the function of enhancing hair follicle health to improve hair loss caused by internal factors. For example, enhancing hair follicle health is to reduce hair follicle cell damage, increase hair follicle cell proliferation, increase hair follicle angiogenesis, promote the expression of hair growth genes, or reduce the expression of hair loss genes, one or a combination thereof. Therefore, the extract of Phyllanthus emblica fruit membrane is suitable for preparing a composition for hair care. In some embodiments, the extract of Phyllanthus emblica fruit membrane can effectively increase the expression of angiogenesis genes in hair follicle cells, and the angiogenesis genes can be VEGF genes (vascular endothelial growth factor). The protein encoded by the VEGF gene can bind to the receptors (VEGF receptor, VEGFR) on the surface of vascular endothelial cells, and then activate various signal transduction pathways in the cell, including but not limited to the PI3K-Akt pathway, the Ras-Raf-MEK-ERK pathway, and the Src kinase pathway, etc., thereby promoting the process of angiogenesis, and then achieving the effect of promoting the transport of hair growth nutrients. Specifically, because the health and vitality of hair follicle cells are closely related to the supply of surrounding blood vessels, and the expression of angiogenesis genes is related to the formation and proliferation of blood vessels. The extract of Phyllanthus emblica fruit membrane has the ability to increase the expression level of VEGF genes by about 1.26 times, thereby increasing the expression of angiogenesis-related genes in hair follicle cells, promoting the proliferation and development of surrounding blood vessels, improving the transport capacity of blood vessels for nutrients, enabling hair follicle cells to obtain more nutrients, and promoting hair growth and health. Therefore, the extract of Phyllanthus emblica fruit membrane is suitable for preparing a composition for hair care that increases hair follicle angiogenesis. In some embodiments, the Jie Shou fruit membrane extract can effectively enhance the expression of angiogenesis genes in hair follicle cells, and the angiogenesis genes can be IGF-1 genes. The protein encoded by the IGF-1 gene can bind to the receptors (IGF-1R (Insulin-like Growth Factor 1 Receptor) or IR (Insulin Receptor)) on the surface of vascular endothelial cells, and then activate various signal transduction pathways in the cell, including but not limited to the PI3K-Akt pathway, the Ras-Raf-MEK-ERK pathway, and the Src kinase pathway, etc., thereby promoting the process of angiogenesis, and further achieving the effect of promoting the transport of hair growth nutrients. Specifically, because the health and vitality of hair follicle cells are closely related to the supply of surrounding blood vessels, and the expression of angiogenesis genes is related to vasodilation and vascular resistance. The Jie Shou fruit membrane extract has the ability to increase the expression level of the IGF-1 gene by about 1.5 times, thereby enhancing the expression of angiogenesis-related genes in hair follicle cells, promoting the dilation of surrounding blood vessels, reducing vascular resistance, improving the transport capacity of blood vessels for nutrients, enabling hair follicle cells to obtain more nutrients, and promoting hair growth and health. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition that increases angiogenesis in hair follicles. In some embodiments, the Jie Shou fruit membrane extract can effectively promote the expression of hair growth genes, and the hair growth genes can be KROX20 genes. The protein encoded by the KROX20 gene regulates the process of hair follicle development, including but not limited to regulating the hair cycle, controlling hair pigmentation, and promoting hair follicle formation. The Jie Shou fruit membrane extract has the ability to increase the expression level of the KROX20 gene by about 1.93 times, thereby enhancing the expression of hair growth-related genes in hair follicle cells. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition that promotes hair growth genes. In some embodiments, the Jie Shou fruit membrane extract can effectively promote the expression of hair growth genes, and the hair growth genes can be SCF genes. The protein encoded by the SCF gene is a cytokine called Stem Cell Factor (abbreviated as SCF) . SCF plays an important physiological role in multiple tissues and organs, including the regulation of hair growth and hair follicle function. The Jie Shou fruit membrane extract has the ability to increase the expression level of the SCF gene by about 1.08 times, thereby enhancing the expression of hair growth-related genes in hair follicle cells. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition that promotes hair growth genes. In some embodiments, the Jie Shou fruit membrane extract can effectively inhibit hair loss gene expression, and the hair loss gene can be SRD5α1, SRD5α2, or a combination thereof. The 5α-reductase encoded by the SRD5α1 gene and the SRD5α2 gene participates in the metabolic process of androgens, including the conversion of testosterone into more active dihydrotestosterone; excessive dihydrotestosterone is related to androgen-related intrinsic factor hair loss (such as male pattern baldness). Since the effect of dihydrotestosterone on hair follicles may lead to apoptosis of hair and shortening of the growth cycle, thereby inhibiting hair follicle hair growth, it will also promote sebum secretion by sebaceous glands, resulting in hair loss and oily scalp. The Jie Shou fruit membrane extract has the ability to inhibit the SRD5α1 gene by about 0.75 times and the ability to inhibit the SRD5α2 gene by about 0.68 times, thereby reducing hair loss gene expression to increase hair follicle health. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition for reducing hair loss genes. In some embodiments, the Jie Shou fruit membrane extract can effectively inhibit hair loss gene expression, and the hair loss gene can be AR (Androgen receptor). After highly active AR binds to excessive dihydrotestosterone, it may exacerbate the effect of androgens on hair follicles, resulting in inhibited hair follicle hair growth, thereby inhibiting hair follicle hair growth. The Jie Shou fruit membrane extract has the ability to inhibit the AR gene by about 0.8 times, thereby reducing hair loss gene expression to increase hair follicle health. