Non-soy plant protein composition, its use for enhancing equol production efficiency and a method for producing equol.

Abstract

This invention provides a non-soybean-derived plant protein culture medium, which, after fermentation by a bacterial strain, can promote the production efficiency of equol. The non-soybean-derived plant protein culture medium contains the Lactiplantibacillus plantarum PH2 strain and kudzu root powder, and further contains one or a combination of pea protein, green pea flour, whey, lactose, and arginine. This invention also provides a method for preparing equol from the non-soybean-derived plant protein culture medium.

Claims

1. A non-soybean-derived plant protein culture medium comprising a *Lactiplantibacillus plantarum* PH2 strain, kudzu root powder, pea protein, green pea powder, whey, lactose, and arginine, wherein the *Lactiplantibacillus plantarum* PH2 strain is registered at NPMD (NITE Patent Microorganisms Depositary) / IPOD (International Patent Organism Depositary, NITE) in Japan, with registration number BP-04270, wherein the proportion of pea protein is 1-3%; the proportion of green pea powder is 1-3%; the proportion of kudzu root powder is 0.5-2%; the proportion of whey is 0.5-5%; the proportion of lactose is 0.5-3.2%; and the proportion of arginine is 0.5-3%.

2. The non-soybean-derived plant protein culture medium as described in claim 1, wherein the non-soybean-derived plant protein culture medium comprises 2% pea protein, 2% green pea flour, 0.5% kudzu root powder, 0.5% whey, 1.6% lactose and 1.5% arginine.

3. Use of a non-soybean-derived plant protein culture medium for promoting equol production efficiency, wherein the non-soybean-derived plant protein culture medium is any one of claims 1 to 2.

4. The use of the non-soybean-derived plant protein culture medium as described in claim 3 for improving equol conversion efficiency, wherein the non-soybean-derived plant protein culture medium contains 1-3% pea protein, 1-3% green pea flour, 0.5-2% kudzu root powder, 0.5-5% whey, 0.5-3.5% lactose, and 0.5-3% arginine.

5. The use of the non-soybean-derived plant protein culture medium as described in claim 3 for improving equol conversion efficiency, wherein the non-soybean-derived plant protein culture medium comprises 2% pea protein, 2% green pea flour, 0.5% kudzu root powder, 0.5% whey, 1.6% lactose and 1.5% arginine.

6. A method for preparing estrol, comprising: (A) spreading Lactiplantibacillus plantarum PH2 bacterial suspension onto a Gifu Anaerobic Agar plate, then placing the Gifu Anaerobic Agar plate in an anaerobic chamber and culturing it at 37°C for 1 day; finally, placing the Gifu Anaerobic plate into a Gifu Anaerobic Broth and culturing it at 37°C for 1 day in an anaerobic chamber to obtain activated Lactiplantibacillus plantarum PH2 bacterial suspension; adding 1-3% pea protein, 1-3% green pea flour, 0.5-2% kudzu root powder, 0.5-5% whey, and 0.5-3.2% estrol to a basal culture medium. Lactose and arginine (compared to 0.5-3% of the total culture medium) are added, followed by 4% of activated Lactiplantibacillus plantarum PH2 bacterial suspension (by total volume) to obtain a non-soybean-derived plant protein culture medium. This basal culture medium contains 0.25% daidzein (pea extract), 1.5% yeast extract, 0.55% NaCl, and 97.7% water. The Lactiplantibacillus plantarum PH2 strain is registered at NPMD (NITE Patent Microorganisms Depositary) / IPOD (International Patent Organism Depositary, NITE) in Japan, with registration number BP-04270. (B) Estrol is prepared by culturing the non-soybean-derived plant protein obtained in step (A) at 30-40°C, anaerobic conditions, and pH 5.5-6.5 for 120 hours.

7. A method for preparing equol as described in claim 6, wherein the pea protein is 2%; the green pea flour is 2%; the kudzu root powder is 2%; the whey is 0.5%; the lactose is 1.6%; and the arginine is 1.5%.

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