A natural killer cell separation membrane and its coating layer composition
Patent Information
- Application Number
- TW114107829
- Authority / Receiving Office
- TW · TW
- Patent Type
- Patents
- Current Assignee / Owner
- Filing Date
- 2025-02-27
- Publication Date
- 2026-10-01
- Estimated Expiration
- 2045-02-26
Smart Images
Figure TWG2TB001913733_001 
Figure TWG2TB001913733_002 
Figure TWG2TB001913733_003
Abstract
Claims
1. A natural killer cell separation membrane comprising a coating and a substrate, the coating comprising a plurality of antibodies, the antibodies being anti-CD56 antibodies or biotin-labeled anti-CD56 antibodies, a plurality of streptavidins, and a polymer having a plurality of carboxylic acid groups, the polymer having a plurality of carboxylic acid groups comprising an acrylic hydrogel, polyacrylic acid, (acrylate-N-alkylacrylamide) copolymer, (acrylate-N-alkylacrylamide) copolymer network hydrogel, (acrylate-alkyl methacrylate) copolymer, (acrylate-alkyl methacrylate) copolymer network hydrogel, or a combination thereof, the plurality of antibodies binding the plurality of streptavidins via biotin and the plurality of streptavidins being chemically coupled to the polymer having a plurality of carboxylic acid groups, thereby forming the coating; and the coating being coated on the substrate, thereby constituting the natural killer cell separation membrane.
2. The natural killer cell separation membrane as claimed in claim 1, wherein the molar ratio of acrylic acid to N-alkylacrylamide in the acrylic acid-N-alkylacrylamide copolymer or the acrylic acid-N-alkylacrylamide copolymer network hydrogel is 1 to 20.
3. The natural killer cell separation membrane as claimed in claim 1, wherein the alkyl group of the (acrylic acid-alkyl methacrylate) copolymer and the (acrylic acid-alkyl methacrylate) copolymer network hydrogel is a linear alkyl group having 1 to 18 carbon atoms, a branched alkyl group having 3 to 18 carbon atoms, or a cyclic alkyl group having 3 to 8 carbon atoms.
4. The natural killer cell separation membrane as claimed in claim 1, wherein the chemical bond is selected from one or a combination of the following groups: amide bond, ester bond, acetal bond, ketal bond, ether bond, and disulfide bond.
5. The natural killer cell separation membrane as claimed in claim 1, wherein the Fourier transform infrared spectrum of the coating has absorption peaks in the following wavenumber ranges: 1545-1579 cm⁻¹, 1627-1654 cm⁻¹, 1710-1740 cm⁻¹, and 3000-3600 cm⁻¹.
6. A method for separating and recovering natural killer cells from a sample, comprising the following steps: contacting a sample containing natural killer cells with a natural killer cell separation membrane at 30-40°C, thereby adsorbing the natural killer cells onto the natural killer cell separation membrane; the natural killer cell separation membrane comprising a coating and a substrate, the coating comprising a plurality of antibodies, the antibodies being anti-CD56 antibodies or biotin-labeled anti-CD56 antibodies, a plurality of streptavidins, and a polymer having a plurality of carboxylic acid groups, the polymer having a plurality of carboxylic acid groups comprising an acrylic hydrogel, polyacrylic acid, (acrylate-N-alkylacrylamide) copolymer, (acrylate-N-alkylacrylamide) copolymer network hydrogel, (acrylate-alkyl methacrylate) copolymer... A polymer, a (acrylate-alkyl methacrylate) copolymer network hydrogel, or a combination thereof, wherein a plurality of antibodies bind to a plurality of streptavidin via biotin, and the plurality of streptavidin are chemically coupled to the polymer having a plurality of carboxylic acid groups, thereby forming the coating; and the coating is coated on the substrate, thereby constituting the natural killer cell separation membrane; performing a desorption process, incubating the natural killer cell separation membrane at 0-10°C for at least 10 minutes or immersing it in a solution containing biotin, thereby desorbing the natural killer cells adsorbed on the natural killer cell separation membrane; and performing a recovery process, rinsing the natural killer cell separation membrane with solution at 0-10°C, thereby recovering the desorbed natural killer cells, achieving the separation and recovery of natural killer cells from the sample.
7. The method for self-sample separation and recovery of natural killer cells as described in claim 6, wherein the acrylic acid-N-alkylacrylamide copolymer or the acrylic acid-N-alkylacrylamide copolymer network hydrogel has a molar ratio of acrylic acid to N-alkylacrylamide of 1 to 20.
8. The method for self-sample separation and recovery of natural killer cells as described in claim 6, wherein the alkyl group of the (acrylic acid-alkyl methacrylate) copolymer and (acrylic acid-alkyl methacrylate) copolymer network hydrogel is a linear alkyl group having 1 to 18 carbon atoms, a branched alkyl group having 3 to 18 carbon atoms, or a cyclic alkyl group having 3 to 8 carbon atoms.
9. The method for self-sampling and recovery of natural killer cells as described in claim 6, wherein the chemical bond is selected from one or a combination of the following groups: amide bond, ester bond, acetal bond, ketal bond, ether bond, and disulfide bond.
10. The method for self-sampled recovery of natural killer cells as described in claim 6, wherein the coating has absorption peaks in the Fourier transform infrared spectrum in the following wavenumber ranges: 1545-1579 cm⁻¹, 1627-1654 cm⁻¹, 1710-1740 cm⁻¹, and 3000-3600 cm⁻¹.