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition for reducing hair loss genes. In some embodiments, the Jie Shou fruit membrane extract can effectively inhibit hair loss gene expression, and the hair loss gene can be the TGF-β gene (encoding transforming growth factor-β). The TGF-β gene inhibits hair growth, promotes inflammation, and causes hair loss and scalp inflammation under certain conditions, and the certain conditions may include but are not limited to changes in androgen levels. The Jie Shou fruit membrane extract has the ability to inhibit the TGF-β gene by about 0.87 times, thereby reducing hair loss gene expression to increase hair follicle health. Therefore, the Jie Shou fruit membrane extract is suitable for preparing a hair care composition for reducing hair loss genes. In some embodiments, the Jie Shou fruit membrane extract is used to prepare a composition for hair health. For example, the composition can be in the form of a capsule containing a powder of the Jie Shou fruit membrane extract, or a beverage containing a liquid Jie Shou fruit membrane extract. In some embodiments, a composition comprises a container and the Jie Shou fruit membrane extract contained in the container. For example, in the Jie Shou fruit membrane extract beverage, the container contains a Jie Shou fruit beverage containing the Jie Shou fruit membrane extract. In some embodiments, when the aforementioned composition can be manufactured into dosage forms suitable for enteral, parenteral, or oral administration using techniques well known to those skilled in the art. In some embodiments, enteral or oral administration can be, but is not limited to, tablets, troches, lozenges, pills, capsules, dispersible powders, granules, solutions, suspensions, emulsions, syrups, elixirs, slurries, or the like. In some embodiments, when the aforementioned composition is an edible composition, the edible composition contains a specific content of the extract of the pericarp of Phyllanthus niruri Linn. wherein, the form of the edible composition can be powder, granule, solution, colloid, or paste. In some embodiments, the edible composition containing the extract of the pericarp of Phyllanthus niruri Linn. can be a food product or a food additive. In some embodiments, the edible composition containing the extract of the pericarp of Phyllanthus niruri Linn. can be beverages, fermented foods, bakery products, health foods, or dietary supplements, etc. In some embodiments, the edible composition containing the extract of the pericarp of Phyllanthus niruri Linn. may further include an adjuvant. For example, the adjuvant can be Maltodextrin, malic acid, sucralose, citric acid, fruit flavor, honey flavor, steviol glycoside, or a combination thereof, etc. The type and quantity of the selected adjuvant fall within the professional knowledge and routine techniques of those skilled in the art. In some embodiments, the food additive can be a flavoring agent, a sweetening agent, a spice, a pH adjuster, an emulsifier, a coloring agent, or a stabilizer, etc. In some embodiments, in the aforementioned composition, the daily intake of the extract of the pericarp of Phyllanthus niruri Linn. is 4 grams in liquid form. Unless otherwise specified in the following examples, the experimental procedures are carried out at room temperature (25°C - 30°C) and normal pressure (1 atm). And in the following examples, Excel software is used for statistical analysis. The data are expressed as mean ± standard deviation (SD), and the differences between groups are analyzed by student's t-test. In the figures, "*" represents a p-value less than 0.05, "**" represents a p-value less than 0.01, and "***" represents a p-value less than 0.001. The more "*" there are, the more significant the statistical difference is. Example 1: Preparation of the extract of the pericarp of Phyllanthus niruri Linn. A. Materials: 1. Pericarp of Phyllanthus niruri Linn. (origin: Vietnam). 2. Secondary water, also known as RO water (Reverse Osmosis) or double-distilled water, hereinafter simply referred to as "water". B. Preparation process: 1. Heat the water to 85 ± 5 °C, then add the Jieshou fruit membrane and soak the Jieshou fruit membrane in the water at 85 ± 5 °C for 90 minutes to form a primary extract containing solids. Here, the weight ratio of the added Jieshou fruit membrane to the water is 1:10. 2. Filter the cooled primary extract through a 400-mesh filter to remove solids (i.e., the Jieshou fruit membrane after extraction) to obtain the Jieshou fruit membrane filtrate. 3. After setting the temperature of the concentrator (model: Rotavapor R-100; brand: BUCHI) to 60 ± 5 °C, use the concentrator to perform vacuum concentration on the filtrate. Stop the concentration when the Brix value of the filtrate is concentrated to 8.8 ± 0.5 °Bx to obtain the Jieshou fruit membrane concentrate. 4. Add 0.23% malic acid to the Jieshou fruit membrane concentrate, adjust the pH value of the Jieshou fruit membrane concentrate to pH 3.2 ± 0.5, and then obtain the Jieshou fruit membrane extract. Example 2 : Compound analysis test A. Materials and instruments: 1. Nuclear Magnetic Resonance Spectrometer (NMR): 1D and 2D spectra use Ascend 400 MHz, purchased from Bruker Co., Germany. The chemical shift is represented by δ, and the unit is ppm. 2. High-resolution liquid chromatography-mass spectrometer: Ultra-high performance liquid chromatography (Ultimate 3000 HPLC, purchased from Thermo Fisher Scientific) and high-resolution orbitrap ion trap mass spectrometer (Q-EXACTIVE System with Ion Max Source, purchased from Thermo Fisher Scientific) are used for measurement, and the unit is m / z. 3. Medium pressure liquid chromatography (MPLC): CombiFlash® Rf+, purchased from Teledyne ISCO, Lincoln, Nebraska, USA. 4. High Performance Liquid Chromatography (HPLC): The high performance liquid chromatography (HPLC) is of the Hitachi chromaster 5260 series; the eluent delivery system is Hitachi chromaster 5110; the column thermostat is Hitachi chromaster 5310; the diode array detector (DAD) is Hitachi chromaster 5430, and the detection wavelength is 280 nm. 5. HPLC analytical column: Mightysil RP-18 GP 250 (250 x 4.6 mm, 5μm, purchased from Kanto, Tokyo, Japan). 