Use of ethyl acetate fraction of citrus depressa hayata peel

Abstract

This invention relates to the use of an ethyl acetate fraction of Taiwanese lemon peel, aiming to address the problems of high side effects and limited efficacy of conventional lipid-lowering drugs. The use involves extracting a crude extract from a Taiwanese lemon peel sample using an ethanol-water solution, drying it, suspending the crude extract in water, partitioning it with ethyl acetate, and then drying the ethyl acetate layer to obtain an ethyl acetate fraction of the Taiwanese lemon peel, which is used to prepare a lipid-lowering drug. This ethyl acetate fraction of Taiwanese lemon peel can lower the levels of total cholesterol, triglycerides, and lipoprotein cholesterol in the blood, while increasing the level of high-density lipoprotein cholesterol; it can also lower total cholesterol and triglycerides in the liver, thereby improving dyslipidemia and promoting cholesterol metabolism, achieving the effect of improving hyperlipidemia.

Claims

1. The use of an ethyl acetate extract from the peel of a Taiwanese lemon, specifically for the preparation of lipid-lowering drugs, wherein... The ethyl acetate fraction of Taiwanese lemon peel was prepared by a method comprising the following steps: providing a sample of Taiwanese lemon peel; extracting the sample of Taiwanese lemon peel with an aqueous ethanol solution to obtain a crude extract; drying the crude extract to remove the aqueous ethanol solution to obtain a crude extract; suspending the crude extract in water to obtain a suspension; adding ethyl acetate to the suspension for partition extraction to obtain an ethyl acetate layer solution; and drying the ethyl acetate layer solution to obtain the ethyl acetate fraction of Taiwanese lemon peel; wherein, the lipid-lowering drug is used to reduce the levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol in the blood, and to increase the level of high-density lipoprotein cholesterol.

2. The use of the ethyl acetate extract of Taiwan lemon peel as requested in claim 1, wherein, The particle size of the Taiwanese lemon peel sample ranged from 0.05 to 0.40 mm.

3. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, This ethanol-water solution is composed of 50-95% ethanol and 5-50% water by weight.

4. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, The crude extract was obtained by extracting the Taiwanese lemon peel sample with the ethanol aqueous solution at a temperature of 55-80°C for 2-4 hours.

5. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, The crude extract was dried at a temperature of 45–60°C and a pressure of 20–70 mmHg to obtain the crude extract.

6. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, Add 1–5 g of crude extract to every 100 mL of water to suspend the crude extract in water to obtain the suspension.

7. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, Add ethyl acetate at a ratio of 100-200 mL per 100 mL of suspension.

8. The use of the ethyl acetate extract from the peel of *Citrus reticulata* as requested in claim 1, wherein, Partition extraction was performed at a temperature of 25–35°C to obtain the ethyl acetate layer solution.

9. The use of the ethyl acetate extract of Taiwan lemon peel as requested in claim 1, wherein, The ethyl acetate layer solution was dried at a temperature of 45–50°C and a pressure of 20–70 mmHg to obtain the ethyl acetate fraction of Taiwan lemon peel.

10. The use of the ethyl acetate extract of Taiwan lemon peel as described in any of claims 1 to 9, wherein, The ethyl acetate extract of Taiwan lemon peel was administered orally to a single individual.

11. The use of the ethyl acetate extract of Taiwan lemon peel as described in any of claims 1 to 9, wherein, The ethyl acetate extract of Taiwan lemon peel was administered to a single individual at a dose of 15–75 mg / kg body weight / day.

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