Kit and method for detecting enantiomers of mandelic acid

Abstract

A kit for detecting mirror-isomers of mandelic acid is provided for use in detecting mirror-isomers of mandelic acid in a test sample. The kit comprises: a derivatizing reagent, linagliptin, for performing a coupling derivatization reaction with at least one mirror-isomer of mandelic acid in the test sample to form at least one mandelic acid derivative; an alkaline catalyst, N-methylmorpholine, for providing an alkaline environment for the coupling derivatization reaction; and a coupling reagent, 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolyl[4,5-B]pyridine 3-oxide hexafluorophosphate, for mediating the coupling derivatization reaction. The invention also relates to a method for detecting mirror-isomers of mandelic acid using the aforementioned kit. This allows for the differentiation of mirror-isomers of mandelic acid contained in the test sample.

Claims

1. A kit for detecting mirror isomers of mandelic acid, comprising: a derivatizing reagent, linagliptin, for performing a coupling derivatization reaction with at least one mirror isomer of mandelic acid in a test sample to form at least one mandelic acid derivative; a basic catalyst, N-methylmorpholine, for providing a basic environment for carrying out the coupling derivatization reaction; and a coupling reagent, 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolyl[4,5-B]pyridine 3-oxide hexafluorophosphate, for mediating the coupling derivatization reaction.

2. A method for detecting mirror isomers of mandelic acid, comprising: a sample providing step, comprising providing a sample containing at least one mirror isomer of mandelic acid; a coupling derivatization step, comprising mixing the sample, an alkaline catalyst, a coupling reagent, and a derivatization reagent, and, in an alkaline environment provided by the alkaline catalyst, causing the derivatization reagent to undergo a coupling derivatization reaction with the at least one mirror isomer of mandelic acid mediated by the coupling reagent to obtain a derivatization solution containing at least one mandelic acid derivative, wherein the alkaline catalyst is N-methylmorpholine, the coupling reagent is 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolyl[4,5-B]pyridine 3-oxide hexafluorophosphate, and the derivatization reagent is linagliptin; and a detection step, comprising detecting at least one mandelic acid derivative in the derivatization solution to obtain at least one signal wave.

3. The method for detecting mirror isomers of mandelic acid, as described in claim 2, wherein, In the sample provision step, the mirror isomer of at least one mandelic acid includes an R-type mandelic acid and an S-type mandelic acid. In the coupling derivatization step, the derivatizing reagent undergoes the coupling derivatization reaction with the R-type mandelic acid and the S-type mandelic acid respectively, and the at least one mandelic acid derivative includes an R-type mandelic acid derivative and an S-type mandelic acid derivative. In the detection step, the at least one signal wave includes an R-type signal wave and an S-type signal wave.

4. The method for detecting mirror isomers of mandelic acid, as described in claim 2 or 3, wherein, In this detection step, the derivatized solution is detected by high performance liquid chromatography to obtain at least one signal wave.

5. The method for detecting mirror isomers of mandelic acid as claimed in claim 4, further comprising a reaction termination step, which is performed after the coupling derivatization step and before the detection step, by adding an aqueous solution of phosphoric acid to the derivatization solution to terminate the continuation of the coupling derivatization reaction.

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