6. Packing materials for column chromatography: (1) Macroporous resin: Diaion HP-20, purchased from Mitsubishi Chemical Corporation, Japan. (2) Normal-phase silica gel: Merck Kieselgel 60, 40 - 63 um, purchased from Merck, Germany, product number Art. 9385. (3) Reverse-phase silica gel: Merck LiChroprep® RP-18, 40 - 63 um, purchased from Merck, Germany, product number Art. 0250. 7. Thin-Layer Chromatography: (1) Normal-phase TLC aluminum sheet: Thin-layer chromatography sheet coated with silica gel 60 F 254 (0.25 mm), purchased from Merck, Germany. (2) Reverse-phase TLC aluminum sheet: Thin-layer chromatography sheet coated with RP-18 F 254-S (0.25 mm), purchased from Merck, Germany. 8. UV Lamp: UVP UVGL-25, with wavelengths of 254 nm and 365 nm. 9. Solvents: Methanol, n-butanol, methanol-d4 (degree of deuteration 99.5%), heavy water (deuterium oxide, degree of deuteration > 99.8%), and dimethyl sulfoxide-d6 (degree of deuteration > 99.9%), all purchased from Merck, Taiwan. 10. Filter membrane: Polyvinylidene fluoride membrane filters (PVDF), with a pore size of 0.22 microns, purchased from Millipore, USA. 11. Sample: The Jie Shou fruit membrane extract prepared in Example 1. B. Compound separation and structure identification process: 1. Using n-butanol as a solvent, 5 liters of the Jie Shou fruit membrane extract prepared in Example 1 was subjected to liquid-liquid partition extraction to obtain 48.9 g of the n-butanol-soluble part (lipophilic components) and 156.3 g of the water-soluble part (water-soluble components), thereby separating the lipophilic components and water-soluble components of the Jie Shou fruit membrane extract obtained in Example 1. During the separation and purification process, a bioassay-guided fractionation method was used. 2. Take 40 g of the n-butanol-soluble part from Step 1 and continue to perform preliminary separation by column chromatography using Diaion HP-20 macroporous resin. Sequentially, 5 liters each of pure water, a 1:1 volume ratio of pure water-methanol, and methanol were used as eluents to obtain 3 separated parts (hereinafter referred to as the first separated part P1, the second separated part P2, and the third separated part P3). 3. Take the first separated part P1 and re-separate it using a reverse-phase medium-pressure liquid chromatograph (RP-MPLC) to obtain various eluates. Here, the eluent was linearly eluted from water to methanol for 60 minutes at a flow rate of 15 ml per minute. Subsequently, reverse-phase thin-layer chromatography (reverse-phase TLC aluminum sheet: thin-layer chromatography sheet, coated with RP-18 F 254-S (0.25 mm)) was used to combine eluates with similar results to obtain 3 sub-separated parts, as shown in Figure 1 (hereinafter referred to as the fourth separated part P4, the fifth separated part P5, and the sixth separated part P6). 4. The fifth separated part P5 was purified by reverse-phase high-performance liquid chromatography (methanol / water = 1 / 19) to obtain compound TCI-CsN-01. After analyzing its chemical structure by hydrogen nuclear magnetic resonance spectroscopy ( 1 H-NMR), it was confirmed that compound TCI-CsN-01 is gallic acid, as shown in Figure 2. 5. The sixth separation fraction P6 was purified by reverse-phase high-performance liquid chromatography (methanol / water = 1 / 9) to obtain compounds TCI-CsN-11 and TCI-CsN-12. It was confirmed that compound TCI-CsN-11 was phloroglucinol and TCI-CsN-12 was protocatechuic acid, as shown in Figures 6 and 7. 6. Another second separation fraction P2 was re-separated using a reverse-phase medium-pressure liquid chromatograph (RP-MPLC) to obtain various eluates. Here, the eluent was linearly eluted from water to methanol for 100 minutes at a flow rate of 15 mL per minute. Subsequently, reverse-phase thin-layer chromatography was used to combine the eluates with similar results, yielding 8 sub-separation fractions, as shown in Figure 1 (hereinafter referred to as the seventh separation fraction P7, eighth separation fraction P8, ninth separation fraction P9, tenth separation fraction P10, eleventh separation fraction P11, twelfth separation fraction P12, thirteenth separation fraction P13, and fourteenth separation fraction P14). 7. The seventh separation fraction P7 was purified by reverse-phase HPLC (methanol / water = 3 / 17) to obtain compound TCI-CsN-02. After analyzing its chemical structure by hydrogen nuclear magnetic resonance spectroscopy ( 1 1H-NMR), it was confirmed that compound TCI-CsN-02 was gallocatechin, as shown in Figure 3. 8. The tenth separation fraction P10 was purified by reverse-phase HPLC (methanol / water = 3 / 7) to obtain compound TCI-CsN-07. It was confirmed that compound TCI-CsN-07 was epicatechin gallate, as shown in Figure 4. 9. The eleventh separation fraction P11 was purified by reverse-phase HPLC (methanol / water = 2 / 3) to obtain compound TCI-CsN-13. TCI-CsN-13 was naringenin 7-O-(6”-O-p-coumaryl)-β-D-glucoside, as shown in Figure 8. 10. The thirteenth separation fraction P13 was purified by reverse-phase HPLC (methanol / water = 1 / 1) to obtain compound TCI-CsN-05. After analyzing its chemical structure by hydrogen nuclear magnetic resonance spectroscopy ( 1 1H-NMR) and comparing with the literature, it was confirmed that compound TCI-CsN-05 was epicatechin coumarate, as shown in Figure 5. The names and chemical structural formulas of the aforementioned compounds are shown in Table 1 below. Table 1 Example 3: Antioxidant Test of Hair Follicle Cells A. Materials and Instruments: 1. Cell line: Human Follicle Dermal Papilla Cells (HFDPC), purchased from PromoCell, cell number C-12071, hereinafter referred to as HFDPC cells for short. 2. Cell culture medium: Follicle Dermal Papilla Cell Growth Medium (purchased from PromoCell, product number C-26501). 3. DCFH-DA working reagent: Prepared from DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate) (purchased from Sigma, product number SI-D6883-50MG) and DMSO (purchased from Sigma, product number 472301). 4. Preparation of 1X DPBS: Dilute 10X DPBS 10-fold with sterilized ddH 2 O. 5. 