Honeysuckle extracellular vesicle solution, its pharmaceutical uses and preparation method

Abstract

This invention provides a honeysuckle extracellular vesicle solution comprising honeysuckle extracellular vesicles, wherein the concentration of the extracellular vesicles is from 1×10⁵ vesicles / ml (EV / ml) ​​to 1×10¹⁰ EV / ml. This invention also provides a method for preparing the honeysuckle extracellular vesicle solution and its pharmaceutical uses. The honeysuckle extracellular vesicle solution of this invention has skin care and hair growth promotion effects.

Claims

1. A honeysuckle extracellular vesicle solution comprising extracellular vesicles of honeysuckle (Lonicera japonica); wherein, The honeysuckle was fresh and did not show signs of drying or wilting, and the concentration of the extracellular vesicles was between 9 × 10⁷ vesicles / ml (EV / ml) ​​and 3 × 10⁹ EV / ml.

2. The honeysuckle extracellular vesicle solution as described in claim 1, wherein the honeysuckle is a flower.

3. The honeysuckle extracellular vesicle solution as described in claim 1, wherein the median particle size of the extracellular vesicles is 80 nanometers to 230 nanometers.

4. The honeysuckle extracellular vesicle solution as described in claim 1, comprising an excipient.

5. Use of a honeysuckle extracellular vesicle solution as described in any one of claims 1 to 4 for the preparation of skin care products.

6. The use as described in claim 5, wherein the skin care comprises either or a combination of promoting collagen production and reducing oxidative stress in the skin.

7. The use as described in claim 5, wherein the skin care comprises promoting the expression of genes that promote the expression of either or a combination of collagen and antioxidant enzymes.

8. The use as described in claim 6 or 7, wherein the collagen comprises type III collagen.

9. The use as described in claim 5, wherein the skin comprises any or a combination of dermal fibroblasts and keratinocytes.

10. Use of a honeysuckle extracellular vesicle solution as described in any one of claims 1 to 4 for the preparation of a hair growth promoting drug.

11. The use as described in claim 10, wherein the promotion of hair growth includes any or a combination of increasing hair volume, preventing hair loss, and treating hair loss.

12. The use as described in claim 10, wherein the promotion of hair growth comprises promoting the gene expression of any or a combination of vascular endothelial growth factor and antioxidant enzymes in the dermal papilla cells of the hair follicle.

13. Use of a honeysuckle extracellular vesicle solution as described in any one of claims 1 to 4 for the preparation of a wound-healing medicine.

14. A method for preparing a honeysuckle extracellular vesicle solution as described in any one of claims 1 to 4, comprising: a homogenization step: homogenizing the honeysuckle to obtain a honeysuckle homogenate; a purification step: sequentially subjecting the honeysuckle homogenate to a first filtration, a first centrifugation, a second centrifugation, and a second filtration to obtain a honeysuckle filtrate; an ultracentrifugation step: sequentially subjecting the honeysuckle filtrate to a first ultracentrifugation, a first reconstitution, a second ultracentrifugation, and a second reconstitution to obtain a mixture; and a sterilization step: subjecting the mixture to a third filtration to obtain the honeysuckle extracellular vesicle solution; wherein, The first filter has a pore size of 25 to 45 micrometers; the first centrifugation is performed at a speed of 700 × g to 1300 × g for 5 to 15 minutes; the second centrifugation is performed at a speed of 2000 × g to 3000 × g for 15 to 25 minutes; the second filter has a pore size of 0.1 to 0.22 micrometers; the first ultracentrifugation is performed at a speed of 1 × 10⁵ × g to 2 × 10⁵ × g for 2 to 4 hours; the second ultracentrifugation is performed at a speed of 1 × 10⁵ × g to 2 × 10⁵ × g for 40 to 80 minutes; and the third filter has a pore size of 0.1 to 0.22 micrometers.

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