100 mM H 2 O 2 Aqueous solution: Mix H 2 O 2 and 1X DPBS in a volume ratio of 1:88 and mix well for later use. 6. Trypsin: Prepared by diluting 10X trypsin (purchased from Gibco, product number 15400-054) with 9 volumes of its DPBS. 7. Flow cytometer, model BD Accuri, purchased from BD. B. Test Procedure: 1. Seed HFDPC cells at a density of 1 x 10 5 per well into a 6-well culture plate containing 2 ml of cell culture medium per well, and culture at 37°C for 24 hours. Here, the HFDPC cells are divided into three test groups, namely: blank group G01, control group G02, and experimental group. 2. After 24 hours of incubation, the culture medium of each group was replaced with the experimental medium. Among them, the experimental media for the blank group G01 and the control group G02 were cell culture media without the extract of the pericarp of Phyllanthus emblica Linn. or its compounds, and the experimental media for the experimental groups are shown in Tables 2 and 3. Then, each group was treated at 34 °C for 1 hour. Table 2 Table 3 3. After 1 hour of treatment, the DCFH-DA reagent was added to each group to a final concentration of 5 μg / mL, and each group was treated at 37 °C for 15 minutes. 4. After 15 minutes of treatment, 5 μl / well of 100 mM H 2 O 2 aqueous solution was added, and the treatment was carried out at 34 o °C for 0.5 hour. 5. The experimental media of each group after treatment were removed, and the cells were rinsed twice with 1X DPBS. 6. After rinsing, 500 μl of trypsin was added to each well and reacted in the dark for 5 minutes. After the reaction, cell culture medium was added to terminate the reaction. Then, the suspended cells and cell culture medium in each well were collected into the corresponding 1.5 ml microcentrifuge tube, and the cells in each centrifuge tube were precipitated by centrifugation at 400 xg for 5 minutes. 7. The supernatant in each centrifuge tube of each group was removed, and the precipitated cells were washed once with 1X DPBS and then centrifuged at 400 xg for 5 minutes. The supernatant in each centrifuge tube of each group was removed again, and 200 μl of 1X DPBS was added to each centrifuge tube to resuspend the cells to form a cell suspension. 8. After setting the parameters of the excitation light of the flow cytometer to 450 - 490 nm and the parameters of the scattered light to 510 - 550 nm, the fluorescence signal of DCFH-DA in each group was detected using a flow cytometer. Since DCFH-DA will be hydrolyzed to DCFH (dichlorodihydrofluorescein) first after entering HFDPC cells and then oxidized to DCF (dichlorofluorescein) that can emit green fluorescence by reactive oxygen species (ROS), the fluorescence intensity of HFDPC cells treated with DCFH-DA can reflect the ROS content in CCD-996SK cells, and thereby the proportion of the number of cells with highly expressed ROS in HFDPC cells to the original number of cells can be known. C. Test results: The relative ROS production of all groups was calculated according to the following formula: Relative ROS production (%) = (green fluorescence signal of each group / green fluorescence signal of blank group G01) × 100%. Since the experiment was performed in triplicate, the measurement results of the triplicate experiments of each group were averaged to obtain the average value. Then, the statistical significant differences between the experimental results of control group G02 and other groups were obtained by statistical analysis of student’s t-test. In the figure, "*" represents that the p-value is less than 0.05 compared with control group G02, "**" represents that the p-value is less than 0.01 compared with control group G02, and "***" represents that the p-value is less than 0.001 compared with control group G02. Please refer to Figure 9. The cells of blank group G01 were not treated with the extract of Jie Shou fruit membrane and did not add H 2 O 2 stimulation. Therefore, the experimental results of blank group G01 represent the performance of cells under normal physiological metabolism. Here, when setting the relative ROS production of blank group G01 as 100%, the relative ROS production of control group G02 was 180.7%; the relative ROS production of experimental group G30 was 65.8%, and the relative ROS production of experimental group G32 was 53.5%. That is to say, compared with blank group G01, the relative ROS production of cells in control group G02 increased by about 80.1% after adding H 2 O 2 stimulation. Compared with control group G02, after adding a lower concentration of the extract of Jie Shou fruit membrane in experimental group G30 and then adding H 2 O 2 stimulation, the relative ROS production decreased significantly by about 63.5%. And compared with blank group G01, the relative ROS production of the experimental group decreased by about 34.2% (the ROS inhibition rate is shown in Table 4 below). Compared with control group G02, after adding a higher concentration of the extract of Jie Shou fruit membrane in experimental group G32 and then adding H 2 O 2 stimulation, the relative ROS production decreased significantly by about 70.4%. And compared with blank group G01, the relative ROS production of experimental group 1 decreased by about 46.5% (the ROS inhibition rate is shown in Table 4 below). Table 4 Please refer to Figure 10. The cells of the blank group G01 were not treated with the extract of the Jieshou fruit membrane and did not add H 2 O 2 stimulation. Therefore, the test results of the blank group G01 represent the performance of cells under normal physiological metabolism. The relative ROS production of each group is shown in Figure 10. Here, the ROS inhibition rate (%) of each group is calculated by the formula: (ROS production of control group G02 - ROS production of blank group G01) - (ROS production of experimental group - ROS production of blank group G01) ÷ (ROS production of control group G02 - ROS production of blank group G01). The results of the ROS inhibition rate of the experimental group are shown in Table 5 below. Table 5 It can be seen from this that the extract of the Jieshou fruit membrane can significantly reduce H 2 O 2 induced ROS production in hair follicle cells. H 2 O 2 will induce cells to perform aerobic metabolism and thus produce ROS. ROS in cells will attack macromolecules such as proteins, nucleic acids, and lipids, causing cell damage. In other words, it can be proven by experiments that the extract of the Jieshou fruit membrane and its compounds have the ability to enhance the antioxidant capacity of hair follicle cells and reduce the damage of hair follicle cells. The extract of the Jieshou fruit membrane has the effect of enhancing antioxidant activity and reducing cell damage. Example 4: Promotion of hair follicle cell proliferation test A. Materials and instruments: 1. Detection kit: Cell proliferation detection reagent kit (Click-iT™ Plus EdU Flow Cytometry Assay Kits - Alexa Fluor™ 488 picolyl azide, 50 tests), purchased from Invitrogen, product number C10632. 2. Flow cytometer (purchased from BD, product number BD Accuri). B. Test procedure: 1. Inoculate HFDPC cells at a density of 1×10 5 per well into a 6-well culture plate and place it in 5% CO 2, Cultivate at 37 °C until the cell confluence reaches 80-90%. Here, the HFDPC cells are divided into each group as shown in Table 6 and Table 7. Table 6 Table 7 2. After culturing for 24 hours, use a cell proliferation detection kit to measure the DNA content of each group. Here, after processing the cells of each group according to the experimental procedures provided by the cell proliferation detection kit, set the parameters of the excitation light of the flow cytometer to 450-490 nm and the parameters of the scattered light to 510-550 nm, and then use the flow cytometer to detect the fluorescence signal of EdU (5-ethynyl-2'-deoxyuridine) in each group. C. Test results: Use the DNA content of each group as the basis for analyzing the hair follicle cell proliferation rate of each group. The relative hair follicle cell proliferation rate of all groups is calculated according to the following formula: Relative hair follicle cell proliferation rate (%) = (fluorescence signal of each group / fluorescence signal of blank group G01) × 100%. The statistical significant differences between the blank group G01 and the test results of other groups are obtained by statistical analysis of the student's t-test. In the figure, "*" represents that the p-value is less than 0.05 compared with the blank group G01, "**" represents that the p-value is less than 0.01 compared with the blank group G01, and "***" represents that the p-value is less than 0.001 compared with the blank group G01. Please refer to Figure 11. The blank group G01 was not treated with the extract of Jie Shou Guo membrane, so the test results of the blank group G01 represent the performance of HFDPC cells under normal physiological metabolism. Here, when setting the relative hair follicle cell proliferation rate of the blank group G01 to 100%, the relative hair follicle cell proliferation rate of the control group G02 is 138.8%; the relative hair follicle cell proliferation rate of the experimental group G30 is 114.6%; the relative hair follicle cell proliferation rate of the experimental group G32 is 86.9%. That is to say, compared with the blank group G01, the HFDPC cells of the control group G02 significantly increased the relative hair follicle cell proliferation rate by about 38.8% after adding 20% fetal bovine serum. Compared with the blank group G01, the HFDPC cells of the experimental group G30 significantly increased the relative hair follicle cell proliferation rate by about 14.6% after adding 0.125% extract of Jie Shou Guo membrane. Please refer to Figure 12. The blank group G01 was not treated with the extract of the fruit membrane of Jieshou, so the test results of the blank group G01 represent the performance of HFDPC cells under normal physiological metabolism. Here, with the relative hair follicle cell proliferation rate of the blank group G01 set at 100%, the relative hair follicle cell proliferation rate of the control group G02 was 118.8%; the relative hair follicle cell proliferation rate of the experimental group G34 was 83.7%; the relative hair follicle cell proliferation rate of the experimental group G36 was 93.5%; the relative hair follicle cell proliferation rate of the experimental group G38 was 89.9%; the relative hair follicle cell proliferation rate of the experimental group G40 was 114.1%; the relative hair follicle cell proliferation rate of the experimental group G42 was 127.9%; the relative hair follicle cell proliferation rate of the experimental group G44 was 109.6%; the relative hair follicle cell proliferation rate of the experimental group G46 was 125%. That is to say, relative to the blank group G01, after adding 20% fetal bovine serum, the HFDPC cells in the control group G02 significantly increased the relative hair follicle cell proliferation rate by about 18.8%. Relative to the blank group G01, there was no significant difference in the experimental groups G34 - G38; the experimental groups G40 - G46 had a significant increase in the relative hair follicle cell proliferation rate of about 14.1%, 27.9%, 9.6% and 25%. From the experimental results, it can be seen that 0.125% extract of the fruit membrane of Jieshou, and the experimental groups G40 - G46 can significantly promote the proliferation of hair follicle cells. Moreover, the effects of the experimental groups G42 and G46 in promoting the proliferation rate of hair follicle cells are better than those of the control group G02. In other words, it can be proved by the experiment that the extract of the fruit membrane of Jieshou and its compounds can promote the proliferation of hair follicle cells, promote hair growth, and thus be used for hair care. Example 5 : Test on the expression of genes related to hair care by the extract of the fruit membrane of Jieshou A. Materials and Instruments: 1. RNA extraction reagent kit, purchased from Genemark Company. 2. SuperScript® III reverse transcriptase, purchased from Invitrogene Company. 3. ABI StepOnePlusTM Real-Time PCR system, purchased from Thermo Fisher Scientific Company. 4. KAPA SYBR FAST qPCR Master Mix (2X) Kit, purchased from KAPA Biosystems Company, product number KK4600. B. Test Procedure: Seed HFDPC cells at 1×10 per well 5The cells were inoculated into a 6-well culture dish containing 2 mL of culture medium per well at an individual density and cultured at 37°C for 24 hours. After culturing, the HFDPC cells were divided into several blank groups G01 and experimental groups to compare different gene expressions. Among them, the culture medium of the blank group G01 did not contain any extract, and the culture media of the experimental groups G30 and G32 contained 0.125% or 0.25% of the Jie Shou fruit membrane extract prepared in Example 1. Then, they were cultured at 37°C for 6 hours, 24 hours, and 48 hours. The culture media of the blank group G01 and the experimental groups with different concentrations after culturing were removed, and the cells were rinsed with PBS. After rinsing, the cell membranes of the HFDPC cells in each group were disrupted with the cell lysate of the RNA extraction reagent kit to form cell solutions. The RNA extraction reagent kit was used to extract the RNA in the cell solutions of each group. 2000 nanograms (ng) of the extracted RNA from each group was used as a template, and the extracted RNA was reverse transcribed into the corresponding cDNA by SuperScript® III reverse transcriptase. Use ABI StepOnePlus TM Real-Time PCR system, and quantitative real-time reverse transcription polymerase chain reaction was performed on the cDNA using KAPA SYBR FAST qPCR Master Mix (2X) Kit and the primer combinations in Table 8 to observe the expression levels and melting curves of various target genes in the HFDPC cells of the blank group G01 and the experimental groups. The instrument settings for the quantitative real-time reverse transcription polymerase chain reaction were 95°C for 20 seconds, 95°C for 3 seconds, 60°C for 30 seconds, and repeated for 40 cycles. Use 2 - ΔΔ Ct The method was used to measure the relative expression levels of the target genes. The relative expression level was defined as the fold change of the RNA expression level of the target gene in the experimental group relative to the RNA expression level of the same gene in the blank group G01. 2 - ΔΔ Ct The method uses the cycle threshold of the TBP gene as the cycle threshold (Ct) of the reference gene for internal control, and calculates the fold change according to the following formula: △Ct = Ct 實驗組之目標基因 / 空白組 G01 之目標基因 - Ct GAPDH △△Ct = △Ct 實驗組之目標基因 - △Ct 空白組 G01 之目標基因 Fold change = 2 - ΔΔ Ct 平均值 The standard deviation of relative expression levels was calculated by STDEV in Excel. The statistical significant differences between the experimental results of the blank group G01 and other groups were obtained by statistical analysis of the student's t-test. In the figures, "*" represents that the p-value is less than 0.05 compared with the blank group G01, "**" represents that the p-value is less than 0.01 compared with the blank group G01, and "***" represents that the p-value is less than 0.001 compared with the blank group G01. Table 8 below shows the relevant sequences for the gene expression experiments. Table 8 Here, the statistical significant differences between the measurement results of the blank group G01 and the experimental groups were obtained by statistical analysis of the student's t-test. (In the figures, "*" represents that the p-value is less than 0.05 compared with the blank group G01, "**" represents that the p-value is less than 0.01 compared with the blank group G01, and "***" represents that the p-value is less than 0.001 compared with the blank group G01. The more "*", the more significant the statistical difference). Please refer to Figures 13 and 14 for the gene test results after 6 hours of culture. The HFDPC cells in the blank group G01 were not treated with anything, which means that under normal physiological metabolism conditions, the gene expression level was set to 1.0. The expression levels of the SRD5α1 gene in the SRD5α1 gene experimental groups were -0.49 times and -0.54 times that of the blank group G01, respectively. The expression levels of the SRD5α2 gene in the SRD5α2 gene experimental groups were 3.4 times and 3.65 times that of the blank group G01. The expression levels of the AR gene in the AR gene experimental groups were 1.04 times and 1.3 times that of the blank group G01. The expression levels of the KROX20 gene in the KROX20 gene experimental groups were 1.93 times and 1.85 times that of the blank group G01. The expression levels of the SCF gene in the SCF gene experimental groups were 1.08 times and 1.05 times that of the blank group G01. The expression levels of the VEGF gene in the VEGF gene experimental groups were 1.16 times and 1.26 times that of the blank group G01. The expression levels of the IGF1 gene in the IGF1 gene experimental groups were 1.49 times and 1.5 times that of the blank group G01. The expression levels of the TGF-β gene in the TGF-β gene experimental groups were -0.87 times and -0.85 times that of the blank group G01. Please refer to Figures 15 and 16 for the gene test results after 24 hours of culture. The HFDPC cells in the blank group G01 were not treated with anything, which means that under normal physiological metabolism conditions, the gene expression level was set to 1.0. The expression levels of the SRD5α1 gene in the SRD5α1 gene experimental groups were -0.73 times and -0.75 times that of the blank group G01, respectively. The expression levels of the SRD5α2 gene in the SRD5α2 gene experimental groups were 1.02 times and 1.12 times that of the blank group G01. The expression levels of the AR gene in the AR gene experimental groups were 1.29 times and 1.43 times that of the blank group G01. The expression levels of the KROX20 gene in the KROX20 gene experimental groups were 1.01 times and 1.04 times that of the blank group G01. The expression levels of the SCF gene in the SCF gene experimental groups were -0.73 times and -0.665 times that of the blank group G01. The expression levels of the VEGF gene in the VEGF gene experimental groups were -0.89 times and -0.93 times that of the blank group G01. The expression levels of the IGF1 gene in the IGF1 gene experimental groups were -0.76 times and -0.7 times that of the blank group G01. The expression levels of the TGF-β gene in the TGF-β gene experimental groups were -0.76 times and -0.7 times that of the blank group G01. Please refer to Figures 17 and 18 for the gene test results after 48 hours of culture. In the HFDPC cells of the blank group G01, no treatment was performed, which means that under normal physiological metabolism, the gene expression level was set to 1.0. The expression levels of the SRD5α1 gene in the SRD5α1 gene experimental groups were -0.75 times and -0.76 times that of the blank group G01, respectively. The expression levels of the SRD5α2 gene in the SRD5α2 gene experimental groups were 1.2 times and -0.68 times that of the blank group G01. The expression levels of the AR gene in the AR gene experimental groups were -0.8 times and -0.77 times that of the blank group G01. The expression levels of the KROX20 gene in the KROX20 gene experimental groups were -0.98 times and -0.93 times that of the blank group G01. The expression levels of the SCF gene in the SCF gene experimental groups were -0.6 times and -0.68 times that of the blank group G01. The expression levels of the VEGF gene in the VEGF gene experimental groups were -0.77 times and -0.91 times that of the blank group G01. The expression levels of the IGF1 gene in the IGF1 gene experimental groups were -0.72 times and -0.61 times that of the blank group G01. The expression levels of the TGF-β gene in the TGF-β gene experimental groups were -0.8 times and -0.78 times that of the blank group G01. The statistical differences in hair care-related genes compared to the blank group G01 are shown in Table 9 below. Table 9 Note: SRD5α1, SRD5α2, AR, and TGF-β are hair loss-related genes; KROX20 and SCF are hair growth-related genes; VEGF and IGF1 are angiogenesis-related genes. It can be seen from this that the extract of Jie Shou fruit membrane can significantly inhibit the expression levels of the SRD5α1 gene, SRD5α2 gene, AR gene, and TGF-β gene; significantly increase the expression levels of the KROX20 gene and SCF gene; and significantly increase the expression levels of the VEGF gene and IGF1 gene, indicating that the extract of Jie Shou fruit membrane increases hair follicle angiogenesis, promotes the delivery of hair growth nutrients, enhances hair follicle formation, and thus improves hair follicle health, and can reduce scalp oiliness, reduce hair loss, reduce inflammation, and reduce hair loss caused by scalp inflammation, so as to improve hair follicle health. Example 6: Human trial - Blood test A. Test procedure: Let 7 healthy adult subjects aged 20 or above who have not taken any oral or topical anti - hair - loss drugs or health products and who are aware of hair - loss problems take one 50 - milliliter bottle of the test drink daily for 12 consecutive weeks (i.e., 84 days). The test drink contains 4 milliliters of the Jie - Shou - Guo - Membrane extract prepared in Example 1, and the rest is water. Also, the subjects have their blood drawn at week 0, week 8, and week 12 to detect the change in the concentration of malondialdehyde, an antioxidant index in the blood, before and after taking the Jie - Shou - Guo - Membrane extract. In this example, the detection of the concentration of malondialdehyde, an antioxidant index in the subjects' blood, was outsourced to a testing unit, and the external testing unit referred to the blood - test standards announced by the Ministry of Health and Welfare. B. Test results: The statistical significant differences among the test results at week 0, week 8, and week 12 were obtained through statistical analysis using the student's t - test. In the figure, "*" represents a p - value less than 0.05 compared with week 0, "**" represents a p - value less than 0.01 compared with week 0, and "***" represents a p - value less than 0.001 compared with week 0. Please refer to Figure 19. The concentration of malondialdehyde, an antioxidant index in the subjects' blood, at week 0 was approximately 1.47 μg / mL. The concentration of malondialdehyde in the subjects' blood at week 8 (i.e., after taking the Jie - Shou - Guo - Membrane extract for 8 weeks) significantly decreased to approximately 1.31 μg / mL. The concentration of malondialdehyde in the subjects' blood at week 12 (i.e., after taking the Jie - Shou - Guo - Membrane extract for 12 weeks) decreased to approximately 0.95 μg / mL. In other words, compared with before taking, after continuously taking the Jie - Shou - Guo - Membrane extract for 8 weeks, the concentration of malondialdehyde in the blood of these subjects decreased by 11.4%; after continuously taking the Jie - Shou - Guo - Membrane extract for 12 weeks, the concentration of malondialdehyde in the blood of these subjects decreased by 35.6%. And the proportion of improved subjects reached 100%. This indicates that the Jie - Shou - Guo - Membrane extract can reduce the blood MDA concentration, enhance the antioxidant capacity in the body, thereby reducing follicular cell damage and improving follicular health. Example 7: Human trial - Hair diameter detection A. Test procedure: Let 7 healthy adult subjects aged 20 or above who have not taken any oral or topical anti - hair - loss drugs or health products and who are aware of hair - loss problems take one 50 - milliliter bottle of the test drink daily for 12 consecutive weeks (i.e., 84 days). The test drink contains 4 milliliters of the Jie - Shou - Guo - Membrane extract prepared in Example 1, and the rest is water. Also, the subjects have their hair diameters detected at week 0, week 8, and week 12 to detect the change in hair - diameter thickness before and after taking the Jie - Shou - Guo - Membrane extract. This example uses the inspection instrument ASG-300 / API-100 scalp detector for detection. It was purchased from Aram Huvis, South Korea, and the unit is millimeters (mm). The detection method is to calculate the diameter about 1 cm from the hair root at the same position on the top of the head, and measure the average value with 3-5 hairs each time. B. Test results: The statistically significant differences between the test results at week 0, week 8, and week 12 were obtained by statistical analysis using the student’s t-test. In the figures, "*" represents a p-value less than 0.05 compared with week 0, "**" represents a p-value less than 0.01 compared with week 0, and "***" represents a p-value less than 0.001 compared with week 0. Please refer to Figure 20. The hair diameter of the subjects at week 0 was about 0.054 mm, the hair diameter of the subjects at week 8 (i.e., after continuously taking the Jie Shou fruit membrane extract for 8 weeks) was about 0.073 mm, and the hair diameter of the subjects at week 12 (i.e., after continuously taking the Jie Shou fruit membrane extract for 12 weeks) was about 0.081 mm. In other words, compared with before taking, continuously taking the Jie Shou fruit membrane extract for 8 weeks can significantly increase the hair diameter of these subjects by 35.2%; continuously taking the Jie Shou fruit membrane extract for 12 weeks can significantly increase the hair diameter of these subjects by 50%. And the proportion of improved people reached 100%. Thus, it can be seen that the Jie Shou fruit membrane extract can indeed increase the hair diameter of the subjects, increase hair stability, and improve hair follicle health. In summary, the Jie Shou fruit membrane extract of any embodiment is obtained by extracting the membrane of the Jie Shou fruit with a solvent, and its use for preparing a hair care composition, thereby providing a composition that can produce a hair care effect on an individual when administered to the individual. In other words, the aforementioned composition has the function of hair care. In some embodiments, the composition prepared from the Jie Shou fruit membrane extract also has one or more of the following functions: improving the antioxidant capacity of hair follicle cells, increasing hair follicle angiogenesis, promoting the expression of hair growth genes, reducing the expression of hair loss genes, and increasing hair diameter. G01: Blank group G02: Control group G30~G46: Experimental groups P1~P14: Separation parts Figure 1 is the compound separation flowchart of the Jieshou fruit membrane extract. Figure 2 is the hydrogen-nuclear magnetic resonance spectrum of gallic acid. Figure 3 is the hydrogen-nuclear magnetic resonance spectrum of epigallocatechin. Figure 4 is the hydrogen-nuclear magnetic resonance spectrum of epicatechin gallate. Figure 5 is the hydrogen-nuclear magnetic resonance spectrum of epicatechin coumarate. Figure 6 is the hydrogen-nuclear magnetic resonance spectrum of phloroglucinol. Figure 7 is the hydrogen-nuclear magnetic resonance spectrum of protocatechuic acid. Figure 8 is the hydrogen-nuclear magnetic resonance spectrum of naringenin 7-O-(6”-O-p-coumaroyl)-β-D-glucoside. Figure 9 is the bar chart of the cell experiment results of the relative ROS production of the Jieshou fruit membrane extract. Figure 10 is the bar chart of the cell experiment results of the relative ROS production of the compounds of the Jieshou fruit membrane extract. Figure 11 is the bar chart of the cell experiment results of the relative cell proliferation rate of the Jieshou fruit membrane extract. Figure 12 is the bar chart of the cell experiment results of the relative cell proliferation rate of the compounds of the Jieshou fruit membrane extract. Figure 13 is the bar chart of the cell experiment results of the relative gene expression level (6 hours) of the Jieshou fruit membrane extract. Figure 14 is the bar chart of the cell experiment results of the relative gene expression level (6 hours) of the Jieshou fruit membrane extract. Figure 15 is the bar chart of the cell experiment results of the relative gene expression level (24 hours) of the Jieshou fruit membrane extract. Figure 16 is the bar chart of the cell experiment results of the relative gene expression level (24 hours) of the Jieshou fruit membrane extract. Figure 17 is the bar chart of the cell experiment results of the relative gene expression level (48 hours) of the Jieshou fruit membrane extract. Figure 18 is the bar chart of the cell experiment results of the relative gene expression level (48 hours) of the Jieshou fruit membrane extract. Figure 19 is the bar chart of the human experiment results of the malondialdehyde concentration, an antioxidant index in the blood, at week 0, week 8, and week 12. Figure 20 is the bar chart of the human experiment results of the hair diameter measurement of the subjects at week 0, week 8, and week 12. TW202446406A_113118983_SEQL.xml G01: Blank group G02: Control group G30, G32: Experimental groups

Claims

1. Use of an Anacardium occidentale membrane extract for the preparation of a scalp care composition, wherein the Anacardium occidentale membrane extract is obtained by extracting an Anacardium occidentale membrane.

2. The use as claimed in claim 1, wherein in the extraction step, the solid-liquid ratio of the euphorbia humifusa membrane to the solvent is 5 to 20:1 to 5, the extraction temperature is 80°C to 90°C, and the extraction time is 80 minutes to 120 minutes.

3. The use as described in claim 1, wherein the euphorbia humifusa extract has the ability to increase hair follicle health.

4. The use as described in claim 3, wherein the hair follicle extract increases hair follicle health by enhancing the antioxidant capacity of hair follicle cells.

5. The use as claimed in claim 4, wherein the mesona chinensis extract is at least one compound selected from the group consisting of epicatechin coumarin ester, phloroglucinol, protocatechuic acid and naringenin 7-O-(6”-O-p-coumaryl)-β-D-glucoside.

6. The use as claimed in claim 3, wherein the euphorbia humifusa extract increases hair follicle health by promoting hair follicle cell proliferation.

7. The use as claimed in claim 6, wherein the mesona chinensis extract is at least one compound selected from the group consisting of epicatechin coumarate, phloroglucinol, protocatechuic acid and naringenin 7-O-(6”-O-p-coumaryl)-β-D-glucoside.

8. The use as described in claim 3, wherein the hair follicle extract increases hair follicle health by increasing angiogenesis.

9. The use as described in claim 3, wherein the hair follicle extract increases hair follicle health by promoting the expression of hair growth genes.

10. The use as claimed in claim 3, wherein the longevity fruit membrane extract increases hair follicle health by reducing the expression of hair loss genes.

11. The use as described in claim 3, wherein the hair follicle health is increased by reducing blood MDA concentration.

12. The use as claimed in claim 1, wherein the euphorbia humifusa extract has the ability to increase hair diameter.

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